ISNieu2

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008344 ND Nitrosomonas eutropha
Nitrosomonas eutropha C91
DNA section
IS Length : 1265 bp

Ends


IR Length : 29/36

IRL : TGACATGGTTTAATAAACCGGACAGTCCAGTTAAGAGAAAATAACCTTAA
IRR : TGACCTGGTCAAGTAAAACCGGACACTCTAGTTAAGCAACATTTTTCACT

Insertion site


Left flankDirect repeatRight flankDR Length
TGAACTGCTGCCGTGG GTGCGGC3

DNA sequence

TGACATGGTTTAATAAACCGGACAGTCCAGTTAAGAGAAAATAACCTTAACTGGAGGAAGCAATGGAAACGAGAAAGCAGTACACAAAAGAGTTCAAACT
AGATGCCATCAGTCTGGTATTGGATCAGGACCACACACCATCGGAAGTATCCCGAAGCCTGGGAATCAATGCCAATATGCTGAGGAGATGGATCAGAGAA
TATCAGGCAGATAATACGGGTCAGGCATTCCGAGGCAATGGGAAGCTAACACCGGAGCAGGAGGAAATTAGAAAGCTCAAGATCCAGATCAAGCGGCTGG
AAATGGAGAAGCGCATCTTAAAGGAAGCGACGGTCTTTTTCGCGAAAGAAACGAAGTGAAATATTCGTTTATCACCCAAAGGAAAAAGACCTATCCGGTC
GGCTTGATGTGCCGACTCATGGGTGTAAGCCGCAGTGCCTACTATGATTACGTTCGTTGTGCTAACAATTGTTCTACCGACCCGTGCCATATAGAAATAC
TTGGAGCAGTACGGGAGATAGCAAAATCCAGTCATCATAGCTATGGCAGTCGCAGAATCGGAAAAGCGCTGAATGCACGGGGTTATCCGGTTGGTCGCAG
GAAGGCTCGAAGCCTGATGCAAGAAGCAGATGTGCAAGTGAGGCACCGGAAAAAGTACAAGGTCACAACTAACAGTAATCACAAACAACCGATATTTGAG
AATAGGCTGAATCGGCAATTTGATGTTGCAGCCCCCAATCAAATCTACGTTGGTGACATTACCTGCATTTGGACCCGGGAAGGCTGGTTGTATCTGGCGG
TGGTCATTGATTTGTTTTCCAGGAAGGTGGTGGGTTGGAGCATGAGTTCAAGAATGAAAGCAAAGCTGGTTTGTGATGCATTGAGAATGGCTGCCTGGCA
GCGTCAACCACAACCTGGATTGATTGTACATTCAGATCGTGGATCGCAATATGCCAGCAAGCAGTACCGCCAACTTTTAAAAATACACGGCTTTATCGGC
AGTATGAGTCGTAGGGGAAATTGTTGGGATAATTCAGTGGCTGAAAGCTTTTTCGGTAGCCTTAAACGGGAGCAAATCCACTGGCAACATTATCCAACCC
GCCATAAAGCACAACAAGATGTACTGCAGTATATTTCCATGTTCTATAACAGCCAGCGACTGCATTCATACCCGGGTTACAAAAACCCGAATCAATATGA
GATGGAAATGGAAATAGTGAAAAATGTTGCTTAACTAGAGTGTCCGGTTTTACTTGACCAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
297 bp98 aa63359+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

METRKQYTKEFKLDAISLVLDQDHTPSEVSRSLGINANMLRRWIREYQADNTGQAFRGNGKLTPEQEEIRKLKIQIKRLEMEKRILKEATVFFAKETK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
963 bp320 aa2721234+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KAQDPDQAAGNGEAHLKGSDGLFRERNEVKYSFITQRKKTYPVGLMCRLMGVSRSAYYDYVRCANNCSTDPCHIEILGAVREIAKSSHHSYGSRRIGKAL
NARGYPVGRRKARSLMQEADVQVRHRKKYKVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLV
CDALRMAAWQRQPQPGLIVHSDRGSQYASKQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSY
PGYKNPNQYEMEMEIVKNVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1172 bp391 aa631234+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

METRKQYTKEFKLDAISLVLDQDHTPSEVSRSLGINANMLRRWIREYQADNTGQAFRGNGKLTPEQEEIRKLKIQIKRLEMEKRILKKGSDGLFRERNEV
KYSFITQRKKTYPVGLMCRLMGVSRSAYYDYVRCANNCSTDPCHIEILGAVREIAKSSHHSYGSRRIGKALNARGYPVGRRKARSLMQEADVQVRHRKKY
KVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLVCDALRMAAWQRQPQPGLIVHSDRGSQYAS
KQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSYPGYKNPNQYEMEMEIVKNVA

 

Blast result :
Comments
ISNieu2 is 85% (ORFA) and 81% (ORFB) aa similar to ISApr1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Arp,D., Klotz,M., Norton,J., Stein,L., Sayavedra-Soto,L., Hommes,N. and Richardson,P (2006) Direct submission GenBank.