ISCausp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AATH01000002 | ND | Caulobacter sp. | Caulobacter sp. K31 |
DNA section
IS Length : 1262 bp
Ends
IR Length : 18/24
IRL : TGATCCTCCCTCGTTTCAGTGGACACCCATGCTAGCTTTTCGGAGCTGGA
IRR : TGATCTTCCCCCGAGAAAGTGGACGGGGTTAAGCCGCTCTGCGTTCCATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGAGGTCGTC | GGGTGAAATC | 0 |
DNA sequence
TGATCCTCCCTCGTTTCAGTGGACACCCATGCTAGCTTTTCGGAGCTGGAGAGGAGTGTGCGATGAGCGTGAAGCGTCGGGTCTTTCCGGAGACCTTCAA
ACGAGAAGCGGTTGACCGTGTCGCCAGCAGCGGCCTGTCAGCCGGCGCAGTAGCCCGGGAGTTGGGCCTGCACGAGACGGTCCTGCGACGATGGATGATG
CAATACGGGACGCAGGCGACGGGGCCGGCGAGGCGCCCCCACACGCAAGCGCCGGCCCCGTCGCCGTCTGACCTGGTCGCCGAGAACGCCCGCCTGCGGC
GTGAGAACGACCATCTGCGCATGGAGCGAGACATCTTAAAAAAAGCCGCGCTCATCTTCGGAGCGGCCTCCCGATGAAGTTCGGGTTCGTCGATGAGCAC
CGCGGCGTCTGGCCGGTTCGAATGATGTGTCGGGTCCTGGGCCTGTCGGCCAGCGGCTACTACGCTTGGCGCGTCAGGCCCGAGGGCCGGCGGGCGGCGG
CCAACCGCGCCCTGATCGAGGACATCCGCCTGATCCATGCCGAGAGCTGCGGAACCTACGGTTCGCCGCGCGTCCACGCCGTCTTGCGCGGCCATGGCCG
CCGTATCGGACGCTCGCGGGTCGAGCGGCTGATGCGCCGCGCCGGCCTGCGCGGCCTGGCCGCTTTGCCCCGCCGCACCCGGACGACCGACAGCCGCCAT
GGCTATCCGATCGCCCCCAATAGGCTCGCCCGTAACTTCCAGGCGGCGGCCCCTGGCCAGGTCTGGCTCGCCGACCTGACCTATATTCCGACCGGGGAAG
GCTGGCTCTATCTGGCCGGCATCCTCGACATGCACACCCGCAAGCTCGTCGGCTGGTCGATGCGCGACACCTTGCACGCCCGGATCGCCCTGGAGGCCCT
GACCATGGCCATCGAGCGCCAAAAGCCCGCGCCCGGACTCATCCATCATTCGGATCGCGGCATTCAATACGCCGCCGAGACCTACCGCTCGGCCCTGGCC
CAGTCAGGGATCACCCCATCCATGAGCCGCAAGGGCGACTGCTGGGACAACGCCCCGATGGAAAGTTTCTTCCACACCCTCAAGACCGAGCGGGTCCATC
ACCGGATCTACGCCACCCGCGCCGAGGCCCGGCGCGATCTGTTCGGCTATATCGAGGGCTTCTACAATTCACGTCGCCTGCACTCGGCTCTGGGCTATAT
CAGTCCCGCCGAGATGGAACGCAGAGCGGCTTAACCCCGTCCACTTTCTCGGGGGAAGATCA
ACGAGAAGCGGTTGACCGTGTCGCCAGCAGCGGCCTGTCAGCCGGCGCAGTAGCCCGGGAGTTGGGCCTGCACGAGACGGTCCTGCGACGATGGATGATG
CAATACGGGACGCAGGCGACGGGGCCGGCGAGGCGCCCCCACACGCAAGCGCCGGCCCCGTCGCCGTCTGACCTGGTCGCCGAGAACGCCCGCCTGCGGC
GTGAGAACGACCATCTGCGCATGGAGCGAGACATCTTAAAAAAAGCCGCGCTCATCTTCGGAGCGGCCTCCCGATGAAGTTCGGGTTCGTCGATGAGCAC
CGCGGCGTCTGGCCGGTTCGAATGATGTGTCGGGTCCTGGGCCTGTCGGCCAGCGGCTACTACGCTTGGCGCGTCAGGCCCGAGGGCCGGCGGGCGGCGG
CCAACCGCGCCCTGATCGAGGACATCCGCCTGATCCATGCCGAGAGCTGCGGAACCTACGGTTCGCCGCGCGTCCACGCCGTCTTGCGCGGCCATGGCCG
CCGTATCGGACGCTCGCGGGTCGAGCGGCTGATGCGCCGCGCCGGCCTGCGCGGCCTGGCCGCTTTGCCCCGCCGCACCCGGACGACCGACAGCCGCCAT
GGCTATCCGATCGCCCCCAATAGGCTCGCCCGTAACTTCCAGGCGGCGGCCCCTGGCCAGGTCTGGCTCGCCGACCTGACCTATATTCCGACCGGGGAAG
GCTGGCTCTATCTGGCCGGCATCCTCGACATGCACACCCGCAAGCTCGTCGGCTGGTCGATGCGCGACACCTTGCACGCCCGGATCGCCCTGGAGGCCCT
GACCATGGCCATCGAGCGCCAAAAGCCCGCGCCCGGACTCATCCATCATTCGGATCGCGGCATTCAATACGCCGCCGAGACCTACCGCTCGGCCCTGGCC
CAGTCAGGGATCACCCCATCCATGAGCCGCAAGGGCGACTGCTGGGACAACGCCCCGATGGAAAGTTTCTTCCACACCCTCAAGACCGAGCGGGTCCATC
ACCGGATCTACGCCACCCGCGCCGAGGCCCGGCGCGATCTGTTCGGCTATATCGAGGGCTTCTACAATTCACGTCGCCTGCACTCGGCTCTGGGCTATAT
CAGTCCCGCCGAGATGGAACGCAGAGCGGCTTAACCCCGTCCACTTTCTCGGGGGAAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 315 bp | 104 aa | 63 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MSVKRRVFPETFKREAVDRVASSGLSAGVVARELGLHETVLRRWMIQYGTRATGPARRPHTQAPAPSPSDLAAENARLRRENDHLRMERDILKKAALIFG
AASR
AASR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 305 | 1234 | + | No |
Description : Second part of the transposase
ORF sequence :
ERPSAHGARHLKKSRAHLRSGLPMKFGFVDEHRGVWPVRMMCRVLGLSASGYYAWRVRPESRRAAANRALIEDIRLIHAESCGTYGSPRVHAVLRGHGRR
VGRSRVERLMRRAGLRGLAALPRRTRTTDSRHGYPIAPNRLARHFQAAAPGQVWLADLTYIPTGEGWLYLAGILDMHTRKLVGWSMRDTLHAQIALDALT
MAIARQKPAPGLIHHSDRGIQYAAETYRSALARSGITPSMSRKGDCWDNAPMESFFHTFKTERVHHRIYATRAEARRDLFGYIEGFYNSRRLHSALGYIS
PAEAERRAA
VGRSRVERLMRRAGLRGLAALPRRTRTTDSRHGYPIAPNRLARHFQAAAPGQVWLADLTYIPTGEGWLYLAGILDMHTRKLVGWSMRDTLHAQIALDALT
MAIARQKPAPGLIHHSDRGIQYAAETYRSALARSGITPSMSRKGDCWDNAPMESFFHTFKTERVHHRIYATRAEARRDLFGYIEGFYNSRRLHSALGYIS
PAEAERRAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 63 | 1234 | + | Yes |
Chemistry : DDE
ORF sequence :
MSVKRRVFPETFKREAVDRVASSGLSAGVVARELGLHETVLRRWMIQYGTRATGPARRPHTQAPAPSPSDLAAENARLRRENDHLRMERDILKKSRAHLR
SGLPMKFGFVDEHRGVWPVRMMCRVLGLSASGYYAWRVRPESRRAAANRALIEDIRLIHAESCGTYGSPRVHAVLRGHGRRVGRSRVERLMRRAGLRGLA
ALPRRTRTTDSRHGYPIAPNRLARHFQAAAPGQVWLADLTYIPTGEGWLYLAGILDMHTRKLVGWSMRDTLHAQIALDALTMAIARQKPAPGLIHHSDRG
IQYAAETYRSALARSGITPSMSRKGDCWDNAPMESFFHTFKTERVHHRIYATRAEARRDLFGYIEGFYNSRRLHSALGYISPAEAERRAA
SGLPMKFGFVDEHRGVWPVRMMCRVLGLSASGYYAWRVRPESRRAAANRALIEDIRLIHAESCGTYGSPRVHAVLRGHGRRVGRSRVERLMRRAGLRGLA
ALPRRTRTTDSRHGYPIAPNRLARHFQAAAPGQVWLADLTYIPTGEGWLYLAGILDMHTRKLVGWSMRDTLHAQIALDALTMAIARQKPAPGLIHHSDRG
IQYAAETYRSALARSGITPSMSRKGDCWDNAPMESFFHTFKTERVHHRIYATRAEARRDLFGYIEGFYNSRRLHSALGYISPAEAERRAA
Blast result :
Comments
Genome in progress.
ISCausp1 is 56% (ORFA) aa similar to IS1953 and 66% (ORFB) aa similar to ISRso8. The third ORF is a putative OrfAB transposase reconstructed in sillico by -1 frameshift.
ISCausp1 is 56% (ORFA) aa similar to IS1953 and 66% (ORFB) aa similar to ISRso8. The third ORF is a putative OrfAB transposase reconstructed in sillico by -1 frameshift.
References
1] Larimer,F., Land,M. and Hauser,L. (2006) Direct Submission GenBank