ISPosp5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000316 | ND | Polaromonas sp. | Polaromonas sp. JS666 |
DNA section
IS Length : 1262 bp
Ends
IR Length : 30/38
IRL : TGGTGGTCTAATTTAAACAGACACAACGATAGGTTTTTTCAAACCTAAAT
IRR : TGGTGGTCAAGTTATTTCGGACACATCGATAGGTTGTTGAGTTGCCGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCTCTACTG | CACTACAAAC | 0 |
DNA sequence
TGGTGGTCTAATTTAAACAGACACAACGATAGGTTTTTTCAAACCTAAATGGCGCAAGACGTGAAAAATCAGAAATCCCGAAGAACCTTTGATGCGAGCT
TCAAGCTCCAGGTGGTTCAGATGATCAAAGCTCAAGGACTGAGCATCGGCCAGGTCTGCAAGGACATGAATCTGGGGGAAAGCGCCGTCAGACGCTGGCT
CACTCAGTTTGAAGCCGAGCAGTCGGGCCAGAGTGGCATTGGCAAACCCATCACGGCAGAGCAACAGCGCATTCGTCAGTTGGAGTGCGAGAACAGGCAA
CTGCGAGGCGATGTCGAAATCTTAAAAAAAGCGTCGGCCTTCTTTGCCCGCGAACTCAAATGAGCTACCAGCTCGTTGAGGACTTGCAAAAGAAGGCTAC
CCCCCAAGTGCCCGTTAGCCAGACGTGCCGAATCCTCGAAATTAGCCGCTCCGGATACTACGCGGCGCGTAAGCGCAGCCAGCAGGCACCCGTGGTGTGC
GCAGCCAGCGTTCATTTGCAAGCCGCTTTCGCGGCCAGTGGCCGCGCTTACGGTAGCCGCAGATTGCGTGCTGCGCTGCACGCACGAGGCGTGACCGTGG
GACGTCACCGGGTACGTAGCCTCATGCGTGCCCATGGGCTGCGCTCGGTCTGGAGGCGCAAGTTTGTGCACACCACGAACAGCAAGCACGGTCTGGCCGT
CTCGCCGAACGTGCTGGACCGGCAGTTCGAGCAAACGGCACCTGATCGGGCTTGGGTTTGCGACATTACCTATATTCGCACGCGTAGTGGCTGGCTGTAT
CTGGCTGCAGTGCTGGACTTGCATTCGCGCAGGATCGTGGGCTGGGCTACTGCTGGAGATATGCAGGCTACGCTGGTGACGACGGCCTTGCAGATAGCCA
TTGCGCAGCGCAATCCCAGTCCTGGGCTAATCGTGCACTCAGACAGGGGCACGCAGTACGCGAGTAGCGCGCATCAGGCGCTGCTGAAAAAGCATGGGCT
GGTAGGTAGCATGAGCCGAAAAGGCAACTGCTGGGATAACGCTGTGATGGAGCGTTTCTTCCTGAATTTGAAAATGGAACGGGTCTGGCAAAAAGACTAC
GCAAATCACAGTGAGGCCACAAACGACGTTGCTGACTACATCGTCGGCTTCTACAACTGTGAACGACTGCATTCAAAACTGGGAAATTTGTCACCCATTG
CCTTCGAGCAAAAATCGGCAACTCAACAACCTATCGATGTGTCCGAAATAACTTGACCACCA
TCAAGCTCCAGGTGGTTCAGATGATCAAAGCTCAAGGACTGAGCATCGGCCAGGTCTGCAAGGACATGAATCTGGGGGAAAGCGCCGTCAGACGCTGGCT
CACTCAGTTTGAAGCCGAGCAGTCGGGCCAGAGTGGCATTGGCAAACCCATCACGGCAGAGCAACAGCGCATTCGTCAGTTGGAGTGCGAGAACAGGCAA
CTGCGAGGCGATGTCGAAATCTTAAAAAAAGCGTCGGCCTTCTTTGCCCGCGAACTCAAATGAGCTACCAGCTCGTTGAGGACTTGCAAAAGAAGGCTAC
CCCCCAAGTGCCCGTTAGCCAGACGTGCCGAATCCTCGAAATTAGCCGCTCCGGATACTACGCGGCGCGTAAGCGCAGCCAGCAGGCACCCGTGGTGTGC
GCAGCCAGCGTTCATTTGCAAGCCGCTTTCGCGGCCAGTGGCCGCGCTTACGGTAGCCGCAGATTGCGTGCTGCGCTGCACGCACGAGGCGTGACCGTGG
GACGTCACCGGGTACGTAGCCTCATGCGTGCCCATGGGCTGCGCTCGGTCTGGAGGCGCAAGTTTGTGCACACCACGAACAGCAAGCACGGTCTGGCCGT
CTCGCCGAACGTGCTGGACCGGCAGTTCGAGCAAACGGCACCTGATCGGGCTTGGGTTTGCGACATTACCTATATTCGCACGCGTAGTGGCTGGCTGTAT
CTGGCTGCAGTGCTGGACTTGCATTCGCGCAGGATCGTGGGCTGGGCTACTGCTGGAGATATGCAGGCTACGCTGGTGACGACGGCCTTGCAGATAGCCA
TTGCGCAGCGCAATCCCAGTCCTGGGCTAATCGTGCACTCAGACAGGGGCACGCAGTACGCGAGTAGCGCGCATCAGGCGCTGCTGAAAAAGCATGGGCT
GGTAGGTAGCATGAGCCGAAAAGGCAACTGCTGGGATAACGCTGTGATGGAGCGTTTCTTCCTGAATTTGAAAATGGAACGGGTCTGGCAAAAAGACTAC
GCAAATCACAGTGAGGCCACAAACGACGTTGCTGACTACATCGTCGGCTTCTACAACTGTGAACGACTGCATTCAAAACTGGGAAATTTGTCACCCATTG
CCTTCGAGCAAAAATCGGCAACTCAACAACCTATCGATGTGTCCGAAATAACTTGACCACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 243 bp | 80 aa | 121 | 363 | + | No |
Description : First part of the transposase
ORF sequence :
MIKAQGLSIGQVCKDMNLGESAVRRWLTQFEAEQSGQSGIGKPITAEQQRIRQLECENRQLRGDVEILKKASAFFARELK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1044 bp | 347 aa | 213 | 1256 | + | No |
Description : Second part of the transposase
ORF sequence :
SRAVGPEWHWQTHHGRATAHSSVGVREQATARRCRNLKKSVGLLCPRTQMSYQLVEDLQKKATPQVPVSQTCRILEISRSGYYAARKRSQQAPVVCAASV
HLQAAFAASGRAYGSRRLRAALHARGVTVGRHRVRSLMRAHGLRSVWRRKFVHTTNSKHGLAVSPNVLDRQFEQTAPDRAWVCDITYIRTRSGWLYLAAV
LDLHSRRIVGWATAGDMQATLVTTALQIAIAQRNPSPGLIVHSDRGTQYASSAHQALLKKHGLVGSMSRKGNCWDNAVMERFFLNLKMERVWQKDYANHS
EATNDVADYIVGFYNCERLHSKLGNLSPIAFEQKSATQQPIDVSEIT
HLQAAFAASGRAYGSRRLRAALHARGVTVGRHRVRSLMRAHGLRSVWRRKFVHTTNSKHGLAVSPNVLDRQFEQTAPDRAWVCDITYIRTRSGWLYLAAV
LDLHSRRIVGWATAGDMQATLVTTALQIAIAQRNPSPGLIVHSDRGTQYASSAHQALLKKHGLVGSMSRKGNCWDNAVMERFFLNLKMERVWQKDYANHS
EATNDVADYIVGFYNCERLHSKLGNLSPIAFEQKSATQQPIDVSEIT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1136 bp | 378 aa | 121 | 1256 | + | Yes |
Chemistry : DDE
ORF sequence :
MIKAQGLSIGQVCKDMNLGESAVRRWLTQFEAEQSGQSGIGKPITAEQQRIRQLECENRQLRGDVEILKKSVGLLCPRTQMSYQLVEDLQKKATPQVPVS
QTCRILEISRSGYYAARKRSQQAPVVCAASVHLQAAFAASGRAYGSRRLRAALHARGVTVGRHRVRSLMRAHGLRSVWRRKFVHTTNSKHGLAVSPNVLD
RQFEQTAPDRAWVCDITYIRTRSGWLYLAAVLDLHSRRIVGWATAGDMQATLVTTALQIAIAQRNPSPGLIVHSDRGTQYASSAHQALLKKHGLVGSMSR
KGNCWDNAVMERFFLNLKMERVWQKDYANHSEATNDVADYIVGFYNCERLHSKLGNLSPIAFEQKSATQQPIDVSEIT
QTCRILEISRSGYYAARKRSQQAPVVCAASVHLQAAFAASGRAYGSRRLRAALHARGVTVGRHRVRSLMRAHGLRSVWRRKFVHTTNSKHGLAVSPNVLD
RQFEQTAPDRAWVCDITYIRTRSGWLYLAAVLDLHSRRIVGWATAGDMQATLVTTALQIAIAQRNPSPGLIVHSDRGTQYASSAHQALLKKHGLVGSMSR
KGNCWDNAVMERFFLNLKMERVWQKDYANHSEATNDVADYIVGFYNCERLHSKLGNLSPIAFEQKSATQQPIDVSEIT
Blast result :
Comments
ISPosp5 is 67 % (ORFA) aa similar to ISBps1 and 59% (ORFB) aa similar to ISApr1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Anderson,I. and Richardson,P.(2006) Direct submission GenBank.