ISPca2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007498 | ND | Pelobacter carbinolicus | Pelobacter carbinolicus DSM 2380 |
DNA section
IS Length : 1218 bp
Ends
IR Length : 22/43
IRL : TGAGGTATAAAAAAATCAGACTTCAGGAGGGACCCATGAGCAGCAAACGT
IRR : TGAAGCAAAAAGAAAAGTGAATCGAACCCGGTTTGTTAGACACTCTTGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGTAGACACTCC | GTATATCTGAGGC | 0 |
DNA sequence
TGAGGTATAAAAAAATCAGACTTCAGGAGGGACCCATGAGCAGCAAACGTTACACCGAAGAGTTCAAGATCGAAGCCGTCAAGCAAGTCACGGAATGCGG
CTATTCCGCTTCCGAAGTTGCCCGGCGTTTGGGTGTGAATGCCCACAGCCTTTACGCCTGGATCAAAAAATACAGCCAAGGCCCGACCCATGCGGCTAAG
GTCGATGACCAGCAGGCTGAGATCAACCGCCTTCGCAAGGAGCTCAAGCGCGTCACCGAAGAACGAGACATCCTAAAAAAGGCCACCGCGTACTTCGCCA
AAGAGTCCCAGTGAGGTACGCCTTCATCCGGGCGCATCAAGAACAGTTTCCCTTGCGCACCCTTTGCCGCATGATGAGCGTCCATCGCAGCGGTTACTAT
GCCTGGAGCAAACAGCCCAAGTCCCCTCGGCAAAAGGAGGACAAGCGACTTACGGGACTCATCAAGCAAGCCTGGCTGGAAAGTGGCTGCATCTACGGTT
ATCGCAAAATCCATGACGACCTTCGGGAACTCGGGGAAACCTGTGGCCGGAATCGGGTAGCCCGCATCATGAAGCAGGCCGGCATCAAGGCCCAGGTCGG
ATACCGCAAGCCGCGCCCCCAAAACGGCAAAGTCTCTGTTTTGGCCGACAATCACCTCAACCAGAACTTCGAGGCCGGGAAGCCAAATCAGGTTTGGGTT
ACAGATATCACCTATATCCGCACCTACGAAGGCTGGCTGTTTCTGGCCGTCGTGATCGACCTGTTCAACCGCCAGGTCGTGGGCTGGTCCATGCAGCCAC
AGATGCATGCCGATCTGGCGCTGAAGGCTCTGCTAATGGCTATGTGGCGACGCAAACCGGAGAACGAGGTCCTGATTCACTCGGACCAAGGCACCCAGTA
CACCAGCGGCGACTGGCAGAGTTTTTTGAAAGCACACAGGCTGGTTTGCAGCATGAGCCGGCGCGGCAACTGCTACGACAATGCGGTAGCCGAGAGTTTC
TTCCAGTTACTAAAACGCGAACGGATCAAACGCAGAATCTACAAAACCCGTGAGGACGCCCGCCGGGATGTCTTCGACTACATAGAAATGTTTTACAACC
CGGTCCGCCGCCACGGCAACAACGGCAACCTCTCCCCGGTCGAATTCGAGAGGCAGTATTTTCAGCAGGCCAAGAGTGTCTAACAAACCGGGTTCGATTC
ACTTTTCTTTTTGCTTCA
CTATTCCGCTTCCGAAGTTGCCCGGCGTTTGGGTGTGAATGCCCACAGCCTTTACGCCTGGATCAAAAAATACAGCCAAGGCCCGACCCATGCGGCTAAG
GTCGATGACCAGCAGGCTGAGATCAACCGCCTTCGCAAGGAGCTCAAGCGCGTCACCGAAGAACGAGACATCCTAAAAAAGGCCACCGCGTACTTCGCCA
AAGAGTCCCAGTGAGGTACGCCTTCATCCGGGCGCATCAAGAACAGTTTCCCTTGCGCACCCTTTGCCGCATGATGAGCGTCCATCGCAGCGGTTACTAT
GCCTGGAGCAAACAGCCCAAGTCCCCTCGGCAAAAGGAGGACAAGCGACTTACGGGACTCATCAAGCAAGCCTGGCTGGAAAGTGGCTGCATCTACGGTT
ATCGCAAAATCCATGACGACCTTCGGGAACTCGGGGAAACCTGTGGCCGGAATCGGGTAGCCCGCATCATGAAGCAGGCCGGCATCAAGGCCCAGGTCGG
ATACCGCAAGCCGCGCCCCCAAAACGGCAAAGTCTCTGTTTTGGCCGACAATCACCTCAACCAGAACTTCGAGGCCGGGAAGCCAAATCAGGTTTGGGTT
ACAGATATCACCTATATCCGCACCTACGAAGGCTGGCTGTTTCTGGCCGTCGTGATCGACCTGTTCAACCGCCAGGTCGTGGGCTGGTCCATGCAGCCAC
AGATGCATGCCGATCTGGCGCTGAAGGCTCTGCTAATGGCTATGTGGCGACGCAAACCGGAGAACGAGGTCCTGATTCACTCGGACCAAGGCACCCAGTA
CACCAGCGGCGACTGGCAGAGTTTTTTGAAAGCACACAGGCTGGTTTGCAGCATGAGCCGGCGCGGCAACTGCTACGACAATGCGGTAGCCGAGAGTTTC
TTCCAGTTACTAAAACGCGAACGGATCAAACGCAGAATCTACAAAACCCGTGAGGACGCCCGCCGGGATGTCTTCGACTACATAGAAATGTTTTACAACC
CGGTCCGCCGCCACGGCAACAACGGCAACCTCTCCCCGGTCGAATTCGAGAGGCAGTATTTTCAGCAGGCCAAGAGTGTCTAACAAACCGGGTTCGATTC
ACTTTTCTTTTTGCTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 36 | 314 | + | No |
Description : First part of the transposase
ORF sequence :
MSSKRYTEEFKIEAVKQVTECGYSASEVARRLGVNAHSLYAWIKKYSQGPTHAAKVDDQQAEINRLRKELKRVTEERDILKKATAYFAKESQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 221 | 1183 | + | No |
Description : Second part of the transposase
ORF sequence :
DQPPSQGAQARHRRTRHPKKGHRVLRQRVPVRYAFIRAHQEQFPLRTLCRMMSVHRSGYYAWSKQPKSPRQKEDKRLTGLIKQAWLESGCIYGYRKIHDD
LRELGETCGRNRVARIMKQAGIKAQVGYRKPRPQNGKVSVLADNHLNQNFEAGKPNQVWVTDITYIRTYEGWLFLAVVIDLFNRQVVGWSMQPQMHADLA
LKALLMAMWRRKPENEVLIHSDQGTQYTSGDWQSFLKAHRLVCSMSRRGNCYDNAVAESFFQLLKRERIKRRIYKTREDARRDVFDYIEMFYNPVRRHGN
NGNLSPVEFERQYFQQAKSV
LRELGETCGRNRVARIMKQAGIKAQVGYRKPRPQNGKVSVLADNHLNQNFEAGKPNQVWVTDITYIRTYEGWLFLAVVIDLFNRQVVGWSMQPQMHADLA
LKALLMAMWRRKPENEVLIHSDQGTQYTSGDWQSFLKAHRLVCSMSRRGNCYDNAVAESFFQLLKRERIKRRIYKTREDARRDVFDYIEMFYNPVRRHGN
NGNLSPVEFERQYFQQAKSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 36 | 1183 | + | Yes |
Chemistry : DDE
ORF sequence :
MSSKRYTEEFKIEAVKQVTECGYSASEVARRLGVNAHSLYAWIKKYSQGPTHAAKVDDQQAEINRLRKELKRVTEERDILKKGHRVLRQRVPVRYAFIRA
HQEQFPLRTLCRMMSVHRSGYYAWSKQPKSPRQKEDKRLTGLIKQAWLESGCIYGYRKIHDDLRELGETCGRNRVARIMKQAGIKAQVGYRKPRPQNGKV
SVLADNHLNQNFEAGKPNQVWVTDITYIRTYEGWLFLAVVIDLFNRQVVGWSMQPQMHADLALKALLMAMWRRKPENEVLIHSDQGTQYTSGDWQSFLKA
HRLVCSMSRRGNCYDNAVAESFFQLLKRERIKRRIYKTREDARRDVFDYIEMFYNPVRRHGNNGNLSPVEFERQYFQQAKSV
HQEQFPLRTLCRMMSVHRSGYYAWSKQPKSPRQKEDKRLTGLIKQAWLESGCIYGYRKIHDDLRELGETCGRNRVARIMKQAGIKAQVGYRKPRPQNGKV
SVLADNHLNQNFEAGKPNQVWVTDITYIRTYEGWLFLAVVIDLFNRQVVGWSMQPQMHADLALKALLMAMWRRKPENEVLIHSDQGTQYTSGDWQSFLKA
HRLVCSMSRRGNCYDNAVAESFFQLLKRERIKRRIYKTREDARRDVFDYIEMFYNPVRRHGNNGNLSPVEFERQYFQQAKSV
Blast result :
Comments
ISPca2 is 77 % (ORFA) aa similar to ISXac3 and 79% (ORFB) aa similar to ISAisp2. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Chertkov,O., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Lovley,D.R., Blunt-Harris,E.L., DiDonato,R., Muktak,A., Haveman,S., Butler,J., Chaudhuri,S.K., DiDonato,L.N., Ding,Y.R., Glaven,R., Segura-Gonzalez,D., Izallalen,M., Kaye,J., Kim,B.C., Juarez-Lopez,K., Mester,T., Postier,B., Risso,C., Tran,H., Brown,P., Nicoll,J.S., Huang,K., Keller,K., Alm,E. And Richardson,P. (2006) Direct Submission GenBank.