ISNieu2
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_008344 | ND | Nitrosomonas eutropha | Nitrosomonas eutropha C91 |
DNA section
IS Length : 1265 bp
Ends
IR Length : 29/36
IRL : TGACATGGTTTAATAAACCGGACAGTCCAGTTAAGAGAAAATAACCTTAA
IRR : TGACCTGGTCAAGTAAAACCGGACACTCTAGTTAAGCAACATTTTTCACT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TGAACTGCTG | CCG | TGG GTGCGGC | 3 |
DNA sequence
TGACATGGTTTAATAAACCGGACAGTCCAGTTAAGAGAAAATAACCTTAACTGGAGGAAGCAATGGAAACGAGAAAGCAGTACACAAAAGAGTTCAAACT
AGATGCCATCAGTCTGGTATTGGATCAGGACCACACACCATCGGAAGTATCCCGAAGCCTGGGAATCAATGCCAATATGCTGAGGAGATGGATCAGAGAA
TATCAGGCAGATAATACGGGTCAGGCATTCCGAGGCAATGGGAAGCTAACACCGGAGCAGGAGGAAATTAGAAAGCTCAAGATCCAGATCAAGCGGCTGG
AAATGGAGAAGCGCATCTTAAAGGAAGCGACGGTCTTTTTCGCGAAAGAAACGAAGTGAAATATTCGTTTATCACCCAAAGGAAAAAGACCTATCCGGTC
GGCTTGATGTGCCGACTCATGGGTGTAAGCCGCAGTGCCTACTATGATTACGTTCGTTGTGCTAACAATTGTTCTACCGACCCGTGCCATATAGAAATAC
TTGGAGCAGTACGGGAGATAGCAAAATCCAGTCATCATAGCTATGGCAGTCGCAGAATCGGAAAAGCGCTGAATGCACGGGGTTATCCGGTTGGTCGCAG
GAAGGCTCGAAGCCTGATGCAAGAAGCAGATGTGCAAGTGAGGCACCGGAAAAAGTACAAGGTCACAACTAACAGTAATCACAAACAACCGATATTTGAG
AATAGGCTGAATCGGCAATTTGATGTTGCAGCCCCCAATCAAATCTACGTTGGTGACATTACCTGCATTTGGACCCGGGAAGGCTGGTTGTATCTGGCGG
TGGTCATTGATTTGTTTTCCAGGAAGGTGGTGGGTTGGAGCATGAGTTCAAGAATGAAAGCAAAGCTGGTTTGTGATGCATTGAGAATGGCTGCCTGGCA
GCGTCAACCACAACCTGGATTGATTGTACATTCAGATCGTGGATCGCAATATGCCAGCAAGCAGTACCGCCAACTTTTAAAAATACACGGCTTTATCGGC
AGTATGAGTCGTAGGGGAAATTGTTGGGATAATTCAGTGGCTGAAAGCTTTTTCGGTAGCCTTAAACGGGAGCAAATCCACTGGCAACATTATCCAACCC
GCCATAAAGCACAACAAGATGTACTGCAGTATATTTCCATGTTCTATAACAGCCAGCGACTGCATTCATACCCGGGTTACAAAAACCCGAATCAATATGA
GATGGAAATGGAAATAGTGAAAAATGTTGCTTAACTAGAGTGTCCGGTTTTACTTGACCAGGTCA
AGATGCCATCAGTCTGGTATTGGATCAGGACCACACACCATCGGAAGTATCCCGAAGCCTGGGAATCAATGCCAATATGCTGAGGAGATGGATCAGAGAA
TATCAGGCAGATAATACGGGTCAGGCATTCCGAGGCAATGGGAAGCTAACACCGGAGCAGGAGGAAATTAGAAAGCTCAAGATCCAGATCAAGCGGCTGG
AAATGGAGAAGCGCATCTTAAAGGAAGCGACGGTCTTTTTCGCGAAAGAAACGAAGTGAAATATTCGTTTATCACCCAAAGGAAAAAGACCTATCCGGTC
GGCTTGATGTGCCGACTCATGGGTGTAAGCCGCAGTGCCTACTATGATTACGTTCGTTGTGCTAACAATTGTTCTACCGACCCGTGCCATATAGAAATAC
TTGGAGCAGTACGGGAGATAGCAAAATCCAGTCATCATAGCTATGGCAGTCGCAGAATCGGAAAAGCGCTGAATGCACGGGGTTATCCGGTTGGTCGCAG
GAAGGCTCGAAGCCTGATGCAAGAAGCAGATGTGCAAGTGAGGCACCGGAAAAAGTACAAGGTCACAACTAACAGTAATCACAAACAACCGATATTTGAG
AATAGGCTGAATCGGCAATTTGATGTTGCAGCCCCCAATCAAATCTACGTTGGTGACATTACCTGCATTTGGACCCGGGAAGGCTGGTTGTATCTGGCGG
TGGTCATTGATTTGTTTTCCAGGAAGGTGGTGGGTTGGAGCATGAGTTCAAGAATGAAAGCAAAGCTGGTTTGTGATGCATTGAGAATGGCTGCCTGGCA
GCGTCAACCACAACCTGGATTGATTGTACATTCAGATCGTGGATCGCAATATGCCAGCAAGCAGTACCGCCAACTTTTAAAAATACACGGCTTTATCGGC
AGTATGAGTCGTAGGGGAAATTGTTGGGATAATTCAGTGGCTGAAAGCTTTTTCGGTAGCCTTAAACGGGAGCAAATCCACTGGCAACATTATCCAACCC
GCCATAAAGCACAACAAGATGTACTGCAGTATATTTCCATGTTCTATAACAGCCAGCGACTGCATTCATACCCGGGTTACAAAAACCCGAATCAATATGA
GATGGAAATGGAAATAGTGAAAAATGTTGCTTAACTAGAGTGTCCGGTTTTACTTGACCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
297 bp | 98 aa | 63 | 359 | + | No |
Description : First part of the transposase
ORF sequence :
METRKQYTKEFKLDAISLVLDQDHTPSEVSRSLGINANMLRRWIREYQADNTGQAFRGNGKLTPEQEEIRKLKIQIKRLEMEKRILKEATVFFAKETK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
963 bp | 320 aa | 272 | 1234 | + | No |
Description : Second part of the transposase
ORF sequence :
KAQDPDQAAGNGEAHLKGSDGLFRERNEVKYSFITQRKKTYPVGLMCRLMGVSRSAYYDYVRCANNCSTDPCHIEILGAVREIAKSSHHSYGSRRIGKAL
NARGYPVGRRKARSLMQEADVQVRHRKKYKVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLV
CDALRMAAWQRQPQPGLIVHSDRGSQYASKQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSY
PGYKNPNQYEMEMEIVKNVA
NARGYPVGRRKARSLMQEADVQVRHRKKYKVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLV
CDALRMAAWQRQPQPGLIVHSDRGSQYASKQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSY
PGYKNPNQYEMEMEIVKNVA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1172 bp | 391 aa | 63 | 1234 | + | Yes |
Chemistry : DDE
ORF sequence :
METRKQYTKEFKLDAISLVLDQDHTPSEVSRSLGINANMLRRWIREYQADNTGQAFRGNGKLTPEQEEIRKLKIQIKRLEMEKRILKKGSDGLFRERNEV
KYSFITQRKKTYPVGLMCRLMGVSRSAYYDYVRCANNCSTDPCHIEILGAVREIAKSSHHSYGSRRIGKALNARGYPVGRRKARSLMQEADVQVRHRKKY
KVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLVCDALRMAAWQRQPQPGLIVHSDRGSQYAS
KQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSYPGYKNPNQYEMEMEIVKNVA
KYSFITQRKKTYPVGLMCRLMGVSRSAYYDYVRCANNCSTDPCHIEILGAVREIAKSSHHSYGSRRIGKALNARGYPVGRRKARSLMQEADVQVRHRKKY
KVTTNSNHKQPIFENRLNRQFDVAAPNQIYVGDITCIWTREGWLYLAVVIDLFSRKVVGWSMSSRMKAKLVCDALRMAAWQRQPQPGLIVHSDRGSQYAS
KQYRQLLKIHGFIGSMSRRGNCWDNSVAESFFGSLKREQIHWQHYPTRHKAQQDVLQYISMFYNSQRLHSYPGYKNPNQYEMEMEIVKNVA
Blast result :
Comments
ISNieu2 is 85% (ORFA) and 81% (ORFB) aa similar to ISApr1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Arp,D., Klotz,M., Norton,J., Stein,L., Sayavedra-Soto,L., Hommes,N. and Richardson,P (2006) Direct submission GenBank.