ISRba3
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005027 | ND | Rhodopirellula baltica | Rhodopirellula baltica SH 1 |
DNA section
IS Length : 1667 bp
Ends
IR Length : 18
IRL : CAACGCCGTGAACGATTTTGAGGCCGTGATTTGTGGTGTTTTGCTGAACT
IRR : CAACGCCGTGAACGATTTAGGCGACGGTACAAAATCCCAGTTTTTGAAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CAACGCCGTGAACGATTTTGAGGCCGTGATTTGTGGTGTTTTGCTGAACTGCCCTTGAAGTGTAAAAGCCGGATCCCCCTACCAACCAACTGAAACACTT
CAGTCAGAAAGGGATCCGGCTGTGTCAAAAAAACTCGATTCTCACGAATCAAGCAAGGCCAAAGAACATGCTCGACAAAGGAAATTCAATCGAGCCAAAC
GCAAGGTCAAGTCCGAATCTCAGCGACAGCACTATCGCAAAGCCCAGACGCGCAACGCCAAAGAAATCCGCAGTGCGATCGATTGGGTGCTGGGTGATGA
GCAGGCCGTGCCGACGCAGGCCCACGGCAACACTGGCTGGAAGACAATGACTCTGATGATCCAAGCCTTGCTGTGGATCTTCTCTGACAAGGACAAGCTC
AAGGATGCTTTTGATTCAGGCACCCGGCAATGCAAAAAGGTCCACGGGAGGATCGCATTTTCAAGTTACTCTGGACTGATCAAGGCGTTGGTTCGATGGA
CTCCATGGTTGTCCGAGGTTTTGCTTACTCGGATTCAGAAGCAAATTGAGACAACCGCCGGCAAGCTTTGGAGGACGACTGGTTGGGTCGTCATGGCTGT
CGATGGTTCTCGAGACACAACGCCGCGAACACTGAGTAACGAAAAAGCGTTTTGCGCTCCCAATCATGGTCACGGGAAAACGGCTCGGTATCGAAAAAAG
AAGACCAAAGGAATGCGCCGTCAGGCCATCGAGAAGAATCCACCGGCACCGCCGGTACCCCAGATTTGGATCACAATGATTTGGCACGTTGCCACGCAAC
TGACTTGGTGCTGGAAACTTGGGCCTAGCAATGCGTCCGAACGAGCACACGTGCAAGAGATGCTAGAAAACGGTGAATTCCCGGAGAAAACGTTGTTTAC
CGGTGACGCGGGATTCGTTGGCTACGAATTTTGGAAGAGCATTATCGATGGGGGGCACCACTTTCTCGTGCGCGTTGGCGCGAACGTCAACTTGCTGCAT
AGCCTCGGCTACGATGTCGAGCCTGACGAAGACAACCTTGTTTACTGCTGGCCCAAGGACAAACGCCGTGAAGGCATGCGTCCCCTGAAGCTTCGCATGA
TTCAGATCCAACTCGGCCGCAAAAAGGCAGTTCTCTTAACGAGCGTCTTGGATGAGAAGAAGCTCACCGACAAGCAAGCACTTGTGATTTACAAGAGCCG
CTGGGGAATCGAACTGGAATTTCGCAACCTAAAGCAAACGTATGGACGACGGCAACTTCGCTGCCGCCAGAGCATCCGGGCGTTGGTGGAGTTGCATTGG
TCGATCCTCAGTATTCTGATCGTCAAGCTGTATGCCTTGAAAGTACACCTCGCGAAGAAACGTCGCCGGGGTGATCCGGTCGCGATGCCTGGGCGAATCA
GCTTCGCAGGGGTGGTGCGTTCTTTCCAACGCATTCTTGAGAATCTCAACGAGACCGATCTCGAGGAACCGAGCTTATCGGAGATGCTGGGGGATGCGCT
GATCGATAATTACGAACGCAAGTCGAGCAAGGAGGCGAGGTATAAACCGAACAAGAAAGACAAACCTCGTTGCGGTCTCCCCAACGTCACTCGCGCCACG
AAAGAAGATCGCGATCGACTTCAAAAACTGGGATTTTGTACCGTCGCCTAAATCGTTCACGGCGTTG
CAGTCAGAAAGGGATCCGGCTGTGTCAAAAAAACTCGATTCTCACGAATCAAGCAAGGCCAAAGAACATGCTCGACAAAGGAAATTCAATCGAGCCAAAC
GCAAGGTCAAGTCCGAATCTCAGCGACAGCACTATCGCAAAGCCCAGACGCGCAACGCCAAAGAAATCCGCAGTGCGATCGATTGGGTGCTGGGTGATGA
GCAGGCCGTGCCGACGCAGGCCCACGGCAACACTGGCTGGAAGACAATGACTCTGATGATCCAAGCCTTGCTGTGGATCTTCTCTGACAAGGACAAGCTC
AAGGATGCTTTTGATTCAGGCACCCGGCAATGCAAAAAGGTCCACGGGAGGATCGCATTTTCAAGTTACTCTGGACTGATCAAGGCGTTGGTTCGATGGA
CTCCATGGTTGTCCGAGGTTTTGCTTACTCGGATTCAGAAGCAAATTGAGACAACCGCCGGCAAGCTTTGGAGGACGACTGGTTGGGTCGTCATGGCTGT
CGATGGTTCTCGAGACACAACGCCGCGAACACTGAGTAACGAAAAAGCGTTTTGCGCTCCCAATCATGGTCACGGGAAAACGGCTCGGTATCGAAAAAAG
AAGACCAAAGGAATGCGCCGTCAGGCCATCGAGAAGAATCCACCGGCACCGCCGGTACCCCAGATTTGGATCACAATGATTTGGCACGTTGCCACGCAAC
TGACTTGGTGCTGGAAACTTGGGCCTAGCAATGCGTCCGAACGAGCACACGTGCAAGAGATGCTAGAAAACGGTGAATTCCCGGAGAAAACGTTGTTTAC
CGGTGACGCGGGATTCGTTGGCTACGAATTTTGGAAGAGCATTATCGATGGGGGGCACCACTTTCTCGTGCGCGTTGGCGCGAACGTCAACTTGCTGCAT
AGCCTCGGCTACGATGTCGAGCCTGACGAAGACAACCTTGTTTACTGCTGGCCCAAGGACAAACGCCGTGAAGGCATGCGTCCCCTGAAGCTTCGCATGA
TTCAGATCCAACTCGGCCGCAAAAAGGCAGTTCTCTTAACGAGCGTCTTGGATGAGAAGAAGCTCACCGACAAGCAAGCACTTGTGATTTACAAGAGCCG
CTGGGGAATCGAACTGGAATTTCGCAACCTAAAGCAAACGTATGGACGACGGCAACTTCGCTGCCGCCAGAGCATCCGGGCGTTGGTGGAGTTGCATTGG
TCGATCCTCAGTATTCTGATCGTCAAGCTGTATGCCTTGAAAGTACACCTCGCGAAGAAACGTCGCCGGGGTGATCCGGTCGCGATGCCTGGGCGAATCA
GCTTCGCAGGGGTGGTGCGTTCTTTCCAACGCATTCTTGAGAATCTCAACGAGACCGATCTCGAGGAACCGAGCTTATCGGAGATGCTGGGGGATGCGCT
GATCGATAATTACGAACGCAAGTCGAGCAAGGAGGCGAGGTATAAACCGAACAAGAAAGACAAACCTCGTTGCGGTCTCCCCAACGTCACTCGCGCCACG
AAAGAAGATCGCGATCGACTTCAAAAACTGGGATTTTGTACCGTCGCCTAAATCGTTCACGGCGTTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1527 bp | 509 aa | 122 | 1648 | + | No |
Chemistry : DDE
ORF sequence :
MSKKLDSHESSKAKEHARQRKFNRAKRKVKSESQRQHYRKAQTRNAKEIRSAIDWVLGDEQAVPTQAHGNTGWKTMTLMIQALLWIFSDKDKLKDAFDSG
TRQCKKVHGRIAFSSYSGLIKALVRWTPWLSEVLLTRIQKQIETTAGKLWRTTGWVVMAVDGSRDTTPRTLSNEKAFCAPNHGHGKTARYRKKKTKGMRR
QAIEKNPPAPPVPQIWITMIWHVATQLTWCWKLGPSNASERAHVQEMLENGEFPEKTLFTGDAGFVGYEFWKSIIDGGHHFLVRVGANVNLLHSLGYDVE
PDEDNLVYCWPKDKRREGMRPLKLRMIQIQLGRKKAVLLTSVLDEKKLTDKQALVIYKSRWGIELEFRNLKQTYGRRQLRCRQSIRALVELHWSILSILI
VKLYALKVHLAKKRRRGDPVAMPGRISFAGVVRSFQRILENLNETDLEEPSLSEMLGDALIDNYERKSSKEARYKPNKKDKPRCGLPNVTRATKEDRDRL
QKLGFCTVA
TRQCKKVHGRIAFSSYSGLIKALVRWTPWLSEVLLTRIQKQIETTAGKLWRTTGWVVMAVDGSRDTTPRTLSNEKAFCAPNHGHGKTARYRKKKTKGMRR
QAIEKNPPAPPVPQIWITMIWHVATQLTWCWKLGPSNASERAHVQEMLENGEFPEKTLFTGDAGFVGYEFWKSIIDGGHHFLVRVGANVNLLHSLGYDVE
PDEDNLVYCWPKDKRREGMRPLKLRMIQIQLGRKKAVLLTSVLDEKKLTDKQALVIYKSRWGIELEFRNLKQTYGRRQLRCRQSIRALVELHWSILSILI
VKLYALKVHLAKKRRRGDPVAMPGRISFAGVVRSFQRILENLNETDLEEPSLSEMLGDALIDNYERKSSKEARYKPNKKDKPRCGLPNVTRATKEDRDRL
QKLGFCTVA
Blast result :
Comments
ISRba3 is 34% aa similar to ISBlma2. ISRba3 has been found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-101-D(N3)-102-E(C1).The copy number of ISRba3 in Rhodopirellula baltica SH 1 is 1.
References
1] Gloeckner,F.O., Kube,M., Bauer,M., Teeling,H., Lombardot,T., Ludwig,W., Gade,D., Beck,A., Borzym,K., Heitmann,K., Rabus,R., Schlesner,H., Amann,R. and Reinhardt,R. (2003) Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18