ISRm31

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DQ145546 ND Sinorhizobium meliloti
Sinorhizobium meliloti
DNA section
IS Length : 1426 bp

Ends


IR Length : 20

IRL : GCCTAGCACTACAGTTGCATATTTATCTATGATCTGAATCTATGGGTCTC
IRR : GCCTAGCACTACAGTTGCATTTTAGTTTTGAGGTGAGCTCGTCGAGCGGC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GCCTAGCACTACAGTTGCATATTTATCTATGATCTGAATCTATGGGTCTCCATGATTCGGAGGTCATGGATGGCAGGTGCATCAATCGAGACGACGCTTG
AGCTTTGGGCATCATCGTTGCGCGACGTGAAGGCTCGCATGCGCGGACTGTTTACGCAGGAGCGAGTTGCAGCCTCTGCGAACCTTTTCCTGGACGGCTT
GCTGGGTGACGAGCGGCGTAAGACAGGTTGGATGCGTGCTGAGGCGGCCGGTGATCCCGGCCCGTGGCGGCAACAAGCCATTCTGGGGCGCGGGCGCTGG
GACGCGGACGCACTTCGCGACATCGTGCGAGAGTATGTCGTAGAAAACCTCGCCACGGATGATGCGGTCCTGGTCATCGATGAGACGGGCTTCCTCAAGC
AGGGCAAGACATCGTGCGGTGTTGCACGTCAATATACAGGTTTGGCTGGCAAGATAACGAACTGCCAGATCGGTGTGTTCGCCGCCTATGTGTCCGATCG
CGGCCATGCCTTTATCGATCGAGCCCTGTACTTGCCCAAGAGCTGGACTGGCGATCCGGCAAGGCTTGCAGCAGCTCATGTTCCTCAGGCTACAGCCTTC
GCTACCAAGCCAGGCCTGGCTGTCGAGATGATACGGCGTGCGCTGGCAGCCAATGTGCCGTTTTCATGGGTGGCCGCAGATGCGGTCTATGGCGTCGGGG
ACATCGAAGGAACCCTGCGCCGAGCCTGCAAAGGCTACGTTCTTGGGGTTAAATCGGACCACCATTTCGGCTCGTGGTCGGGAAAGCCTCCGGTCGCCGG
CACAGCGCAGGAGATCGCCCGTGATCTCGATCCAAGTGCATGGCAGCGTCTTTCCGCCGGTGAGGGCACCAAAGGCGCTCGGCTTCATGACTGGGCCTAT
TGCGAACTCGCCGACCTCGATGCCGACGAATACAACGAGACGAAATCTGGGCTTTGGACCCGTGGCCTCCTGATCCGGCGCAATATCAGCGACGGTGATC
TCGCATTCTTCACCACATGGTGCCCGGCCGGGACGGACATCCAGACGCTCGTTTCCGTTGAAGGCCATCGCTGGGCGATCGAAGACAGCTTCGAGACCGC
CAAGAACGAGCTCGGACTCGATCACAACGAAACCCGGTCATGGCATGGCTGGCATCGCCACGTCTCCCTCGTCATGCTCGCCTTCGCCATGATGGCGGTG
ATCCGATACCGCGCCAATGACGCGACGCGCCAAAAAAGACTGCGGATGCCGACCATCAGGATTTAATCCGCTGGTCTATCCAGGAAGTCCGACGCATCGC
CATAAGACTCGCTCAGCGTCGCATAAGCCCCGCCTACGTCATCGCATGGTCATGCTGGAGGCGCGCCCATCAGGCGGCCGCTCGACGAGCTCACCTCAAA
ACTAAAATGCAACTGTAGTGCTAGGC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1212 bp404 aa521263+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIRRSWMAGASIETTLELWASSLRDVKARMRGLFTQERVAASANLFLDGLLGDERRKTGWMRAEAAGDPGPWRQQAILGRGRWDADALRDIVREYVVENL
ATDDAVLVIDETGFLKQGKTSCGVARQYTGLAGKITNCQIGVFAAYVSDRGHAFIDRALYLPKSWTGDPARLAAAHVPQATAFATKPGLAVEMIRRALAA
NVPFSWVAADAVYGVGDIEGTLRRACKGYVLGVKSDHHFGSWSGKPPVAGTAQEIARDLDPSAWQRLSAGEGTKGARLHDWAYCELADLDADEYNETKSG
LWTRGLLIRRNISDGDLAFFTTWCPAGTDIQTLVSVEGHRWAIEDSFETAKNELGLDHNETRSWHGWHRHVSLVMLAFAMMAVIRYRANDATRQKRLRMP
TIRI

 

Blast result :
Comments
ISRm13 is 75% aa similar to IS1357. ISRm13 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS1452 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-133-E(C1). The copy number of ISRm13 in Sinorhizobium meliloti (plasmid pSmeSM11a) is 1.
References
1] Stiens,M., Schneiker,S., Keller,M., Kuhn,S., Puhler,A. and Schluter,A.(2006)Appl. Environ. Microbiol. 72 (5), 3662-3672
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18