ISfinder Mirror Site

ISMca7

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002977	ND	Methylococcus capsulatus	Methylococcus capsulatus Bath

DNA section

IS Length : 1172 bp

Ends

IR Length : 19

IRL : GGAACCTCTGAACAAACCCTGGTGATAAGCTAATCCCTGCCCAGAACCCC
IRR : GGAACCTCTGAACAAACCCGATTTGGCTGGCGAAAACACATCCCGCTGAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CACCTTGAACAAGC	TTAG	GTTGTATTTCTAGTCT	4

DNA sequence

GGAACCTCTGAACAAACCCTGGTGATAAGCTAATCCCTGCCCAGAACCCCTGAACGCGGAGCCCCCATGAAACAGACCACCTTCGCCTCCTTGGCCTTTG
ACCGGAAGAAGAAGCAGACCCGGCGCGAGAAGTTCCTGGCCGAGATGGAGCGGGCGGTGCCGTGGCCGGAACTGCTGGCGGTGATCGAACCGCATTACCC
GAAAGCGGGTCGGCGCGGTCGTCAACCGTACCCGTTGGCGACGATGCTCAGGCTTTACTTCCTCCAGCACTGGTATGCCTTGTCCGACCCGGGCCTGGAA
GATGCCCTGTACGAGATCGAATCGATGCGGCGTTTTGCGGGTCTGGAGTTGGCCGATGATGCGCTGCCGGATGAAACCACGATCCTCAACTTCCGGCGCC
TGCTGGAACGTCACGCGCTGACGGCGAAGCTGATGAACACGATCAATGACGTGCTGGAAGCGCGAGGGCTGCTGCTCAAGGGGGGCACGATGGTGGATGC
GACGATCATCCACGCCGCGCCATCGACGAAGAACAAAGCCAAGACCCGCGATCCCGAGATGCATCAGACCAAGAAGGGCAATCCGTGGTTCTTCGGCATG
AAGGTGCACGTGGGAGCGGATGTTCACACCGGTGTCGCCCACACGGTCTCGGTCACACCGGCCCACGCCTCGGACATCAGCCAGTTGCCGAACCTGCTGC
GCGAGGACGACCGGCTGATCCTCGGCGATGCCGGCTACGTCAACGACACCTACAAGCGCGCGGCGCGGCAGGCCGGGGTCGTGTGGGGCGTGGCCCTGAA
GGCCCGTCCGAAACGGCGGCTGGGGTCGGCGCAGAAACGCCGCAATCGAAAGCTGTCCTCGCTGCGCAGCCGCGTCGAGCACCTCTTTCGGGTGATGAAG
CGGCAGTTCGGCTACACCAAGACGCGCTACCGGGGCCTGGCCAAGAACGCCGCCCAGGTGTTTACCCTGATAGGGTTGACCCATCTCTACCTGAAGCGGT
ACGCATTGATGACCTGACGGGCGAGGTGCGTCCGGAATCCGCCCCGACGGGCGCATCCGGGCGGGAATCGCCACGGCAAGTAAGGAATTTCGGTACCCTT
CACCCATTTTCAAAGCCTGATTATCAGCGGGATGTGTTTTCGCCAGCCAAATCGGGTTTGTTCAGAGGTTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	67	1017	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKQTTFASLAFDRKKKQTRREKFLAEMERAVPWPELLAVIEPHYPKAGRRGRQPYPLATMLRLYFLQHWYALSDPGLEDALYEIESMRRFAGLELADDAL
PDETTILNFRRLLERHALTAKLMNTINDVLEARGLLLKGGTMVDATIIHAAPSTKNKAKTRDPEMHQTKKGNPWFFGMKVHVGADVHTGVAHTVSVTPAH
ASDISQLPNLLREDDRLILGDAGYVNDTYKRAARQAGVVWGVALKARPKRRLGSAQKRRNRKLSSLRSRVEHLFRVMKRQFGYTKTRYRGLAKNAAQVFT
LIGLTHLYLKRYALMT

Blast result :

Comments

ISMca7 is 71% aa similar to IS2000.

References

1] Ward,N., Larsen,O., Sakwa,J., Bruseth,L., Khouri,H., Durkin,A.S., Dimitrov,G., Jiang,L., Scanlan,D., Kang,K.H., Lewis,M., Nelson,K.E., Methe,B., Wu,M., Heidelberg,J.F., Paulsen,I.T., Fouts,D., Ravel,J., Tettelin,H., Ren,Q., Read,T., DeBoy,R.T., Seshadri,R., Salzberg,S.L., Jensen,H.B., Birkeland,N.K., Nelson,W.C., Dodson,R.J., Grindhaug,S.H., Holt,I., Eidhammer,I., Jonasen,I., Vanaken,S., Utterback,T., Feldblyum,T.V., Fraser,C.M., Lillehaug,J.R. and Eisen,J.A. (2004) PLoS Biol. 2(10):e303.