ISLsa2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CR936503 | ND | Lactobacillus sakei | Lactobacillus sakei 23K |
DNA section
IS Length : 1454 bp
Ends
IR Length : 22/24
IRL : TGAAGTGAACCCCCGATATTGGACCAGAATCCAATATCGGGGGTTTTTAT
IRR : TGAAGTGAACCCCAAATATTGGACGAAACTTTAAGCAGCTTTCTGGGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGGGCAAAATTACAT | TTG | TCCTAACGCCTTTT | 3 |
DNA sequence
TGAAGTGAACCCCCGATATTGGACCAGAATCCAATATCGGGGGTTTTTATATTGACCAAATATTCTAAAAAATTAAAAATAAGAGTTGTTCAAGATTATC
TGACTTCCCCAATAGGATACAGATTAATTGCTAAAAAATATGGTATTAAAAGTTCTTCATCAGTTCTTTTTTGGGTCCGACGTTATAAGACCTTTGGTCT
AAAGGGGCTCGATGTCTTATCATCTGAGAAAATATTTGATGGCTCATTTAAGGTGAATGTTTTAAAATGGACGAAAACGAATAAGGCTTCTTATCCCAAA
ACTGCACTTCATTTTAATATCTCAAACGTTGGTACTATCTGGCAATGGCAATATATTTTGGAGACTGAGGGTGCTGATGCCTTATATCGTTCCAAAGGAC
GGCAAATAATTATGTCAGCAGATAAACAATCAAAAAAACGGCAACAAACAGAACTAGAACGTCTTAGAGAAGAAAATGAATTATTACAAATCGAGAACGA
ATATCTAAAAAAATTAAGAGCCTTAGTCCAGGAAGAAGAAGACAACAAGCACAAGTCATCCAAGAGCTAAGGTTTAGACATAAATTAGTAAAAATTCTAA
AAGTAGTTGGTATGGCTAAAAGTACGTATGAATATATTATTAGTCATGAACCGAACGGTTCATCTGACCAATCTGTTAAACATGCCATCCAAACAATCAA
GAAGGATCATCCAGCTTATGGTTATCGCCGCGTAACTGGTGAATTGCATCGTATGAATATCTTAGTAAATCATAAAAAGGTGCTTGTCTTAATGCGCGAA
ATGCAATTACTATCTACCGCATTTAACAAACGTACTCGTAAGTATAATTCGTATAAAGGAAATGTTGGAACTGTTGCTAAGAACTTAATCAACCGTCGGT
TCTTCACTGATCGTCCTTATCAAAAACTAGCGACAGATGTTACTGAGGTTCGTTGGGGAGCCAAGACAATTGATGAACGTGCCTATTTCACATGTATTTA
TGACCTATTCTCAGGTGAAATTCTTAGCCATCAAGTTAGCAAACATCCTACTGTTGAATTCACAACAACTGTTTTGAATCGGGCGGTTAAGAAAATCCCT
AAGGATTTAGAATACCGGACAACAATTCACTCAGACCAAGGGTTTCAATACCAACATCAAAATTGGACTAATATTTTGAAAGAACATCGCATTTTTCAAA
GTATGTCTCGCAAAGCAACATGTTTAGATAATGCGTCAATGGAGAGTTTCTTTCATATTATGAAAGCTGAAGCTTTCTACCAAAAAGAAACCGATACTTA
TGAAACGTTAGTTTCACAAATATCGGTTTGGATTGATGATTATAATTTTTCAAGAATTAAAACAAAACTGGGTTGTAAAAGTCCGGTTGAATACCGAATT
TTTACAACCCAGAAAGCTGCTTAAAGTTTCGTCCAATATTTGGGGTTCACTTCA
TGACTTCCCCAATAGGATACAGATTAATTGCTAAAAAATATGGTATTAAAAGTTCTTCATCAGTTCTTTTTTGGGTCCGACGTTATAAGACCTTTGGTCT
AAAGGGGCTCGATGTCTTATCATCTGAGAAAATATTTGATGGCTCATTTAAGGTGAATGTTTTAAAATGGACGAAAACGAATAAGGCTTCTTATCCCAAA
ACTGCACTTCATTTTAATATCTCAAACGTTGGTACTATCTGGCAATGGCAATATATTTTGGAGACTGAGGGTGCTGATGCCTTATATCGTTCCAAAGGAC
GGCAAATAATTATGTCAGCAGATAAACAATCAAAAAAACGGCAACAAACAGAACTAGAACGTCTTAGAGAAGAAAATGAATTATTACAAATCGAGAACGA
ATATCTAAAAAAATTAAGAGCCTTAGTCCAGGAAGAAGAAGACAACAAGCACAAGTCATCCAAGAGCTAAGGTTTAGACATAAATTAGTAAAAATTCTAA
AAGTAGTTGGTATGGCTAAAAGTACGTATGAATATATTATTAGTCATGAACCGAACGGTTCATCTGACCAATCTGTTAAACATGCCATCCAAACAATCAA
GAAGGATCATCCAGCTTATGGTTATCGCCGCGTAACTGGTGAATTGCATCGTATGAATATCTTAGTAAATCATAAAAAGGTGCTTGTCTTAATGCGCGAA
ATGCAATTACTATCTACCGCATTTAACAAACGTACTCGTAAGTATAATTCGTATAAAGGAAATGTTGGAACTGTTGCTAAGAACTTAATCAACCGTCGGT
TCTTCACTGATCGTCCTTATCAAAAACTAGCGACAGATGTTACTGAGGTTCGTTGGGGAGCCAAGACAATTGATGAACGTGCCTATTTCACATGTATTTA
TGACCTATTCTCAGGTGAAATTCTTAGCCATCAAGTTAGCAAACATCCTACTGTTGAATTCACAACAACTGTTTTGAATCGGGCGGTTAAGAAAATCCCT
AAGGATTTAGAATACCGGACAACAATTCACTCAGACCAAGGGTTTCAATACCAACATCAAAATTGGACTAATATTTTGAAAGAACATCGCATTTTTCAAA
GTATGTCTCGCAAAGCAACATGTTTAGATAATGCGTCAATGGAGAGTTTCTTTCATATTATGAAAGCTGAAGCTTTCTACCAAAAAGAAACCGATACTTA
TGAAACGTTAGTTTCACAAATATCGGTTTGGATTGATGATTATAATTTTTCAAGAATTAAAACAAAACTGGGTTGTAAAAGTCCGGTTGAATACCGAATT
TTTACAACCCAGAAAGCTGCTTAAAGTTTCGTCCAATATTTGGGGTTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 519 bp | 172 aa | 52 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
LTKYSKKLKIRVVQDYLTSPIGYRLIAKKYGIKSSSSVLFWVRRYKTFGLKGLDVLSSEKIFDGSFKVNVLKWTKTNKASYPKTALHFNISNVGTIWQWQ
YILETEGADALYRSKGRQIIMSADKQSKKRQQTELERLREENELLQIENEYLKKLRALVQEEEDNKHKSSKS
YILETEGADALYRSKGRQIIMSADKQSKKRQQTELERLREENELLQIENEYLKKLRALVQEEEDNKHKSSKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 944 bp | 314 aa | 481 | 1424 | + | No |
Description : Second part of the transposase
ORF sequence :
IITNRERISKKIKSLSPGRRRQQAQVIQELRFRHKLVKILKVVGMAKSTYEYIISHEPNGSSDQSVKHAIQTIKKDHPAYGYRRVTGELHRMNILVNHKK
VLVLMREMQLLSTAFNKRTRKYNSYKGNVGTVAKNLINRRFFTDRPYQKLATDVTEVRWGAKTIDERAYFTCIYDLFSGEILSHQVSKHPTVEFTTTVLN
RAVKKIPKDLEYRTTIHSDQGFQYQHQNWTNILKEHRIFQSMSRKATCLDNASMESFFHIMKAEAFYQKETDTYETLVSQISVWIDDYNFSRIKTKLGCK
SPVEYRIFTTQKAA
VLVLMREMQLLSTAFNKRTRKYNSYKGNVGTVAKNLINRRFFTDRPYQKLATDVTEVRWGAKTIDERAYFTCIYDLFSGEILSHQVSKHPTVEFTTTVLN
RAVKKIPKDLEYRTTIHSDQGFQYQHQNWTNILKEHRIFQSMSRKATCLDNASMESFFHIMKAEAFYQKETDTYETLVSQISVWIDDYNFSRIKTKLGCK
SPVEYRIFTTQKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1373 bp | 457 aa | 52 | 1424 | + | Yes |
Chemistry : DDE
ORF sequence :
LTKYSKKLKIRVVQDYLTSPIGYRLIAKKYGIKSSSSVLFWVRRYKTFGLKGLDVLSSEKIFDGSFKVNVLKWTKTNKASYPKTALHFNISNVGTIWQWQ
YILETEGADALYRSKGRQIIMSADKQSKKRQQTELERLREENELLQIENEYLKKIKSLSPGRRRQQAQVIQELRFRHKLVKILKVVGMAKSTYEYIISHE
PNGSSDQSVKHAIQTIKKDHPAYGYRRVTGELHRMNILVNHKKVLVLMREMQLLSTAFNKRTRKYNSYKGNVGTVAKNLINRRFFTDRPYQKLATDVTEV
RWGAKTIDERAYFTCIYDLFSGEILSHQVSKHPTVEFTTTVLNRAVKKIPKDLEYRTTIHSDQGFQYQHQNWTNILKEHRIFQSMSRKATCLDNASMESF
FHIMKAEAFYQKETDTYETLVSQISVWIDDYNFSRIKTKLGCKSPVEYRIFTTQKAA
YILETEGADALYRSKGRQIIMSADKQSKKRQQTELERLREENELLQIENEYLKKIKSLSPGRRRQQAQVIQELRFRHKLVKILKVVGMAKSTYEYIISHE
PNGSSDQSVKHAIQTIKKDHPAYGYRRVTGELHRMNILVNHKKVLVLMREMQLLSTAFNKRTRKYNSYKGNVGTVAKNLINRRFFTDRPYQKLATDVTEV
RWGAKTIDERAYFTCIYDLFSGEILSHQVSKHPTVEFTTTVLNRAVKKIPKDLEYRTTIHSDQGFQYQHQNWTNILKEHRIFQSMSRKATCLDNASMESF
FHIMKAEAFYQKETDTYETLVSQISVWIDDYNFSRIKTKLGCKSPVEYRIFTTQKAA
Blast result :
Comments
ISLsa2 is 56%(ORFA) and 57% (ORFB) aa similar to ISEnfa3. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chaillou,S., Champomier-Verges,M., Cornet,M., Crutz Lecoq,A.-M., Dudez,A.-M., Martin,V., Beaufils,S., Darbon-Rongere,E., Bossy,R., Loux,V. and Zagorec,M.
(2005) Nat. Biotechnol. 23 (12), 1494-1495
(2005) Nat. Biotechnol. 23 (12), 1494-1495