ISLpn7
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006369 | ND | Legionella pneumophila | Legionella pneumophila Lens |
DNA section
IS Length : 1269 bp
Ends
IR Length : 28/37
IRL : TGATGTGGTTCAATTGATTTAGACACTCCAGTTAAGAGATAATTTGTTTA
IRR : TGACGTGGTCAAGCAAATTTGTACACCCCAGTTAAGCCACTTTTTTCATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAACGTTCA | ATA | GTTTGCTGGTGT | 3 |
DNA sequence
TGATGTGGTTCAATTGATTTAGACACTCCAGTTAAGAGATAATTTGTTTAATTGGAGGTCACAATGTCTATAAGAAGAAAATATACAAAGGAATTTAAAC
TGGATGCGATTAGTTTAGTAACTGAGCAAGGATACACATGTCCAGAAGCAGCAAGGAGCTTGGGAATTAATTCGAATATTTTGAGTCGCTGGATTCGAGA
GGCAGCGGAGAATAATGAAAATGCTTTTCGTGGAAATGGAAAATTAACGGAAGAGCAACTTGAAATTCGTCAGTTACGAGAAGAAGTCAGAAGGCTGACA
ATGGAGAAAGAAATACTAAAAAAAGCCACGGTCTTCTTTGCGAAAGAAGCGAAATAAAATATTCGTTTATCGCCCAACATAAGAAGACCTGGCCGGTTGG
CATGATGTGCCAACTCATGGGCGTTACTTCTTCCGGGTACTATAGCTATCAAAAACGGAAGAGTACAAAAACGAACAATGAACCAGAACATGAAGAGCTA
TTGGAGTGGGTTAAAAAAATATCGGAATCCAGCAAGTTTTCGTATGGAAATCGCAGGATAAGGAAAGCACTGAATGCTCTTGGTTATCCGGTTGGCCGCA
GAAAAACCCATAGATTAATGCGTGAAGCGGGTATTTTTGTTCGCTATAAAAAGAAATACAAAGTGACAACAAACAGCAATCACAAGCAACCTATTTTTGA
TAATGTTTTAAACAGGAAATTTCAGGTCAATGAACCCAATCGTGCCTATGTGTCCGATATGACCTATATCCCAACCCATGAGGGCTGGTTATACCTGACG
GTGGTAATTGATTTGTTTTCCCGAAAAGTGGTTGGTTGGAACATGAGTTCGAGAATGAAGGCTGATACAGTTTGCGATGCATTAACAATGGCCATTTGGC
AGAGAAAGCCAAAAGCAGGCCTTATTGTGCATTCCGACAGGGGCTCACAATATGCCAGTAAACAATATCGCAGCCTTCTCAATCAACATGGCCTTGTGGG
CAGCATGAGCAAAAAAGGTGACTGCTGGGACAATAGCGTTGCTGAGAGTTTTTTCAGCCACTTGAAGGATGAATTAGTTCATTGGCGTAATTATCAAACC
AGACGGGAGGCAAAACAAGATATTCTAGATTACATGACTATGTTTTATAACAATCAAAGATTGCATTCGTCTTTGGATTATCTAAGTCCGAATCAATTTG
AAAATCGGTACTGGGGGTTAATGAAAAAAGTGGCTTAACTGGGGTGTACAAATTTGCTTGACCACGTCA
TGGATGCGATTAGTTTAGTAACTGAGCAAGGATACACATGTCCAGAAGCAGCAAGGAGCTTGGGAATTAATTCGAATATTTTGAGTCGCTGGATTCGAGA
GGCAGCGGAGAATAATGAAAATGCTTTTCGTGGAAATGGAAAATTAACGGAAGAGCAACTTGAAATTCGTCAGTTACGAGAAGAAGTCAGAAGGCTGACA
ATGGAGAAAGAAATACTAAAAAAAGCCACGGTCTTCTTTGCGAAAGAAGCGAAATAAAATATTCGTTTATCGCCCAACATAAGAAGACCTGGCCGGTTGG
CATGATGTGCCAACTCATGGGCGTTACTTCTTCCGGGTACTATAGCTATCAAAAACGGAAGAGTACAAAAACGAACAATGAACCAGAACATGAAGAGCTA
TTGGAGTGGGTTAAAAAAATATCGGAATCCAGCAAGTTTTCGTATGGAAATCGCAGGATAAGGAAAGCACTGAATGCTCTTGGTTATCCGGTTGGCCGCA
GAAAAACCCATAGATTAATGCGTGAAGCGGGTATTTTTGTTCGCTATAAAAAGAAATACAAAGTGACAACAAACAGCAATCACAAGCAACCTATTTTTGA
TAATGTTTTAAACAGGAAATTTCAGGTCAATGAACCCAATCGTGCCTATGTGTCCGATATGACCTATATCCCAACCCATGAGGGCTGGTTATACCTGACG
GTGGTAATTGATTTGTTTTCCCGAAAAGTGGTTGGTTGGAACATGAGTTCGAGAATGAAGGCTGATACAGTTTGCGATGCATTAACAATGGCCATTTGGC
AGAGAAAGCCAAAAGCAGGCCTTATTGTGCATTCCGACAGGGGCTCACAATATGCCAGTAAACAATATCGCAGCCTTCTCAATCAACATGGCCTTGTGGG
CAGCATGAGCAAAAAAGGTGACTGCTGGGACAATAGCGTTGCTGAGAGTTTTTTCAGCCACTTGAAGGATGAATTAGTTCATTGGCGTAATTATCAAACC
AGACGGGAGGCAAAACAAGATATTCTAGATTACATGACTATGTTTTATAACAATCAAAGATTGCATTCGTCTTTGGATTATCTAAGTCCGAATCAATTTG
AAAATCGGTACTGGGGGTTAATGAAAAAAGTGGCTTAACTGGGGTGTACAAATTTGCTTGACCACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 295 bp | 97 aa | 64 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MSIRRKYTKEFKLDAISLVTEQGYTCPEAARSLGINSNILSRWIREAAENNENAFRGNGKLTEEQLEIRQLREEVRRLTMEKEILKKATVFFAKEAK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 975 bp | 324 aa | 264 | 1238 | + | No |
Description : Second part of the transposase
ORF sequence :
NSSVTRRSQKADNGERNTKKSHGLLCERSEIKYSFIAQHKKTWPVGMMCQLMGVTSSGYYSYQKRKSTKTNNEPEHEELLEWVKKISESSKFSYGNRRIR
KALNALGYPVGRRKTHRLMREAGIFVRYKKKYKVTTNSNHKQPIFDNVLNRKFQVNEPNRAYVSDMTYIPTHEGWLYLTVVIDLFSRKVVGWNMSSRMKA
DTVCDALTMAIWQRKPKAGLIVHSDRGSQYASKQYRSLLNQHGLVGSMSKKGDCWDNSVAESFFSHLKDELVHWRNYQTRREAKQDILDYMTMFYNNQRL
HSSLDYLSPNQFENRYWGLMKKVA
KALNALGYPVGRRKTHRLMREAGIFVRYKKKYKVTTNSNHKQPIFDNVLNRKFQVNEPNRAYVSDMTYIPTHEGWLYLTVVIDLFSRKVVGWNMSSRMKA
DTVCDALTMAIWQRKPKAGLIVHSDRGSQYASKQYRSLLNQHGLVGSMSKKGDCWDNSVAESFFSHLKDELVHWRNYQTRREAKQDILDYMTMFYNNQRL
HSSLDYLSPNQFENRYWGLMKKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 391 aa | 64 | 1235 | + | Yes |
Chemistry : DDE
ORF sequence :
MSIRRKYTKEFKLDAISLVTEQGYTCPEAARSLGINSNILSRWIREAAENNENAFRGNGKLTEEQLEIRQLREEVRRLTMEKEILKKSHGLLCERSEIKY
SFIAQHKKTWPVGMMCQLMGVTSSGYYSYQKRKSTKTNNEPEHEELLEWVKKISESSKFSYGNRRIRKALNALGYPVGRRKTHRLMREAGIFVRYKKKYK
VTTNSNHKQPIFDNVLNRKFQVNEPNRAYVSDMTYIPTHEGWLYLTVVIDLFSRKVVGWNMSSRMKADTVCDALTMAIWQRKPKAGLIVHSDRGSQYASK
QYRSLLNQHGLVGSMSKKGDCWDNSVAESFFSHLKDELVHWRNYQTRREAKQDILDYMTMFYNNQRLHSSLDYLSPNQFENRYWGLMKKVA
SFIAQHKKTWPVGMMCQLMGVTSSGYYSYQKRKSTKTNNEPEHEELLEWVKKISESSKFSYGNRRIRKALNALGYPVGRRKTHRLMREAGIFVRYKKKYK
VTTNSNHKQPIFDNVLNRKFQVNEPNRAYVSDMTYIPTHEGWLYLTVVIDLFSRKVVGWNMSSRMKADTVCDALTMAIWQRKPKAGLIVHSDRGSQYASK
QYRSLLNQHGLVGSMSKKGDCWDNSVAESFFSHLKDELVHWRNYQTRREAKQDILDYMTMFYNNQRLHSSLDYLSPNQFENRYWGLMKKVA
Blast result :
Comments
ISLpn7 is 79% (ORFA) and 82% (ORFB) aa similar to ISAma1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Cazalet,C., Rusniok,C., Br, Zidane,N., Magnier,A., Ma,L., Tichit,M., Jarraud,S., Bouchier,C., Vandenesch,F., Kunst,F., Etienne,J., Glaser,P. and Buchrieser,C. (2004) Nat. Genet. 36 (11), 1165-1173.