ISGlo1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAVG01000024 | ND | Geobacter lovleyi | Geobacter lovleyi SZ |
DNA section
IS Length : 1259 bp
Ends
IR Length : 24/31
IRL : TGACGTATCCTGATGACAACAGACACTACCACTTGATAAATTACCGAGTG
IRR : TGACGTGTCCTGTCAACAGTAGACACTCCCAACTCAAGCGACTTGCTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATCACCGAT | TTGATCACTG | 0 |
DNA sequence
TGACGTATCCTGATGACAACAGACACTACCACTTGATAAATTACCGAGTGGGAGGAAGTCATGGGCAAGCAGCAACGTTACACCCCGGAATTTCGGTCTG
AAGCAGTTAAACTCGTATTAGAGCAAAATCTGTCGCTTTCAGTAGCATCCAAGCGACTGGGTATCCCAGAAGGAACGTTGGCCAGCTGGATGGCAGCTGT
TAGATCTTCTTCAAAACCATCTGCTCCAGGGTCACGTTCAACACCTGATCTCTTGGCAGAGAATGCCAGGTTGAGGAAGGAGCTGGCAGAAACCCGCATG
GAGCGTGATATCTTAAAAAAGGCAACGGCGTACTTTGCCAGGGAGTCGCTACCCGGTACGCGTTCATGAAAAAATGGCGACTCCACTATCCTACTGTAAC
TATGAGCCGTATGTTTAATGTGTCCCGTAGCGGCTTTTATGCGTGGTTAAAAAGAAACCCGTCACGGCGGACTCAGGAGGATGAGCGCCTCAAGGTTGCA
ATCAAGGTAGCGCATACACGTACTCGTGAGAGTTACAGCGCGAAACGGTTACAACCTGAACTGGCAGCAGATGGCTTTATTGTCGGTCGTGATCGCATCG
CTCGCTTACGACGAGAATTGGGATTACGCTGCCGGCAGAAGCGCAAGTTTAAAGCAACTACGAACTCAAACCACAATCTTCCTGTGGCAGAGAACCTTCT
TGATCAAACTTTTACACCAAGCGCACCAAACGAAGTATGGGTTACCGATATCACCTACATCCCAACAGGAGAAGGCTGGCTTTACCTTGCTGGAGTTAAG
GATGTTTTTACACGCGAAATAGTCGGTTACGCCATGTCAAAACGAATGACGCAGGAGTTGACAGGCCGGGCTCTCTTTCGAGCGGTACAACAAAAACGAC
CTGCTGCCGGGTTGATTCATCATTCTGATCGCGGCAGTCAATACTGCGCTCACGATTATAGAAAGCTTCTGAAGCAGTTCAAAATGAAGACATCCATGTC
ACGCAAAGGAAATTGCTATGATAATGCGCCGATGGAAAGCTTCTGGGGCAGCCTCAAAAACGAACTTGTACATCATTGCCGCTATGAAACCAGAGATGAA
GCAAAAGCTTCTATCCAGGAATACATTGAGATCTTTTATAACCGCCAAAGACGCCATTCCCGTCTTGGATATGTTGCACCGGCAATATTTGCACAGAATT
ACAACAATCAGCAGCAAGTCGCTTGAGTTGGGAGTGTCTACTGTTGACAGGACACGTCA
AAGCAGTTAAACTCGTATTAGAGCAAAATCTGTCGCTTTCAGTAGCATCCAAGCGACTGGGTATCCCAGAAGGAACGTTGGCCAGCTGGATGGCAGCTGT
TAGATCTTCTTCAAAACCATCTGCTCCAGGGTCACGTTCAACACCTGATCTCTTGGCAGAGAATGCCAGGTTGAGGAAGGAGCTGGCAGAAACCCGCATG
GAGCGTGATATCTTAAAAAAGGCAACGGCGTACTTTGCCAGGGAGTCGCTACCCGGTACGCGTTCATGAAAAAATGGCGACTCCACTATCCTACTGTAAC
TATGAGCCGTATGTTTAATGTGTCCCGTAGCGGCTTTTATGCGTGGTTAAAAAGAAACCCGTCACGGCGGACTCAGGAGGATGAGCGCCTCAAGGTTGCA
ATCAAGGTAGCGCATACACGTACTCGTGAGAGTTACAGCGCGAAACGGTTACAACCTGAACTGGCAGCAGATGGCTTTATTGTCGGTCGTGATCGCATCG
CTCGCTTACGACGAGAATTGGGATTACGCTGCCGGCAGAAGCGCAAGTTTAAAGCAACTACGAACTCAAACCACAATCTTCCTGTGGCAGAGAACCTTCT
TGATCAAACTTTTACACCAAGCGCACCAAACGAAGTATGGGTTACCGATATCACCTACATCCCAACAGGAGAAGGCTGGCTTTACCTTGCTGGAGTTAAG
GATGTTTTTACACGCGAAATAGTCGGTTACGCCATGTCAAAACGAATGACGCAGGAGTTGACAGGCCGGGCTCTCTTTCGAGCGGTACAACAAAAACGAC
CTGCTGCCGGGTTGATTCATCATTCTGATCGCGGCAGTCAATACTGCGCTCACGATTATAGAAAGCTTCTGAAGCAGTTCAAAATGAAGACATCCATGTC
ACGCAAAGGAAATTGCTATGATAATGCGCCGATGGAAAGCTTCTGGGGCAGCCTCAAAAACGAACTTGTACATCATTGCCGCTATGAAACCAGAGATGAA
GCAAAAGCTTCTATCCAGGAATACATTGAGATCTTTTATAACCGCCAAAGACGCCATTCCCGTCTTGGATATGTTGCACCGGCAATATTTGCACAGAATT
ACAACAATCAGCAGCAAGTCGCTTGAGTTGGGAGTGTCTACTGTTGACAGGACACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 61 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MGKQQRYTPEFRSEAVKLVLEQNLSLSVASKRLGIPEGTLASWMAAVRSSSKPSAPGSRSTPDLLAENARLRKELAETRMERDILKKATAYFARESLPGT
RS
RS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 308 | 1225 | + | No |
Description : Second part of the transposase
ORF sequence :
YLKKGNGVLCQGVATRYAFMKKWRLHYPTVTMSRMFNVSRSGFYAWLKRNPSRRTQEDERLKVAIKVAHTRTRESYSAKRLQPELAADGFIVGRDRIARL
RRELGLRCRQKRKFKATTNSNHNLPVAENLLDQTFTPSAPNEVWVTDITYIPTGEGWLYLAGVKDVFTREIVGYAMSKRMTQELTGRALFRAVQQKRPAA
GLIHHSDRGSQYCAHDYRKLLKQFKMKTSMSRKGNCYDNAPMESFWGSLKNELVHHCRYETRDEAKASIQEYIEIFYNRQRRHSRLGYVAPAIFAQNYNN
QQQVA
RRELGLRCRQKRKFKATTNSNHNLPVAENLLDQTFTPSAPNEVWVTDITYIPTGEGWLYLAGVKDVFTREIVGYAMSKRMTQELTGRALFRAVQQKRPAA
GLIHHSDRGSQYCAHDYRKLLKQFKMKTSMSRKGNCYDNAPMESFWGSLKNELVHHCRYETRDEAKASIQEYIEIFYNRQRRHSRLGYVAPAIFAQNYNN
QQQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1165 bp | 388 aa | 61 | 1225 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKQQRYTPEFRSEAVKLVLEQNLSLSVASKRLGIPEGTLASWMAAVRSSSKPSAPGSRSTPDLLAENARLRKELAETRMERDILKKGNGVLCQGVATRY
AFMKKWRLHYPTVTMSRMFNVSRSGFYAWLKRNPSRRTQEDERLKVAIKVAHTRTRESYSAKRLQPELAADGFIVGRDRIARLRRELGLRCRQKRKFKAT
TNSNHNLPVAENLLDQTFTPSAPNEVWVTDITYIPTGEGWLYLAGVKDVFTREIVGYAMSKRMTQELTGRALFRAVQQKRPAAGLIHHSDRGSQYCAHDY
RKLLKQFKMKTSMSRKGNCYDNAPMESFWGSLKNELVHHCRYETRDEAKASIQEYIEIFYNRQRRHSRLGYVAPAIFAQNYNNQQQVA
AFMKKWRLHYPTVTMSRMFNVSRSGFYAWLKRNPSRRTQEDERLKVAIKVAHTRTRESYSAKRLQPELAADGFIVGRDRIARLRRELGLRCRQKRKFKAT
TNSNHNLPVAENLLDQTFTPSAPNEVWVTDITYIPTGEGWLYLAGVKDVFTREIVGYAMSKRMTQELTGRALFRAVQQKRPAAGLIHHSDRGSQYCAHDY
RKLLKQFKMKTSMSRKGNCYDNAPMESFWGSLKNELVHHCRYETRDEAKASIQEYIEIFYNRQRRHSRLGYVAPAIFAQNYNNQQQVA
Blast result :
Comments
Genome in progress.
ISGlo1 is 69% (ORFA) and 82% (ORFB) aa similar to IS ISSd1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISGlo1 is 69% (ORFA) and 82% (ORFB) aa similar to IS ISSd1. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.