ISLca1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008526 | ND | Lactobacillus casei | Lactobacillus casei ATCC 334 |
DNA section
IS Length : 1613 bp
Ends
IR Length : 29/47
IRL : TGGGGCGTGCGGACAAATTCCTGGACTTAGGGTATGATGTCATTATGTAT
IRR : TGGGCGTGCGGACGTTTTTCTGGACTTTCACCATCAAGCTACCTTGCTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAATGGTGAA | TGCTGTGAGC | 0 |
DNA sequence
TGGGGCGTGCGGACAAATTCCTGGACTTAGGGTATGATGTCATTATGTATTCAAAAGCAGCTAAAAAACGAGCAATCGAGCTCTTCTACCAATATCATCG
TTCATGGAGTGCAGTAGTCAGAGAATTAGGCTATCCGTCTTTAGGCGCATTAAAACGATGGATTATAGATTACGAACAAGATGCCAAAAATGCGCTTGAT
GAGTCCCCACGAAAGAGAAAATCAAAGTACTCTGATGTGCAACGACAAACTGCAGTGGATCACTTCTTTGAACATGGGCAATTCATCACAAAAACGGTCA
GAGCGCTAGGTTATCCATCACGACAGCTTCTTCGTGAGTGGCTATTAACAGATCCTCGTTATGATATTTCACTGCGTAGCCGTACTGTTATGGTTAAGCC
TGCTACGCCGGCTGACGTCAAGGCTAAGGCTGTGGAGGCACTCTATAAACGAGAAGTCCCTGCCAGAAAAATTGCGGAAAATTTCGGGGTTAGCCGTGAA
ACACTTTACGCCTGGAAGCGAGATGCACTCGGTGAAAATTTCCCGGCCAAGCAGACGCCTAGTTTATCAAATGAAGAAAAAAATCAAGCAAACATCACCC
CCGAAGTTTCAGATCTCCAAGTCCGCATCACAGAGCTTGAACTTCAGAATGATATTCTTCTTCAAGTAAACGCCCTCCTAAAAAAAGATCTGGGCATCAA
CCAACTCAATCTAACTAATCGAGAAAAGGTTCAGGTGATTGATGCCCTGCGTGATAGATATTCGCTTAATACGCTGCGTAATGCCCTCCGATTATCAAAG
AGTAGCTATTTCTATCAACGTGACGCCATCGCCAAGGGCGATAAGTACAAAGCAGTACGTCCACGCTTGAAGATTATTTTTGAGCAGAACTACAAGAGCT
ATGGGTATCGCCGAATGAAGATGGAACTCGACGATGAGGGGATCCAACTCTCTGAAAAAGTGATCCGCCGGTTAATGGCTGAGGAAGGATTGAAAGTCAC
CATCACACGCTGTCGAAAATACAGCTCGTACGCGGGTGAAATAACGCCAGCCGTTGCGAATATCCTTGACCGAGATTTTCAATCAGAAGTCCCAAATCAA
AAGCTAGTCACAGATATTACAGAGTTTTCTATCCAGGCCGGGAAAGTATATCTATCACCACTCATTGATTGTTTTGATGGCCAAATTACGAGTTGGACCA
TTGGCACTAGCCCAAACGCTGAACTTGTCAACACAATGCTTAGCACAGCGGCCAAAGTATTGCCCGCCAGTGAAAAAACCATCGTTCATTCCGACAGAGG
CGCTCACTACCGCTGGTCAGGCTGGATTGAACTGATGGACGAGTTTGGCTTTACACGATCGATGTCGAAGAAAGGTAGCACTCCAGACAATGCTCAATCG
GAGAGCTTCTTTGGCCATCTCAAGACAGAGTTCTTTTACAATCGAAGCTGGCTTGGAAAGTCAATTGATGATTTCACACAAGCACTCAATGAGTATATTG
AGTGGTATAACACCAAACGAAGAAAAAAAGTTTTAGGAGGAAAGAGCCCGCAGGAATACCGTCAGAGCAAGGTAGCTTGATGGTGAAAGTCCAGAAAAAC
GTCCGCACGCCCA
TTCATGGAGTGCAGTAGTCAGAGAATTAGGCTATCCGTCTTTAGGCGCATTAAAACGATGGATTATAGATTACGAACAAGATGCCAAAAATGCGCTTGAT
GAGTCCCCACGAAAGAGAAAATCAAAGTACTCTGATGTGCAACGACAAACTGCAGTGGATCACTTCTTTGAACATGGGCAATTCATCACAAAAACGGTCA
GAGCGCTAGGTTATCCATCACGACAGCTTCTTCGTGAGTGGCTATTAACAGATCCTCGTTATGATATTTCACTGCGTAGCCGTACTGTTATGGTTAAGCC
TGCTACGCCGGCTGACGTCAAGGCTAAGGCTGTGGAGGCACTCTATAAACGAGAAGTCCCTGCCAGAAAAATTGCGGAAAATTTCGGGGTTAGCCGTGAA
ACACTTTACGCCTGGAAGCGAGATGCACTCGGTGAAAATTTCCCGGCCAAGCAGACGCCTAGTTTATCAAATGAAGAAAAAAATCAAGCAAACATCACCC
CCGAAGTTTCAGATCTCCAAGTCCGCATCACAGAGCTTGAACTTCAGAATGATATTCTTCTTCAAGTAAACGCCCTCCTAAAAAAAGATCTGGGCATCAA
CCAACTCAATCTAACTAATCGAGAAAAGGTTCAGGTGATTGATGCCCTGCGTGATAGATATTCGCTTAATACGCTGCGTAATGCCCTCCGATTATCAAAG
AGTAGCTATTTCTATCAACGTGACGCCATCGCCAAGGGCGATAAGTACAAAGCAGTACGTCCACGCTTGAAGATTATTTTTGAGCAGAACTACAAGAGCT
ATGGGTATCGCCGAATGAAGATGGAACTCGACGATGAGGGGATCCAACTCTCTGAAAAAGTGATCCGCCGGTTAATGGCTGAGGAAGGATTGAAAGTCAC
CATCACACGCTGTCGAAAATACAGCTCGTACGCGGGTGAAATAACGCCAGCCGTTGCGAATATCCTTGACCGAGATTTTCAATCAGAAGTCCCAAATCAA
AAGCTAGTCACAGATATTACAGAGTTTTCTATCCAGGCCGGGAAAGTATATCTATCACCACTCATTGATTGTTTTGATGGCCAAATTACGAGTTGGACCA
TTGGCACTAGCCCAAACGCTGAACTTGTCAACACAATGCTTAGCACAGCGGCCAAAGTATTGCCCGCCAGTGAAAAAACCATCGTTCATTCCGACAGAGG
CGCTCACTACCGCTGGTCAGGCTGGATTGAACTGATGGACGAGTTTGGCTTTACACGATCGATGTCGAAGAAAGGTAGCACTCCAGACAATGCTCAATCG
GAGAGCTTCTTTGGCCATCTCAAGACAGAGTTCTTTTACAATCGAAGCTGGCTTGGAAAGTCAATTGATGATTTCACACAAGCACTCAATGAGTATATTG
AGTGGTATAACACCAAACGAAGAAAAAAAGTTTTAGGAGGAAAGAGCCCGCAGGAATACCGTCAGAGCAAGGTAGCTTGATGGTGAAAGTCCAGAAAAAC
GTCCGCACGCCCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1536 bp | 511 aa | 45 | 1580 | + | No |
Chemistry : DDE
ORF sequence :
MYSKAAKKRAIELFYQYHRSWSAVVRELGYPSLGALKRWIIDYEQDAKNALDESPRKRKSKYSDVQRQTAVDHFFEHGQFITKTVRALGYPSRQLLREWL
LTDPRYDISLRSRTVMVKPATPADVKAKAVEALYKREVPARKIAENFGVSRETLYAWKRDALGENFPAKQTPSLSNEEKNQANITPEVSDLQVRITELEL
QNDILLQVNALLKKDLGINQLNLTNREKVQVIDALRDRYSLNTLRNALRLSKSSYFYQRDAIAKGDKYKAVRPRLKIIFEQNYKSYGYRRMKMELDDEGI
QLSEKVIRRLMAEEGLKVTITRCRKYSSYAGEITPAVANILDRDFQSEVPNQKLVTDITEFSIQAGKVYLSPLIDCFDGQITSWTIGTSPNAELVNTMLS
TAAKVLPASEKTIVHSDRGAHYRWSGWIELMDEFGFTRSMSKKGSTPDNAQSESFFGHLKTEFFYNRSWLGKSIDDFTQALNEYIEWYNTKRRKKVLGGK
SPQEYRQSKVA
LTDPRYDISLRSRTVMVKPATPADVKAKAVEALYKREVPARKIAENFGVSRETLYAWKRDALGENFPAKQTPSLSNEEKNQANITPEVSDLQVRITELEL
QNDILLQVNALLKKDLGINQLNLTNREKVQVIDALRDRYSLNTLRNALRLSKSSYFYQRDAIAKGDKYKAVRPRLKIIFEQNYKSYGYRRMKMELDDEGI
QLSEKVIRRLMAEEGLKVTITRCRKYSSYAGEITPAVANILDRDFQSEVPNQKLVTDITEFSIQAGKVYLSPLIDCFDGQITSWTIGTSPNAELVNTMLS
TAAKVLPASEKTIVHSDRGAHYRWSGWIELMDEFGFTRSMSKKGSTPDNAQSESFFGHLKTEFFYNRSWLGKSIDDFTQALNEYIEWYNTKRRKKVLGGK
SPQEYRQSKVA
Blast result :
Comments
ISLca1 is 69% (ORFAB) similar to IS1353. There is no -1 programmed frameshift.
References
1] Makarova,K., Slesarev,A., Wolf,Y., Sorokin,A., Mirkin,B., Koonin,E., Pavolv,A., Pavlova,N., Karamychev,V., Polouchine,N., Shakhova,V., Grigoriev,I., Lou,Y., Rohksar,D., Lucas,S., Huang,K., Goldstein,D.M., Hawkins,T., Plengvidhya,V., Welker,D., Hughes,J.,Goh,Y., Benson,A., Baldwin,K., Lee,J.-H., Diaz-Muniz,I., Dosti,B., Smeianov,V., Wechter,W., Barabote,R., Lorca,G., Altermann,E., Barrangou,R., Ganesan,B., Xie,Y., Rawsthorne,H., Tamir,D.,Parker,C., Breidt,F., Broadbent,J., Hutkins,R., O'Sulllivan,D., Steele,J., Unlu,G., Saier,M., Klaenhammer,T., Richardson,P., Kozyavkin,S., Weimer,B. and Mills,D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15611-15616.