ISFsp8
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAII01000004 | ND | Frankia sp. | Frankia sp. EAN1pec |
DNA section
IS Length : 1297 bp
Ends
IR Length : 28/44
IRL : TGAGGTGGGCCCCGTTCTTCGGACAGTGATCCCTACGAGGGATAATTGAT
IRR : TGAGGTGCTGCCCTTTTCTCGGACACGGACGTAGACGGACTATAGCAGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATCTCGATCTCG | GTCCTCTCCACGA | 0 |
DNA sequence
TGAGGTGGGCCCCGTTCTTCGGACAGTGATCCCTACGAGGGATAATTGATCCGTGAACGGAGGAGATCAATGAGTCAGTCACGCAGGCGATACACACCCG
AGTACAAAGACGAGGCGGTGAAGCTCGTCATCGATTCGGGCCGCCCCACATCGGCGGTCGCGAAAGATCTCGGTATCAACGAGGGAACGTTGGGGAGCTG
GGTCGCGACCTGGCGGCGGGCACACCAAAACGAAGAAGAACCGCTGTCGATGTCCGAACGGGCCCGGCTCCGCGAGCTGGAGAAGGAGAACCGGGAACTC
CGGATGGAGCGCGAGTTCCTCTCAAAAGCGACCGCCTTCTTCGCGCAACGCCATCAGTGACCGACAAGTACGAACTGATCGCCGCGGAGAAGGACAACTA
CCCGGTCACCAAGATGTGCCAGTGGCTCGTCGTGTCGACATCCGGTTTCTACGACTGGCACCGCAGGCCCGCGTCGACCCGGACCCGTCGGCACACCACC
CTCGACACCCACGTCCGGGCGGTGTTCGCCGCCTCCCGCCAGACCTACGGCGCCCGGCGGATCGCCGCCGCCCTGACCGCCTCCGGCCTGCGCGTCTCCG
TCCGCCTCGCCCGACGGATCATGCGCCGCGCAGGGCTGGAAGCCTGCCAGCCCAGGGCCTACCGCCGCACCACCATCCCCGGCCAGGCCCCGGCGCCCGC
CGACCACGTCCGCCGCGACTTCACCGCCACCCGCCCCGGCGAGAAGCTCATCGGGGACATCACCTATATCCGCACCGGCGAAGGATGGCTCTACCTCGCC
ACCGTCATCGACTGCTACTCCCGTAAAGTCGCCGGCTGGTCGATGGCCACCCACCTGCGCACCGAGCTCATCATCGACGCGTTCACCATGGCCGCCTCCC
GCACAACCCTCGCACCGAACGCGCTTTTCCACTCCGATCGTGGCGCCCAATACACGTCGGATGCCTACCATCGCGTCCTGAAACGCCATGGAGTACGCCC
GTCGGTCGGCGCCACCGGAGTCTGCTGGGACAATTCCGCTGCAGAGTCGTTCTTCGGAACGCTCAAGAACGAACTCGTGTACCGGGAAAACTACCCAACC
CGCCGCGCCGCACGTACCGCGATCGCCGAATACATCGAGGTCTTCTACAACCGGCAGCGGCTCCACTCCACTCTCGGCTACCGCACTCCCGAAAACATCG
AGCGCGCATACCACTCAACCCAGAACGAGGTGATAGAAGCAGCATAAAGCTGCTATAGTCCGTCTACGTCCGTGTCCGAGAAAAGGGCAGCACCTCA
AGTACAAAGACGAGGCGGTGAAGCTCGTCATCGATTCGGGCCGCCCCACATCGGCGGTCGCGAAAGATCTCGGTATCAACGAGGGAACGTTGGGGAGCTG
GGTCGCGACCTGGCGGCGGGCACACCAAAACGAAGAAGAACCGCTGTCGATGTCCGAACGGGCCCGGCTCCGCGAGCTGGAGAAGGAGAACCGGGAACTC
CGGATGGAGCGCGAGTTCCTCTCAAAAGCGACCGCCTTCTTCGCGCAACGCCATCAGTGACCGACAAGTACGAACTGATCGCCGCGGAGAAGGACAACTA
CCCGGTCACCAAGATGTGCCAGTGGCTCGTCGTGTCGACATCCGGTTTCTACGACTGGCACCGCAGGCCCGCGTCGACCCGGACCCGTCGGCACACCACC
CTCGACACCCACGTCCGGGCGGTGTTCGCCGCCTCCCGCCAGACCTACGGCGCCCGGCGGATCGCCGCCGCCCTGACCGCCTCCGGCCTGCGCGTCTCCG
TCCGCCTCGCCCGACGGATCATGCGCCGCGCAGGGCTGGAAGCCTGCCAGCCCAGGGCCTACCGCCGCACCACCATCCCCGGCCAGGCCCCGGCGCCCGC
CGACCACGTCCGCCGCGACTTCACCGCCACCCGCCCCGGCGAGAAGCTCATCGGGGACATCACCTATATCCGCACCGGCGAAGGATGGCTCTACCTCGCC
ACCGTCATCGACTGCTACTCCCGTAAAGTCGCCGGCTGGTCGATGGCCACCCACCTGCGCACCGAGCTCATCATCGACGCGTTCACCATGGCCGCCTCCC
GCACAACCCTCGCACCGAACGCGCTTTTCCACTCCGATCGTGGCGCCCAATACACGTCGGATGCCTACCATCGCGTCCTGAAACGCCATGGAGTACGCCC
GTCGGTCGGCGCCACCGGAGTCTGCTGGGACAATTCCGCTGCAGAGTCGTTCTTCGGAACGCTCAAGAACGAACTCGTGTACCGGGAAAACTACCCAACC
CGCCGCGCCGCACGTACCGCGATCGCCGAATACATCGAGGTCTTCTACAACCGGCAGCGGCTCCACTCCACTCTCGGCTACCGCACTCCCGAAAACATCG
AGCGCGCATACCACTCAACCCAGAACGAGGTGATAGAAGCAGCATAAAGCTGCTATAGTCCGTCTACGTCCGTGTCCGAGAAAAGGGCAGCACCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 70 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MSQSRRRYTPEYKDEAVKLVIDSGRPTSAVAKDLGINEGTLGSWVATWRRAHQNEEEPLSMSERARLRELEKENRELRMEREFLSKATAFFAQRHQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1191 bp | 396 aa | 57 | 1247 | + | No |
Description : Second part of the transposase
ORF sequence :
TEEINESVTQAIHTRVQRRGGEARHRFGPPHIGGRERSRYQRGNVGELGRDLAAGTPKRRRTAVDVRTGPAPRAGEGEPGTPDGARVPLKSDRLLRATPS
VMTDKYELIAAEKDNYPVTKMCQWLVVSTSGFYDWHRRPASTRTRRHTTLDTHVRAVFAASRQTYGARRIAAALTASGLRVSVRLARRIMRRAGLEACQP
RAYRRTTIPGQAPAPADHVRRDFTATRPGEKLIGDITYIRTGEGWLYLATVIDCYSRKVAGWSMATHLRTELIIDAFTMAASRTTLAPNALFHSDRGAQY
TSDAYHRVLKRHGVRPSVGATGVCWDNSAAESFFGTLKNELVYRENYPTRRAARTAIAEYIEVFYNRQRLHSTLGYRTPENIERAYHSTQNEVIEAA
VMTDKYELIAAEKDNYPVTKMCQWLVVSTSGFYDWHRRPASTRTRRHTTLDTHVRAVFAASRQTYGARRIAAALTASGLRVSVRLARRIMRRAGLEACQP
RAYRRTTIPGQAPAPADHVRRDFTATRPGEKLIGDITYIRTGEGWLYLATVIDCYSRKVAGWSMATHLRTELIIDAFTMAASRTTLAPNALFHSDRGAQY
TSDAYHRVLKRHGVRPSVGATGVCWDNSAAESFFGTLKNELVYRENYPTRRAARTAIAEYIEVFYNRQRLHSTLGYRTPENIERAYHSTQNEVIEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1178 bp | 393 aa | 70 | 1247 | + | Yes |
Chemistry : DDE
ORF sequence :
MSQSRRRYTPEYKDEAVKLVIDSGRPTSAVAKDLGINEGTLGSWVATWRRAHQNEEEPLSMSERARLRELEKENRELRMEREFLSKSDRLLRATPSVMTD
KYELIAAEKDNYPVTKMCQWLVVSTSGFYDWHRRPASTRTRRHTTLDTHVRAVFAASRQTYGARRIAAALTASGLRVSVRLARRIMRRAGLEACQPRAYR
RTTIPGQAPAPADHVRRDFTATRPGEKLIGDITYIRTGEGWLYLATVIDCYSRKVAGWSMATHLRTELIIDAFTMAASRTTLAPNALFHSDRGAQYTSDA
YHRVLKRHGVRPSVGATGVCWDNSAAESFFGTLKNELVYRENYPTRRAARTAIAEYIEVFYNRQRLHSTLGYRTPENIERAYHSTQNEVIEAA
KYELIAAEKDNYPVTKMCQWLVVSTSGFYDWHRRPASTRTRRHTTLDTHVRAVFAASRQTYGARRIAAALTASGLRVSVRLARRIMRRAGLEACQPRAYR
RTTIPGQAPAPADHVRRDFTATRPGEKLIGDITYIRTGEGWLYLATVIDCYSRKVAGWSMATHLRTELIIDAFTMAASRTTLAPNALFHSDRGAQYTSDA
YHRVLKRHGVRPSVGATGVCWDNSAAESFFGTLKNELVYRENYPTRRAARTAIAEYIEVFYNRQRLHSTLGYRTPENIERAYHSTQNEVIEAA
Blast result :
Comments
Genome in progress.
ISFsp8 is 63% (ORFA) aa similar to ISSod1 and 59% (ORFB) aa similar to ISCce3.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISFsp8 is 63% (ORFA) aa similar to ISSod1 and 59% (ORFB) aa similar to ISCce3.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank.