ISfinder Mirror Site

ISMca5

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002977	ND	Methylococcus capsulatus	Methylococcus capsulatus Bath

DNA section

IS Length : 1390 bp

Ends

IR Length : 22/29

IRL : GAGCCTGTCAGGAATTTTGTGTTTGAGGCATAGCATGTTGGAAAGGAGCA
IRR : GGGACTGTCTGAATTTTTGTGTTCAAGGCTCTTTATTCGGGCTGCCGTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATGAACGCCC	GCGTGCGA	AACCACATCG	8
GGTCTTGCGG	ATCGTCCG	AATCCGCCGC	8

DNA sequence

GAGCCTGTCAGGAATTTTGTGTTTGAGGCATAGCATGTTGGAAAGGAGCATCTATGCCAAGCAAGAAGAAACCGGCGGCAGCCGGCATGACGGCGCTGCC
GTCTATTCCCCACGAGCTCATTGAACAATTGACCGGCGGCGCCACGCCGATGACGGCCGAACAGATCAACGCCACCACCATGGCGCTGAAGAAAGCGCTG
ATCGAGCGTGCGCTGGGCGCAGAGCTGTCGTACCACCTGGGCTATCCGCCCGGCAGCGCCCGCCCCGAGGATGCAGCCAACCAGCGCAACGGCAAGTCCG
CCAAGACGGTGCTGACTCAGGACGGGCCGCTGCGCATCGAGGTGCCGCGCGACCGCGCAGGCTCCTTCGAGCCCATCCTGATCCCCAAGCACGAGCGGCG
TTTCACGGGCTTTGACGACAAGATCGTGGCCATGTACGCGCGGGGCATGACCGTGCGTGAGATCCAGGGCTTCCTGGCTGAGCAGTACGGCACCGAGGTC
AGTCCTGAATTCATCAGTTGTGTCACGGATGAGGTGATGGCCGAGGTCACCGCCTGGCAGGGCAGGCCGTTGGAGCCGATGTACCCGGTCGTATTCTTCG
ACTGTCTGCGGGTCAAGATCCGCGAGGATGCCGTGGTGCGCAACAAGGCCATCTATCTGGCGCTGGGCGTGCTGCCCGACGGTACGCGCGACATCCTGGG
CCTGTGGATCGAGAACACCGAAGGGGCCAAGTTCTGGATGAAGGTGTTCAACGATCTGAAGACGCGCGGCGTGGCCGACATCCTGATTGCCGTCACCGAC
GGGCTCAAGGGCATTCCCGAGGCGCTTGGGGCGGTGTTTCCGGCCACGACGCTGCAGACCTGTATCGTGCACTTGATCCGCAACAGCCTGGACTATGCCA
GCTGGAAGGACCGCAAAGCGCTGGCTGCGGCGCTCAAGCCCATCTACACGGCGCCCAGCGCAGAAGCCGCAGCGGTCGAACTCGATGCCCTGGAGGCTGG
CCCCTGGGGGCAGAAGTTCCCCACGGTGGTGGCGACCTGGCGCCGGGCCTGGGACCGCGTCATTCCGTTTTTTGCGTTCAGCCCGGCGGTGCGCAGAGTG
ATCTACACGACCAATGCCATCGAGAGCCTGCACAGTCAGCTGCGCAAGATCATCAAGACGCGGGGGCACTTTCCCAATGACGAGGCCGCCACCAAGCTGC
TGTGGCTGGCCTTGCGCAACATCACGGCTGACTGGGGCCGAGCAGCCAGGGAGTGGAAAGACGCCATGAACCAGTTCGCCATCGCCTAGGGGGAGCGCTT
TACACGACCGGCCGCGTAATATGTCCACTGGGCTCGTCTGAAACGGCAGCCCGAATAAAGAGCCTTGAACACAAAAATTCAGACAGTCCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1236 bp	411 aa	54	1289	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPSKKKPAAAGMTALPSIPHELIEQLTGGATPMTAEQINATTMALKKALIERALGAELSYHLGYPPGSARPEDAANQRNGKSAKTVLTQDGPLRIEVPRD
RAGSFEPILIPKHERRFTGFDDKIVAMYARGMTVREIQGFLAEQYGTEVSPEFISCVTDEVMAEVTAWQGRPLEPMYPVVFFDCLRVKIREDAVVRNKAI
YLALGVLPDGTRDILGLWIENTEGAKFWMKVFNDLKTRGVADILIAVTDGLKGIPEALGAVFPATTLQTCIVHLIRNSLDYASWKDRKALAAALKPIYTA
PSAEAAAVELDALEAGPWGQKFPTVVATWRRAWDRVIPFFAFSPAVRRVIYTTNAIESLHSQLRKIIKTRGHFPNDEAATKLLWLALRNITADWGRAARE
WKDAMNQFAIA

Blast result :

Comments

There are 2 copies of ISMca5 in this strain. ISMca5 is 91% aa similar to ISCsp1.

References

1] Ward,N., Larsen,O., Sakwa,J., Bruseth,L., Khouri,H., Durkin,A.S., Dimitrov,G., Jiang,L., Scanlan,D., Kang,K.H., Lewis,M., Nelson,K.E., Methe,B., Wu,M., Heidelberg,J.F., Paulsen,I.T., Fouts,D., Ravel,J., Tettelin,H., Ren,Q., Read,T., DeBoy,R.T., Seshadri,R., Salzberg,S.L., Jensen,H.B., Birkeland,N.K., Nelson,W.C., Dodson,R.J., Grindhaug,S.H., Holt,I., Eidhammer,I., Jonasen,I., Vanaken,S., Utterback,T., Feldblyum,T.V., Fraser,C.M., Lillehaug,J.R. and Eisen,J.A. (2004) PLoS Biol. 2(10):e303.