ISLmo1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003210 | ND | Listeria monocytogenes | Listeria monocytogenes EGD-e |
DNA section
IS Length : 1228 bp
Ends
IR Length : 33/49
IRL : TGAGAATAAGATAAAAACACCTTCGTTGGATTCATGTAAAATGAAACTAA
IRR : TGAGAATGAAATAAAAACCCTTTAAATTAGGCTGCTTGTTTCCTGAAATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACTAAAAAA | TTTTTCCATG | 0 |
DNA sequence
TGAGAATAAGATAAAAACACCTTCGTTGGATTCATGTAAAATGAAACTAACGGAGGTGTTTCTATGAGTACAAGAGTAATGTATCCAGCAGAAATAAAAG
AAAAAGCTATCAAAATGAAATTAGCAGGTAAATCTACCAAAGAAATTATGAGAACATTAAACATCAAAAATCCAACACAAGTGAAAACTTGGTGGCGCTG
GTATCGAAATGAAGAAACCCACAGGTTTCACCAAGGAGTAGGTAAACAATATAAATACCAAAAAGGGGTGCCTGAATTACCAGAAATAGAACAATTAAAA
ATAGCTCTTCGGCAAAAAGAGCTAGAACTGGAAATTTTAAAAAAGTACAAGGCATTGGAAAGGAAGTGAATCCAGCATATTTTGTTTTGTTAGTAGAAGA
GCTAAAAACAAAGTATCTTGTCAAAGATATCTGTCAGATACTACAAGTACCTTTATCTACCTATTATCGCTGGAAAAAGAAAGATTTCTCACAGAGCTCA
TTGGAGAAAAAAATAGGGCAGTTATGTAAAGCATATCAGTTCACTTATGGTTATCGAAAAATCGCCGCATTAATGAAACAAGAAGAACAAGTTGGCATCA
ATAAAGTACAGCGTATTATGCAAAAATATCAGTGGCAATGTCAGGTAAAAGTAAAAAAGAAAAATCGACCAGGCTCCGCCTACTATCAGACAGAAAACCA
CTTAAATCGAAACTTTCAAGCTAGTCAGCCGCTGGAAAAATTAACTACCGATATCACCTACCTTTATTTCGGAGACTGCCGTTTATATTTATCAAGCATT
ATGGATTTGTATAATGGAGAAATCGTTGCCTACTCCATTGGCGAAAAACAAGACACAGAATTAGTGCTAGATACATTAAATCAGTTGTCCCTTCCAGAAG
GAAGTTTACTTCATAGCGATCAAGGTTCGGTGTACACATCTTATGAATATTATCAGTGCTGTAAAAAGAAAAACGTTATCCGCAGTATGTCCCGAAAAGG
GACACCCGCAGACAACGCTCCCATAGAATGTTTCCATTCCTCGCTAAAGTGCGAAACATTCTACCATAAAACAGCGTATAAATACGCTAAATCTATTGTA
ATCCAAATCGTAAAAGATTACATTAAATATTATAACGAAAAACGAATTCAACAAAAATTAGGCTACCAATCTCCTATTAATTTCAGGAAACAAGCAGCCT
AATTTAAAGGGTTTTTATTTCATTCTCA
AAAAAGCTATCAAAATGAAATTAGCAGGTAAATCTACCAAAGAAATTATGAGAACATTAAACATCAAAAATCCAACACAAGTGAAAACTTGGTGGCGCTG
GTATCGAAATGAAGAAACCCACAGGTTTCACCAAGGAGTAGGTAAACAATATAAATACCAAAAAGGGGTGCCTGAATTACCAGAAATAGAACAATTAAAA
ATAGCTCTTCGGCAAAAAGAGCTAGAACTGGAAATTTTAAAAAAGTACAAGGCATTGGAAAGGAAGTGAATCCAGCATATTTTGTTTTGTTAGTAGAAGA
GCTAAAAACAAAGTATCTTGTCAAAGATATCTGTCAGATACTACAAGTACCTTTATCTACCTATTATCGCTGGAAAAAGAAAGATTTCTCACAGAGCTCA
TTGGAGAAAAAAATAGGGCAGTTATGTAAAGCATATCAGTTCACTTATGGTTATCGAAAAATCGCCGCATTAATGAAACAAGAAGAACAAGTTGGCATCA
ATAAAGTACAGCGTATTATGCAAAAATATCAGTGGCAATGTCAGGTAAAAGTAAAAAAGAAAAATCGACCAGGCTCCGCCTACTATCAGACAGAAAACCA
CTTAAATCGAAACTTTCAAGCTAGTCAGCCGCTGGAAAAATTAACTACCGATATCACCTACCTTTATTTCGGAGACTGCCGTTTATATTTATCAAGCATT
ATGGATTTGTATAATGGAGAAATCGTTGCCTACTCCATTGGCGAAAAACAAGACACAGAATTAGTGCTAGATACATTAAATCAGTTGTCCCTTCCAGAAG
GAAGTTTACTTCATAGCGATCAAGGTTCGGTGTACACATCTTATGAATATTATCAGTGCTGTAAAAAGAAAAACGTTATCCGCAGTATGTCCCGAAAAGG
GACACCCGCAGACAACGCTCCCATAGAATGTTTCCATTCCTCGCTAAAGTGCGAAACATTCTACCATAAAACAGCGTATAAATACGCTAAATCTATTGTA
ATCCAAATCGTAAAAGATTACATTAAATATTATAACGAAAAACGAATTCAACAAAAATTAGGCTACCAATCTCCTATTAATTTCAGGAAACAAGCAGCCT
AATTTAAAGGGTTTTTATTTCATTCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 83 | 388 | + | No |
Description : First part of the transposase
ORF sequence :
MSTRVMYPAEIKEKAIKMKLAGKSTKEIMRTLNIKNPTQVKTWWRWYRNEETHRFHQGVGKQYKYQKGVPELPEIEQLKIALRQKELELEILKKYKALER
K
K
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 295 | 1221 | + | No |
Description : Second part of the transposase
ORF sequence :
ITRNRTIKNSSSAKRARTGNFKKVQGIGKEVNPAYFVLLVEELKTKYLVKDICQILQVPLSTYYRWKKKDFSQSSLEKKIGQLCKAYQFTYGYRKIAALM
KQEEQVGINKVQRIMQKYQWQCQVKVKKKNRPGSAYYQTENHLNRNFQASQPLEKLTTDITYLYFGDCRLYLSSIMDLYNGEIVAYSIGEKQDTELVLDT
LNQLSLPEGSLLHSDQGSVYTSYEYYQCCKKKNVIRSMSRKGTPADNAPIECFHSSLKCETFYHKTAYKYAKSIVIQIVKDYIKYYNEKRIQQKLGYQSP
INFRKQAA
KQEEQVGINKVQRIMQKYQWQCQVKVKKKNRPGSAYYQTENHLNRNFQASQPLEKLTTDITYLYFGDCRLYLSSIMDLYNGEIVAYSIGEKQDTELVLDT
LNQLSLPEGSLLHSDQGSVYTSYEYYQCCKKKNVIRSMSRKGTPADNAPIECFHSSLKCETFYHKTAYKYAKSIVIQIVKDYIKYYNEKRIQQKLGYQSP
INFRKQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 83 | 1221 | + | Yes |
Chemistry : DDE
ORF sequence :
MSTRVMYPAEIKEKAIKMKLAGKSTKEIMRTLNIKNPTQVKTWWRWYRNEETHRFHQGVGKQYKYQKGVPELPEIEQLKIALRQKELELEILKKVQGIGK
EVNPAYFVLLVEELKTKYLVKDICQILQVPLSTYYRWKKKDFSQSSLEKKIGQLCKAYQFTYGYRKIAALMKQEEQVGINKVQRIMQKYQWQCQVKVKKK
NRPGSAYYQTENHLNRNFQASQPLEKLTTDITYLYFGDCRLYLSSIMDLYNGEIVAYSIGEKQDTELVLDTLNQLSLPEGSLLHSDQGSVYTSYEYYQCC
KKKNVIRSMSRKGTPADNAPIECFHSSLKCETFYHKTAYKYAKSIVIQIVKDYIKYYNEKRIQQKLGYQSPINFRKQAA
EVNPAYFVLLVEELKTKYLVKDICQILQVPLSTYYRWKKKDFSQSSLEKKIGQLCKAYQFTYGYRKIAALMKQEEQVGINKVQRIMQKYQWQCQVKVKKK
NRPGSAYYQTENHLNRNFQASQPLEKLTTDITYLYFGDCRLYLSSIMDLYNGEIVAYSIGEKQDTELVLDTLNQLSLPEGSLLHSDQGSVYTSYEYYQCC
KKKNVIRSMSRKGTPADNAPIECFHSSLKCETFYHKTAYKYAKSIVIQIVKDYIKYYNEKRIQQKLGYQSPINFRKQAA
Blast result :
Comments
ISLmo1 is 81% (ORFA) and 72% (ORFB) aa similar to ISSau4. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Glaser,P., Frangeul,L., Buchrieser,C., Amend,A., Baquero,F., Berche,P., Bloecker,H., Brandt,P., Chakraborty,T., Charbit,A., Chetouani,F., Couve,E., de Daruvar,A., Dehoux,P., Domann,E., Dominguez-Bernal,G., Duchaud,E., Durand,L., Dussurget,O., Entian,K.-D., Fsihi,H., Garcia-Del Portillo,F., Garrido,P., Gautier,L., Goebel,W., Gomez-Lopez,N., Hain,T., Hauf,J., Jackson,D., Jones,L.-M., Karst,U., Kreft,J., Kuhn,M., Kunst,F., Kurapkat,G., Madueno,E., Maitournam,A., Mata Vicente,J., Ng,E., Nordsiek,G., Novella,S., de Pablos,B., Perez-Diaz,J.-C., Remmel,B., Rose,M., Rusniok,C., Schlueter,T., Simoes,N., Tierrez,A., Vazquez-Boland,J.-A., Voss,H., Wehland,J. and Cossart,P. (2001) Science 294 (5543), 849-852.