ISDde3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007519 | ND | Desulfovibrio desulfuricans | Desulfovibrio desulfuricans G20 |
DNA section
IS Length : 1278 bp
Ends
IR Length : 31/40
IRL : TGATCCTGCTACAAAGTTGTGGACACAGAGTTAAGCTACTATCCTGCCTA
IRR : TGATCCTGCCACATTTTTGTGGAGTACCAGTTAAGCTACTTTTTTGTATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGCAAGGCA | CTT | TATAGCCAAA | 3 |
DNA sequence
TGATCCTGCTACAAAGTTGTGGACACAGAGTTAAGCTACTATCCTGCCTACGAAGGAGATATGGATGAGTAGCCCAAAACGTCGCACTTATGATTCAGAA
TTCAAACAAAACGCCGTGTTATTAACAGAAGACCCCAGCCGCACAGTAACCGAGGTTGCTGAAAGTCTGGGAGTTGCGAAAGACCTTCTTTATCGGTGGC
GAAGAGAATTCGCAGCGCAAGGTAAGGTGGCCTTTTCAGGCCGAGGGAATCCGGCCCTCACAGATGAACAGCGCCGTATTCGGGAACTGGAAAAGAAGCT
TAAAAATGCAGAATTGGAGCGGGATATTTTAAAAAAAGCCTTGGCCATCTTCAGCAGCGCACCGAAATAAAATTTCAAATTATCAAAAATTGCCGCTCGT
TATTTCCGGTGAAGAAGATGTGCCATATGCTCGGGGTTTCCCAGAGCGGCTTTTACTCGTGGGCGACTCGTCCCCCGTCTCAAAGGGCAAGGCATAATGC
TCAATTGCGCGTCCGTATCAGAGAATTGTTCGAGGAACACAAAAGAACAGCTGGAAGTCCGATGATTACAGAAGACTTACGGGCTGAAGACGCTTTTCGG
CATGTTAGTAGATCTCGGGTGGCTAGGCACATGCGGGAAATGGGGTTGCGCTGCCAGACCATGAAGAAGTTTGTTGTCACGACCGACTCCTCTCACTCTG
AGCCTGTTGCCCCGAATGTCCTGAATCGGCGGTTTTCTGCCTCAGCTCCAAACGTCACGTGGGTCAGCGACATTACTTACATAAAAATAGGCAGAACGTG
GCATTACCTGACGGTATTCATCGACCTGTATTCCCGCTTAGTTGTGGGGTGGGATCTCAGTGCATCGCTGGAGCGGCATTCTGTTATTCACGCGTTTCGG
AAAGCAGTGGCCCGTCGAAGGCCTTCAAAAGGGTTGCTTGTCCATAGCGATCGTGGAATCCAATATGCCAGTAGGGATTTCAGAAAGGAATTACACCGAC
ATGGCTGTATTCAAAGCATGAGTCGAAAAGGCAACTGTTGGGACAATGCCGTTGCTGAATCATTCTTCCATACCCTGAAAACCCAGTTGATCTATCAAAG
GAAATTTACAGACAGGCGTGAAGCAGAGCTTGCTCTCTTCCATTACATAGAGGCGTATTACAATCGTCGTCGGAGGCATTCCAGTAACGGTTGGATGTCT
CCTGCGGCTTTCGAAAATTTCAACCAGGAATACAAAAAAGTAGCTTAACTGGTACTCCACAAAAATGTGGCAGGATCA
TTCAAACAAAACGCCGTGTTATTAACAGAAGACCCCAGCCGCACAGTAACCGAGGTTGCTGAAAGTCTGGGAGTTGCGAAAGACCTTCTTTATCGGTGGC
GAAGAGAATTCGCAGCGCAAGGTAAGGTGGCCTTTTCAGGCCGAGGGAATCCGGCCCTCACAGATGAACAGCGCCGTATTCGGGAACTGGAAAAGAAGCT
TAAAAATGCAGAATTGGAGCGGGATATTTTAAAAAAAGCCTTGGCCATCTTCAGCAGCGCACCGAAATAAAATTTCAAATTATCAAAAATTGCCGCTCGT
TATTTCCGGTGAAGAAGATGTGCCATATGCTCGGGGTTTCCCAGAGCGGCTTTTACTCGTGGGCGACTCGTCCCCCGTCTCAAAGGGCAAGGCATAATGC
TCAATTGCGCGTCCGTATCAGAGAATTGTTCGAGGAACACAAAAGAACAGCTGGAAGTCCGATGATTACAGAAGACTTACGGGCTGAAGACGCTTTTCGG
CATGTTAGTAGATCTCGGGTGGCTAGGCACATGCGGGAAATGGGGTTGCGCTGCCAGACCATGAAGAAGTTTGTTGTCACGACCGACTCCTCTCACTCTG
AGCCTGTTGCCCCGAATGTCCTGAATCGGCGGTTTTCTGCCTCAGCTCCAAACGTCACGTGGGTCAGCGACATTACTTACATAAAAATAGGCAGAACGTG
GCATTACCTGACGGTATTCATCGACCTGTATTCCCGCTTAGTTGTGGGGTGGGATCTCAGTGCATCGCTGGAGCGGCATTCTGTTATTCACGCGTTTCGG
AAAGCAGTGGCCCGTCGAAGGCCTTCAAAAGGGTTGCTTGTCCATAGCGATCGTGGAATCCAATATGCCAGTAGGGATTTCAGAAAGGAATTACACCGAC
ATGGCTGTATTCAAAGCATGAGTCGAAAAGGCAACTGTTGGGACAATGCCGTTGCTGAATCATTCTTCCATACCCTGAAAACCCAGTTGATCTATCAAAG
GAAATTTACAGACAGGCGTGAAGCAGAGCTTGCTCTCTTCCATTACATAGAGGCGTATTACAATCGTCGTCGGAGGCATTCCAGTAACGGTTGGATGTCT
CCTGCGGCTTTCGAAAATTTCAACCAGGAATACAAAAAAGTAGCTTAACTGGTACTCCACAAAAATGTGGCAGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 65 | 370 | + | No |
Description : First part of the transposase
ORF sequence :
MSSPKRRTYDSEFKQNAVLLTEDPSRTVTEVAESLGVAKDLLYRWRREFAAQGKVAFSGRGNPALTDEQRRIRELEKKLKNAELERDILKKALAIFSSAP
K
K
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 304 | 1248 | + | No |
Description : Second part of the transposase
ORF sequence :
KCRIGAGYFKKSLGHLQQRTEIKFQIIKNCRSLFPVKKMCHMLGVSQSGFYSWATRPPSQRARHNAQLRVRIRELFEEHKRTAGSPMITEDLRAEDAFRH
VSRSRVARHMREMGLRCQTMKKFVVTTDSSHSEPVAPNVLNRRFSASAPNVTWVSDITYIKIGRTWHYLTVFIDLYSRLVVGWDLSASLERHSVIHAFRK
AVARRRPSKGLLVHSDRGIQYASRDFRKELHRHGCIQSMSRKGNCWDNAVAESFFHTLKTQLIYQRKFTDRREAELALFHYIEAYYNRRRRHSSNGWMSP
AAFENFNQEYKKVA
VSRSRVARHMREMGLRCQTMKKFVVTTDSSHSEPVAPNVLNRRFSASAPNVTWVSDITYIKIGRTWHYLTVFIDLYSRLVVGWDLSASLERHSVIHAFRK
AVARRRPSKGLLVHSDRGIQYASRDFRKELHRHGCIQSMSRKGNCWDNAVAESFFHTLKTQLIYQRKFTDRREAELALFHYIEAYYNRRRRHSSNGWMSP
AAFENFNQEYKKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1184 bp | 405 aa | 65 | 1248 | + | Yes |
Chemistry : DDE
ORF sequence :
MSSPKRRTYDSEFKQNAVLLTEDPSRTVTEVAESLGVAKDLLYRWRREFAAQGKVAFSGRGNPALTDEQRRIRELEKKLKNAELERDILKKALAIFSSAP
KKSLGHLQQRTEIKFQIIKNCRSLFPVKKMCHMLGVSQSGFYSWATRPPSQRARHNAQLRVRIRELFEEHKRTAGSPMITEDLRAEDAFRHVSRSRVARH
MREMGLRCQTMKKFVVTTDSSHSEPVAPNVLNRRFSASAPNVTWVSDITYIKIGRTWHYLTVFIDLYSRLVVGWDLSASLERHSVIHAFRKAVARRRPSK
GLLVHSDRGIQYASRDFRKELHRHGCIQSMSRKGNCWDNAVAESFFHTLKTQLIYQRKFTDRREAELALFHYIEAYYNRRRRHSSNGWMSPAAFENFNQE
YKKVA
KKSLGHLQQRTEIKFQIIKNCRSLFPVKKMCHMLGVSQSGFYSWATRPPSQRARHNAQLRVRIRELFEEHKRTAGSPMITEDLRAEDAFRHVSRSRVARH
MREMGLRCQTMKKFVVTTDSSHSEPVAPNVLNRRFSASAPNVTWVSDITYIKIGRTWHYLTVFIDLYSRLVVGWDLSASLERHSVIHAFRKAVARRRPSK
GLLVHSDRGIQYASRDFRKELHRHGCIQSMSRKGNCWDNAVAESFFHTLKTQLIYQRKFTDRREAELALFHYIEAYYNRRRRHSSNGWMSPAAFENFNQE
YKKVA
Blast result :
Comments
ISDde3 is 68 % (ORFA) aa similar to ISLbl1 and 67% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N. and Richardson,P.(2005) Direct submission GenBank.