ISDha6
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007907 | ND | Desulfitobacterium hafniense | Desulfitobacterium hafniense Y51 |
DNA section
IS Length : 1333 bp
Ends
IR Length : 27/42
IRL : TGACTCATACCCGATTTGATGGACAAAATCCACTCGTATATAATAAAACC
IRR : TGACCTTTCCCCATAAAGATAGACACTTCTTACTCGTAAATGCTCGTTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTGAAAAAA | GTTGTTCTAG | 0 |
DNA sequence
TGACTCATACCCGATTTGATGGACAAAATCCACTCGTATATAATAAAACCAAGTAGAAGGAGTGGAGAAAATGAAGAAACGCTACTCAGAGGAATTTAAG
GTTGAAGCTGTCAGAATGGTACTTGAGGGAAATAGAAGCCAGCATCAAGTGGCGAATGATTTAGGATTATCAGTCACTACATTATCGGGATGGGTGTGTA
AATATAAATCGAACCCTGAGCGCGGGATTGCCGGATCATTGAATTTAACAGATGCAGAACGAGAGCTAAAAGCGCTAAAGAAGAAATATCGAGACCTTGA
AGAGGAAAATGAAATCCTAAAAAAAGCAGCAGCCTACTTCGCAAAGAATTTGAAATAACAGAGAGATTCTTATTCATCCAGGCTAATCGCTGTAATTACA
AGGTTGCGAAGATGTGTAGCGTCCTCCGTGTTTCAAAGTCGGGATACTACGCATGGATTAACCGTCATGACTCAAAGCGAGCTGAAGACAATAAACTACT
GCTTCAGGACATCAGGGATGTCTTCAATGCCTCTAATGGAGTGTATGGGAGCATTAAAGTTAAAAAGGCTATCGAAGCCAAGAAGCTGGAGGATCGCTAC
TCTAAACTTGCAAGAATTAATCATAAGCGTATAGAGCGTATCATGCGCAATGAAGGGATCCTGTCCAAGGTTCATAAGAAATACAAAGCGACGACGAATT
CCAATCACAACCTACCCGTTGCAGAAAACATTCTCAATCGTGAATTCGAGGCTAAGAGACCCAATGAGAAACTTGTCTCAGATATAACATACATCTCGAC
AGAAGAAGGCTGGCTGTATGTCGCTGGCATACAAGACTTATGCGGAGGAAAGATGGTTGGGTTATCCATGTCAGACAGGATGACGAAAGCTCTGGTGCTT
AATGCCTTACATGATGCCTATCGAACTGCGGGCAGGCCCCAAAACGCAATCCTGCACTCAGACAGAGGCAGTCAGTATTGTTCCTACGCTTACCAAGAAA
AACTCAAGGAATATGGTTATACTTGTAGTATGTCACGGAAAGGGAACTGTTGGGATAATGCACCGATGGAATCATTCTGGGGCAAGATGAAACAAGAATG
GATCAATGGTCAGAAATTCAAGACTAGGGAAGAAGCCAAAGCTAAGGTTTTTGAATATATTATGATCTTTTACAACAGAAAAAGATTACACGCCAGCTAT
GATTACAAGACACCAGATGCTTACTATAATGAGAAGAAAACAGGCAAAAGTGCTGCATAAAAAATTATTAGCATACATGCGAATTAACGAGCATTTACGA
GTAAGAAGTGTCTATCTTTATGGGGAAAGGTCA
GTTGAAGCTGTCAGAATGGTACTTGAGGGAAATAGAAGCCAGCATCAAGTGGCGAATGATTTAGGATTATCAGTCACTACATTATCGGGATGGGTGTGTA
AATATAAATCGAACCCTGAGCGCGGGATTGCCGGATCATTGAATTTAACAGATGCAGAACGAGAGCTAAAAGCGCTAAAGAAGAAATATCGAGACCTTGA
AGAGGAAAATGAAATCCTAAAAAAAGCAGCAGCCTACTTCGCAAAGAATTTGAAATAACAGAGAGATTCTTATTCATCCAGGCTAATCGCTGTAATTACA
AGGTTGCGAAGATGTGTAGCGTCCTCCGTGTTTCAAAGTCGGGATACTACGCATGGATTAACCGTCATGACTCAAAGCGAGCTGAAGACAATAAACTACT
GCTTCAGGACATCAGGGATGTCTTCAATGCCTCTAATGGAGTGTATGGGAGCATTAAAGTTAAAAAGGCTATCGAAGCCAAGAAGCTGGAGGATCGCTAC
TCTAAACTTGCAAGAATTAATCATAAGCGTATAGAGCGTATCATGCGCAATGAAGGGATCCTGTCCAAGGTTCATAAGAAATACAAAGCGACGACGAATT
CCAATCACAACCTACCCGTTGCAGAAAACATTCTCAATCGTGAATTCGAGGCTAAGAGACCCAATGAGAAACTTGTCTCAGATATAACATACATCTCGAC
AGAAGAAGGCTGGCTGTATGTCGCTGGCATACAAGACTTATGCGGAGGAAAGATGGTTGGGTTATCCATGTCAGACAGGATGACGAAAGCTCTGGTGCTT
AATGCCTTACATGATGCCTATCGAACTGCGGGCAGGCCCCAAAACGCAATCCTGCACTCAGACAGAGGCAGTCAGTATTGTTCCTACGCTTACCAAGAAA
AACTCAAGGAATATGGTTATACTTGTAGTATGTCACGGAAAGGGAACTGTTGGGATAATGCACCGATGGAATCATTCTGGGGCAAGATGAAACAAGAATG
GATCAATGGTCAGAAATTCAAGACTAGGGAAGAAGCCAAAGCTAAGGTTTTTGAATATATTATGATCTTTTACAACAGAAAAAGATTACACGCCAGCTAT
GATTACAAGACACCAGATGCTTACTATAATGAGAAGAAAACAGGCAAAAGTGCTGCATAAAAAATTATTAGCATACATGCGAATTAACGAGCATTTACGA
GTAAGAAGTGTCTATCTTTATGGGGAAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 65 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MEKMKKRYSEEFKVEAVRMVLEGNRSQHQVANDLGLSVTTLSGWVCKYKSNPERGIAGSLNLTDAERELKALKKKYRDLEEENEILKKAAAYFAKNLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 313 | 1260 | + | No |
Description : Second part of the transposase
ORF sequence :
NPKKSSSLLRKEFEITERFLFIQANRCNYKVAKMCSVLRVSKSGYYAWINRHDSKRAEDNKLLLQDIRDVFNASNGVYGSIKVKKAIEAKKLEDRYSKLA
RINHKRIERIMRNEGILSKVHKKYKATTNSNHNLPVAENILNREFEAKRPNEKLVSDITYISTEEGWLYVAGIQDLCGGKMVGLSMSDRMTKALVLNALH
DAYRTAGRPQNAILHSDRGSQYCSYAYQEKLKEYGYTCSMSRKGNCWDNAPMESFWGKMKQEWINGQKFKTREEAKAKVFEYIMIFYNRKRLHASYDYKT
PDAYYNEKKTGKSAA
RINHKRIERIMRNEGILSKVHKKYKATTNSNHNLPVAENILNREFEAKRPNEKLVSDITYISTEEGWLYVAGIQDLCGGKMVGLSMSDRMTKALVLNALH
DAYRTAGRPQNAILHSDRGSQYCSYAYQEKLKEYGYTCSMSRKGNCWDNAPMESFWGKMKQEWINGQKFKTREEAKAKVFEYIMIFYNRKRLHASYDYKT
PDAYYNEKKTGKSAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1196 bp | 399 aa | 65 | 1260 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKMKKRYSEEFKVEAVRMVLEGNRSQHQVANDLGLSVTTLSGWVCKYKSNPERGIAGSLNLTDAERELKALKKKYRDLEEENEILKKSSSLLRKEFEIT
ERFLFIQANRCNYKVAKMCSVLRVSKSGYYAWINRHDSKRAEDNKLLLQDIRDVFNASNGVYGSIKVKKAIEAKKLEDRYSKLARINHKRIERIMRNEGI
LSKVHKKYKATTNSNHNLPVAENILNREFEAKRPNEKLVSDITYISTEEGWLYVAGIQDLCGGKMVGLSMSDRMTKALVLNALHDAYRTAGRPQNAILHS
DRGSQYCSYAYQEKLKEYGYTCSMSRKGNCWDNAPMESFWGKMKQEWINGQKFKTREEAKAKVFEYIMIFYNRKRLHASYDYKTPDAYYNEKKTGKSAA
ERFLFIQANRCNYKVAKMCSVLRVSKSGYYAWINRHDSKRAEDNKLLLQDIRDVFNASNGVYGSIKVKKAIEAKKLEDRYSKLARINHKRIERIMRNEGI
LSKVHKKYKATTNSNHNLPVAENILNREFEAKRPNEKLVSDITYISTEEGWLYVAGIQDLCGGKMVGLSMSDRMTKALVLNALHDAYRTAGRPQNAILHS
DRGSQYCSYAYQEKLKEYGYTCSMSRKGNCWDNAPMESFWGKMKQEWINGQKFKTREEAKAKVFEYIMIFYNRKRLHASYDYKTPDAYYNEKKTGKSAA
Blast result :
Comments
ISDha6 is 62 % (ORFA) and 62 % (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Nonaka,H., Keresztes,G., Shinoda,Y., Ikenaga,Y., Abe,M., Naito,K., Inatomi,K., Furukawa,K., Inui,M. and Yukawa,H. (2006) Bacteriol. 188 (6), 2262-2274.