ISBcen21

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAVA01000021 ND Burkholderia cenocepacia
Burkholderia cenocepacia MC0-3
DNA section
IS Length : 1231 bp

Ends


IR Length : 31/41

IRL : TGGACCGGCCAGCCTTTCGTAGACATCTTCGAGCCATAATTTATGGCAAC
IRR : TGGACCGGCCAGAGTTTCATAGACACTCACGAGCCGTTTAACGCGTAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
ACGAGTTGTCGCTCGCGAGC0

DNA sequence

TGGACCGGCCAGCCTTTCGTAGACATCTTCGAGCCATAATTTATGGCAACCGAGGAGGTCAGTATGAGCGGCAAGCGGTACACGGATGAGTTCAAGATTG
AGGCAGTCAAGCAAGTCACCGAGCGAGGTCATTCGGTAGCTGATGTGGCGCAGCGGCTGGGCATCACGACCCACAGCCTTTACGCCTGGAAACTGAAGTT
CGGCAGGTCCGACGTCGTGCGGCGATCGGAGCTGGATCAGAGCGCCGAGGTTCGGCGACTGAAGGCCGAGCTCAAGCGCATGACCGAGGAGCGCGACATT
CTAAAAAAAGCCGCCGCGTACTTTGCAAAGGGATAGCGGCAAGGTACGCGTTCATGCGAGCCCATCTCGGCGAGTTCCGCCTGCATTCAATGTGCCGAGT
CCTTGGCGTGCATCGCAGCGGCTATTACGTCTGGCAGCGGCAGACAGTCGGTGTCCGCAAGCGTGAGGATGATCGATTGCTCGGTCTGATCAAGCATTCC
TGGCTGGCCAGCGGCGGCGTCTATGGCTACCGCAAGGTCACGGTGGATTTGCGTGAATCGGGAGAAACGTGCAGCCGTCACCGCGTCCATCGCCTCATGA
AAGGCGAAGGTCTGCGGGCAGAAGTGGGCTATGGCAGCAAGCCCCGCTATCGGGGTGGTCCGGTCGGCGTAATCGCCAACGTGCTGAACCGTGAGTTCGC
GCCAACCGCACCGAACCGGGTCTGGGTAACGGACATCACGTACATCCGGACCTACGAGGGCTGGCTGTATCTGGCGGCTGTTATGGATCTGTACTCGCGC
CAGATCGTCGGTTGGGCTACGCGCTCGACGATGACCAGCGATCTGGTTTTACAGGCGCTGTTGGCTGCGGTTTGGAAACGCAAACCGGGACCCGGTGTCA
TGGTGCATTCCGACCAGGGCAGTCAATTCACGAGCGATGACTGGCAGTCATTCCTGAAGGCACACCATATGGTGCCAAGCATGAGTCGGCGCGGTAATTG
CCACGACAATGCCGTGGCCGAGAGCTTCTTCAGTGCCCTAAAGAAGGAGCGAATCAAGCGGCGCATCTATCCGAGTCGGGCGACCGCGACCTCGGACTTG
TTCGATTACATCGAAATGTTTTATAACCCGGTTCGTCGGCATGGTTCCGCCGGCGATCTTTCACCGATTGAGTTCGAGCGGCGCTACGCGTTAAACGGCT
CGTGAGTGTCTATGAAACTCTGGCCGGTCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
297 bp98 aa40336+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MATEEVSMSGKRYTDEFKIEAVKQVTERGHSVADVAQRLGITTHSLYAWKLKFGRSDVVRRSELDQSAEVRRLKAELKRMTEERDILKKAAAYFAKG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1062 bp353 aa1441205+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

CGAAAGHHDPQPLRLETEVRQVRRRAAIGAGSERRGSATEGRAQAHDRGARHSKKSRRVLCKGIAARYAFMRAHLGEFRLHSMCRVLGVHRSGYYVWQRQ
TVGVRKREDDRLLGLIKHSWLASGGVYGYRKVTVDLRESGETCSRHRVHRLMKGEGLRAEVGYGSKPRYRGGPVGVIANVLNREFAPTAPNRVWVTDITY
IRTYEGWLYLAAVMDLYSRQIVGWATRSTMTSDLVLQALLAAVWKRKPGPGVMVHSDQGSQFTSDDWQSFLKAHHMVPSMSRRGNCHDNAVAESFFSALK
KERIKRRIYPSRATATSDLFDYIEMFYNPVRRHGSAGDLSPIEFERRYALNGS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1166 bp387 aa401205+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATEEVSMSGKRYTDEFKIEAVKQVTERGHSVADVAQRLGITTHSLYAWKLKFGRSDVVRRSELDQSAEVRRLKAELKRMTEERDILKKSRRVLCKGIAA
RYAFMRAHLGEFRLHSMCRVLGVHRSGYYVWQRQTVGVRKREDDRLLGLIKHSWLASGGVYGYRKVTVDLRESGETCSRHRVHRLMKGEGLRAEVGYGSK
PRYRGGPVGVIANVLNREFAPTAPNRVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTMTSDLVLQALLAAVWKRKPGPGVMVHSDQGSQFTSDD
WQSFLKAHHMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYPSRATATSDLFDYIEMFYNPVRRHGSAGDLSPIEFERRYALNGS

 

Blast result :
Comments
ISBcen21 is 90% (ORFA) aa similar to ISXac3 and 76% (ORFB) aa similar to ISAisp2. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.