ISCte2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAUJ01000001 | ND | Comamonas testosteroni | Comamonas testosteroni KF |
DNA section
IS Length : 1301 bp
Ends
IR Length : 25/39
IRL : TGAGGTTGCCCCCGAAAAGTGGAGCTCTTAGCCTTGTTGAAAATATCGAC
IRR : TGACCTTGCCCCTGTTCCGTGTAGTTCTTAGCCCAATGGTCACGCGGCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTGAACAAG | ATT | TGGACGCCTC | 3 |
DNA sequence
TGAGGTTGCCCCCGAAAAGTGGAGCTCTTAGCCTTGTTGAAAATATCGACAAGGAGCCGAGAGATGACACACAAGCAGTTGCGTGCGCAGTACACGCAAG
AGTTCAAAATGGAGGCGGTGCGCCAGGTGCGGGCGGGTCAGGCCATTGCCGTGGTGGCCAAGGTCTTGGGCATACCCAAAGCCAGCCTGGGCAATTGGGT
TCGCCAGTCGGCCAAGGGCGATTTGAGCGGGGCGGGTGCTGATGACAAGGTTGCCAGGGTGTCCCCAGAGCAGATGGAGCTGGCCCGCCTGAGGGCAGAG
AACGCGCGCTTGAGGATGGAGCGCGACATCGCAAAAAAAGCCGCGGCGTACTTCGCGCAGGACACGCTGCGGGGTACGCCTGGATTCACCAAATGAGAGG
GCTGTATCCGGTCAGCCTGGCCTGCGAGGTGCTGGAAGTCAGCGCCAGCGGGTATTTCAACTGGCTGCGCAGGCGCCAGGCCAGCCGCAGTGGGCCGTCC
GGGCGCCACAGCGACGAGGCCCTGTTGGCTCACATCCGTGCCATCCACGCCGAAGTCAAGGGCGAATATGGCTGGCCACGCATGCACAAGGAGTTGCTGG
CGCGGGGCATTCGGGTGGGCAAAGACAGGGTGCGCAAGCTGATGAAACAGCACGGCATCAAGGCCAAGACCAAACGCAAGTTCGTGGTGACCACCGACAG
CCGCCATAACCTACCCGTGGCGCCCGACCTGGTGCAGCGGCGCTTCAACCCCCAGGGGCCCAACCAGCTCTGGAGTGGCGACATCACCTACATCGCCACG
GACGAGGGCTGGCTGTACCTGGCCGCTGTCATCGACCTGTTTGGCCGCCAGGTGGTGGGCTGGAGCCTTCAGCCCCACATGCAAACCAGTCTGGTCAAGG
ACGCATTGGCCATGGCGTGGTGGCGTCGGCGGCCTGCGGCTGGCTTGATATTCCACAGCGACAGGGGCAGTCAATATTGCAGCGGGGAGTTCCAGGCCAT
TTTGAGAGCCTGGGGCATGCGCTCATCGATGAGCAGAAAGGGGAACTGCTGGGACAACGCGCCAACGGAGAGCTTCTGGGGTCGGCTGAAAACGGCCAGT
GTGCATGGTCAAAAATTCGCCACCCGAGAGCAGGCCAGGCAGGCTGTGATGGACTGGATGGCTTTCTACAATCACCGCAGGCTGCATTCGTCGCTGGGCT
ATCTCAGCCCGATGCAGTTCGAGCAACGCTGGTACGAGGCACAGCGTAAAAAAGCCGCGTGACCATTGGGCTAAGAACTACACGGAACAGGGGCAAGGTC
A
AGTTCAAAATGGAGGCGGTGCGCCAGGTGCGGGCGGGTCAGGCCATTGCCGTGGTGGCCAAGGTCTTGGGCATACCCAAAGCCAGCCTGGGCAATTGGGT
TCGCCAGTCGGCCAAGGGCGATTTGAGCGGGGCGGGTGCTGATGACAAGGTTGCCAGGGTGTCCCCAGAGCAGATGGAGCTGGCCCGCCTGAGGGCAGAG
AACGCGCGCTTGAGGATGGAGCGCGACATCGCAAAAAAAGCCGCGGCGTACTTCGCGCAGGACACGCTGCGGGGTACGCCTGGATTCACCAAATGAGAGG
GCTGTATCCGGTCAGCCTGGCCTGCGAGGTGCTGGAAGTCAGCGCCAGCGGGTATTTCAACTGGCTGCGCAGGCGCCAGGCCAGCCGCAGTGGGCCGTCC
GGGCGCCACAGCGACGAGGCCCTGTTGGCTCACATCCGTGCCATCCACGCCGAAGTCAAGGGCGAATATGGCTGGCCACGCATGCACAAGGAGTTGCTGG
CGCGGGGCATTCGGGTGGGCAAAGACAGGGTGCGCAAGCTGATGAAACAGCACGGCATCAAGGCCAAGACCAAACGCAAGTTCGTGGTGACCACCGACAG
CCGCCATAACCTACCCGTGGCGCCCGACCTGGTGCAGCGGCGCTTCAACCCCCAGGGGCCCAACCAGCTCTGGAGTGGCGACATCACCTACATCGCCACG
GACGAGGGCTGGCTGTACCTGGCCGCTGTCATCGACCTGTTTGGCCGCCAGGTGGTGGGCTGGAGCCTTCAGCCCCACATGCAAACCAGTCTGGTCAAGG
ACGCATTGGCCATGGCGTGGTGGCGTCGGCGGCCTGCGGCTGGCTTGATATTCCACAGCGACAGGGGCAGTCAATATTGCAGCGGGGAGTTCCAGGCCAT
TTTGAGAGCCTGGGGCATGCGCTCATCGATGAGCAGAAAGGGGAACTGCTGGGACAACGCGCCAACGGAGAGCTTCTGGGGTCGGCTGAAAACGGCCAGT
GTGCATGGTCAAAAATTCGCCACCCGAGAGCAGGCCAGGCAGGCTGTGATGGACTGGATGGCTTTCTACAATCACCGCAGGCTGCATTCGTCGCTGGGCT
ATCTCAGCCCGATGCAGTTCGAGCAACGCTGGTACGAGGCACAGCGTAAAAAAGCCGCGTGACCATTGGGCTAAGAACTACACGGAACAGGGGCAAGGTC
A
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 330 bp | 110 aa | 63 | 392 | + | No |
Description : First part of the transposase
ORF sequence :
MTHKQLRAQYTQEFKMEAVRQVRAGQAIAVVAKVLGIPKASLGNWVRQSAKGDLSGAGADDKVARVSPEQMELARLRAENARLRMERDIAKKAAAYFAQD
TLRGTPGFTK
TLRGTPGFTK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 246 | 1262 | + | No |
Description : Second part of the transposase
ORF sequence :
QGCQGVPRADGAGPPEGRERALEDGARHRKKSRGVLRAGHAAGYAWIHQMRGLYPVSLACEVLEVSASGYFNWLRRRQASRSGPSGRHSDEALLAHIRAI
HAEVKGEYGWPRMHKELLARGIRVGKDRVRKLMKQHGIKAKTKRKFVVTTDSRHNLPVAPDLVQRRFNPQGPNQLWSGDITYIATDEGWLYLAAVIDLFG
RQVVGWSLQPHMQTSLVKDALAMAWWRRRPAAGLIFHSDRGSQYCSGEFQAILRAWGMRSSMSRKGNCWDNAPTESFWGRLKTASVHGQKFATREQARQA
VMDWMAFYNHRRLHSSLGYLSPMQFEQRWYEAQRKKAA
HAEVKGEYGWPRMHKELLARGIRVGKDRVRKLMKQHGIKAKTKRKFVVTTDSRHNLPVAPDLVQRRFNPQGPNQLWSGDITYIATDEGWLYLAAVIDLFG
RQVVGWSLQPHMQTSLVKDALAMAWWRRRPAAGLIFHSDRGSQYCSGEFQAILRAWGMRSSMSRKGNCWDNAPTESFWGRLKTASVHGQKFATREQARQA
VMDWMAFYNHRRLHSSLGYLSPMQFEQRWYEAQRKKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1200 bp | 399 aa | 63 | 1262 | + | Yes |
Chemistry : DDE
ORF sequence :
MTHKQLRAQYTQEFKMEAVRQVRAGQAIAVVAKVLGIPKASLGNWVRQSAKGDLSGAGADDKVARVSPEQMELARLRAENARLRMERDIAKKSRGVLRAG
HAAGYAWIHQMRGLYPVSLACEVLEVSASGYFNWLRRRQASRSGPSGRHSDEALLAHIRAIHAEVKGEYGWPRMHKELLARGIRVGKDRVRKLMKQHGIK
AKTKRKFVVTTDSRHNLPVAPDLVQRRFNPQGPNQLWSGDITYIATDEGWLYLAAVIDLFGRQVVGWSLQPHMQTSLVKDALAMAWWRRRPAAGLIFHSD
RGSQYCSGEFQAILRAWGMRSSMSRKGNCWDNAPTESFWGRLKTASVHGQKFATREQARQAVMDWMAFYNHRRLHSSLGYLSPMQFEQRWYEAQRKKAA
HAAGYAWIHQMRGLYPVSLACEVLEVSASGYFNWLRRRQASRSGPSGRHSDEALLAHIRAIHAEVKGEYGWPRMHKELLARGIRVGKDRVRKLMKQHGIK
AKTKRKFVVTTDSRHNLPVAPDLVQRRFNPQGPNQLWSGDITYIATDEGWLYLAAVIDLFGRQVVGWSLQPHMQTSLVKDALAMAWWRRRPAAGLIFHSD
RGSQYCSGEFQAILRAWGMRSSMSRKGNCWDNAPTESFWGRLKTASVHGQKFATREQARQAVMDWMAFYNHRRLHSSLGYLSPMQFEQRWYEAQRKKAA
Blast result :
Comments
ISCte2 is 76% (ORFA) and 69% (ORFB) aa similar to ISBcen17.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
Genome in progress.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
Genome in progress.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.