ISMxa1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008095 | ND | Myxococcus xanthus | Myxococcus xanthus DK |
DNA section
IS Length : 1292 bp
Ends
IR Length : 26/37
IRL : TGAAGTTGCCCCGGTTTCGTGGAGTTCCACCCGCTGTGCGAAAAGGGTCC
IRR : TGAAGTTGCTCCGGTTCCGCAGAGCGTTCCGCGCTTTCGCCCTCAAGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAACCCAAAG | GAC | CTGGACAATG | 3 |
DNA sequence
TGAAGTTGCCCCGGTTTCGTGGAGTTCCACCCGCTGTGCGAAAAGGGTCCCACGATGCCGAAGTCACGTCCCCCATACCCGCCGGAGTTCCGTGAGCGGG
TCGTCGAGCTGTTGAAAGCCGGCCGCTCCGGAAAGAGCCTCGCACGAGAGTTCGGCTGCACCGAGCAGAGCATCCGCAACTGGAAGAAGCAGTTGGAGGG
TAGTGGCCGTAGCGCTCCCAGCAGCAACGGCGAGAGCAGGCCAGCTCCGAGGGCGAGCAGTGCCGCCTCGGACGAGCGCGCCGAACTCGAGCGGCTGCGG
CGCGAGGTGCGGGTGCTGCGTGAGGAGCGTGACATCCTAAAAAAAGCTGCGGCCTGGTTCGCGCAGGAATCCGTGGCGACGCCCAAGCGGCGTACCGACT
CGTGAAGGCGAACCAGGCCGTCCACAGCATCACGACGCTCTGTCGTGTCCTGGAGGTGTCCGAGAGCGGCTACCACGCATGGCGGACGCGCCTGCCCTCG
AAGCGCGCGGTCGCGGACGCGGGCCTCAGCAAGCGCATTCGCGCCATCCACGCGATGAGCGACGGCACCTACGGCGTGCCGCGCATCCACGCCGAACTCG
CGGCAGAGGGCGTGCACATCGGCCGCAAGCGTGTCGCCCGTCTCATGCGGGCAGCGCACCTTGAGGGCGTCAGCCGCCGCAGGTGGTGCGTCACCACGAC
GCGCGACGAGCGCGCACGCCCTGCGCCCGACCTGGTCGACCGCAACTTCGCGGCCCCTGGACCCGACCACCTCTGGGTGGCTGACATCACGTACATTCCG
ACGCACGCCGGTTTCCTCTACCTCGCCGTCGTGCTTGACGCATGGAGCCGTAAAGTCGTCGGTTGGGCCATGGCTGGCCACCTCAAGACGGAGCTCGTCG
TGCAGGCACTCGACATGGCTGTGGGGCAGCGTCAGCCCCAAAGCGTCGTGCACCACAGCGACCAAGGGACGCAGTACACCTCGATTGGCTTCGGGCTTCG
GTGCAAGGAAGCCGGCGTGCGCCCATCCATGGGCAGCGTCGGCGACGCGTACGACAACGCGATGTGTGAAAGCTTCTTCGCCACGCTCGAGTGCGAACTG
CTCGCACGCCGCCGCTTCACGTCTCACGCCGAGGCGCGGATGGCCGTCTTCCGCTTCATCGAGGGCTGGTACAATCCGCTCCGGCGCCACTCCGCGCTCG
ACTACGAGTCACCCGCTGGTTACGAGAAGAAGCACCGCGCTGCCGCTTGAGGGCGAAAGCGCGGAACGCTCTGCGGAACCGGAGCAACTTCA
TCGTCGAGCTGTTGAAAGCCGGCCGCTCCGGAAAGAGCCTCGCACGAGAGTTCGGCTGCACCGAGCAGAGCATCCGCAACTGGAAGAAGCAGTTGGAGGG
TAGTGGCCGTAGCGCTCCCAGCAGCAACGGCGAGAGCAGGCCAGCTCCGAGGGCGAGCAGTGCCGCCTCGGACGAGCGCGCCGAACTCGAGCGGCTGCGG
CGCGAGGTGCGGGTGCTGCGTGAGGAGCGTGACATCCTAAAAAAAGCTGCGGCCTGGTTCGCGCAGGAATCCGTGGCGACGCCCAAGCGGCGTACCGACT
CGTGAAGGCGAACCAGGCCGTCCACAGCATCACGACGCTCTGTCGTGTCCTGGAGGTGTCCGAGAGCGGCTACCACGCATGGCGGACGCGCCTGCCCTCG
AAGCGCGCGGTCGCGGACGCGGGCCTCAGCAAGCGCATTCGCGCCATCCACGCGATGAGCGACGGCACCTACGGCGTGCCGCGCATCCACGCCGAACTCG
CGGCAGAGGGCGTGCACATCGGCCGCAAGCGTGTCGCCCGTCTCATGCGGGCAGCGCACCTTGAGGGCGTCAGCCGCCGCAGGTGGTGCGTCACCACGAC
GCGCGACGAGCGCGCACGCCCTGCGCCCGACCTGGTCGACCGCAACTTCGCGGCCCCTGGACCCGACCACCTCTGGGTGGCTGACATCACGTACATTCCG
ACGCACGCCGGTTTCCTCTACCTCGCCGTCGTGCTTGACGCATGGAGCCGTAAAGTCGTCGGTTGGGCCATGGCTGGCCACCTCAAGACGGAGCTCGTCG
TGCAGGCACTCGACATGGCTGTGGGGCAGCGTCAGCCCCAAAGCGTCGTGCACCACAGCGACCAAGGGACGCAGTACACCTCGATTGGCTTCGGGCTTCG
GTGCAAGGAAGCCGGCGTGCGCCCATCCATGGGCAGCGTCGGCGACGCGTACGACAACGCGATGTGTGAAAGCTTCTTCGCCACGCTCGAGTGCGAACTG
CTCGCACGCCGCCGCTTCACGTCTCACGCCGAGGCGCGGATGGCCGTCTTCCGCTTCATCGAGGGCTGGTACAATCCGCTCCGGCGCCACTCCGCGCTCG
ACTACGAGTCACCCGCTGGTTACGAGAAGAAGCACCGCGCTGCCGCTTGAGGGCGAAAGCGCGGAACGCTCTGCGGAACCGGAGCAACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 55 | 405 | + | No |
Description : First part of the transposase
ORF sequence :
MPKSRPPYPPEFRERVVELLKAGRSGKSLAREFGCTEQSIRNWKKQLEGSGRSAPSSNGESRPAPRASSAASDERAELERLRREVRVLREERDILKKAAA
WFAQESVATPKRRTDS
WFAQESVATPKRRTDS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 333 | 1250 | + | No |
Description : Second part of the transposase
ORF sequence :
HPKKSCGLVRAGIRGDAQAAYRLVKANQAVHSITTLCRVLEVSESGYHAWRTRLPSKRAVADAGLSKRIRAIHAMSDGTYGVPRIHAELAAEGVHIGRKR
VARLMRAAHLEGVSRRRWCVTTTRDERARPAPDLVDRNFAAPGPDHLWVADITYIPTHAGFLYLAVVLDAWSRKVVGWAMAGHLKTELVVQALDMAVGQR
QPQSVVHHSDQGTQYTSIGFGLRCKEAGVRPSMGSVGDAYDNAMCESFFATLECELLARRRFTSHAEARMAVFRFIEGWYNPLRRHSALDYESPAGYEKK
HRAAA
VARLMRAAHLEGVSRRRWCVTTTRDERARPAPDLVDRNFAAPGPDHLWVADITYIPTHAGFLYLAVVLDAWSRKVVGWAMAGHLKTELVVQALDMAVGQR
QPQSVVHHSDQGTQYTSIGFGLRCKEAGVRPSMGSVGDAYDNAMCESFFATLECELLARRRFTSHAEARMAVFRFIEGWYNPLRRHSALDYESPAGYEKK
HRAAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1196 bp | 398 aa | 55 | 1250 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKSRPPYPPEFRERVVELLKAGRSGKSLAREFGCTEQSIRNWKKQLEGSGRSAPSSNGESRPAPRASSAASDERAELERLRREVRVLREERDILKKSCG
LVRAGIRGDAQAAYRLVKANQAVHSITTLCRVLEVSESGYHAWRTRLPSKRAVADAGLSKRIRAIHAMSDGTYGVPRIHAELAAEGVHIGRKRVARLMRA
AHLEGVSRRRWCVTTTRDERARPAPDLVDRNFAAPGPDHLWVADITYIPTHAGFLYLAVVLDAWSRKVVGWAMAGHLKTELVVQALDMAVGQRQPQSVVH
HSDQGTQYTSIGFGLRCKEAGVRPSMGSVGDAYDNAMCESFFATLECELLARRRFTSHAEARMAVFRFIEGWYNPLRRHSALDYESPAGYEKKHRAAA
LVRAGIRGDAQAAYRLVKANQAVHSITTLCRVLEVSESGYHAWRTRLPSKRAVADAGLSKRIRAIHAMSDGTYGVPRIHAELAAEGVHIGRKRVARLMRA
AHLEGVSRRRWCVTTTRDERARPAPDLVDRNFAAPGPDHLWVADITYIPTHAGFLYLAVVLDAWSRKVVGWAMAGHLKTELVVQALDMAVGQRQPQSVVH
HSDQGTQYTSIGFGLRCKEAGVRPSMGSVGDAYDNAMCESFFATLECELLARRRFTSHAEARMAVFRFIEGWYNPLRRHSALDYESPAGYEKKHRAAA
Blast result :
Comments
ISMxa1 is 56 % (ORFA) aa similar to ISBph1 and 60% (ORFB) aa similar to ISCausp1 The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Nierman,W.C.(2005) Direct submission GenBank.