ISBph1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAUG01000003 | ND | Burkholderia phymatum | Burkholderia phymatum STM815 |
DNA section
IS Length : 1290 bp
Ends
IR Length : 29/38
IRL : TGAGCTTCCCCTGAAATTTCGCCTTCCAGCAGGTCGCGTATAAACTCGAC
IRR : TGAGCTTCCCTCGGTTTTTCGCCTTCCAGAGGCCCCGGGTTATGCAGCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCGTACTGTT | ATACCATCGA | 0 |
DNA sequence
TGAGCTTCCCCTGAAATTTCGCCTTCCAGCAGGTCGCGTATAAACTCGACCCAAAGGAAGGAAAGAAAAGATGTTCAAGGTACCGCACCAGGTGTACACG
GCAGAGTTCAAGGAAGCGGCCGTGCAACGAGTTAAGGACGGACAAAGCGTGAGCGCTGTGGCCCGTGAACTGGGCATGTCGATGCAGACACTGCGCAACT
GGCTCAAAGCGTCGGAGGCCGGCAAGCTTAATGGCCCCGGGGCAAAGGTCGTCACGGCCGAACAGATGGAACTCTCGCGTCTGCGAGCGGAGAACAAGCG
CTTGCAGATGGAGCTGGAAATCGCAAAAAAAGCGGCGGCGTTCTTCGCGAAGGACCTCCTGTGAAGTACGCCTGGATTGACACGCAGTGCCGGCAGTATC
CTCTGTCGGTGCTGTGCGAGGTCCTTGCTGTAAGCATCAACGGCTATCGCGCCTGGAAGCGCGGCGGCACGCCTGAGCGACAGCGGCTGACTGATGCCCA
GTTGCTCACGCTGATTCGAACGATTCATGCCAAGGTCAAAGGCGCTTACGGTTCGCCGCGCATGACCGACGAGGTGCGCTCTCGCGGCTTTCCGGCCAGC
AAAGCACGTGTGGAGCGGCTGATGAGCGCCAACGGTATCCGAGCCCGTCACAAGCGCCGTTATCGGGTGACAACCGATTCCCGGCACAAGCTGCCGGTCG
CACCGAACGTGTTGAATCGCGACTTCACGCCGGCCGAGCCCAACCAGGTGTTTACCTCGGATATCACGTACATCTGGACGGACGAAGGCTGGCTGTATCT
GGCGGTCACTCTCGATCTGTTCAATCGCGAGGTCGTGGGCTGGTCCGTCAAGCCACGCATGACGGCGGACCTCGTGACCGACGCGCTGACGATGGCATGG
TTCCGCCGCCGGCCCGCGCCTGGCACGCTGCATCATTCGGACAGAGGCAGTCAATACGCGAGCCACGACTTCCAGCGCAAGCTGACTTCGTACGGCATGC
GATGCTCGATGAGCCGCAAAGGCAATTGCTGGGATAACGCGCCGACGGAAAGCTTCTTCAACAGCCTGAAGAACGAGCGGGTACACGGCACGAGGTATCG
CACGCATCATGAAGCTGTAGCGGACTTGTTCGAATACATAGAAGTGTTTTATAACCGCAGTCGTCGTCATTCATCGCTCGGCTTCATGTCGCCAACTCAG
TTCATGCAAAACTGGCTTGCGGCTCAGCGGACAAAGGATACGGCTGCATAACCCGGGGCCTCTGGAAGGCGAAAAACCGAGGGAAGCTCA
GCAGAGTTCAAGGAAGCGGCCGTGCAACGAGTTAAGGACGGACAAAGCGTGAGCGCTGTGGCCCGTGAACTGGGCATGTCGATGCAGACACTGCGCAACT
GGCTCAAAGCGTCGGAGGCCGGCAAGCTTAATGGCCCCGGGGCAAAGGTCGTCACGGCCGAACAGATGGAACTCTCGCGTCTGCGAGCGGAGAACAAGCG
CTTGCAGATGGAGCTGGAAATCGCAAAAAAAGCGGCGGCGTTCTTCGCGAAGGACCTCCTGTGAAGTACGCCTGGATTGACACGCAGTGCCGGCAGTATC
CTCTGTCGGTGCTGTGCGAGGTCCTTGCTGTAAGCATCAACGGCTATCGCGCCTGGAAGCGCGGCGGCACGCCTGAGCGACAGCGGCTGACTGATGCCCA
GTTGCTCACGCTGATTCGAACGATTCATGCCAAGGTCAAAGGCGCTTACGGTTCGCCGCGCATGACCGACGAGGTGCGCTCTCGCGGCTTTCCGGCCAGC
AAAGCACGTGTGGAGCGGCTGATGAGCGCCAACGGTATCCGAGCCCGTCACAAGCGCCGTTATCGGGTGACAACCGATTCCCGGCACAAGCTGCCGGTCG
CACCGAACGTGTTGAATCGCGACTTCACGCCGGCCGAGCCCAACCAGGTGTTTACCTCGGATATCACGTACATCTGGACGGACGAAGGCTGGCTGTATCT
GGCGGTCACTCTCGATCTGTTCAATCGCGAGGTCGTGGGCTGGTCCGTCAAGCCACGCATGACGGCGGACCTCGTGACCGACGCGCTGACGATGGCATGG
TTCCGCCGCCGGCCCGCGCCTGGCACGCTGCATCATTCGGACAGAGGCAGTCAATACGCGAGCCACGACTTCCAGCGCAAGCTGACTTCGTACGGCATGC
GATGCTCGATGAGCCGCAAAGGCAATTGCTGGGATAACGCGCCGACGGAAAGCTTCTTCAACAGCCTGAAGAACGAGCGGGTACACGGCACGAGGTATCG
CACGCATCATGAAGCTGTAGCGGACTTGTTCGAATACATAGAAGTGTTTTATAACCGCAGTCGTCGTCATTCATCGCTCGGCTTCATGTCGCCAACTCAG
TTCATGCAAAACTGGCTTGCGGCTCAGCGGACAAAGGATACGGCTGCATAACCCGGGGCCTCTGGAAGGCGAAAAACCGAGGGAAGCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 71 | 364 | + | No |
Description : First part of the transposase
ORF sequence :
MFKVPHQVYTAEFKEAAVQRVKDGQSVSAVARELGMSMQTLRNWLKASEAGKLNGPGAKVVTAEQMELSRLRAENKRLQMELEIAKKAAAFFAKDLL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1020 bp | 339 aa | 232 | 1251 | + | No |
Description : Second part of the transposase
ORF sequence :
WPRGKGRHGRTDGTLASASGEQALADGAGNRKKSGGVLREGPPVKYAWIDTQCRQYPLSVLCEVLAVSINGYRAWKRGGTPERQRLTDAQLLTLIRTIHA
KVKGAYGSPRMTDEVRSRGFPASKARVERLMSANGIRARHKRRYRVTTDSRHKLPVAPNVLNRDFTPAEPNQVFTSDITYIWTDEGWLYLAVTLDLFNRE
VVGWSVKPRMTADLVTDALTMAWFRRRPAPGTLHHSDRGSQYASHDFQRKLTSYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHGTRYRTHHEAVADLF
EYIEVFYNRSRRHSSLGFMSPTQFMQNWLAAQRTKDTAA
KVKGAYGSPRMTDEVRSRGFPASKARVERLMSANGIRARHKRRYRVTTDSRHKLPVAPNVLNRDFTPAEPNQVFTSDITYIWTDEGWLYLAVTLDLFNRE
VVGWSVKPRMTADLVTDALTMAWFRRRPAPGTLHHSDRGSQYASHDFQRKLTSYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHGTRYRTHHEAVADLF
EYIEVFYNRSRRHSSLGFMSPTQFMQNWLAAQRTKDTAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1181 bp | 393 aa | 71 | 1251 | + | Yes |
Chemistry : DDE
ORF sequence :
MFKVPHQVYTAEFKEAAVQRVKDGQSVSAVARELGMSMQTLRNWLKASEAGKLNGPGAKVVTAEQMELSRLRAENKRLQMELEIAKKSGGVLREGPPVKY
AWIDTQCRQYPLSVLCEVLAVSINGYRAWKRGGTPERQRLTDAQLLTLIRTIHAKVKGAYGSPRMTDEVRSRGFPASKARVERLMSANGIRARHKRRYRV
TTDSRHKLPVAPNVLNRDFTPAEPNQVFTSDITYIWTDEGWLYLAVTLDLFNREVVGWSVKPRMTADLVTDALTMAWFRRRPAPGTLHHSDRGSQYASHD
FQRKLTSYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHGTRYRTHHEAVADLFEYIEVFYNRSRRHSSLGFMSPTQFMQNWLAAQRTKDTAA
AWIDTQCRQYPLSVLCEVLAVSINGYRAWKRGGTPERQRLTDAQLLTLIRTIHAKVKGAYGSPRMTDEVRSRGFPASKARVERLMSANGIRARHKRRYRV
TTDSRHKLPVAPNVLNRDFTPAEPNQVFTSDITYIWTDEGWLYLAVTLDLFNREVVGWSVKPRMTADLVTDALTMAWFRRRPAPGTLHHSDRGSQYASHD
FQRKLTSYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHGTRYRTHHEAVADLFEYIEVFYNRSRRHSSLGFMSPTQFMQNWLAAQRTKDTAA
Blast result :
Comments
Genome in progress.
ISBph1 is 88% (ORFA) and 93% (ORFB) aa similar to ISRso8. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISBph1 is 88% (ORFA) and 93% (ORFB) aa similar to ISRso8. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct Sibmission GenBank