ISRhosp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008268 | ND | Rhodococcus sp. | Rhodococcus sp. RHA1 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 24/27
IRL : TGAGGCTGCCCCCTTGGAGTGGACACCCCTGACAATGGTTCTAGGAGAGC
IRR : TGAGGTTGCCCCTTGAGAGTGGACACCGGATTTCATGCGGCAACTGACAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCGAGGCT | AAAT | GTCGGAGGTC | 4 |
DNA sequence
TGAGGCTGCCCCCTTGGAGTGGACACCCCTGACAATGGTTCTAGGAGAGCCAGGAGGGATGTCGAGGTGGGACGCCAGTCTCTGTACACGGAGGAATTCC
GGAAAGACGCGGTCGCGTTGTACCGCGCGGCCGACGGTAAACGCACGTACGCGGATGTGGCCGCGGATGTGGGCGTCAGTGGCGAGACGCTACGGACGTG
GGTCCGCAAGGACACCGCCGCCCGTAACGCGCGCTCGCAGGACCGCAGTACCGGCGCCGACGGGACCGAGGCGGACGAGCTCGCGCGGCTGCGCGCGGAG
AACGCGCGGCTGCGCGGTGCGGAAAAGGAATGGCAGCTCGAACGCGAGATTCTGCGCCGGGCAGCCGCGTATTTCGCAAAGGAGGTGAAGTGAAGACCCG
CCGCTGGGACTTCATCTCCGACCATCGCGCCGAGTTCGGCGTGCAGCGGCTGTGCCGGGCGCTCGGGACCTCCCGTTCCGCCTACTACAAGCACCTGATC
ACGGAGCCGGCCCGAATCGAGCGGCAGGCCGAGGAAGCCGCGACAGTCGCCGAGATCCGCGCAATCCATACCGAGCACCGGAGCGCCTACGGCGCCCCGC
GGGTTCACGCCGAACTCCGCTCCCGCGGACGCAAGATCAACCGCAAACGGGTGACCCGGCTGATGCGAATCCACCACGTGGTGGGCAGGCACCTGCGCCG
CAGCAAGCGCACGACCATCGCGGACAAGTCGGCTCCGTCGGTGCCGGATCTGGTGATGCGAGACTTCACCGCTACGGCTGTCGATACCAAGTGGTGTGGG
GACATCACCTACATCCCGGTGGGATCCTCGTGGTTGTTCTTGGCCACGGTGATCGACATCTGCTCACGCCGGGTGGTGGGCTGGTCGATCGCTGACCACA
TGCGCACCGAGCTCGTCACGGACGCGATCGAGATGGCGGTGCGCACCCGCGGTGGTGACGTCGGTGGCGTTATCTTCCACAGCGATCGCGGATCACAGTA
CACCGCAGCATCTTTCGTCGATGTCTGCCGCCGTCATGGGATTCAGCAAAGTCGAGGACGGGTCGGGTCAAGTTACGATAATGCTCTCGCCGAGTCGTTC
TTCCAAGGACTCAAGAGGGAGTGGCTGCACGGACGGAGTTGGACGTCGAAGTCGCAGGCACGGCTGGAGCTGTTCGAGTGGCTCTCGTACTTCAATCGAC
GTCGCCGTCACTCCGCCCTCGGATACCTCACCCCAGTGGAGTTCGAACAACGACTCATCGCGTCGTCTACGCTGTCAGTTGCCGCATGAAATCCGGTGTC
CACTCTCAAGGGGCAACCTCA
GGAAAGACGCGGTCGCGTTGTACCGCGCGGCCGACGGTAAACGCACGTACGCGGATGTGGCCGCGGATGTGGGCGTCAGTGGCGAGACGCTACGGACGTG
GGTCCGCAAGGACACCGCCGCCCGTAACGCGCGCTCGCAGGACCGCAGTACCGGCGCCGACGGGACCGAGGCGGACGAGCTCGCGCGGCTGCGCGCGGAG
AACGCGCGGCTGCGCGGTGCGGAAAAGGAATGGCAGCTCGAACGCGAGATTCTGCGCCGGGCAGCCGCGTATTTCGCAAAGGAGGTGAAGTGAAGACCCG
CCGCTGGGACTTCATCTCCGACCATCGCGCCGAGTTCGGCGTGCAGCGGCTGTGCCGGGCGCTCGGGACCTCCCGTTCCGCCTACTACAAGCACCTGATC
ACGGAGCCGGCCCGAATCGAGCGGCAGGCCGAGGAAGCCGCGACAGTCGCCGAGATCCGCGCAATCCATACCGAGCACCGGAGCGCCTACGGCGCCCCGC
GGGTTCACGCCGAACTCCGCTCCCGCGGACGCAAGATCAACCGCAAACGGGTGACCCGGCTGATGCGAATCCACCACGTGGTGGGCAGGCACCTGCGCCG
CAGCAAGCGCACGACCATCGCGGACAAGTCGGCTCCGTCGGTGCCGGATCTGGTGATGCGAGACTTCACCGCTACGGCTGTCGATACCAAGTGGTGTGGG
GACATCACCTACATCCCGGTGGGATCCTCGTGGTTGTTCTTGGCCACGGTGATCGACATCTGCTCACGCCGGGTGGTGGGCTGGTCGATCGCTGACCACA
TGCGCACCGAGCTCGTCACGGACGCGATCGAGATGGCGGTGCGCACCCGCGGTGGTGACGTCGGTGGCGTTATCTTCCACAGCGATCGCGGATCACAGTA
CACCGCAGCATCTTTCGTCGATGTCTGCCGCCGTCATGGGATTCAGCAAAGTCGAGGACGGGTCGGGTCAAGTTACGATAATGCTCTCGCCGAGTCGTTC
TTCCAAGGACTCAAGAGGGAGTGGCTGCACGGACGGAGTTGGACGTCGAAGTCGCAGGCACGGCTGGAGCTGTTCGAGTGGCTCTCGTACTTCAATCGAC
GTCGCCGTCACTCCGCCCTCGGATACCTCACCCCAGTGGAGTTCGAACAACGACTCATCGCGTCGTCTACGCTGTCAGTTGCCGCATGAAATCCGGTGTC
CACTCTCAAGGGGCAACCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 67 | 393 | + | No |
Description : First part of the transposase
ORF sequence :
MGRQSLYTEEFRKDAVALYRAADGKRTYADVAADVGVSGETLRTWVRKDTAARNARSQDRSTGADGTEADELARLRAENARLRGAEKEWQLEREILRRAA
AYFAKEVK
AYFAKEVK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1062 bp | 353 aa | 228 | 1289 | + | No |
Description : Second part of the transposase
ORF sequence :
RALAGPQYRRRRDRGGRARAAARGERAAARCGKGMAARTRDSAPGSRVFRKGGEVKTRRWDFISDHRAEFGVQRLCRALGTSRSAYYKHLITEPARIERQ
AEEAATVAEIRAIHTEHRSAYGAPRVHAELRSRGRKINRKRVTRLMRIHHVVGRHLRRSKRTTIADKSAPSVPDLVMRDFTATAVDTKWCGDITYIPVGS
SWLFLATVIDICSRRVVGWSIADHMRTELVTDAIEMAVRTRGGDVGGVIFHSDRGSQYTAASFVDVCRRHGIQQSRGRVGSSYDNALAESFFQGLKREWL
HGRSWTSKSQARLELFEWLSYFNRRRRHSALGYLTPVEFEQRLIASSTLSVAA
AEEAATVAEIRAIHTEHRSAYGAPRVHAELRSRGRKINRKRVTRLMRIHHVVGRHLRRSKRTTIADKSAPSVPDLVMRDFTATAVDTKWCGDITYIPVGS
SWLFLATVIDICSRRVVGWSIADHMRTELVTDAIEMAVRTRGGDVGGVIFHSDRGSQYTAASFVDVCRRHGIQQSRGRVGSSYDNALAESFFQGLKREWL
HGRSWTSKSQARLELFEWLSYFNRRRRHSALGYLTPVEFEQRLIASSTLSVAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1223 bp | 407 aa | 67 | 1289 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRQSLYTEEFRKDAVALYRAADGKRTYADVAADVGVSGETLRTWVRKDTAARNARSQDRSTGADGTEADELARLRAENARLRGAEKGMAARTRDSAPGS
RVFRKGGEVKTRRWDFISDHRAEFGVQRLCRALGTSRSAYYKHLITEPARIERQAEEAATVAEIRAIHTEHRSAYGAPRVHAELRSRGRKINRKRVTRLM
RIHHVVGRHLRRSKRTTIADKSAPSVPDLVMRDFTATAVDTKWCGDITYIPVGSSWLFLATVIDICSRRVVGWSIADHMRTELVTDAIEMAVRTRGGDVG
GVIFHSDRGSQYTAASFVDVCRRHGIQQSRGRVGSSYDNALAESFFQGLKREWLHGRSWTSKSQARLELFEWLSYFNRRRRHSALGYLTPVEFEQRLIAS
STLSVAA
RVFRKGGEVKTRRWDFISDHRAEFGVQRLCRALGTSRSAYYKHLITEPARIERQAEEAATVAEIRAIHTEHRSAYGAPRVHAELRSRGRKINRKRVTRLM
RIHHVVGRHLRRSKRTTIADKSAPSVPDLVMRDFTATAVDTKWCGDITYIPVGSSWLFLATVIDICSRRVVGWSIADHMRTELVTDAIEMAVRTRGGDVG
GVIFHSDRGSQYTAASFVDVCRRHGIQQSRGRVGSSYDNALAESFFQGLKREWLHGRSWTSKSQARLELFEWLSYFNRRRRHSALGYLTPVEFEQRLIAS
STLSVAA
Blast result :
Comments
ISRhosp1 is 62 % (ORFA) aa similar to IS1206 and 55% (ORFB) aa similar to ISRasp1 .The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] McLeod,M.P. (2006) Proc. Natl. Acad. Sci. U.S.A. In press