ISCce3

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAVC01000059 ND Clostridium cellulolyticum
Clostridium cellulolyticum H10
DNA section
IS Length : 1289 bp

Ends


IR Length : 31/45

IRL : TGACCTTACCCCAATTTGATGGACACACCGAATGGCTGGTATAATAAAAC
IRR : TGACCTTTCCCTGATAAATTAGACACAACGAATGGCGGGATTTCTCCGTT

Insertion site


Left flankDirect repeatRight flankDR Length
AAATGGATATAATTCAAATTCAC3

DNA sequence

TGACCTTACCCCAATTTGATGGACACACCGAATGGCTGGTATAATAAAACTAGTCGGAGGTACCCATCATGAAACGTTATGATAATAGTTTTAAAGAACA
AGCGGTCCGTCTTGTTACAGAGCAAGGTAAAAGTGTATCAATTGTGGCTAAAGACATTGGAATCCACGAAAACACTCTATACAAATGGATTGACCAGTAC
AAAACTCATAAAGAGAATGCTTTCCCAGGTAGCGGCAACCTAAGACCTGAAGATGAAGAACTCAGGAAACTTAAGAAGCGTGTTGCAGACCTTGAAATGG
AGAATGAATTACTAAAAAAAGTAACGGCAATCTTCGCGAAAGACCGGAAATAATATATCCTATCATTCACAAATACCGCTTCAAATTTCCTGTTCAGAAG
ATGTGCCAAATACTAAAAATATCAAGAAGCGGTTATTATGCTTGGATTAAAAGGCCTGAAAGCTTGCGCAAGAAAAGGAATGTGGAACTTCTTGAAAAAA
TCAGAAACATTCACAAAGTATCCCGTGAAACCTATGGAAGCCCCCGGATAACCAAGGCTTTAAAGAATGAAGGCATAAACTGTGGTAAAAATCGTATAGC
TAAACTGATGAAGGAAAATAATATTACTGCTAAAACTAGGAAGAAGTTTAAGGCTACAACTAATTCAAAGCACAATTACCCGGTTGCAGATAATATCCTA
AATCAGGACTTTACAGCTTCTAGGCCTAATCAAATCTGGGTTGCAGATATTACTTATATTCCTACAGACGAGGGTTGGCTCTATTTGGCTGCCATTGTTG
ATCTCTACAACAGAAAAGTTGTTGGATGGGCAATGGATATCACTATGACAAAACAGCTTTGTATAGATGCTTTAAAGCAAGCCATCGGCAGGCAGAGACC
ATCAAATGGAGTGATTCATCATTCCGACCGAGGGGTCCAATATGCCAGCAAGGAATATCAGAAAGTACTGAAAAGCAATGGTTTTACAGCAAGTATGAGT
CGCAAGGGTAACTGTTACGATAATGCCTGTATGGAATCATTCTTTGGAACTCTCAAAACGGAGCTTATTTACTTAACAAGGTTCAAAACAAGGACTGAGG
CCCGTCTGGCTATCTTTGAGTATATTGAAGTCTTTTATAATCGGATACGATTACATTCAAAGCTTGGCTATAAGTCGCCTGTGGAATTTGAAAAGCAAAA
CAAAGCTGCTTAAATAAAAGTTAATAAGATACAAAGGAAAACGGAGAAATCCCGCCATTCGTTGTGTCTAATTTATCAGGGAAAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
285 bp94 aa69353+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKRYDNSFKEQAVRLVTEQGKSVSIVAKDIGIHENTLYKWIDQYKTHKENAFPGSGNLRPEDEELRKLKKRVADLEMENELLKKVTAIFAKDRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
906 bp301 aa3081213+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ITKKSNGNLRERPEIIYPIIHKYRFKFPVQKMCQILKISRSGYYAWIKRPESLRKKRNVELLEKIRNIHKVSRETYGSPRITKALKNEGINCGKNRIAKL
MKENNITAKTRKKFKATTNSKHNYPVADNILNQDFTASRPNQIWVADITYIPTDEGWLYLAAIVDLYNRKVVGWAMDITMTKQLCIDALKQAIGRQRPSN
GVIHHSDRGVQYASKEYQKVLKSNGFTASMSRKGNCYDNACMESFFGTLKTELIYLTRFKTRTEARLAIFEYIEVFYNRIRLHSKLGYKSPVEFEKQNKA
A

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1145 bp384 aa691213+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRYDNSFKEQAVRLVTEQGKSVSIVAKDIGIHENTLYKWIDQYKTHKENAFPGSGNLRPEDEELRKLKKRVADLEMENELLKITKKSNGNLRERPEIIY
PIIHKYRFKFPVQKMCQILKISRSGYYAWIKRPESLRKKRNVELLEKIRNIHKVSRETYGSPRITKALKNEGINCGKNRIAKLMKENNITAKTRKKFKAT
TNSKHNYPVADNILNQDFTASRPNQIWVADITYIPTDEGWLYLAAIVDLYNRKVVGWAMDITMTKQLCIDALKQAIGRQRPSNGVIHHSDRGVQYASKEY
QKVLKSNGFTASMSRKGNCYDNACMESFFGTLKTELIYLTRFKTRTEARLAIFEYIEVFYNRIRLHSKLGYKSPVEFEKQNKAA

 

Blast result :
Comments
Genome in progress.
ISCce3 is 63% % (ORFA)aa similar to IS1076 and 64% (ORFB) aa similar to ISPa20 The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct Submission GenBank