ISDpr3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ937768 | ND | delta proteobacterium | delta proteobacterium |
DNA section
IS Length : 1224 bp
Ends
IR Length : 18/37
IRL : TGGACACACCGAAGAGTTTCAGTTAAGAGAAAACAAGGTGGAGGTGTATC
IRR : TGGACACCCCACTAAGTTACACACGCTCTCCGTTCAAACTCGGCGGGCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGATTTAT | CTTTTTCGGG | 0 |
DNA sequence
TGGACACACCGAAGAGTTTCAGTTAAGAGAAAACAAGGTGGAGGTGTATCATGGTTATGGGGAAGGATAAGCGGTATTTTGATCGGGAGTTCAAGTATGA
AGCCTTACGGTTGGTAAACGAGGGGAAGCGATCTCTAGGAGATATTGCCAGGGATTTGGATATTCATCCAAACTTGCTGCATCAGTGGCGTCGTAAATAT
CGGGCAGATATGGAACATGCTTTTCCCGGTAAAGGTCATTTGAAGCCACCAGAGGAAGAGATTCGCCGTTTACAGCGAGAAAATGAGAATTTAAAGGAGG
AGAAGGAGATCTTAAAAAAAGCCTTGGCCATCTTCTCCAAACACCCAAAGTAAAGTATCAGTTCATAGAGAATCACCGTTCCGAATTTGCGGTGAAGAAG
ATGTGCCAGGTGCTGGGGGTTTCCAGGAGCGGTTATTATTTATGGGGAAAGCATAATAGGAGTGCCCGTCAGAAGCAGAATGAACGTTTGATGGTGCATA
TTAGGGAGGCTTACGCAAGAGGCCGCGGGGTTTATGGCAGCCCTCGGATCACAGCGGAACTTAAGGATAATGGGATTCCGTGCGGAAAGAACCGGATAGC
TCGTCTGATGAAGAGCAATGGGATCAAGGCTAAGACGAAACGGAGATTCAAGGCGACGAAGAGATTCAAACATGACTTTTTGGTGGCGGATAATCTTCTG
AATCAAAGATTTTCGGCAGACGTGGCCAATCAGATTTGGGTTTCTGACATTACCTTTATCTGGACAAGAGAAGGGTGGTTGTATCTAGCGGCGATTCTGG
ATATTTTTAACCGTAAGATTGTTGGATGGTCTATGGATAACAAACTGAGCCATGAAGTGATTGCCGATGCCCTGCACAAAGCGATCAGGCAGCGGAGACC
CAAGCCTGGTGTTTTGTTCCATTCAGATCGTGGAACGCAATATACCAGTTATGCTTTTCGGGATCTTATGGAGCAATACGGTTTTGTTCAGAGCATGAGT
TCTTCAGGAAATTGTTACGACAATGCCGTGATGGAGTCGTTTTTCCATACCTTAAAGACGGAACTTGTTTATTTTGAGAAATATCGGACCAGGCAGGAGG
CTCGTGGGGGCATATTTGAATATATTGAAGTATTTTATAATTGTGTTAGAAGACACTCGGCATTGAATTATTGCTCGCCCGCCGAGTTTGAACGGAGAGC
GTGTGTAACTTAGTGGGGTGTCCA
AGCCTTACGGTTGGTAAACGAGGGGAAGCGATCTCTAGGAGATATTGCCAGGGATTTGGATATTCATCCAAACTTGCTGCATCAGTGGCGTCGTAAATAT
CGGGCAGATATGGAACATGCTTTTCCCGGTAAAGGTCATTTGAAGCCACCAGAGGAAGAGATTCGCCGTTTACAGCGAGAAAATGAGAATTTAAAGGAGG
AGAAGGAGATCTTAAAAAAAGCCTTGGCCATCTTCTCCAAACACCCAAAGTAAAGTATCAGTTCATAGAGAATCACCGTTCCGAATTTGCGGTGAAGAAG
ATGTGCCAGGTGCTGGGGGTTTCCAGGAGCGGTTATTATTTATGGGGAAAGCATAATAGGAGTGCCCGTCAGAAGCAGAATGAACGTTTGATGGTGCATA
TTAGGGAGGCTTACGCAAGAGGCCGCGGGGTTTATGGCAGCCCTCGGATCACAGCGGAACTTAAGGATAATGGGATTCCGTGCGGAAAGAACCGGATAGC
TCGTCTGATGAAGAGCAATGGGATCAAGGCTAAGACGAAACGGAGATTCAAGGCGACGAAGAGATTCAAACATGACTTTTTGGTGGCGGATAATCTTCTG
AATCAAAGATTTTCGGCAGACGTGGCCAATCAGATTTGGGTTTCTGACATTACCTTTATCTGGACAAGAGAAGGGTGGTTGTATCTAGCGGCGATTCTGG
ATATTTTTAACCGTAAGATTGTTGGATGGTCTATGGATAACAAACTGAGCCATGAAGTGATTGCCGATGCCCTGCACAAAGCGATCAGGCAGCGGAGACC
CAAGCCTGGTGTTTTGTTCCATTCAGATCGTGGAACGCAATATACCAGTTATGCTTTTCGGGATCTTATGGAGCAATACGGTTTTGTTCAGAGCATGAGT
TCTTCAGGAAATTGTTACGACAATGCCGTGATGGAGTCGTTTTTCCATACCTTAAAGACGGAACTTGTTTATTTTGAGAAATATCGGACCAGGCAGGAGG
CTCGTGGGGGCATATTTGAATATATTGAAGTATTTTATAATTGTGTTAGAAGACACTCGGCATTGAATTATTGCTCGCCCGCCGAGTTTGAACGGAGAGC
GTGTGTAACTTAGTGGGGTGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 51 | 353 | + | No |
Description : First part of the transposase
ORF sequence :
MVMGKDKRYFDREFKYEALRLVNEGKRSLGDIARDLDIHPNLLHQWRRKYRADMEHAFPGKGHLKPPEEEIRRLQRENENLKEEKEILKKALAIFSKHPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 287 | 1213 | + | No |
Description : Second part of the transposase
ORF sequence :
EFKGGEGDLKKSLGHLLQTPKVKYQFIENHRSEFAVKKMCQVLGVSRSGYYLWGKHNRSARQKQNERLMVHIREAYARGRGVYGSPRITAELKDNGIPCG
KNRIARLMKSNGIKAKTKRRFKATKRFKHDFLVADNLLNQRFSADVANQIWVSDITFIWTREGWLYLAAILDIFNRKIVGWSMDNKLSHEVIADALHKAI
RQRRPKPGVLFHSDRGTQYTSYAFRDLMEQYGFVQSMSSSGNCYDNAVMESFFHTLKTELVYFEKYRTRQEARGGIFEYIEVFYNCVRRHSALNYCSPAE
FERRACVT
KNRIARLMKSNGIKAKTKRRFKATKRFKHDFLVADNLLNQRFSADVANQIWVSDITFIWTREGWLYLAAILDIFNRKIVGWSMDNKLSHEVIADALHKAI
RQRRPKPGVLFHSDRGTQYTSYAFRDLMEQYGFVQSMSSSGNCYDNAVMESFFHTLKTELVYFEKYRTRQEARGGIFEYIEVFYNCVRRHSALNYCSPAE
FERRACVT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 388 aa | 51 | 1213 | + | Yes |
Chemistry : DDE
ORF sequence :
MMVMGKDKRYFDREFKYEALRLVNEGKRSLGDIARDLDIHPNLLHQWRRKYRADMEHAFPGKGHLKPPEEEIRRLQRENENLKEEKEILKKSLGHLLQTP
KVKYQFIENHRSEFAVKKMCQVLGVSRSGYYLWGKHNRSARQKQNERLMVHIREAYARGRGVYGSPRITAELKDNGIPCGKNRIARLMKSNGIKAKTKRR
FKATKRFKHDFLVADNLLNQRFSADVANQIWVSDITFIWTREGWLYLAAILDIFNRKIVGWSMDNKLSHEVIADALHKAIRQRRPKPGVLFHSDRGTQYT
SYAFRDLMEQYGFVQSMSSSGNCYDNAVMESFFHTLKTELVYFEKYRTRQEARGGIFEYIEVFYNCVRRHSALNYCSPAEFERRACVT
KVKYQFIENHRSEFAVKKMCQVLGVSRSGYYLWGKHNRSARQKQNERLMVHIREAYARGRGVYGSPRITAELKDNGIPCGKNRIARLMKSNGIKAKTKRR
FKATKRFKHDFLVADNLLNQRFSADVANQIWVSDITFIWTREGWLYLAAILDIFNRKIVGWSMDNKLSHEVIADALHKAIRQRRPKPGVLFHSDRGTQYT
SYAFRDLMEQYGFVQSMSSSGNCYDNAVMESFFHTLKTELVYFEKYRTRQEARGGIFEYIEVFYNCVRRHSALNYCSPAEFERRACVT
Blast result :
Comments
ISDpr3 is 64 % (ORFA) aa similar to ISApr1 and 66% (ORFB) aa similar to ISAzvi9. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Nesbo,C.L., Boucher,Y., Dlutek,M. and Doolittle,W.F. (2005) Environ. Microbiol. 7 (12), 2011-2026.