ISApr1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AATR01000012 | ND | Alpha proteobacterium | Alpha proteobacterium HTCC2255 |
DNA section
IS Length : 1260 bp
Ends
IR Length : 29/44
IRL : TGAGGTGGTAGATTTGATTCGGACACTCTAGATAAGAGATAATTCTCAAA
IRR : TGACGTGGTCAACATATTTCGGACAACCCAGTTAAGCAACTTTTTGTAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCTAAAAGC | CTA | GGCTTTAAAG | 3 |
DNA sequence
TGAGGTGGTAGATTTGATTCGGACACTCTAGATAAGAGATAATTCTCAAAACTGGAGTGCATTATGAATGCTAGAAAAAAATATTCAAAAGAATTTAAGT
TAGACGCTATCAGCCTAGTGGTTGATCAGAAGTTCAGTCGAGCTGAAGCGGCAAGAAGCTTGGAGATTAATCCAAATCTGATTACCCGTTGGATGAAAGA
ACACGAACAAGATAATGATGGTCAGGCGTTTCGTGGTAATGGCAAGCTGACGCCAGAGCAAGAAGAAATTAGGCAACTGAGAGCAAAGGTTCGCCAGCTT
GAAATGGACAAAAACATTTTAAAGGAAGCAACGGTCTTTTTTGCCAAAGAAATGAAGTAAAGTACCGCTTCATTGCCCAAAATAAGAAGACCTATCCCGT
TGACAGAATGTGTCAATTGTTAGGGGTGAAGCGCAGCGGCTTTTACCGTTGGGCGAAGATAGAACAAGAAATAGACCCTACTCATGCTGATATGATCGAG
TGGGTGAAAAAGATAGCTGTCGCTAGTGATAACACGTATGGCAGCCGCAGAATGAAGCATGCCCTGAATTGTTTAGGCTATCCAGTGAGTCGCCAAAAGG
CGAAGAAGCTAATGCTTGAAGCCAAGGTGTGGGTTCGTTATCGCAAGAAATATAAGGTGACAACAGACAGTAACCATAACAAGCCGTTGTTTGATAATGT
GCTCAATCGTGAGTTTACTGTGGATGCGCCTAACAAAGCGTATGTTTCTGATATTACTTACATATGGACGCGAGAAGGTTGGTTGTACTTAGCCATCGTG
ATTGATTTGTACTCGCGTAAAATCGTTGGTTGGAGCATGAGCTCAAGGATGAAAGCAAAGCTGGTAATTGACGCGCTGAGAATGGCAATATGGCAGCGAA
AACCAGAGAAAGGATTAATTGTGCATACCGACCGTGGCTCGCAATATGCCAGTCACGAGTATCGTAAATTACTTAACGCTCATGGCCTAGTTGGCAGCAT
GAGCAGAAAAGGCGATTGTTGGGATAATGCCGTTGCTGAAAGTTTCTTCGGCAGCTTGAAAAACGAACGGGTTCATTGGCGAAACTACCAAACTCGATAC
GAAGCACAACAGGATATCTTGAATTACATCACGATGTTCTATAACAGCTATAGACTGCATTCATATCTGGGGTATCAAAGCCCAAATCAGTATGAGCAGG
AAATGGTAAAACTACAAAAAGTTGCTTAACTGGGTTGTCCGAAATATGTTGACCACGTCA
TAGACGCTATCAGCCTAGTGGTTGATCAGAAGTTCAGTCGAGCTGAAGCGGCAAGAAGCTTGGAGATTAATCCAAATCTGATTACCCGTTGGATGAAAGA
ACACGAACAAGATAATGATGGTCAGGCGTTTCGTGGTAATGGCAAGCTGACGCCAGAGCAAGAAGAAATTAGGCAACTGAGAGCAAAGGTTCGCCAGCTT
GAAATGGACAAAAACATTTTAAAGGAAGCAACGGTCTTTTTTGCCAAAGAAATGAAGTAAAGTACCGCTTCATTGCCCAAAATAAGAAGACCTATCCCGT
TGACAGAATGTGTCAATTGTTAGGGGTGAAGCGCAGCGGCTTTTACCGTTGGGCGAAGATAGAACAAGAAATAGACCCTACTCATGCTGATATGATCGAG
TGGGTGAAAAAGATAGCTGTCGCTAGTGATAACACGTATGGCAGCCGCAGAATGAAGCATGCCCTGAATTGTTTAGGCTATCCAGTGAGTCGCCAAAAGG
CGAAGAAGCTAATGCTTGAAGCCAAGGTGTGGGTTCGTTATCGCAAGAAATATAAGGTGACAACAGACAGTAACCATAACAAGCCGTTGTTTGATAATGT
GCTCAATCGTGAGTTTACTGTGGATGCGCCTAACAAAGCGTATGTTTCTGATATTACTTACATATGGACGCGAGAAGGTTGGTTGTACTTAGCCATCGTG
ATTGATTTGTACTCGCGTAAAATCGTTGGTTGGAGCATGAGCTCAAGGATGAAAGCAAAGCTGGTAATTGACGCGCTGAGAATGGCAATATGGCAGCGAA
AACCAGAGAAAGGATTAATTGTGCATACCGACCGTGGCTCGCAATATGCCAGTCACGAGTATCGTAAATTACTTAACGCTCATGGCCTAGTTGGCAGCAT
GAGCAGAAAAGGCGATTGTTGGGATAATGCCGTTGCTGAAAGTTTCTTCGGCAGCTTGAAAAACGAACGGGTTCATTGGCGAAACTACCAAACTCGATAC
GAAGCACAACAGGATATCTTGAATTACATCACGATGTTCTATAACAGCTATAGACTGCATTCATATCTGGGGTATCAAAGCCCAAATCAGTATGAGCAGG
AAATGGTAAAACTACAAAAAGTTGCTTAACTGGGTTGTCCGAAATATGTTGACCACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 64 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MNARKKYSKEFKLDAISLVVDQKFSRAEAARSLEINPNLITRWMKEHEQDNDGQAFRGNGKLTPEQEEIRQLRAKVRQLEMDKNILKEATVFFAKEMK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 303 | 1229 | + | No |
Description : Second part of the transposase
ORF sequence :
NGQKHFKGSNGLFCQRNEVKYRFIAQNKKTYPVDRMCQLLGVKRSGFYRWAKIEQEIDPTHADMIEWVKKIAVASDNTYGSRRMKHALNCLGYPVSRQKA
KKLMLEAKVWVRYRKKYKVTTDSNHNKPLFDNVLNREFTVDAPNKAYVSDITYIWTREGWLYLAIVIDLYSRKIVGWSMSSRMKAKLVIDALRMAIWQRK
PEKGLIVHTDRGSQYASHEYRKLLNAHGLVGSMSRKGDCWDNAVAESFFGSLKNERVHWRNYQTRYEAQQDILNYITMFYNSYRLHSYLGYQSPNQYEQE
MVKLQKVA
KKLMLEAKVWVRYRKKYKVTTDSNHNKPLFDNVLNREFTVDAPNKAYVSDITYIWTREGWLYLAIVIDLYSRKIVGWSMSSRMKAKLVIDALRMAIWQRK
PEKGLIVHTDRGSQYASHEYRKLLNAHGLVGSMSRKGDCWDNAVAESFFGSLKNERVHWRNYQTRYEAQQDILNYITMFYNSYRLHSYLGYQSPNQYEQE
MVKLQKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 64 | 1229 | + | Yes |
Chemistry : DDE
ORF sequence :
MNARKKYSKEFKLDAISLVVDQKFSRAEAARSLEINPNLITRWMKEHEQDNDGQAFRGNGKLTPEQEEIRQLRAKVRQLEMDKKHFKGSNGLFCQRNEVK
YRFIAQNKKTYPVDRMCQLLGVKRSGFYRWAKIEQEIDPTHADMIEWVKKIAVASDNTYGSRRMKHALNCLGYPVSRQKAKKLMLEAKVWVRYRKKYKVT
TDSNHNKPLFDNVLNREFTVDAPNKAYVSDITYIWTREGWLYLAIVIDLYSRKIVGWSMSSRMKAKLVIDALRMAIWQRKPEKGLIVHTDRGSQYASHEY
RKLLNAHGLVGSMSRKGDCWDNAVAESFFGSLKNERVHWRNYQTRYEAQQDILNYITMFYNSYRLHSYLGYQSPNQYEQEMVKLQKVA
YRFIAQNKKTYPVDRMCQLLGVKRSGFYRWAKIEQEIDPTHADMIEWVKKIAVASDNTYGSRRMKHALNCLGYPVSRQKAKKLMLEAKVWVRYRKKYKVT
TDSNHNKPLFDNVLNREFTVDAPNKAYVSDITYIWTREGWLYLAIVIDLYSRKIVGWSMSSRMKAKLVIDALRMAIWQRKPEKGLIVHTDRGSQYASHEY
RKLLNAHGLVGSMSRKGDCWDNAVAESFFGSLKNERVHWRNYQTRYEAQQDILNYITMFYNSYRLHSYLGYQSPNQYEQEMVKLQKVA
Blast result :
Comments
ISApr1 is 68% (ORFA) aa similar to IS911 and 65% (ORFB) aa similar to ISRso8. The third ORF is a putative ORF AB transposase reconstructed in sillico by -1 frameshift.
References
1] Giovannoni,S., Cho,J.-C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.