ISSty2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003197 | ND | Salmonella typhimurium | Salmonella typhimurium LT2 |
DNA section
IS Length : 1259 bp
Ends
IR Length : 30/41
IRL : TGATCTTACCCAGCAATAGTGGACACGCGACTAAGTGAGTAAACTCTCAA
IRR : TGATCCTACCCGCGTAATATGGGCACAACCCTAAGCGAGGTTCTGGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAACCTACC | AGCTTCCTTC | 0 |
DNA sequence
TGATCTTACCCAGCAATAGTGGACACGCGACTAAGTGAGTAAACTCTCAAGTAAGAGGTGACTCACATGACAAAACCAGCATTAACCACCAAAAAGCCAC
GCAAGCAGCACACGCCTGAATTTCGTCAGGAAGCCCTGAAACTGGCTAAGCGCATTGGTGTTGCCGCTGCAGCCCGTGAGCTCAGCCTGTATAAATCACA
GCTCCACAACTGGCGAAGCAAACAGCAAAATCAGCTCTCTTCTTCTGAACGCGAGCAGGAGATGTCCGCTGAGATCGCCCGTCTGAAACGTCAACTGGCA
GAACGGGATGAGGAACTGGCCATTCTCCAGAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTCATCGAAAAACATCAGGCTGAGTT
CAATATCAAAGCCATGTGCCGTGTATTTCAGGTTGCCCGTAGCGGCTGGTACGTCTGGCATCAGCGTCGTCATCAGATAAACCGGCGTCAGCGGTTCCGC
CTTGTCTGTGATAACGTCGTCCGGGAAGCATTCAGTGACGCAAAACAACGCTATGGTGCGCCACGCCTGACGGATGAGCTTCGTGCTCAGGGTTACCAGT
TCAACGTGAAAACCGTGGCGGCCAGCCTGCGTCGGCAGGGGCTGCGGGCGAAAGCCTCGCGCCGGTTCCGTCCGGTCAGTTACCGCAAACATGGTCTGCC
AGTCTCAGAGAATCTGCTGAAGCAGGACTTTTACGCCAGCGGCCCGAATCAGAAGTGGGTGGGTGACATCACGTATCTTCGCACTGGTGAAGGCTGGCTT
TATCTGGCCGTGGTTATCGATCTGTGGCCGCGGTCAGTCATTGGCTGGTCGATGTCCTCGCGGATGACAGCACAGCTTGCCTGCAATGCGTTACAGATGG
CGCTGTGGCAGCGTAAATGTCCGGAAAATGTCATCGTGCATACAGACAGAGGCGGTCAGTACTGTTCAACGGATTACCAGAGCTTACTGAAACGCCATAA
TCTGCGGGGAAGTATGAGCGCCAGAGGTTGTTGTTACGACAATGCCTGTGCGGAAAGCTTCTTCCATACGCTGAGGGTGGAATGTATCCACGGAGAGGAC
TTTGTCAGCCGGGAAATAATGCGGACAGCAGTGTTTAATTATAGTGAGTGTGATTACAATCGGTGGCGTCGTCACAGTGCCTGTGGCGGTCTCAGTCCGG
AACAATTTGAAAACCAGAACCTCGCTTAGGGTTGTGCCCATATTACGCGGGTAGGATCA
GCAAGCAGCACACGCCTGAATTTCGTCAGGAAGCCCTGAAACTGGCTAAGCGCATTGGTGTTGCCGCTGCAGCCCGTGAGCTCAGCCTGTATAAATCACA
GCTCCACAACTGGCGAAGCAAACAGCAAAATCAGCTCTCTTCTTCTGAACGCGAGCAGGAGATGTCCGCTGAGATCGCCCGTCTGAAACGTCAACTGGCA
GAACGGGATGAGGAACTGGCCATTCTCCAGAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTCATCGAAAAACATCAGGCTGAGTT
CAATATCAAAGCCATGTGCCGTGTATTTCAGGTTGCCCGTAGCGGCTGGTACGTCTGGCATCAGCGTCGTCATCAGATAAACCGGCGTCAGCGGTTCCGC
CTTGTCTGTGATAACGTCGTCCGGGAAGCATTCAGTGACGCAAAACAACGCTATGGTGCGCCACGCCTGACGGATGAGCTTCGTGCTCAGGGTTACCAGT
TCAACGTGAAAACCGTGGCGGCCAGCCTGCGTCGGCAGGGGCTGCGGGCGAAAGCCTCGCGCCGGTTCCGTCCGGTCAGTTACCGCAAACATGGTCTGCC
AGTCTCAGAGAATCTGCTGAAGCAGGACTTTTACGCCAGCGGCCCGAATCAGAAGTGGGTGGGTGACATCACGTATCTTCGCACTGGTGAAGGCTGGCTT
TATCTGGCCGTGGTTATCGATCTGTGGCCGCGGTCAGTCATTGGCTGGTCGATGTCCTCGCGGATGACAGCACAGCTTGCCTGCAATGCGTTACAGATGG
CGCTGTGGCAGCGTAAATGTCCGGAAAATGTCATCGTGCATACAGACAGAGGCGGTCAGTACTGTTCAACGGATTACCAGAGCTTACTGAAACGCCATAA
TCTGCGGGGAAGTATGAGCGCCAGAGGTTGTTGTTACGACAATGCCTGTGCGGAAAGCTTCTTCCATACGCTGAGGGTGGAATGTATCCACGGAGAGGAC
TTTGTCAGCCGGGAAATAATGCGGACAGCAGTGTTTAATTATAGTGAGTGTGATTACAATCGGTGGCGTCGTCACAGTGCCTGTGGCGGTCTCAGTCCGG
AACAATTTGAAAACCAGAACCTCGCTTAGGGTTGTGCCCATATTACGCGGGTAGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 67 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MTKPALTTKKPRKQHTPEFRQEALKLAKRIGVAAAARELSLYKSQLHNWRSKQQNQLSSSEREQEMSAEIARLKRQLAERDEELAILQKAATYFAKRLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 312 | 1229 | + | No |
Description : Second part of the transposase
ORF sequence :
GTGHSPEGRDILREAPEMKYVFIEKHQAEFNIKAMCRVFQVARSGWYVWHQRRHQINRRQRFRLVCDNVVREAFSDAKQRYGAPRLTDELRAQGYQFNVK
TVAASLRRQGLRAKASRRFRPVSYRKHGLPVSENLLKQDFYASGPNQKWVGDITYLRTGEGWLYLAVVIDLWPRSVIGWSMSSRMTAQLACNALQMALWQ
RKCPENVIVHTDRGGQYCSTDYQSLLKRHNLRGSMSARGCCYDNACAESFFHTLRVECIHGEDFVSREIMRTAVFNYSECDYNRWRRHSACGGLSPEQFE
NQNLA
TVAASLRRQGLRAKASRRFRPVSYRKHGLPVSENLLKQDFYASGPNQKWVGDITYLRTGEGWLYLAVVIDLWPRSVIGWSMSSRMTAQLACNALQMALWQ
RKCPENVIVHTDRGGQYCSTDYQSLLKRHNLRGSMSARGCCYDNACAESFFHTLRVECIHGEDFVSREIMRTAVFNYSECDYNRWRRHSACGGLSPEQFE
NQNLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 386 aa | 67 | 1229 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKPALTTKKPRKQHTPEFRQEALKLAKRIGVAAAARELSLYKSQLHNWRSKQQNQLSSSEREQEMSAEIARLKRQLAERDEELAILQKAATYFAKRLKK
YVFIEKHQAEFNIKAMCRVFQVARSGWYVWHQRRHQINRRQRFRLVCDNVVREAFSDAKQRYGAPRLTDELRAQGYQFNVKTVAASLRRQGLRAKASRRF
RPVSYRKHGLPVSENLLKQDFYASGPNQKWVGDITYLRTGEGWLYLAVVIDLWPRSVIGWSMSSRMTAQLACNALQMALWQRKCPENVIVHTDRGGQYCS
TDYQSLLKRHNLRGSMSARGCCYDNACAESFFHTLRVECIHGEDFVSREIMRTAVFNYSECDYNRWRRHSACGGLSPEQFENQNLA
YVFIEKHQAEFNIKAMCRVFQVARSGWYVWHQRRHQINRRQRFRLVCDNVVREAFSDAKQRYGAPRLTDELRAQGYQFNVKTVAASLRRQGLRAKASRRF
RPVSYRKHGLPVSENLLKQDFYASGPNQKWVGDITYLRTGEGWLYLAVVIDLWPRSVIGWSMSSRMTAQLACNALQMALWQRKCPENVIVHTDRGGQYCS
TDYQSLLKRHNLRGSMSARGCCYDNACAESFFHTLRVECIHGEDFVSREIMRTAVFNYSECDYNRWRRHSACGGLSPEQFENQNLA
Blast result :
Comments
ISSty2 is 98% (ORFA) and 96% (ORFB) aa similar to IS1230B . The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W., Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F., Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A., Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W., Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K. (2001) Nature 413 (6858), 852-856.