ISHor1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Halothermothrix orenii | Halothermothrix orenii H 168 |
DNA section
IS Length : 1275 bp
Ends
IR Length : 22/48
IRL : TGGGTTTAGGTATATAATTAACCCAGGAGGTGCCCATAATGGGAAGGAAA
IRR : TGGGGTTCCCCCAATAAATTAGACACATAGTTTAGACGTTTTTCTTTCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGGGACATT | TGCCAATCCT | 0 |
DNA sequence
TGGGTTTAGGTATATAATTAACCCAGGAGGTGCCCATAATGGGAAGGAAAAAATATTCAGAAGAATTTAAAAGAGAAGCTGTGGAACTTAGTTTTAGCTC
GGATAAATCTTGCACAGTTATAGCAAAAGAACTAGGTATAAGTTATCATAATCTAATCAGATGGCGTAGGGAATACAAAAACAAAGGTGACTTAGCTTTC
CCAGGCAATGGTAAACAAAAGTTAACCCCTGAACAGGAAGAAATTCAAAGACTAAAAAAAGAATTAAATGATATAAAAATAGAGCGTGATATATTAAAAA
AAGCAGTGCGCATCTTCTCAAAGGAACCGAAATGATATATGGTTTCATCCGGGATCACAGTAATGAATTTGCTGTGGAGAAGATGTGCCAGGTTTTAAAT
GTATCCCGGTCAGGATATTACAGCTGGTTAAATAGAAAACCTGGTAAAAGAGAGTTAACAAATAAAAAGTTAAAGTTGAAGATAGCTGAAATATACTGGC
AGACCAGGGGAAGGTGTGGTAGCCCCCGGATACATAAACAGCTTAAAAAAGAGGGTTATCATTATAATATTAAGCGTATTGAACGACTAATGAGGATAAT
GGGATTAAAAGCAATACAGAAAAGAAAGTTTAAAAGGACAACTAATTCTGACAATAATCTCCCTTTAAAAAATAATCTTCTCAAACGTAATTTTAGCGTT
ACCCAACCTGATAAAGTCTGGGTATCAGACATAACATATATTCCAACAAAAGAAGGCTGGCTTTATCTTTCAGTAGTGATTGACCTCTATTCTCGCAAGG
TTGTTGGTTGGTCAATGGACAATCATATGACAAAAAGGTTAGTGATTGATGCATTGGAAATGGCCGTACTAAATCGTAATCCCTCCAGGGGATTAATTTT
TCATTCTGACAGAGGTAGTCAGTATGCCAGCCATGATTTTCAAAAAACACTGTGGAAATACGGTATTATTTCATCAATGAGCCGCAAAGGTGATTGCTGG
GACAATGCAGTAGCTGAAAGTTTTTTTGCAACACTAAAAACAGAATTGATTTTCCACAACAAGTATCAGAGCAGGGCTCAGGCAAAACGTGATATATTTG
AGTATATAGAGATTTTTTATAATAGGATTCGATTACATTCTTCACTAGATTATAAAAGCCCGGAAGATTACGAGAAAGAAAGAAAAACGTCTAAACTATG
TGTCTAATTTATTGGGGGAACCCCA
GGATAAATCTTGCACAGTTATAGCAAAAGAACTAGGTATAAGTTATCATAATCTAATCAGATGGCGTAGGGAATACAAAAACAAAGGTGACTTAGCTTTC
CCAGGCAATGGTAAACAAAAGTTAACCCCTGAACAGGAAGAAATTCAAAGACTAAAAAAAGAATTAAATGATATAAAAATAGAGCGTGATATATTAAAAA
AAGCAGTGCGCATCTTCTCAAAGGAACCGAAATGATATATGGTTTCATCCGGGATCACAGTAATGAATTTGCTGTGGAGAAGATGTGCCAGGTTTTAAAT
GTATCCCGGTCAGGATATTACAGCTGGTTAAATAGAAAACCTGGTAAAAGAGAGTTAACAAATAAAAAGTTAAAGTTGAAGATAGCTGAAATATACTGGC
AGACCAGGGGAAGGTGTGGTAGCCCCCGGATACATAAACAGCTTAAAAAAGAGGGTTATCATTATAATATTAAGCGTATTGAACGACTAATGAGGATAAT
GGGATTAAAAGCAATACAGAAAAGAAAGTTTAAAAGGACAACTAATTCTGACAATAATCTCCCTTTAAAAAATAATCTTCTCAAACGTAATTTTAGCGTT
ACCCAACCTGATAAAGTCTGGGTATCAGACATAACATATATTCCAACAAAAGAAGGCTGGCTTTATCTTTCAGTAGTGATTGACCTCTATTCTCGCAAGG
TTGTTGGTTGGTCAATGGACAATCATATGACAAAAAGGTTAGTGATTGATGCATTGGAAATGGCCGTACTAAATCGTAATCCCTCCAGGGGATTAATTTT
TCATTCTGACAGAGGTAGTCAGTATGCCAGCCATGATTTTCAAAAAACACTGTGGAAATACGGTATTATTTCATCAATGAGCCGCAAAGGTGATTGCTGG
GACAATGCAGTAGCTGAAAGTTTTTTTGCAACACTAAAAACAGAATTGATTTTCCACAACAAGTATCAGAGCAGGGCTCAGGCAAAACGTGATATATTTG
AGTATATAGAGATTTTTTATAATAGGATTCGATTACATTCTTCACTAGATTATAAAAGCCCGGAAGATTACGAGAAAGAAAGAAAAACGTCTAAACTATG
TGTCTAATTTATTGGGGGAACCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 65 | 361 | + | No |
Description : First part of the transposase
ORF sequence :
MGRKKYSEEFKREAVELSFSSDKSCTVIAKELGISYHNLIRWRREYKNKGDLAFPGNGKQKLTPEQEEIQRLKKELNDIKIERDILKKAVRIFSKEPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 316 | 1233 | + | No |
Description : Second part of the transposase
ORF sequence :
YIKKSSAHLLKGTEMIYGFIRDHSNEFAVEKMCQVLNVSRSGYYSWLNRKPGKRELTNKKLKLKIAEIYWQTRGRCGSPRIHKQLKKEGYHYNIKRIERL
MRIMGLKAIQKRKFKRTTNSDNNLPLKNNLLKRNFSVTQPDKVWVSDITYIPTKEGWLYLSVVIDLYSRKVVGWSMDNHMTKRLVIDALEMAVLNRNPSR
GLIFHSDRGSQYASHDFQKTLWKYGIISSMSRKGDCWDNAVAESFFATLKTELIFHNKYQSRAQAKRDIFEYIEIFYNRIRLHSSLDYKSPEDYEKERKT
SKLCV
MRIMGLKAIQKRKFKRTTNSDNNLPLKNNLLKRNFSVTQPDKVWVSDITYIPTKEGWLYLSVVIDLYSRKVVGWSMDNHMTKRLVIDALEMAVLNRNPSR
GLIFHSDRGSQYASHDFQKTLWKYGIISSMSRKGDCWDNAVAESFFATLKTELIFHNKYQSRAQAKRDIFEYIEIFYNRIRLHSSLDYKSPEDYEKERKT
SKLCV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 399 aa | 65 | 1233 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRKKYSEEFKREAVELSFSSDKSCTVIAKELGISYHNLIRWRREYKNKGDLAFPGNGKQKLTPEQEEIQRLKKELNDIKIERDILKKSSAHLLKGTEMI
YGFIRDHSNEFAVEKMCQVLNVSRSGYYSWLNRKPGKRELTNKKLKLKIAEIYWQTRGRCGSPRIHKQLKKEGYHYNIKRIERLMRIMGLKAIQKRKFKR
TTNSDNNLPLKNNLLKRNFSVTQPDKVWVSDITYIPTKEGWLYLSVVIDLYSRKVVGWSMDNHMTKRLVIDALEMAVLNRNPSRGLIFHSDRGSQYASHD
FQKTLWKYGIISSMSRKGDCWDNAVAESFFATLKTELIFHNKYQSRAQAKRDIFEYIEIFYNRIRLHSSLDYKSPEDYEKERKTSKLCV
YGFIRDHSNEFAVEKMCQVLNVSRSGYYSWLNRKPGKRELTNKKLKLKIAEIYWQTRGRCGSPRIHKQLKKEGYHYNIKRIERLMRIMGLKAIQKRKFKR
TTNSDNNLPLKNNLLKRNFSVTQPDKVWVSDITYIPTKEGWLYLSVVIDLYSRKVVGWSMDNHMTKRLVIDALEMAVLNRNPSRGLIFHSDRGSQYASHD
FQKTLWKYGIISSMSRKGDCWDNAVAESFFATLKTELIFHNKYQSRAQAKRDIFEYIEIFYNRIRLHSSLDYKSPEDYEKERKTSKLCV
Blast result :
Comments
Genome in progress : NZ_AAOZ01000021.
ISHor1 is 67 % (ORFA) aa similar to ISAma1 and 72% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISHor1 is 67 % (ORFA) aa similar to ISAma1 and 72% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina , del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.