ISBps1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAHR02000025 | ND | Burkholderia pseudomallei | Burkholderia pseudomallei 1655 |
DNA section
IS Length : 1290 bp
Ends
IR Length : 29/41
IRL : TGAGCTTCCCCTGAAATTTCGCTTTCCAGCAGGTCGTGTGTAGACTCGAC
IRR : TGACCTTCCCTCTGTTTTTCGCCTTCCAGAGGCCCCGGGTTATGCAACCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAGCACGGTA | CGCTGTCGTC | 0 |
DNA sequence
TGAGCTTCCCCTGAAATTTCGCTTTCCAGCAGGTCGTGTGTAGACTCGACCCCAAGGAAGGAAAGAAGAAATGTTCAAGGTATCAAAGCAGGCATACATG
GCGGAGTTCAAGGCGGCGGCCGTGCAGCGAGTCAAGGATGGGCAAGGCGTGGGCGTGGTGGCCCGCGAGTTGGGCGTGTCGGAGCAGACGGTGCGCAACT
GGGTGAAGGCTGAGCAGGCCGGCACGCTTAACGGTGCCGGCGCGAAGGTGGTCACGCCGGAGCAGATGGAACTCTCGCGCTTGCGTGCCGAGAACAAGCG
CCTGCAAATGGAGCTGGAAATCGCAAAAAAGGCGGCGGCGTTCTTCGCGAAGGACCTCCTGTGAAGTACGCCTGGATTGACGAGCAGTGCCGGCATTATC
CGTTGTCGGCGCTGTGCGAGGTCCTTGCCGTGAGCATCAACGGCTATCGTGCCTGGAAGCGCGGCGGCACGGTCCAGCGTAAGCGGCTCACCGACGCCCA
ATTGCTCACGTTGATTCGAACGATTCACGCCGAGGTCAAGGGCGCCTATGGCTCGCCGCGCATGACCGAGGAGATTCGCGCACGCGGGTTTCCGGCGAGC
AAAGCGCGCGTGGAGCGTCTGATGAGCAAGAACGGCATCCGGGCGCGCCACAAGCGCCGGTACCGCGTCACGACCGATTCCAAGCACAAGCTGCCGGTCG
CGCCGAACCTGCTGAATCGCGACTTCACGCCGGACGCGCCGAATCAGGTGTTTAGCTCGGACATCACGTACATCTGGACCGACGAAGGATGGCTGTACCT
GGCGGTCGTGCTGGACTTGTTCAATCGGGAAGTGGTGGGCTGGTCCATCAAGCCGCGCATGACGGCAGACCTTGTTACTGACGCACTTATGATGGCGTGG
TTCCGCCGGAAGCCCGCGCCCGGCGCACTGCATCACTCGGACCGTGGCAGCCAGTACGCGAGCCACGAGTTTCAGCGCAAGCTGGCCGACTATGGCATGC
ACTGCTCGATGAGCCGCAAGGGAAATTGTTGGGACAATGCGCCGACGGAGAGCTTCTTCAACAGCCTGAAGAACGAACGGGTACATGCTACGCACTACCG
GACGCACCGCGAGGCCGCAGCAGACTTGTTCGAATACATCGAAGTGTTTTATAGCCGCAGTCGTCGTCATTCGTCACTCGGCTTTGTACCGCCGACCCAG
TTCCTGCAAGACTGGCTCACTGCTCAGCAGGTAAAGGATGCGGTTGCATAACCCGGGGCCTCTGGAAGGCGAAAAACAGAGGGAAGGTCA
GCGGAGTTCAAGGCGGCGGCCGTGCAGCGAGTCAAGGATGGGCAAGGCGTGGGCGTGGTGGCCCGCGAGTTGGGCGTGTCGGAGCAGACGGTGCGCAACT
GGGTGAAGGCTGAGCAGGCCGGCACGCTTAACGGTGCCGGCGCGAAGGTGGTCACGCCGGAGCAGATGGAACTCTCGCGCTTGCGTGCCGAGAACAAGCG
CCTGCAAATGGAGCTGGAAATCGCAAAAAAGGCGGCGGCGTTCTTCGCGAAGGACCTCCTGTGAAGTACGCCTGGATTGACGAGCAGTGCCGGCATTATC
CGTTGTCGGCGCTGTGCGAGGTCCTTGCCGTGAGCATCAACGGCTATCGTGCCTGGAAGCGCGGCGGCACGGTCCAGCGTAAGCGGCTCACCGACGCCCA
ATTGCTCACGTTGATTCGAACGATTCACGCCGAGGTCAAGGGCGCCTATGGCTCGCCGCGCATGACCGAGGAGATTCGCGCACGCGGGTTTCCGGCGAGC
AAAGCGCGCGTGGAGCGTCTGATGAGCAAGAACGGCATCCGGGCGCGCCACAAGCGCCGGTACCGCGTCACGACCGATTCCAAGCACAAGCTGCCGGTCG
CGCCGAACCTGCTGAATCGCGACTTCACGCCGGACGCGCCGAATCAGGTGTTTAGCTCGGACATCACGTACATCTGGACCGACGAAGGATGGCTGTACCT
GGCGGTCGTGCTGGACTTGTTCAATCGGGAAGTGGTGGGCTGGTCCATCAAGCCGCGCATGACGGCAGACCTTGTTACTGACGCACTTATGATGGCGTGG
TTCCGCCGGAAGCCCGCGCCCGGCGCACTGCATCACTCGGACCGTGGCAGCCAGTACGCGAGCCACGAGTTTCAGCGCAAGCTGGCCGACTATGGCATGC
ACTGCTCGATGAGCCGCAAGGGAAATTGTTGGGACAATGCGCCGACGGAGAGCTTCTTCAACAGCCTGAAGAACGAACGGGTACATGCTACGCACTACCG
GACGCACCGCGAGGCCGCAGCAGACTTGTTCGAATACATCGAAGTGTTTTATAGCCGCAGTCGTCGTCATTCGTCACTCGGCTTTGTACCGCCGACCCAG
TTCCTGCAAGACTGGCTCACTGCTCAGCAGGTAAAGGATGCGGTTGCATAACCCGGGGCCTCTGGAAGGCGAAAAACAGAGGGAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 98 | 364 | + | No |
Description : First part of the transposase
ORF sequence :
MAEFKAAAVQRVKDGQGVGVVARELGVSEQTVRNWVKAEQAGTLNGAGAKVVTPEQMELSRLRAENKRLQMELEIAKKAAAFFAKDLL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1020 bp | 339 aa | 232 | 1251 | + | No |
Description : Second part of the transposase
ORF sequence :
RCRREGGHAGADGTLALACREQAPANGAGNRKKGGGVLREGPPVKYAWIDEQCRHYPLSALCEVLAVSINGYRAWKRGGTVQRKRLTDAQLLTLIRTIHA
EVKGAYGSPRMTEEIRARGFPASKARVERLMSKNGIRARHKRRYRVTTDSKHKLPVAPNLLNRDFTPDAPNQVFSSDITYIWTDEGWLYLAVVLDLFNRE
VVGWSIKPRMTADLVTDALMMAWFRRKPAPGALHHSDRGSQYASHEFQRKLADYGMHCSMSRKGNCWDNAPTESFFNSLKNERVHATHYRTHREAAADLF
EYIEVFYSRSRRHSSLGFVPPTQFLQDWLTAQQVKDAVA
EVKGAYGSPRMTEEIRARGFPASKARVERLMSKNGIRARHKRRYRVTTDSKHKLPVAPNLLNRDFTPDAPNQVFSSDITYIWTDEGWLYLAVVLDLFNRE
VVGWSIKPRMTADLVTDALMMAWFRRKPAPGALHHSDRGSQYASHEFQRKLADYGMHCSMSRKGNCWDNAPTESFFNSLKNERVHATHYRTHREAAADLF
EYIEVFYSRSRRHSSLGFVPPTQFLQDWLTAQQVKDAVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 384 aa | 98 | 1251 | + | Yes |
Chemistry : DDE
ORF sequence :
MAEFKAAAVQRVKDGQGVGVVARELGVSEQTVRNWVKAEQAGTLNGAGAKVVTPEQMELSRLRAENKRLQMELEIAKKGGGVLREGPPVKYAWIDEQCRH
YPLSALCEVLAVSINGYRAWKRGGTVQRKRLTDAQLLTLIRTIHAEVKGAYGSPRMTEEIRARGFPASKARVERLMSKNGIRARHKRRYRVTTDSKHKLP
VAPNLLNRDFTPDAPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIKPRMTADLVTDALMMAWFRRKPAPGALHHSDRGSQYASHEFQRKLADYG
MHCSMSRKGNCWDNAPTESFFNSLKNERVHATHYRTHREAAADLFEYIEVFYSRSRRHSSLGFVPPTQFLQDWLTAQQVKDAVA
YPLSALCEVLAVSINGYRAWKRGGTVQRKRLTDAQLLTLIRTIHAEVKGAYGSPRMTEEIRARGFPASKARVERLMSKNGIRARHKRRYRVTTDSKHKLP
VAPNLLNRDFTPDAPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIKPRMTADLVTDALMMAWFRRKPAPGALHHSDRGSQYASHEFQRKLADYG
MHCSMSRKGNCWDNAPTESFFNSLKNERVHATHYRTHREAAADLFEYIEVFYSRSRRHSSLGFVPPTQFLQDWLTAQQVKDAVA
Blast result :
Comments
Genome in progress.
ISBps1 is 95% (ORFA) and 98% (ORFB) aa similar to ISRso8. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
ISBps1 is 95% (ORFA) and 98% (ORFB) aa similar to ISRso8. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] NCBI Microbial Genomes Annotation Project. (2006) Direct submission GenBank