ISBce4
- Family IS4
- Group IS231
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000040 | ND | Bacillus cereus | Bacillus cereus E33L |
DNA section
IS Length : 3340 bp
Ends
IR Length : 17
IRL : CATGCCCATCAAGCTAATATTTTGAGGCACCCCCAAAACAAAATTTTTAT
IRR : CATGCCCATCAAGCTAACCAAGTAATTTACTAGGTACGAAATGATCCTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATAGCAATG | GGGTAGCACCA | ACAAAACGCG | 11 |
DNA sequence
CATGCCCATCAAGCTAATATTTTGAGGCACCCCCAAAACAAAATTTTTATCTCTTGATAGTTATTTATGTTAATTCAGCTTGTTTTTTGAAGATTATCAG
TTTCTTTAACGGCATGACGATTTTATCTTATTCAGCCTTATTTAAATTTACTCGCAGGACAAATAAACCCTACATCTTTTGCGAACCTTTGAAGAATCTG
AGGGGATAAATGATGATGTAATTCCTGAGAGAATAGTTGAAACCCTTTTGCTAGTGAAATATCCATAAAAACACCATCCTTTTCATTGACCCTACTAAAG
GGAGAGTGTAAATGTTCTTTAGGAAATATATACTTAAGTAAATTAGCACTTATATTTTATTTGATAGTTATAGAAAAGCAAAAATAAAGATAAATTCTAC
ATTAAAAGAAGGATATTATTTTTTGTGTCGAATCAACTTATATAAGTATATAGTATTAGGTGACATGGGACAGTAGGAACCCATGTTGGCGTCATTTGTT
AAATGTAATTCCTTTTGCAGCGCAGTGCCTCCTTATACAAGGGGGCTTTTTTGTTGTAAATACATAATATATGGAGGCATAAGAGAGATGACAGACGAGT
ATAAACACTTAATTTCGTATGTAGATAAAATAAAAGAGCTTAATTATAGTACAGCTTCGGCATTAATAGTTATTCATAATGATAAAATTGTAGTAAACCA
TTATAACGGAAGGCATTCAAATGTTGCTTCATCAAAAAAGATCTCGCAATCATCACAATTCCATGTTGCTTCTGCACGTAAAAGTTACCTGGGTCTAGCG
GTTGCTTATGCCCTCTATGAGAGGAAAATTAAAAGTTTAGATGATTACGCAAGAGATTATTTTGATGAATATGATTGGGATGTTCTTGGGGATACCACTT
TAAGACATTTAGTGACATATTCTCATGGAATTGATGACAATGATGGAGCAATTTTTAGAGAATTTGGACCAGGAGAGCGTTGGGCGTATAAAGGAATGGG
GGTCATCATGATGACGAAACTCATTAACAACTTATACAAAAAGCCTTTTACACAATTACTTAAAGAAAGAGTTTTTGAGCCTCTTAATTTCAAAGAGACT
GCTTGGAGGACAATAGAGATCGATGAGCTTGTTAAAGTAATTACCGATCCCAGTAGGGATGCGATACTACCAATCAATCATTCAACAAGTGGGTTGGAAG
GGAATCTCTTTGTTTCTACAAGAGAGTTTGCATATTGGGGATATCTTCATTTAAAGAAAGGATTCATGAATGGAAAGCAAATAGTTCCTAAAGAAGTGAT
TGAAATTGCGACAAAAGTACAAAGTCCAATCTATAAAGACAGAACGCTCCCTGAAAATGGCCTATTTTGGTATGTCCAAAATGGCCATACTTTAAAAAGT
GAAATGGGTGAAAGGGTACCCGTAGGTTCATTCCAAATACTTGGAAACACAGGCCCGACTATCCTGGTAATTCCTAAATATAACGTGGTTGTAGCAAAAA
TGTATAATAAAAAATACAATTATGGAGGGGATAATTATCTTCATTACTTAAGAGAATTTAGCAATTTAGTTGCGGATACTTTTGCAAAATAATCTCATTT
ATTCAATTACTACTTAAAAACACAAAAACGTAATTACTTATTGTTTTACTGTTTTGGAGATACTTCAAAATTTAAGATTGATGGCTATTGGGTCACCATA
CATCATTATCAACCTCAGATAAATTTATATCCATAATTCACAGAAAACGTTATTCTTTTTGTAGAAATATACAGAAAGGATGGCGTTTTTGTATGAATCT
ATCGATTCAGGATGAGTTTCATTTGTTCGCTGAAGAGTTACAGCGATATCTATCTCCACATATTCTTCAACAACTCGCACAAGAGACAGGTTTTGTAAAA
CGTAAAAGTAAGTATGGCGCACGAGATTTGGCCGCTTTATGTATTTGGATTAGTCAACACGTAGCAAGCGATTCTCTTACTCGATTATGTAGTCAGCTTT
ATGCAAATACAGCTACACTTATGAGTCCAGAAGGACTGAATCAACGCTTTAATCCAGCAGCTGTCACATTTCTTCGAGAAGTCTTTACTTCTCTTCTCAC
ACAAAAACTCTGTTCAAATCACTCACTTTCTGCACATATAATCTCTACTTTCAATCGTATCCGTATTCTAGATGCAACAGTGTTTCAATTACCTGATCAA
TTCGCCACTGACTATCAAGGTTCAGGTGGGAGTAGTAATAAGGCAGGCGTGAAAATCCAATTGGAATATGATTTGCTTAGTGGTCAATTCCTTAACGTGC
AGCTTGGACCAGGAAAGAATAATGATAAAACATATGGTACCATCTGTCTTGAAACCGTAGAAGCAGGCGATTTGTGTTTACGTGATCTTGGTTACTTCGA
TTTAGGAGACTTACAAACCATTCATGATAAAGAGGCTTACTATATCTCACGGTTGAAGTTGAATACACGCATTTATATCAAGAACCCTGAACCCGAATAT
TTCAATAATGGTACGTTAAAAAAGCAAACAGAATACATCCAGCTTGATATGACACAAATGATGTCTGGTCTAATGCCCGGTGAAACGATGGAAATACCCG
AGGCATACATTGGCCAAAATCAGAAACTTCCAGCACGTGTCATTATCCATCGTTTAACCGATGAACAAACACAAACACGTCTGAAAAACCAAGCGATACG
CGAAAAGAAGAAAGGCATTGTCATGAAGGATAAAAGTAAACGTTTAATGGGGATGAATGTATATATCACGAATACGTCGTTCGAAGAAGTACCGACGAAC
TACGTGCATTCATTATATTCACTTCGTTGGCAGATTGAAATTTTGTTTAAAACGTGGAAGTCATTCTTTGAAATTGATGAATGTCAAAATATAAAAAGAG
AACGCCTAGAGTGCCATTTATATGGACAACTCATCGGCATTCTCCTCTGTTCTTCTACGATGTTCCAAATGCGTCAGTTTCTGCTTGAAAAGAAAAAGCA
GGAGCTGAGTGAATACAAAGCGATTTATATGATTAAAGATTACTTTCCGTTACTTTTTCAAGCGATAGCTGTTGGCACAGAAGAACTTTTAAAAGTTCTG
CATCGCCTTTATCAACTGCTCAAAAAGAATGGTCGTAAATGTCATAGACATAAGAAGATGACCGTCTTTGATATTCTAGGGATTGTGTATAAAACGACGG
TGAAGCATAGACAAACTGCCTAGCGAAAAAATCGCGTTTTAATAGGCTTATTTAGCATGCCTACTTTTTTATCACTTGCTAGTTTTGAAACAAGGATCAT
TTCGTACCTAGTAAATTACTTGGTTAGCTTGATGGGCATG
TTTCTTTAACGGCATGACGATTTTATCTTATTCAGCCTTATTTAAATTTACTCGCAGGACAAATAAACCCTACATCTTTTGCGAACCTTTGAAGAATCTG
AGGGGATAAATGATGATGTAATTCCTGAGAGAATAGTTGAAACCCTTTTGCTAGTGAAATATCCATAAAAACACCATCCTTTTCATTGACCCTACTAAAG
GGAGAGTGTAAATGTTCTTTAGGAAATATATACTTAAGTAAATTAGCACTTATATTTTATTTGATAGTTATAGAAAAGCAAAAATAAAGATAAATTCTAC
ATTAAAAGAAGGATATTATTTTTTGTGTCGAATCAACTTATATAAGTATATAGTATTAGGTGACATGGGACAGTAGGAACCCATGTTGGCGTCATTTGTT
AAATGTAATTCCTTTTGCAGCGCAGTGCCTCCTTATACAAGGGGGCTTTTTTGTTGTAAATACATAATATATGGAGGCATAAGAGAGATGACAGACGAGT
ATAAACACTTAATTTCGTATGTAGATAAAATAAAAGAGCTTAATTATAGTACAGCTTCGGCATTAATAGTTATTCATAATGATAAAATTGTAGTAAACCA
TTATAACGGAAGGCATTCAAATGTTGCTTCATCAAAAAAGATCTCGCAATCATCACAATTCCATGTTGCTTCTGCACGTAAAAGTTACCTGGGTCTAGCG
GTTGCTTATGCCCTCTATGAGAGGAAAATTAAAAGTTTAGATGATTACGCAAGAGATTATTTTGATGAATATGATTGGGATGTTCTTGGGGATACCACTT
TAAGACATTTAGTGACATATTCTCATGGAATTGATGACAATGATGGAGCAATTTTTAGAGAATTTGGACCAGGAGAGCGTTGGGCGTATAAAGGAATGGG
GGTCATCATGATGACGAAACTCATTAACAACTTATACAAAAAGCCTTTTACACAATTACTTAAAGAAAGAGTTTTTGAGCCTCTTAATTTCAAAGAGACT
GCTTGGAGGACAATAGAGATCGATGAGCTTGTTAAAGTAATTACCGATCCCAGTAGGGATGCGATACTACCAATCAATCATTCAACAAGTGGGTTGGAAG
GGAATCTCTTTGTTTCTACAAGAGAGTTTGCATATTGGGGATATCTTCATTTAAAGAAAGGATTCATGAATGGAAAGCAAATAGTTCCTAAAGAAGTGAT
TGAAATTGCGACAAAAGTACAAAGTCCAATCTATAAAGACAGAACGCTCCCTGAAAATGGCCTATTTTGGTATGTCCAAAATGGCCATACTTTAAAAAGT
GAAATGGGTGAAAGGGTACCCGTAGGTTCATTCCAAATACTTGGAAACACAGGCCCGACTATCCTGGTAATTCCTAAATATAACGTGGTTGTAGCAAAAA
TGTATAATAAAAAATACAATTATGGAGGGGATAATTATCTTCATTACTTAAGAGAATTTAGCAATTTAGTTGCGGATACTTTTGCAAAATAATCTCATTT
ATTCAATTACTACTTAAAAACACAAAAACGTAATTACTTATTGTTTTACTGTTTTGGAGATACTTCAAAATTTAAGATTGATGGCTATTGGGTCACCATA
CATCATTATCAACCTCAGATAAATTTATATCCATAATTCACAGAAAACGTTATTCTTTTTGTAGAAATATACAGAAAGGATGGCGTTTTTGTATGAATCT
ATCGATTCAGGATGAGTTTCATTTGTTCGCTGAAGAGTTACAGCGATATCTATCTCCACATATTCTTCAACAACTCGCACAAGAGACAGGTTTTGTAAAA
CGTAAAAGTAAGTATGGCGCACGAGATTTGGCCGCTTTATGTATTTGGATTAGTCAACACGTAGCAAGCGATTCTCTTACTCGATTATGTAGTCAGCTTT
ATGCAAATACAGCTACACTTATGAGTCCAGAAGGACTGAATCAACGCTTTAATCCAGCAGCTGTCACATTTCTTCGAGAAGTCTTTACTTCTCTTCTCAC
ACAAAAACTCTGTTCAAATCACTCACTTTCTGCACATATAATCTCTACTTTCAATCGTATCCGTATTCTAGATGCAACAGTGTTTCAATTACCTGATCAA
TTCGCCACTGACTATCAAGGTTCAGGTGGGAGTAGTAATAAGGCAGGCGTGAAAATCCAATTGGAATATGATTTGCTTAGTGGTCAATTCCTTAACGTGC
AGCTTGGACCAGGAAAGAATAATGATAAAACATATGGTACCATCTGTCTTGAAACCGTAGAAGCAGGCGATTTGTGTTTACGTGATCTTGGTTACTTCGA
TTTAGGAGACTTACAAACCATTCATGATAAAGAGGCTTACTATATCTCACGGTTGAAGTTGAATACACGCATTTATATCAAGAACCCTGAACCCGAATAT
TTCAATAATGGTACGTTAAAAAAGCAAACAGAATACATCCAGCTTGATATGACACAAATGATGTCTGGTCTAATGCCCGGTGAAACGATGGAAATACCCG
AGGCATACATTGGCCAAAATCAGAAACTTCCAGCACGTGTCATTATCCATCGTTTAACCGATGAACAAACACAAACACGTCTGAAAAACCAAGCGATACG
CGAAAAGAAGAAAGGCATTGTCATGAAGGATAAAAGTAAACGTTTAATGGGGATGAATGTATATATCACGAATACGTCGTTCGAAGAAGTACCGACGAAC
TACGTGCATTCATTATATTCACTTCGTTGGCAGATTGAAATTTTGTTTAAAACGTGGAAGTCATTCTTTGAAATTGATGAATGTCAAAATATAAAAAGAG
AACGCCTAGAGTGCCATTTATATGGACAACTCATCGGCATTCTCCTCTGTTCTTCTACGATGTTCCAAATGCGTCAGTTTCTGCTTGAAAAGAAAAAGCA
GGAGCTGAGTGAATACAAAGCGATTTATATGATTAAAGATTACTTTCCGTTACTTTTTCAAGCGATAGCTGTTGGCACAGAAGAACTTTTAAAAGTTCTG
CATCGCCTTTATCAACTGCTCAAAAAGAATGGTCGTAAATGTCATAGACATAAGAAGATGACCGTCTTTGATATTCTAGGGATTGTGTATAAAACGACGG
TGAAGCATAGACAAACTGCCTAGCGAAAAAATCGCGTTTTAATAGGCTTATTTAGCATGCCTACTTTTTTATCACTTGCTAGTTTTGAAACAAGGATCAT
TTCGTACCTAGTAAATTACTTGGTTAGCTTGATGGGCATG
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1428 bp | 476 aa | 1793 | 3220 | + | No |
Chemistry : DDE
ORF sequence :
MNLSIQDEFHLFAEELQRYLSPHILQQLAQETGFVKRKSKYGARDLAALCIWISQHVASDSLTRLCSQLYANTATLMSPEGLNQRFNPAAVTFLREVFTS
LLTQKLCSNHSLSAHIISTFNRIRILDATVFQLPDQFATDYQGSGGSSNKAGVKIQLEYDLLSGQFLNVQLGPGKNNDKTYGTICLETVEAGDLCLRDLG
YFDLGDLQTIHDKEAYYISRLKLNTRIYIKNPEPEYFNNGTLKKQTEYIQLDMTQMMSGLMPGETMEIPEAYIGQNQKLPARVIIHRLTDEQTQTRLKNQ
AIREKKKGIVMKDKSKRLMGMNVYITNTSFEEVPTNYVHSLYSLRWQIEILFKTWKSFFEIDECQNIKRERLECHLYGQLIGILLCSSTMFQMRQFLLEK
KKQELSEYKAIYMIKDYFPLLFQAIAVGTEELLKVLHRLYQLLKKNGRKCHRHKKMTVFDILGIVYKTTVKHRQTA
LLTQKLCSNHSLSAHIISTFNRIRILDATVFQLPDQFATDYQGSGGSSNKAGVKIQLEYDLLSGQFLNVQLGPGKNNDKTYGTICLETVEAGDLCLRDLG
YFDLGDLQTIHDKEAYYISRLKLNTRIYIKNPEPEYFNNGTLKKQTEYIQLDMTQMMSGLMPGETMEIPEAYIGQNQKLPARVIIHRLTDEQTQTRLKNQ
AIREKKKGIVMKDKSKRLMGMNVYITNTSFEEVPTNYVHSLYSLRWQIEILFKTWKSFFEIDECQNIKRERLECHLYGQLIGILLCSSTMFQMRQFLLEK
KKQELSEYKAIYMIKDYFPLLFQAIAVGTEELLKVLHRLYQLLKKNGRKCHRHKKMTVFDILGIVYKTTVKHRQTA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1107 bp | 369 aa | 483 | 1589 | + | No |
Description :
ORF sequence :
Blast result :
Comments
ISBce4 is 95% aa similar to ISBth5. ISBce4 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). In addition to the transposase gene, ISBce4 harbors a beta-lactamase resistance determinant. The copy number of ISBce4 in Bacillus cereus E33L (plasmid pE33L466) is 1.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18