ISBxe1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007951 | ND | Burkholderia xenovorans | Burkholderia xenovorans LB400 |
DNA section
IS Length : 1282 bp
Ends
IR Length : 36/49
IRL : TGAGCTTGCCCGCAAAAAATGGATCCCTTGCTGAGTGTGTAAAACTCACG
IRR : TGACGTTGCCCGCGAATTTCGGATCCCTTAGCTGAGTGAGATTATGCCAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACCCGGATT | ACT | GCCGCTACGC | 3 |
DNA sequence
TGAGCTTGCCCGCAAAAAATGGATCCCTTGCTGAGTGTGTAAAACTCACGCAAGGAGGAAGGAAAGATGGGTAATCCGAGAGCCCGATATACGCGGGAAT
TCATGCTGGAAGCCGTGCGCATGGTCCGCGGCGGCCAGAGCATGGCGGCGGTGGCGAAGATACTGGGTATCAGCCCGAAGACGCTGCACAACTGGGTTAA
GGCCGATGCCGCTGGGAAGCTGAACGGCGCAGGCAAACAGGTTTCTCCTGAACAGATGGAGATTGCCCGGCTGCGCGCGGAGTTGGCACGCGTGAAAATG
GAGCGCGACATATTGGAAAAAGCCACGGCGTACTTTGCAAAGGTGTCGGCATGAAGTACGCCTGGATCGAGCTTCACAGCCGACAATGGCCGGTGTCCCT
GACCTGCCAGGTGCTGGGTGTCAGCCCCAGCGGTTACCACGCGCGCAAGGCGCGGGATGTCGATACTGACCGAGCGCGCCGACGCATCAGCAACGACGCT
CTGCTGGTGCACATCAAGGCCGTGCACGCTGAATCCAAAGGCGAGTACGGCTGGCCGCGCGTGTGGAAGAAGTTGCTGGCCCAGGGCATTCGCGTCAGCA
AGGATCGTGTCCAACAGCTCATGAAGCTGCACGGCATCAGGGCGAAGACCAAACGCCGGTTCAAGGTCACGACCGACAGCAACCACAGCCTGCCGGTCGC
GCCGGACCTGCTGCAACGAGACTTCTCTCCCGCGCGTCCAGATCAGGTCTGGACTACGGACATCACGTACATCTGGACGGACGAGGGTTGGCTGTTTCTG
ACCGTCATCCTCGACCTGTTCAGCCGTCAGGTGGTGGGCTGGTCGATGCAGCCGCACATGCGCACGGAGCTGGTGTCTGACGCGCTGCGTATGGCGTGGT
TTCGCCGCCGGCCGGAAGCGGGCCTGATCGTCCATAGCGACCGCGGTAGCCAGTATTGCAGTCGTGACTTCCAGGACCTGCTTAAGGGCTACGGCATGCG
CAGCTCGATGAGCCGTCGAGGAAATTGTTGGGACAACGCGCCGACCGAGAGCTTGTGGGGATCGCTCAAGCGAGCACGCATCCTTGGCCAGCGCTTTGCA
ACGCGTCGGGAAGCGATGGACGAGGTAATCGACTGGTTGAGCTTCTACAATCATTCGCGCTTGCACTCGACGTTGGGCTACGTCAGTCCGATGCAGTTCG
AGCGGGACTGGTACGCTGCCCAGAACCAACGGGTGGCATAATCTCACTCAGCTAAGGGATCCGAAATTCGCGGGCAACGTCA
TCATGCTGGAAGCCGTGCGCATGGTCCGCGGCGGCCAGAGCATGGCGGCGGTGGCGAAGATACTGGGTATCAGCCCGAAGACGCTGCACAACTGGGTTAA
GGCCGATGCCGCTGGGAAGCTGAACGGCGCAGGCAAACAGGTTTCTCCTGAACAGATGGAGATTGCCCGGCTGCGCGCGGAGTTGGCACGCGTGAAAATG
GAGCGCGACATATTGGAAAAAGCCACGGCGTACTTTGCAAAGGTGTCGGCATGAAGTACGCCTGGATCGAGCTTCACAGCCGACAATGGCCGGTGTCCCT
GACCTGCCAGGTGCTGGGTGTCAGCCCCAGCGGTTACCACGCGCGCAAGGCGCGGGATGTCGATACTGACCGAGCGCGCCGACGCATCAGCAACGACGCT
CTGCTGGTGCACATCAAGGCCGTGCACGCTGAATCCAAAGGCGAGTACGGCTGGCCGCGCGTGTGGAAGAAGTTGCTGGCCCAGGGCATTCGCGTCAGCA
AGGATCGTGTCCAACAGCTCATGAAGCTGCACGGCATCAGGGCGAAGACCAAACGCCGGTTCAAGGTCACGACCGACAGCAACCACAGCCTGCCGGTCGC
GCCGGACCTGCTGCAACGAGACTTCTCTCCCGCGCGTCCAGATCAGGTCTGGACTACGGACATCACGTACATCTGGACGGACGAGGGTTGGCTGTTTCTG
ACCGTCATCCTCGACCTGTTCAGCCGTCAGGTGGTGGGCTGGTCGATGCAGCCGCACATGCGCACGGAGCTGGTGTCTGACGCGCTGCGTATGGCGTGGT
TTCGCCGCCGGCCGGAAGCGGGCCTGATCGTCCATAGCGACCGCGGTAGCCAGTATTGCAGTCGTGACTTCCAGGACCTGCTTAAGGGCTACGGCATGCG
CAGCTCGATGAGCCGTCGAGGAAATTGTTGGGACAACGCGCCGACCGAGAGCTTGTGGGGATCGCTCAAGCGAGCACGCATCCTTGGCCAGCGCTTTGCA
ACGCGTCGGGAAGCGATGGACGAGGTAATCGACTGGTTGAGCTTCTACAATCATTCGCGCTTGCACTCGACGTTGGGCTACGTCAGTCCGATGCAGTTCG
AGCGGGACTGGTACGCTGCCCAGAACCAACGGGTGGCATAATCTCACTCAGCTAAGGGATCCGAAATTCGCGGGCAACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 95 aa | 67 | 351 | + | No |
Description : First part of the transposase
ORF sequence :
MGNPRARYTREFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVKADAAGKLNGAGKQVSPEQMEIARLRAELARVKMERDILEKATAYFAKVSA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 990 bp | 329 aa | 252 | 1241 | + | No |
Description : Second part of the transposase
ORF sequence :
TDGDCPAARGVGTRENGARHIGKSHGVLCKGVGMKYAWIELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYG
WPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQ
PHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLKRARILGQRFATRREAMDEVIDWLSFYN
HSRLHSTLGYVSPMQFERDWYAAQNQRVA
WPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFKVTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQ
PHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSRDFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLKRARILGQRFATRREAMDEVIDWLSFYN
HSRLHSTLGYVSPMQFERDWYAAQNQRVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1175 bp | 391 aa | 67 | 1241 | + | Yes |
Chemistry : DDE
ORF sequence :
MGNPRARYTREFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVKADAAGKLNGAGKQVSPEQMEIARLRAELARVKMERDILEKSHGVLCKGVGMKYAW
IELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYGWPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFK
VTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQPHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSR
DFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLKRARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA
IELHSRQWPVSLTCQVLGVSPSGYHARKARDVDTDRARRRISNDALLVHIKAVHAESKGEYGWPRVWKKLLAQGIRVSKDRVQQLMKLHGIRAKTKRRFK
VTTDSNHSLPVAPDLLQRDFSPARPDQVWTTDITYIWTDEGWLFLTVILDLFSRQVVGWSMQPHMRTELVSDALRMAWFRRRPEAGLIVHSDRGSQYCSR
DFQDLLKGYGMRSSMSRRGNCWDNAPTESLWGSLKRARILGQRFATRREAMDEVIDWLSFYNHSRLHSTLGYVSPMQFERDWYAAQNQRVA
Blast result :
Comments
ISBxe1 is 78% (ORFA) aa similar to ISBcen17 and 73% (ORFB) aa similar to ISRso8.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Lykidis,A. and Richardson,P. (2006) Direct submission GenBank