ISUnCu9

  • Family IS4
  • Group IS50
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND uncultured bacterium
uncultured bacterium
DNA section
IS Length : 1486 bp

Ends


IR Length : 16/20

IRL : CTGTCTCTTATACACAAATCCCTACTAAGTAATTAGTGGGGATTTTTTTG
IRR : CTGTCTCTTGATCACAAGTCGATATCAAGAGACAGGGCTAGCTCATAGCC

Insertion site


Left flankDirect repeatRight flankDR Length
AACAAAGCCGCCATATATTGGCGGCTTTTTT11

DNA sequence

CTGTCTCTTATACACAAATCCCTACTAAGTAATTAGTGGGGATTTTTTTGAACTCTTCCTTCCAACTCTGATCAGATCTTTCACTTAACTCATTGGGAAA
GATTATGATTGATAATGTAGAGTGGGCTGAAAAAACGTTTGGAGAAGCAAATCTAGGAGATCCTCGTCGTACATCTCGTCTGGTAAAATTAGCTGCAACA
CTTGCCAATACACCAGGAAAGCCTCTTGTGAATATCACTGAATCCCCCGCCGATATGGAAGGAGCATACCGCTTCATTAGAAATGAAAGTGTAGATGCGA
CTTCAATCGCTGAAACTGGATTTAACGTTACAGCTGTACAAGCTGCGCAACACAAAACATTGCTTGCCCTAGAAGATACTACGACCATTTCATACAGCCA
TAAAAGTATTCGAAACGAACTTGGACACGTGAATCAGGGTAACCGTTGCCGCGGTATGCTTGCCCATAGTGTCTTACTTTTTGCGCCGGAATCACAAGAA
ATAGTTGGACTGATTGAACAGTCGCGTTGGACACGAGACATAACAACGCGTGGTAAAAGAGCGAAACATGCATCGACACCATACACTGAGAAAGAAGGCT
ACAAGTGGGAGTCAGCATCAATCAATATGTCAGCAAGACTGGGCGCAAAGATAGATGATGTGATATCCGTCTGTGACCGAGAAGCCGATATCTACGAGTA
CCTACTTTATAAGCTAACAACTCAGCAGCGGTTTATTGTTCGTTCAATGCAAAGTCGGCACATTGAGGAAGGCGAAAACAAGCTATATCACTATGCCAGT
CAGTTAAAAAGTGCAGGGCACAAGCAAATACATATTCCGCAAAAAGGAGGGCGCAAAGCACGAAACGTGACGTTAGATATTGTATTTGCTCCCGTTACGC
TCAAGGTTCCTAGCAATAAACGTGGTGAATCACTTCCTTTATACTACGTCGGATGTGTAGAGCGCGGTAATTCAAAAGAGGCGTTATGCTGGCATTTACT
GACAAATGAACCCATCACAAATCAAGCGCAAGCACGAAAAATAATTGGCTATTACGAGCATCGTTGGCTAGTAGAAGAATATCATAAGGCGTGGAAAAGT
GACGGTACTAATATCGAAGCTTCGCGCTTACAAAGTAAGGAAAACGTTGAGCGCCTAGTCACAATAAACGCGTTTATTGCCGTAAGGATCCTTCAACTCA
AATTCGCAAAAGACCGACCGGACGATAGTAGCTGTGAAGAAGTCCTATCACCTAAAGCATGGAAGTTATTATGGTTAAAACGAGTGAGTAAGACACCACC
TGACTCAGCCCCCTCAATGAAATGGGCCTATACGGAGCTTGCAAAGCTAGGAGGGTGGAAAGATACAAAAAGAACAGGAAGAGCGTCTGTGAAAGTCATA
TGGCAAGGATGGTTCAAACTGCAAACCATCCTTGAAGGCTATGAGCTAGCCCTGTCTCTTGATATCGACTTGTGATCAAGAGACAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1368 bp456 aa1051472+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDNVEWAEKTFGEANLGDPRRTSRLVKLAATLANTPGKPLVNITESPADMEGAYRFIRNESVDATSIAETGFNVTAVQAAQHKTLLALEDTTTISYSHK
SIRNELGHVNQGNRCRGMLAHSVLLFAPESQEIVGLIEQSRWTRDITTRGKRAKHASTPYTEKEGYKWESASINMSARLGAKIDDVISVCDREADIYEYL
LYKLTTQQRFIVRSMQSRHIEEGENKLYHYASQLKSAGHKQIHIPQKGGRKARNVTLDIVFAPVTLKVPSNKRGESLPLYYVGCVERGNSKEALCWHLLT
NEPITNQAQARKIIGYYEHRWLVEEYHKAWKSDGTNIEASRLQSKENVERLVTINAFIAVRILQLKFAKDRPDDSSCEEVLSPKAWKLLWLKRVSKTPPD
SAPSMKWAYTELAKLGGWKDTKRTGRASVKVIWQGWFKLQTILEGYELALSLDIDL

 

Blast result :
Comments
ISUnCu9 is 63% aa similar to ISPpr3. ISUnCu9 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS50 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-132-E(C1). ACCESSION AACY01103432.
ISUnCu9 is from uncultured bacterium (environmental sequence from Sargasso Sea).
References
1] Venter,J.C., Remington,K., Heidelberg,J.F., Halpern,A.L., Rusch,D., Eisen,J.A., Wu,D., Paulsen,I., Nelson,K.E., Nelson,W., Fouts,D.E., Levy,S., Knap,A.H., Lomas,M.W., Nealson,K., White,O., Peterson,J., Hoffman,J., Parsons,R., Baden-Tillson,H., Pfannkoch,C., Rogers,Y.H. and Smith,H.O. (2004) Science 304 (5667), 66-74
2] Reznikoff, W.S., Bordenstein, S.R. and Apodaca, J. (2004) J. Bact. 186(24), 8240-8247
3] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18