ISBxe2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007951 | ND | Burkholderia xenovorans | Burkholderia xenovorans LB400 |
DNA section
IS Length : 1287 bp
Ends
IR Length : 23/29
IRL : TGAGCTTGCCCCGGAAAAACGAATCCCTTGCTGAGCAGGTAAACTCAAGC
IRR : TGACCTTGCCCCTGTTTGACGAATCCCATAGCCACGGAAATCAGGCCGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCGGAAGAA | GAAATCTCCC | 0 |
DNA sequence
TGAGCTTGCCCCGGAAAAACGAATCCCTTGCTGAGCAGGTAAACTCAAGCAAGGAGAAGAACTATGACAAGCAAGGCAAAGCGAGCGCAGTACACGCTCG
AGTTCAAGCTGGAAGCGGTGCGACTGGTCAAAGCCGGGCAAAGCATGGCGGCGGTGGCGGCGACACTGAGTGTGGTTGAACAGACGCTGTACAACTGGGT
GAAGGCTGACCGGGAAGGCAAGCTGACGGGAGCCGGCACGAAGCCTGTGAGTCCGGAGCAGATGGAGCTGGCGCGGCTGCGTGCCGAAGTGGCTCGCCTG
AAGATGGAGCGAGATATTTTAAAAAAAGCAACCGCGTACTTCGCCAAGGAGTCGATGTGAAGTACGCGTTCATCGAGCGCAATCGACACCACTGGCCGGT
CTCGGTGCTATGTGAAGTGCTGGAAGTCAGTCCCAGCGGATATCATCAGCGCAGGCAACGCACCGCGCAGGCCAAGCCGCACCGAGGCCGCGTGAGTGAC
GACGCACTGCTTGCGCACATCAAGACGATTCACACGCAGGTCAGGGGCGAATACGGCTGGCCGCGCATGTGGAAGGAGCTGCTCACGCATGGCGTGCGCG
TGGGCAAGGAGCGGGTTCGCAAACTGATGGCACAGCATGGCATTCGAGCCCGGCACAAGCGCAAGTACATCGCGACGACAAACTCGAACCACGATTTGCC
GGTTGCGCCAAATCTGCTGGAGCGCAATTTCACTGCAACTGCACCGAACCAGGTCTGGACGACCGACATCACGTATCTGGCGACAGCCGAGGGCTGGGTG
TACCTCGCGGTCATCATCGACCTGTTCAGCCGGCAGGTGGTGGGCTGGTCGATGCAGCCGCACATGAAGGCTGAACTGGTCACGGACGCATTGCGCATGG
CGTGGTTCCGACGCCGCCCTGAAGCCGGCGTAATCGTGCATAGCGACAGGGGCAGCCAGTATTGCAGCGGCCTGTTTCAGGACACGTTGAAGGCCTATGG
CATGCGTTCGTCAATGAGCCGGCGCGGCGATTGTTGGGATAACGCGCCTACTGAAAGTCTATGGAGTTCGCTAAAGGTCGCACGTATGCATGGGCGGCAC
TTCGCCACGCGGCGTGCCGCAATGGATGAAGTGATTGATTGGCTAGGCTTTTACAATGCGCGGCGCCTACACTCGACGCTCAACTACGTCAGCCCCATGA
CGTTCGAGAAAAACTGGGCCGCAGTTCAGCGAGGCGAAGCGGCCTGATTTCCGTGGCTATGGGATTCGTCAAACAGGGGCAAGGTCA
AGTTCAAGCTGGAAGCGGTGCGACTGGTCAAAGCCGGGCAAAGCATGGCGGCGGTGGCGGCGACACTGAGTGTGGTTGAACAGACGCTGTACAACTGGGT
GAAGGCTGACCGGGAAGGCAAGCTGACGGGAGCCGGCACGAAGCCTGTGAGTCCGGAGCAGATGGAGCTGGCGCGGCTGCGTGCCGAAGTGGCTCGCCTG
AAGATGGAGCGAGATATTTTAAAAAAAGCAACCGCGTACTTCGCCAAGGAGTCGATGTGAAGTACGCGTTCATCGAGCGCAATCGACACCACTGGCCGGT
CTCGGTGCTATGTGAAGTGCTGGAAGTCAGTCCCAGCGGATATCATCAGCGCAGGCAACGCACCGCGCAGGCCAAGCCGCACCGAGGCCGCGTGAGTGAC
GACGCACTGCTTGCGCACATCAAGACGATTCACACGCAGGTCAGGGGCGAATACGGCTGGCCGCGCATGTGGAAGGAGCTGCTCACGCATGGCGTGCGCG
TGGGCAAGGAGCGGGTTCGCAAACTGATGGCACAGCATGGCATTCGAGCCCGGCACAAGCGCAAGTACATCGCGACGACAAACTCGAACCACGATTTGCC
GGTTGCGCCAAATCTGCTGGAGCGCAATTTCACTGCAACTGCACCGAACCAGGTCTGGACGACCGACATCACGTATCTGGCGACAGCCGAGGGCTGGGTG
TACCTCGCGGTCATCATCGACCTGTTCAGCCGGCAGGTGGTGGGCTGGTCGATGCAGCCGCACATGAAGGCTGAACTGGTCACGGACGCATTGCGCATGG
CGTGGTTCCGACGCCGCCCTGAAGCCGGCGTAATCGTGCATAGCGACAGGGGCAGCCAGTATTGCAGCGGCCTGTTTCAGGACACGTTGAAGGCCTATGG
CATGCGTTCGTCAATGAGCCGGCGCGGCGATTGTTGGGATAACGCGCCTACTGAAAGTCTATGGAGTTCGCTAAAGGTCGCACGTATGCATGGGCGGCAC
TTCGCCACGCGGCGTGCCGCAATGGATGAAGTGATTGATTGGCTAGGCTTTTACAATGCGCGGCGCCTACACTCGACGCTCAACTACGTCAGCCCCATGA
CGTTCGAGAAAAACTGGGCCGCAGTTCAGCGAGGCGAAGCGGCCTGATTTCCGTGGCTATGGGATTCGTCAAACAGGGGCAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 64 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MTSKAKRAQYTLEFKLEAVRLVKAGQSMAAVAATLSVVEQTLYNWVKADREGKLTGAGTKPVSPEQMELARLRAEVARLKMERDILKKATAYFAKESM
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1038 bp | 345 aa | 210 | 1247 | + | No |
Description : Second part of the transposase
ORF sequence :
PGRQADGSRHEACESGADGAGAAACRSGSPEDGARYFKKSNRVLRQGVDVKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDAL
LAHIKTIHTQVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLA
VIIDLFSRQVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSQYCSGLFQDTLKAYGMRSSMSRRGDCWDNAPTESLWSSLKVARMHGRHFAT
RRAAMDEVIDWLGFYNARRLHSTLNYVSPMTFEKNWAAVQRGEAA
LAHIKTIHTQVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLA
VIIDLFSRQVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSQYCSGLFQDTLKAYGMRSSMSRRGDCWDNAPTESLWSSLKVARMHGRHFAT
RRAAMDEVIDWLGFYNARRLHSTLNYVSPMTFEKNWAAVQRGEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1184 bp | 394 aa | 64 | 1247 | + | Yes |
Chemistry : DDE
ORF sequence :
MTSKAKRAQYTLEFKLEAVRLVKAGQSMAAVAATLSVVEQTLYNWVKADREGKLTGAGTKPVSPEQMELARLRAEVARLKMERDILKKSNRVLRQGVDVK
YAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHTQVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKR
KYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSQY
CSGLFQDTLKAYGMRSSMSRRGDCWDNAPTESLWSSLKVARMHGRHFATRRAAMDEVIDWLGFYNARRLHSTLNYVSPMTFEKNWAAVQRGEAA
YAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHTQVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKR
KYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSQY
CSGLFQDTLKAYGMRSSMSRRGDCWDNAPTESLWSSLKVARMHGRHFATRRAAMDEVIDWLGFYNARRLHSTLNYVSPMTFEKNWAAVQRGEAA
Blast result :
Comments
ISBxe2 is 84 % (ORFA) aa similar to ISPsy28 and 78% (ORFB) aa similar to ISRasp1.
The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Lykidis,A. and Richardson,P. (2006) Direct submission GenBank.