ISCps2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003910 | ND | Colwellia psychrerythraea | Colwellia psychrerythraea 34H |
DNA section
IS Length : 1248 bp
Ends
IR Length : 21/33
IRL : TAGTCTTACCCCATTTTCGTGGACACACTATAATTGTTGTAGAGATACAA
IRR : TGATCTTGCCCCATAAAAATGGACACTAATTAAGAATATTTCCTTCAGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAGTCTTACCCCATTTTCGTGGACACACTATAATTGTTGTAGAGATACAACAGGAGGTGTTTCATGACAAAATCCAAAAGTTATCAAAGATACCCTGCTG
AGTTTAAACGTATGGCGTTACTCAAAGCTTCTGAAGACGGTGTTACTGCTAATAGTGTTTGCGAAGAACTCGGGATCAGTTTTAGGCAACTAAGTCGTTG
GCGTGATGAGTTTCGCTTGAAAGGTGATGATGCTTTCAAAGGGAATAAACAAACTAGCAATAATGAATTAACTAAGTTGAAGCAAGAGCTGGCTAAAGTA
AAAAAAGAGCGTGATTTTTTAAAAGATGCGGTGGTGTTCTTCGCCAAGGAGTCCGGTTAAAGTTTTGTTGTATTAAGCGGCACCGCAACAGTTATCCCGT
AAAAATGATGTGTGCTCTATTGGATATTTCACGTAGTGGTTATTACGCCTGGCTAAGTCGGCCACTATGTAAAACCAAGCAAGAGAATATTCAGTTAACA
GAGCAGATATCAAAAGTATTTGAGCAAAGCAGATGTGTATATGGAGCTCCAAGAATCAGGGCTGCGCTTAACGCTGACGGGCAAGCTTGTGGTAAAAACA
GAGTGGCTAGGTTAATGCGTGTTTTGCAATTAAAAGGCCGTCCTAAGCGTGTATTTAGACGTTCAGCTAAGTCTAATCCATACGTTGAGCCTGCGCCAAA
TTTGCTACACCAAAACTTTGTTTCAAAGGCGGTAAATCAAGTTTGGTCATCAGACATAACTTATATACAAACGAAACAAGGTTTTGTTTATTTAGCCGTC
GTAATGGACTTGTACTCACGTAAAATTATTGGGTGGTCAATGGATAAAAACATGGGCCGTCACATTGCAATGAACGCGTTAGGCATGGCTGTGGCAGCAA
GAAATCCGAGTGATGGCCTCATCATTCATTCTGATAGAGGATCTCAATACATCAGCGATGACTATCAGCAGATGTTAAACGAAAATGGCATCCTATGCAG
CATGAGCGCTAGAGGAAACTGCTATGACAATGCTGTTGTAGAAAGTTTTTTCGGGTCACTTAAAAGAGAACGGATTCGTCGGTACAACTACAGAACAAGA
CAAGAAGCGCAAATAGATGTGTTTGATTATATTGAATGCTTCTATAATAAAAGGAGGCTTCACAGCTATCTAAAGTATAAAAACCCTTCGGACTTTGAAG
CTGAAGGAAATATTCTTAATTAGTGTCCATTTTTATGGGGCAAGATCA
AGTTTAAACGTATGGCGTTACTCAAAGCTTCTGAAGACGGTGTTACTGCTAATAGTGTTTGCGAAGAACTCGGGATCAGTTTTAGGCAACTAAGTCGTTG
GCGTGATGAGTTTCGCTTGAAAGGTGATGATGCTTTCAAAGGGAATAAACAAACTAGCAATAATGAATTAACTAAGTTGAAGCAAGAGCTGGCTAAAGTA
AAAAAAGAGCGTGATTTTTTAAAAGATGCGGTGGTGTTCTTCGCCAAGGAGTCCGGTTAAAGTTTTGTTGTATTAAGCGGCACCGCAACAGTTATCCCGT
AAAAATGATGTGTGCTCTATTGGATATTTCACGTAGTGGTTATTACGCCTGGCTAAGTCGGCCACTATGTAAAACCAAGCAAGAGAATATTCAGTTAACA
GAGCAGATATCAAAAGTATTTGAGCAAAGCAGATGTGTATATGGAGCTCCAAGAATCAGGGCTGCGCTTAACGCTGACGGGCAAGCTTGTGGTAAAAACA
GAGTGGCTAGGTTAATGCGTGTTTTGCAATTAAAAGGCCGTCCTAAGCGTGTATTTAGACGTTCAGCTAAGTCTAATCCATACGTTGAGCCTGCGCCAAA
TTTGCTACACCAAAACTTTGTTTCAAAGGCGGTAAATCAAGTTTGGTCATCAGACATAACTTATATACAAACGAAACAAGGTTTTGTTTATTTAGCCGTC
GTAATGGACTTGTACTCACGTAAAATTATTGGGTGGTCAATGGATAAAAACATGGGCCGTCACATTGCAATGAACGCGTTAGGCATGGCTGTGGCAGCAA
GAAATCCGAGTGATGGCCTCATCATTCATTCTGATAGAGGATCTCAATACATCAGCGATGACTATCAGCAGATGTTAAACGAAAATGGCATCCTATGCAG
CATGAGCGCTAGAGGAAACTGCTATGACAATGCTGTTGTAGAAAGTTTTTTCGGGTCACTTAAAAGAGAACGGATTCGTCGGTACAACTACAGAACAAGA
CAAGAAGCGCAAATAGATGTGTTTGATTATATTGAATGCTTCTATAATAAAAGGAGGCTTCACAGCTATCTAAAGTATAAAAACCCTTCGGACTTTGAAG
CTGAAGGAAATATTCTTAATTAGTGTCCATTTTTATGGGGCAAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 64 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MTKSKSYQRYPAEFKRMALLKASEDGVTANSVCEELGISFRQLSRWRDEFRLKGDDAFKGNKQTSNNELTKLKQELAKVKKERDFLKDAVVFFAKESG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 819 bp | 272 aa | 405 | 1223 | + | No |
Description : Second part of the transposase
ORF sequence :
MMCALLDISRSGYYAWLSRPLCKTKQENIQLTEQISKVFEQSRCVYGAPRIRAALNADGQACGKNRVARLMRVLQLKGRPKRVFRRSAKSNPYVEPAPNL
LHQNFVSKAVNQVWSSDITYIQTKQGFVYLAVVMDLYSRKIIGWSMDKNMGRHIAMNALGMAVAARNPSDGLIIHSDRGSQYISDDYQQMLNENGILCSM
SARGNCYDNAVVESFFGSLKRERIRRYNYRTRQEAQIDVFDYIECFYNKRRLHSYLKYKNPSDFEAEGNILN
LHQNFVSKAVNQVWSSDITYIQTKQGFVYLAVVMDLYSRKIIGWSMDKNMGRHIAMNALGMAVAARNPSDGLIIHSDRGSQYISDDYQQMLNENGILCSM
SARGNCYDNAVVESFFGSLKRERIRRYNYRTRQEAQIDVFDYIECFYNKRRLHSYLKYKNPSDFEAEGNILN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 64 | 1223 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKSKSYQRYPAEFKRMALLKASEDGVTANSVCEELGISFRQLSRWRDEFRLKGDDAFKGNKQTSNNELTKLKQELAKVKKERDFLKRCGGVLRQGVRLK
FCCIKRHRNSYPVKMMCALLDISRSGYYAWLSRPLCKTKQENIQLTEQISKVFEQSRCVYGAPRIRAALNADGQACGKNRVARLMRVLQLKGRPKRVFRR
SAKSNPYVEPAPNLLHQNFVSKAVNQVWSSDITYIQTKQGFVYLAVVMDLYSRKIIGWSMDKNMGRHIAMNALGMAVAARNPSDGLIIHSDRGSQYISDD
YQQMLNENGILCSMSARGNCYDNAVVESFFGSLKRERIRRYNYRTRQEAQIDVFDYIECFYNKRRLHSYLKYKNPSDFEAEGNILN
FCCIKRHRNSYPVKMMCALLDISRSGYYAWLSRPLCKTKQENIQLTEQISKVFEQSRCVYGAPRIRAALNADGQACGKNRVARLMRVLQLKGRPKRVFRR
SAKSNPYVEPAPNLLHQNFVSKAVNQVWSSDITYIQTKQGFVYLAVVMDLYSRKIIGWSMDKNMGRHIAMNALGMAVAARNPSDGLIIHSDRGSQYISDD
YQQMLNENGILCSMSARGNCYDNAVVESFFGSLKRERIRRYNYRTRQEAQIDVFDYIECFYNKRRLHSYLKYKNPSDFEAEGNILN
Blast result :
Comments
There are 2 copies of ISCps2 in Colwellia psychrerythraea 34H.ISCps2 is 55%(ORFA) aa similar to ISRgn1 and 66% (ORFB) to ISEhe3. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
References
1] Methe,B.A., Nelson,K.E., Deming,J.W., Momen,B., Melamud,E., Zhang,X., Moult,J., Madupu,R., Nelson,W.C., Dodson,R.J., Brinkac,L.M., Daugherty,S.C., Durkin,A.S., Deboy,R.T., Kolonay,J.F., Sullivan,S.A., Zhou,L., Davidsen,T.M., Wu,M., Huston,A.L., Lewis,M., Weaver,B., Weidman,J.F., Khouri,H., Utterback,T.R., Feldblyum,T.V. and Fraser,C.M. (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (31), 10913-10918 .