ISBvi3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAEH02000049 | ND | Burkholderia vietnamiensis | Burkholderia vietnamiensis G4 |
DNA section
IS Length : 1282 bp
Ends
IR Length : 27/29
IRL : TGACCCTGCCTCGATTCCACGTACAGCAAGAAGTTTGATAACTTTTTGCC
IRR : TGACCCTGCCTCGTTTTCACGTACAGCAACTCATGCGGCGATCAAGCTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCATCCCAC | CG | CGCGGGATGC | 2 |
DNA sequence
TGACCCTGCCTCGATTCCACGTACAGCAAGAAGTTTGATAACTTTTTGCCTGTATGGAGAAGAACATGGCAGAGCACGTGGTACCCAGACGGCAGTACAC
GAAGGAATTCAAGGTTGAGGCGGTGCGGCTCGCTGAGAGCGTCGGTCAGCATGAAGCCGCTCGCAGGCTGGGCGTGCCGGTGGCGACGCTGGGGAACTGG
TGCCGTGATCAGCGCGATGGCAGTGCGCAGGCCGCAGCGGGGCCGGCGTCGAGTGCACCGAGCTCAAGTCGTCGTCCGGTTAGCGACCTGGAGGCCGAGA
ACAGCCGTCTGCGTAAGGAACTGGCCAGCGCAAAGCTGGATATCGAAATTCTTCGAAAGGCGACGGCGTACTTCGCGAAGGGGTCGCGGTGAGGTACGCC
TGGATTGACGCACATCGCGACCAGTACAGCGTAAGTCGGCTTTGCCGGGTTCTGGGTGTGTCGCGCAGCGGCTATTGCCAGTGGCGCGGCAGGGAGCCAA
GTCGGCGGGCACTGGCCAATGCCATGTTGGACGCTGAGGTCGCCGCCATTCATCGTGCCAGCCGCGGCAGCTATGGCCGTCCTCGAATCGTGCGGCAGTT
GCGTGCTCAGGGCCGGCCGGTGAGCGCCGAACGCGTGCGTCGCAGCCTGCAACGCCAGGGGCTACGTCCGGTCTACAAACGCCCGTATCGGGTGACGACG
GATTCGACGCATCGCTTGCCGGTGGCGCCGAATCTGTTGGATCGGCGTTTCGACGGCTGGCGGCCCAATCAAGCCTGGGTAAGCGACATCACGTTCGTGA
GGACCGGCGAGGGCTGGTTATATCTGGCGGCGATCCTGGATCTGGCGAGCCGGCGCATCGTCGGCTGGTCGATGTCCGAGCGGATCAACGCGGATCTGGT
CTGCCAGGCGTTGCGCAGCGCGTGCTGGCAACGCAAACCGCCACCGGGATTGCTGCTGCACTCCGATCTCGGCGCCCAATATGCCAGTCGCGCGTACCGG
AAATTGACCGCCGCGTTCAAGATAAACGTTTCAATGAGCCGCCGTGCCAACGCCTGGGATAACGCCCCGATGGAAAGCTTCTTCAAGACGCTGAAGGTCG
AAAGAATCTACCAGACTCACGACGAAACGCGTGCTCACGCCCGCCTGGATATCGTCGATTGGATCGAGGGCTATTACAATCGGCAGCGCCTGCATACCTC
GATCGACTTCCTTACCCCCGTCGACTACGAGGCTAGCTTGATCGCCGCATGAGTTGCTGTACGTGAAAACGAGGCAGGGTCA
GAAGGAATTCAAGGTTGAGGCGGTGCGGCTCGCTGAGAGCGTCGGTCAGCATGAAGCCGCTCGCAGGCTGGGCGTGCCGGTGGCGACGCTGGGGAACTGG
TGCCGTGATCAGCGCGATGGCAGTGCGCAGGCCGCAGCGGGGCCGGCGTCGAGTGCACCGAGCTCAAGTCGTCGTCCGGTTAGCGACCTGGAGGCCGAGA
ACAGCCGTCTGCGTAAGGAACTGGCCAGCGCAAAGCTGGATATCGAAATTCTTCGAAAGGCGACGGCGTACTTCGCGAAGGGGTCGCGGTGAGGTACGCC
TGGATTGACGCACATCGCGACCAGTACAGCGTAAGTCGGCTTTGCCGGGTTCTGGGTGTGTCGCGCAGCGGCTATTGCCAGTGGCGCGGCAGGGAGCCAA
GTCGGCGGGCACTGGCCAATGCCATGTTGGACGCTGAGGTCGCCGCCATTCATCGTGCCAGCCGCGGCAGCTATGGCCGTCCTCGAATCGTGCGGCAGTT
GCGTGCTCAGGGCCGGCCGGTGAGCGCCGAACGCGTGCGTCGCAGCCTGCAACGCCAGGGGCTACGTCCGGTCTACAAACGCCCGTATCGGGTGACGACG
GATTCGACGCATCGCTTGCCGGTGGCGCCGAATCTGTTGGATCGGCGTTTCGACGGCTGGCGGCCCAATCAAGCCTGGGTAAGCGACATCACGTTCGTGA
GGACCGGCGAGGGCTGGTTATATCTGGCGGCGATCCTGGATCTGGCGAGCCGGCGCATCGTCGGCTGGTCGATGTCCGAGCGGATCAACGCGGATCTGGT
CTGCCAGGCGTTGCGCAGCGCGTGCTGGCAACGCAAACCGCCACCGGGATTGCTGCTGCACTCCGATCTCGGCGCCCAATATGCCAGTCGCGCGTACCGG
AAATTGACCGCCGCGTTCAAGATAAACGTTTCAATGAGCCGCCGTGCCAACGCCTGGGATAACGCCCCGATGGAAAGCTTCTTCAAGACGCTGAAGGTCG
AAAGAATCTACCAGACTCACGACGAAACGCGTGCTCACGCCCGCCTGGATATCGTCGATTGGATCGAGGGCTATTACAATCGGCAGCGCCTGCATACCTC
GATCGACTTCCTTACCCCCGTCGACTACGAGGCTAGCTTGATCGCCGCATGAGTTGCTGTACGTGAAAACGAGGCAGGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 112 aa | 54 | 392 | + | No |
Description : First part of the transposase
ORF sequence :
MEKNMAEHVVPRRQYTKEFKVEAVRLAESVGQHEAARRLGVPVATLGNWCRDQRDGSAQAAAGPASSAPSSSRRPVSDLEAENSRLRKELASAKLDIEIL
RKATAYFAKGSR
RKATAYFAKGSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1236 bp | 311 aa | 17 | 1252 | + | No |
Description : Second part of the transposase
ORF sequence :
GTGQRKAGYRNSSKGDGVLREGVAVRYAWIDAHRDQYSVSRLCRVLGVSRSGYCQWRGREPSRRALANAMLDAEVAAIHRASRGSYGRPRIVRQLRAQGR
PVSAERVRRSLQRQGLRPVYKRPYRVTTDSTHRLPVAPNLLDRRFDGWRPNQAWVSDITFVRTGEGWLYLAAILDLASRRIVGWSMSERINADLVCQALR
SACWQRKPPPGLLLHSDLGAQYASRAYRKLTAAFKINVSMSRRANAWDNAPMESFFKTLKVERIYQTHDETRAHARLDIVDWIEGYYNRQRLHTSIDFLT
PVDYEASLIAA
PVSAERVRRSLQRQGLRPVYKRPYRVTTDSTHRLPVAPNLLDRRFDGWRPNQAWVSDITFVRTGEGWLYLAAILDLASRRIVGWSMSERINADLVCQALR
SACWQRKPPPGLLLHSDLGAQYASRAYRKLTAAFKINVSMSRRANAWDNAPMESFFKTLKVERIYQTHDETRAHARLDIVDWIEGYYNRQRLHTSIDFLT
PVDYEASLIAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1199 bp | 399 aa | 54 | 1252 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKNMAEHVVPRRQYTKEFKVEAVRLAESVGQHEAARRLGVPVATLGNWCRDQRDGSAQAAAGPASSAPSSSRRPVSDLEAENSRLRKELASAKLDIEIL
RKGDGVLREGVAVRYAWIDAHRDQYSVSRLCRVLGVSRSGYCQWRGREPSRRALANAMLDAEVAAIHRASRGSYGRPRIVRQLRAQGRPVSAERVRRSLQ
RQGLRPVYKRPYRVTTDSTHRLPVAPNLLDRRFDGWRPNQAWVSDITFVRTGEGWLYLAAILDLASRRIVGWSMSERINADLVCQALRSACWQRKPPPGL
LLHSDLGAQYASRAYRKLTAAFKINVSMSRRANAWDNAPMESFFKTLKVERIYQTHDETRAHARLDIVDWIEGYYNRQRLHTSIDFLTPVDYEASLIAA
RKGDGVLREGVAVRYAWIDAHRDQYSVSRLCRVLGVSRSGYCQWRGREPSRRALANAMLDAEVAAIHRASRGSYGRPRIVRQLRAQGRPVSAERVRRSLQ
RQGLRPVYKRPYRVTTDSTHRLPVAPNLLDRRFDGWRPNQAWVSDITFVRTGEGWLYLAAILDLASRRIVGWSMSERINADLVCQALRSACWQRKPPPGL
LLHSDLGAQYASRAYRKLTAAFKINVSMSRRANAWDNAPMESFFKTLKVERIYQTHDETRAHARLDIVDWIEGYYNRQRLHTSIDFLTPVDYEASLIAA
Blast result :
Comments
Genome in progress.
ISBvi3 is 62 % (ORFA) aa similar to ISGlo1 and 65% (ORFB) aa similar to ISBps1.The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
ISBvi3 is 62 % (ORFA) aa similar to ISGlo1 and 65% (ORFB) aa similar to ISBps1.The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank.