ISHne1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008358 | ND | Hyphomonas neptunium | Hyphomonas neptunium ATCC 15444 |
DNA section
IS Length : 1324 bp
Ends
IR Length : 28/39
IRL : TGAACCGTGCCGGGTTTGCCGGAGGCCATTTTCTTTGAGAGAGTGGCCCA
IRR : TGAACCGCGCCGGTAACCCCGGAGGCTGTTTGGTTGGAGTCATGCAGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATTGAATTGGATGGAC | AGC | AAGCGCAACCGGA | 3 |
DNA sequence
TGAACCGTGCCGGGTTTGCCGGAGGCCATTTTCTTTGAGAGAGTGGCCCAATGACAGACAAGAAGCAGAGACCTCATTACTCGCCAGAGGTGCGTGATCG
AGCGGTTCGGATGGTGATGGAGCATGGCGGGGATTACCCGTCGCAGTGGGCAGCGATCGTTTCGATTGCCGGCAAGATCGTGTGCGTGCCGCAGACACTG
CACGGCTGGGTGGCGCAGGCTGAGAGGGACGTGGGCAAGCGGTCCGGGCCCAGCACCGAGGAACGCGAACGGATCAAGGCGCTTGAACGCGAGAACCGGG
AGCTTCGCCAGGCGAACGAGATCCTCCGGAAGGCATCAGCGTATTTTGCTCAGGCGGAGCTCGACCGCCGACCGAAGACATGATCGCGTTCATTGACGAC
CATCGCGATGTTTATGGGGTCGAGCCGATCTGCAGGGTATTGCCGATTGCCCCATCAACCTTTCATGAGGCGGATGCGCGGCGGCGGCGCCCCGACCGCA
GGCCGCCGCGCATCCGCCGGGACGAGACGCTGAAGCCGGAGATCCTTCGGGTCTTCAACGAGAACTTCACCGTCTATGGCGCTAACAAGGTCTGGCACCA
GATGAAGCGGGAAGGCTTTGACGTGGCCCGCTGCACCGTCAGGCGCCTGATGAAGGCCATGGGTCTGAAGGGCATTATCCGCGGCAAGCCGCATCGCACG
ACCTACAGCGACAAGGTAGCGCCGTGCCCGCTCGACCATGTGAAGCGCAACTTCAAGGCTCCGGCGCCGAACCGGCTCTGGGTCAGCGATTTCACCTACG
TCTCGACCTGGGCTGGATTTGTCTACGTCGCCTTCGTCATTGATGTGTTCGCCCGCCGCATTGTCGGCTGGCGGGTCAGCCGGACAGCGCATGCGCGCTT
TGTACTCGATGCTCTTGAGCAGGCCCTGCATGACCGGCGGCCGGGCATCGGCAGCGGACTTGTTCATCACTCGGATCGTGGCAGCCAGTATGTCAGCATA
CGGTATACGGAGCGTCTGGCGGAGGCAGGCCTCGAGCCGTCGGTCGGCAGTGTCGGCGATAGTTATGACAACGCCCTCGCCGAGACGATCAATGGACTCT
ACAAGGCCGAAGTCATCTGGCGGAAAGGCCCTTGGAAATCCATGGACGCCGTCGAATACGCCACGCTCGAGTGGGTCGACTGGTTCAATCACCGCCGCCT
GCTCGAGCCGATCGGAAACATCCCTCCGGCAGAAGCCGAGGCCAATTACTTTGCAGCCCTAAAGGAAAACGCTACCGCTGCATGACTCCAACCAAACAGC
CTCCGGGGTTACCGGCGCGGTTCA
AGCGGTTCGGATGGTGATGGAGCATGGCGGGGATTACCCGTCGCAGTGGGCAGCGATCGTTTCGATTGCCGGCAAGATCGTGTGCGTGCCGCAGACACTG
CACGGCTGGGTGGCGCAGGCTGAGAGGGACGTGGGCAAGCGGTCCGGGCCCAGCACCGAGGAACGCGAACGGATCAAGGCGCTTGAACGCGAGAACCGGG
AGCTTCGCCAGGCGAACGAGATCCTCCGGAAGGCATCAGCGTATTTTGCTCAGGCGGAGCTCGACCGCCGACCGAAGACATGATCGCGTTCATTGACGAC
CATCGCGATGTTTATGGGGTCGAGCCGATCTGCAGGGTATTGCCGATTGCCCCATCAACCTTTCATGAGGCGGATGCGCGGCGGCGGCGCCCCGACCGCA
GGCCGCCGCGCATCCGCCGGGACGAGACGCTGAAGCCGGAGATCCTTCGGGTCTTCAACGAGAACTTCACCGTCTATGGCGCTAACAAGGTCTGGCACCA
GATGAAGCGGGAAGGCTTTGACGTGGCCCGCTGCACCGTCAGGCGCCTGATGAAGGCCATGGGTCTGAAGGGCATTATCCGCGGCAAGCCGCATCGCACG
ACCTACAGCGACAAGGTAGCGCCGTGCCCGCTCGACCATGTGAAGCGCAACTTCAAGGCTCCGGCGCCGAACCGGCTCTGGGTCAGCGATTTCACCTACG
TCTCGACCTGGGCTGGATTTGTCTACGTCGCCTTCGTCATTGATGTGTTCGCCCGCCGCATTGTCGGCTGGCGGGTCAGCCGGACAGCGCATGCGCGCTT
TGTACTCGATGCTCTTGAGCAGGCCCTGCATGACCGGCGGCCGGGCATCGGCAGCGGACTTGTTCATCACTCGGATCGTGGCAGCCAGTATGTCAGCATA
CGGTATACGGAGCGTCTGGCGGAGGCAGGCCTCGAGCCGTCGGTCGGCAGTGTCGGCGATAGTTATGACAACGCCCTCGCCGAGACGATCAATGGACTCT
ACAAGGCCGAAGTCATCTGGCGGAAAGGCCCTTGGAAATCCATGGACGCCGTCGAATACGCCACGCTCGAGTGGGTCGACTGGTTCAATCACCGCCGCCT
GCTCGAGCCGATCGGAAACATCCCTCCGGCAGAAGCCGAGGCCAATTACTTTGCAGCCCTAAAGGAAAACGCTACCGCTGCATGACTCCAACCAAACAGC
CTCCGGGGTTACCGGCGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 333 bp | 110 aa | 51 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MTDKKQRPHYSPEVRDRAVRMVMEHGGDYPSQWAAIVSIAGKIVCVPQTLHGWVAQAERDVGKRSGPSTEERERIKALERENRELRQANEILRKASAYFA
QAELDRRPKT
QAELDRRPKT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 380 | 1285 | + | No |
Description : Second part of the transposase
ORF sequence :
MIAFIDDHRDVYGVEPICRVLPIAPSTFHEADARRRRPDRRPPRIRRDETLKPEILRVFNENFTVYGANKVWHQMKREGFDVARCTVRRLMKAMGLKGII
RGKPHRTTYSDKVAPCPLDHVKRNFKAPAPNRLWVSDFTYVSTWAGFVYVAFVIDVFARRIVGWRVSRTAHARFVLDALEQALHDRRPGIGSGLVHHSDR
GSQYVSIRYTERLAEAGLEPSVGSVGDSYDNALAETINGLYKAEVIWRKGPWKSMDAVEYATLEWVDWFNHRRLLEPIGNIPPAEAEANYFAALKENATA
A
RGKPHRTTYSDKVAPCPLDHVKRNFKAPAPNRLWVSDFTYVSTWAGFVYVAFVIDVFARRIVGWRVSRTAHARFVLDALEQALHDRRPGIGSGLVHHSDR
GSQYVSIRYTERLAEAGLEPSVGSVGDSYDNALAETINGLYKAEVIWRKGPWKSMDAVEYATLEWVDWFNHRRLLEPIGNIPPAEAEANYFAALKENATA
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1235 bp | 411 aa | 51 | 1285 | + | Yes |
Chemistry : DDE
ORF sequence :
MTDKKQRPHYSPEVRDRAVRMVMEHGGDYPSQWAAIVSIAGKIVCVPQTLHGWVAQAERDVGKRSGPSTEERERMIAFIDDHRDVYGVEPICRVLPIAPS
TFHEADARRRRPDRRPPRIRRDETLKPEILRVFNENFTVYGANKVWHQMKREGFDVARCTVRRLMKAMGLKGIIRGKPHRTTYSDKVAPCPLDHVKRNFK
APAPNRLWVSDFTYVSTWAGFVYVAFVIDVFARRIVGWRVSRTAHARFVLDALEQALHDRRPGIGSGLVHHSDRGSQYVSIRYTERLAEAGLEPSVGSVG
DSYDNALAETINGLYKAEVIWRKGPWKSMDAVEYATLEWVDWFNHRRLLEPIGNIPPAEAEANYFAALKENATAAIKALERENRELRQANEILRKASAYF
CSGGARPPTED
TFHEADARRRRPDRRPPRIRRDETLKPEILRVFNENFTVYGANKVWHQMKREGFDVARCTVRRLMKAMGLKGIIRGKPHRTTYSDKVAPCPLDHVKRNFK
APAPNRLWVSDFTYVSTWAGFVYVAFVIDVFARRIVGWRVSRTAHARFVLDALEQALHDRRPGIGSGLVHHSDRGSQYVSIRYTERLAEAGLEPSVGSVG
DSYDNALAETINGLYKAEVIWRKGPWKSMDAVEYATLEWVDWFNHRRLLEPIGNIPPAEAEANYFAALKENATAAIKALERENRELRQANEILRKASAYF
CSGGARPPTED
Blast result :
Comments
ISHne1 is 82%(ORFA) and 84%(ORFB) aa similar to ISRtr2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible-1 frameshift.
References
1] Badger,J.H., Hoover,T.R., Brun,Y.V., Weiner,R.M., Laub,M.T., Alexandre,G., Mrazek,J., Ren,Q., Paulsen,I.T., Nelson,K.E., Khouri,H.M., Radune,D., Sosa,J., Dodson,R.J., Sullivan,S.A., Rosovitz,M.J., Madupu,R., Brinkac,L.M., Durkin,A.S., Daugherty,S.C., Kothari,S.P., Giglio,M.G., Zhou,L., Haft,D.H., Selengut,J.D., Davidsen,T.M., Yang,Q., Zafar,N. and Ward,N.L. (2006) J. Bacteriol. 188 (19), 6841-6850