ISClsp1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAQV01000022 | ND | Clostridium sp. | Clostridium sp. OhILAs |
DNA section
IS Length : 1222 bp
Ends
IR Length : 25/41
IRL : TGGATAGGCTACAATTAATTGGACAGATAATTTAAGGGCATGTACAATAA
IRR : TGGATTGTCAACAGTTTTTCAGACAACTTTTTTAAGGGTATTCATTCTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTAAAGAAA | CCACATCAAT | 0 |
DNA sequence
TGGATAGGCTACAATTAATTGGACAGATAATTTAAGGGCATGTACAATAAAAATATAAAATTAAAGGAGCTACGCCCATGTCTGTTGAAAAACGTCCTCG
CCCTACCTATACGGATGAGTTTAAAAACCAAGTAGTACAATTATATAAGAATGGAAAACGCAAATGCAATATTACCCGCGAATACGATATTGCTTCTTCT
TTATTAGACAAATGGATCAAACAGGCGGATACTAGTGGTTCTTTCAAAGAAAAAGATAATCTTACTCTAGAACAAAAGGAACTAATTGAACTTCGTAAAC
GCAACAAACAGCTTGAAATGGAAAATGATATTTTAAAGCAAGCCGCGCTGATTATGGGACGAAAGTAAATGTGATTCGTAACAATGCTCACAAATACTCA
GTATCAGCAATGTGCAAGGTCCTAGAAATTTCAAGAAGTAGTTATTACTATGAATCAAAGCCAAAACAGTATGAATCTAAGCTAACTTCCAAAATCAAGA
AAATTTTTCAGGAAAGCCGTAGTAATTATGGCACCAGAAAAATCAAAGCAGCTCTTAGAGACAATGGATATCAAGTCTCCCGGCGCAGAACCGGAAGAAT
CATGAAGCAGGAAGGGTTAGTATCAAAGTATACAGTGGCTCAGTTTAGGCCATATGCAGATAAATGCAACGAATCACAAATAGAAAACTTGGTAAAACGG
AAATTCGATGATCGTTCACAATATAATGTAGTTGTAAGTGATTTAACATATGTACGAGTAGGAAATCAATGGAATTACATATGTGTTCTTGTCGACCTAT
TTAATCGTGAAATCATCGGATATAGTGCAGGTAAAAATAAAGACGCTAACCTAGTTTCAAAGGCATTTGCACGTGTAAATACAAATTTGAAAAATATCCA
GGTATTTCATACAGATAGAGGCAATGAATTCAAGAACAAACTTCTGGACGAAACCCTTAAAATATTTGATATAAAGCGCTCCCTTAGCAAGAGAGGATGT
CCTTATGATAATGCAGTTGCAGAAGCAACGTTTAAAATTATAAAGACGGAATTTGTAAAATATAAGGTATTTGAAACGCTAGAGGAATTACAATATGAAC
TGGCTGATTATGTGAACTGGTTTCATAATCACCGACTTCATTCCTCGTTAGGTTATTTAACGCCAGCTACATTCAGAATGAATACCCTTAAAAAAGTTGT
CTGAAAAACTGTTGACAATCCA
CCCTACCTATACGGATGAGTTTAAAAACCAAGTAGTACAATTATATAAGAATGGAAAACGCAAATGCAATATTACCCGCGAATACGATATTGCTTCTTCT
TTATTAGACAAATGGATCAAACAGGCGGATACTAGTGGTTCTTTCAAAGAAAAAGATAATCTTACTCTAGAACAAAAGGAACTAATTGAACTTCGTAAAC
GCAACAAACAGCTTGAAATGGAAAATGATATTTTAAAGCAAGCCGCGCTGATTATGGGACGAAAGTAAATGTGATTCGTAACAATGCTCACAAATACTCA
GTATCAGCAATGTGCAAGGTCCTAGAAATTTCAAGAAGTAGTTATTACTATGAATCAAAGCCAAAACAGTATGAATCTAAGCTAACTTCCAAAATCAAGA
AAATTTTTCAGGAAAGCCGTAGTAATTATGGCACCAGAAAAATCAAAGCAGCTCTTAGAGACAATGGATATCAAGTCTCCCGGCGCAGAACCGGAAGAAT
CATGAAGCAGGAAGGGTTAGTATCAAAGTATACAGTGGCTCAGTTTAGGCCATATGCAGATAAATGCAACGAATCACAAATAGAAAACTTGGTAAAACGG
AAATTCGATGATCGTTCACAATATAATGTAGTTGTAAGTGATTTAACATATGTACGAGTAGGAAATCAATGGAATTACATATGTGTTCTTGTCGACCTAT
TTAATCGTGAAATCATCGGATATAGTGCAGGTAAAAATAAAGACGCTAACCTAGTTTCAAAGGCATTTGCACGTGTAAATACAAATTTGAAAAATATCCA
GGTATTTCATACAGATAGAGGCAATGAATTCAAGAACAAACTTCTGGACGAAACCCTTAAAATATTTGATATAAAGCGCTCCCTTAGCAAGAGAGGATGT
CCTTATGATAATGCAGTTGCAGAAGCAACGTTTAAAATTATAAAGACGGAATTTGTAAAATATAAGGTATTTGAAACGCTAGAGGAATTACAATATGAAC
TGGCTGATTATGTGAACTGGTTTCATAATCACCGACTTCATTCCTCGTTAGGTTATTTAACGCCAGCTACATTCAGAATGAATACCCTTAAAAAAGTTGT
CTGAAAAACTGTTGACAATCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 78 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MSVEKRPRPTYTDEFKNQVVQLYKNGKRKCNITREYDIASSLLDKWIKQADTSGSFKEKDNLTLEQKELIELRKRNKQLEMENDILKQAALIMGRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 291 aa | 329 | 1204 | + | No |
Description : Second part of the transposase
ORF sequence :
YFKASRADYGTKVNVIRNNAHKYSVSAMCKVLEISRSSYYYESKPKQYESKLTSKIKKIFQESRSNYGTRKIKAALRDNGYQVSRRRTGRIMKQEGLVSK
YTVAQFRPYADKCNESQIENLVKRKFDDRSQYNVVVSDLTYVRVGNQWNYICVLVDLFNREIIGYSAGKNKDANLVSKAFARVNTNLKNIQVFHTDRGNE
FKNKLLDETLKIFDIKRSLSKRGCPYDNAVAEATFKIIKTEFVKYKVFETLEELQYELADYVNWFHNHRLHSSLGYLTPATFRMNTLKKVV
YTVAQFRPYADKCNESQIENLVKRKFDDRSQYNVVVSDLTYVRVGNQWNYICVLVDLFNREIIGYSAGKNKDANLVSKAFARVNTNLKNIQVFHTDRGNE
FKNKLLDETLKIFDIKRSLSKRGCPYDNAVAEATFKIIKTEFVKYKVFETLEELQYELADYVNWFHNHRLHSSLGYLTPATFRMNTLKKVV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1127 bp | 375 aa | 78 | 1204 | + | Yes |
Chemistry : DDE
ORF sequence :
MSVEKRPRPTYTDEFKNQVVQLYKNGKRKCNITREYDIASSLLDKWIKQADTSGSFKEKDNLTLEQKELIELRKRNKQLEMENDILKASRADYGTKVNVI
RNNAHKYSVSAMCKVLEISRSSYYYESKPKQYESKLTSKIKKIFQESRSNYGTRKIKAALRDNGYQVSRRRTGRIMKQEGLVSKYTVAQFRPYADKCNES
QIENLVKRKFDDRSQYNVVVSDLTYVRVGNQWNYICVLVDLFNREIIGYSAGKNKDANLVSKAFARVNTNLKNIQVFHTDRGNEFKNKLLDETLKIFDIK
RSLSKRGCPYDNAVAEATFKIIKTEFVKYKVFETLEELQYELADYVNWFHNHRLHSSLGYLTPATFRMNTLKKVV
RNNAHKYSVSAMCKVLEISRSSYYYESKPKQYESKLTSKIKKIFQESRSNYGTRKIKAALRDNGYQVSRRRTGRIMKQEGLVSKYTVAQFRPYADKCNES
QIENLVKRKFDDRSQYNVVVSDLTYVRVGNQWNYICVLVDLFNREIIGYSAGKNKDANLVSKAFARVNTNLKNIQVFHTDRGNEFKNKLLDETLKIFDIK
RSLSKRGCPYDNAVAEATFKIIKTEFVKYKVFETLEELQYELADYVNWFHNHRLHSSLGYLTPATFRMNTLKKVV
Blast result :
Comments
Genome in progress.
ISClsp1 is 80% (ORFA) and 70% (ORFB) aa similar to IS1485. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISClsp1 is 80% (ORFA) and 70% (ORFB) aa similar to IS1485. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.