ISCb3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AALO01000009 | ND | Clostridium beijerincki | Clostridium beijerincki NCIMB 8052 |
DNA section
IS Length : 1246 bp
Ends
IR Length : 22/37
IRL : TGGACACATTCTTGAAATAAATTATAATTAAAGTAAGAGAGGTGTGTCCG
IRR : TGGACACATTTTTATTAAATTACTTTCTTTACCAAATCCTCTTTCATTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAGTTTTA | AATTATTGAC | 0 |
DNA sequence
TGGACACATTCTTGAAATAAATTATAATTAAAGTAAGAGAGGTGTGTCCGAATGAGAAGGAAAAGTTATACAGAAGATTTTAAGAAAATGATAATTGAGG
TCTACAATACTGGAAAACCGATTAAAGAAATCTGTAAAGAATATGGCGTTTCAACAACATCACTTAATAACTGGATCAATCAAGTTCAGCCAAAAGAGAA
AATATCTAAGAAACCAATAATAACTAACAATAAAATTAGAAATAATGAAATAAAAGACAAAAAAGATGTTGAAGAGATTTCTAAGTTAAAAAAAGAAAAT
GAAAAGATTAGAATGGAACTTGAAATCTTAAAAAAAGCTATAGCCATATTCTCAAAAGACGAAGAGATATAATTAAATTTATTGATGATAATAAATCGAT
TTATCCAGTGAGATTGATGTGTAAAGTCTTACACATGCCTAAAAGCACTTACTATCAATCTCATCACTATACTCCGTCCCGAAGGACGTTGGAAAGTGAA
ACTCTAAAAGATCAAATACTTAAAATTTATACTGAAAGTAATCGTAGGTATGGTGCGCCCAAAATTCAAAAAATACTTGAACAATCAGGACATAAAATTA
GTATTAAAAGAGTTCAACGTTTTATGAAGCAGTTAAATATATATTCGATAGTAATTAAGAAATTTAGACCTGCAAAAGCTAATAGAAAAGTTATAGAACG
AAAAAATCTCCTTAAGCAAGATTTTACTACTAATAAAATAAACGAGAAATGGGTTGGAGACATAACTTATATCTATACCCTAAAAGATGGATGGTGCTAT
TTGGCATCTGTTATGGATTTATGTACAAGAAAGATAATTGGATATTCATTTTCAAAAACAATGGATGCTTCCGTAGCGGTAGCTGCTTTAAATAATGCGT
ATACACTTCAACAGCCTGTCGGCTCCGTTATTTTTCACTCTGACCTTGGAGTACAATATACGAGCACAGATTTTCTTAATAGATTAAAAAAATACAAAAT
GAAAAGCTCTAATAGCAGAAAAGGATGCCCTTATGATAATGCCTGTATTGAGTCTTTTCATTCAATTTTGAAAAAAGAACAAGTGAACAATGTTCAATAC
TTGGATTTTGAAAGCGCAAAACTAGATTTATTTATATTTATCGAATCATGGTATAACCGCAAAAGAATTCACGGCTCTATTGGTTATATTACTCCACAAA
TGAAAGAGGATTTGGTAAAGAAAGTAATTTAATAAAAATGTGTCCA
TCTACAATACTGGAAAACCGATTAAAGAAATCTGTAAAGAATATGGCGTTTCAACAACATCACTTAATAACTGGATCAATCAAGTTCAGCCAAAAGAGAA
AATATCTAAGAAACCAATAATAACTAACAATAAAATTAGAAATAATGAAATAAAAGACAAAAAAGATGTTGAAGAGATTTCTAAGTTAAAAAAAGAAAAT
GAAAAGATTAGAATGGAACTTGAAATCTTAAAAAAAGCTATAGCCATATTCTCAAAAGACGAAGAGATATAATTAAATTTATTGATGATAATAAATCGAT
TTATCCAGTGAGATTGATGTGTAAAGTCTTACACATGCCTAAAAGCACTTACTATCAATCTCATCACTATACTCCGTCCCGAAGGACGTTGGAAAGTGAA
ACTCTAAAAGATCAAATACTTAAAATTTATACTGAAAGTAATCGTAGGTATGGTGCGCCCAAAATTCAAAAAATACTTGAACAATCAGGACATAAAATTA
GTATTAAAAGAGTTCAACGTTTTATGAAGCAGTTAAATATATATTCGATAGTAATTAAGAAATTTAGACCTGCAAAAGCTAATAGAAAAGTTATAGAACG
AAAAAATCTCCTTAAGCAAGATTTTACTACTAATAAAATAAACGAGAAATGGGTTGGAGACATAACTTATATCTATACCCTAAAAGATGGATGGTGCTAT
TTGGCATCTGTTATGGATTTATGTACAAGAAAGATAATTGGATATTCATTTTCAAAAACAATGGATGCTTCCGTAGCGGTAGCTGCTTTAAATAATGCGT
ATACACTTCAACAGCCTGTCGGCTCCGTTATTTTTCACTCTGACCTTGGAGTACAATATACGAGCACAGATTTTCTTAATAGATTAAAAAAATACAAAAT
GAAAAGCTCTAATAGCAGAAAAGGATGCCCTTATGATAATGCCTGTATTGAGTCTTTTCATTCAATTTTGAAAAAAGAACAAGTGAACAATGTTCAATAC
TTGGATTTTGAAAGCGCAAAACTAGATTTATTTATATTTATCGAATCATGGTATAACCGCAAAAGAATTCACGGCTCTATTGGTTATATTACTCCACAAA
TGAAAGAGGATTTGGTAAAGAAAGTAATTTAATAAAAATGTGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 106 aa | 52 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MRRKSYTEDFKKMIIEVYNTGKPIKEICKEYGVSTTSLNNWINQVQPKEKISKKPIITNNKIRNNEIKDKKDVEEISKLKKENEKIRMELEILKKAIAIF
SKDEEI
SKDEEI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 324 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
NLKKSYSHILKRRRDIIKFIDDNKSIYPVRLMCKVLHMPKSTYYQSHHYTPSRRTLESETLKDQILKIYTESNRRYGAPKIQKILEQSGHKISIKRVQRF
MKQLNIYSIVIKKFRPAKANRKVIERKNLLKQDFTTNKINEKWVGDITYIYTLKDGWCYLASVMDLCTRKIIGYSFSKTMDASVAVAALNNAYTLQQPVG
SVIFHSDLGVQYTSTDFLNRLKKYKMKSSNSRKGCPYDNACIESFHSILKKEQVNNVQYLDFESAKLDLFIFIESWYNRKRIHGSIGYITPQMKEDLVKK
VI
MKQLNIYSIVIKKFRPAKANRKVIERKNLLKQDFTTNKINEKWVGDITYIYTLKDGWCYLASVMDLCTRKIIGYSFSKTMDASVAVAALNNAYTLQQPVG
SVIFHSDLGVQYTSTDFLNRLKKYKMKSSNSRKGCPYDNACIESFHSILKKEQVNNVQYLDFESAKLDLFIFIESWYNRKRIHGSIGYITPQMKEDLVKK
VI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1181 bp | 393 aa | 52 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MRRKSYTEDFKKMIIEVYNTGKPIKEICKEYGVSTTSLNNWINQVQPKEKISKKPIITNNKIRNNEIKDKKDVEEISKLKKENEKIRMELEILKKSYSHI
LKRRRDIIKFIDDNKSIYPVRLMCKVLHMPKSTYYQSHHYTPSRRTLESETLKDQILKIYTESNRRYGAPKIQKILEQSGHKISIKRVQRFMKQLNIYSI
VIKKFRPAKANRKVIERKNLLKQDFTTNKINEKWVGDITYIYTLKDGWCYLASVMDLCTRKIIGYSFSKTMDASVAVAALNNAYTLQQPVGSVIFHSDLG
VQYTSTDFLNRLKKYKMKSSNSRKGCPYDNACIESFHSILKKEQVNNVQYLDFESAKLDLFIFIESWYNRKRIHGSIGYITPQMKEDLVKKVI
LKRRRDIIKFIDDNKSIYPVRLMCKVLHMPKSTYYQSHHYTPSRRTLESETLKDQILKIYTESNRRYGAPKIQKILEQSGHKISIKRVQRFMKQLNIYSI
VIKKFRPAKANRKVIERKNLLKQDFTTNKINEKWVGDITYIYTLKDGWCYLASVMDLCTRKIIGYSFSKTMDASVAVAALNNAYTLQQPVGSVIFHSDLG
VQYTSTDFLNRLKKYKMKSSNSRKGCPYDNACIESFHSILKKEQVNNVQYLDFESAKLDLFIFIESWYNRKRIHGSIGYITPQMKEDLVKKVI
Blast result :
Comments
Genome in progress.
ISCb3 is 58% (ORFA) aa similar to IS655 and 61% (ORFB) aa similar to IS981.
The third ORF is a putative ORF AB transposase reconstructed in sillico by -1 frameshift.
ISCb3 is 58% (ORFA) aa similar to IS655 and 61% (ORFB) aa similar to IS981.
The third ORF is a putative ORF AB transposase reconstructed in sillico by -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank