ISSdy2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF501265 | ND | Streptococcus dysgalactiae | Streptococcus dysgalactiae subsp dysgalactiae |
DNA section
IS Length : 1218 bp
Ends
IR Length : 30/46
IRL : TGAATAGGTTACACTAAACTAGACAGAATTTATAAAGTGTTCTACACTAA
IRR : TGAAAAGGCAACACTTTTCTGTACAACATTTATAAAGTGTTTTCTAGGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAATTATGG | ATC | TCTATAACCA | 3 |
DNA sequence
TGAATAGGTTACACTAAACTAGACAGAATTTATAAAGTGTTCTACACTAAAGAAAATAGGAGAACAGATATGTCTAGAAAAATACGTCGCCACTTCACCG
ATGATTTTAAGCAACAAATTGTTGACCTTTACAATGCTGGTAGAAAGCGTAGCAGTCTCATCAAGGAATATGAGCTAACCCCTTCCACCTTCGATAAGTG
GGTTAGACAAGCCAAAACAACAGGTTCATTCAAGTCTGTTGATAATCTGACAGATGAACAGCGGGAGCTGATTGAACTCAGAAAATGCAATAAAGAACTC
GAAATGCAATTAGATATCCTAAAGCAAGCGGCGGTGATTATGGCACAAAAAGGGAAATAATCACTGCTAATAAGGATAACTATAGCATTTCAGCCATGTG
TCGCTGGCTGAATATGCCACGCTCAAGTTATTACTATCAAGCCGTGGAGTCAGTATCTGAAACGGAGTTTGAAGAAACTATTAAAAGAATTTTCCTCAAG
AGCAAGTCCAGATACGGATCCAGAAAAATCAAAATATGCTTGAATAACGAAGGTATTACACTTTCACGTCGTCGGATTCGACGCATTATGAAGCGACTCA
ATTTGGTTTCTGTTTATCAGAAAGCCACCTTCAAACCACATTACAGAGGCAAGAATGAAGCCCCTATTCCCAACCATTTAGACAGGCAATTTAAGCAAGA
AAGACCACTACAAGCCTTAGTCACTGACTTAACCTATGTTCGTTTAGGCAATCGTTGGGCTTATGTTTGCCTTATTATTGACCTATACAACCGTGAAATC
ATCGGCCTGTCTCTTGGTTGGCACAAAACCGCTGAACTCGTTAAGCAAGCCATTCAAAGCATCCCTTACGCCCTGACCAAAGTCAAGATGTTCCATTCAG
ATCGTGGCAAAGAGTTTGATAATCAGTTAATTGATGAAATATTGGAAGCCTTTGGAATCACACGTTCGCTTAGTCAGGCTGGTTGTCCTTATGACAATGC
CGTAGCTGAAAGTACGTATCGTGCTTTCAAAATTGAATTTATTCATCAAGAAACCTTTCAATCGCTGGAAGAACTAGCTCTTAAGACTAAAGACTATGTT
CATTGGTGGAATTACCACCGCATTCATGGTAGTCTCAACTACCAAACACCAATGACTAAAAGATTAATTGCCTAGAAAACACTTTATAAATGTTGTACAG
AAAAGTGTTGCCTTTTCA
ATGATTTTAAGCAACAAATTGTTGACCTTTACAATGCTGGTAGAAAGCGTAGCAGTCTCATCAAGGAATATGAGCTAACCCCTTCCACCTTCGATAAGTG
GGTTAGACAAGCCAAAACAACAGGTTCATTCAAGTCTGTTGATAATCTGACAGATGAACAGCGGGAGCTGATTGAACTCAGAAAATGCAATAAAGAACTC
GAAATGCAATTAGATATCCTAAAGCAAGCGGCGGTGATTATGGCACAAAAAGGGAAATAATCACTGCTAATAAGGATAACTATAGCATTTCAGCCATGTG
TCGCTGGCTGAATATGCCACGCTCAAGTTATTACTATCAAGCCGTGGAGTCAGTATCTGAAACGGAGTTTGAAGAAACTATTAAAAGAATTTTCCTCAAG
AGCAAGTCCAGATACGGATCCAGAAAAATCAAAATATGCTTGAATAACGAAGGTATTACACTTTCACGTCGTCGGATTCGACGCATTATGAAGCGACTCA
ATTTGGTTTCTGTTTATCAGAAAGCCACCTTCAAACCACATTACAGAGGCAAGAATGAAGCCCCTATTCCCAACCATTTAGACAGGCAATTTAAGCAAGA
AAGACCACTACAAGCCTTAGTCACTGACTTAACCTATGTTCGTTTAGGCAATCGTTGGGCTTATGTTTGCCTTATTATTGACCTATACAACCGTGAAATC
ATCGGCCTGTCTCTTGGTTGGCACAAAACCGCTGAACTCGTTAAGCAAGCCATTCAAAGCATCCCTTACGCCCTGACCAAAGTCAAGATGTTCCATTCAG
ATCGTGGCAAAGAGTTTGATAATCAGTTAATTGATGAAATATTGGAAGCCTTTGGAATCACACGTTCGCTTAGTCAGGCTGGTTGTCCTTATGACAATGC
CGTAGCTGAAAGTACGTATCGTGCTTTCAAAATTGAATTTATTCATCAAGAAACCTTTCAATCGCTGGAAGAACTAGCTCTTAAGACTAAAGACTATGTT
CATTGGTGGAATTACCACCGCATTCATGGTAGTCTCAACTACCAAACACCAATGACTAAAAGATTAATTGCCTAGAAAACACTTTATAAATGTTGTACAG
AAAAGTGTTGCCTTTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 70 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MSRKIRRHFTDDFKQQIVDLYNAGRKRSSLIKEYELTPSTFDKWVRQAKTTGSFKSVDNLTDEQRELIELRKCNKELEMQLDILKQAAVIMAQKGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 294 | 1175 | + | No |
Description : Second part of the transposase
ORF sequence :
RTRNAIRYPKASGGDYGTKREIITANKDNYSISAMCRWLNMPRSSYYYQAVESVSETEFEETIKRIFLKSKSRYGSRKIKICLNNEGITLSRRRIRRIMK
RLNLVSVYQKATFKPHYRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRLGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMF
HSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFIHQETFQSLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
RLNLVSVYQKATFKPHYRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRLGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMF
HSDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFIHQETFQSLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1106 bp | 368 aa | 70 | 1175 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRKIRRHFTDDFKQQIVDLYNAGRKRSSLIKEYELTPSTFDKWVRQAKTTGSFKSVDNLTDEQRELIELRKCNKELEMQLDILKQAAVIMAQKREIITA
NKDNYSISAMCRWLNMPRSSYYYQAVESVSETEFEETIKRIFLKSKSRYGSRKIKICLNNEGITLSRRRIRRIMKRLNLVSVYQKATFKPHYRGKNEAPI
PNHLDRQFKQERPLQALVTDLTYVRLGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRS
LSQAGCPYDNAVAESTYRAFKIEFIHQETFQSLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
NKDNYSISAMCRWLNMPRSSYYYQAVESVSETEFEETIKRIFLKSKSRYGSRKIKICLNNEGITLSRRRIRRIMKRLNLVSVYQKATFKPHYRGKNEAPI
PNHLDRQFKQERPLQALVTDLTYVRLGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDNQLIDEILEAFGITRS
LSQAGCPYDNAVAESTYRAFKIEFIHQETFQSLEELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA
Blast result :
Comments
ISSdy2 is 98% (ORFA) aa similar to ISSdy1 and 92% (ORFB) aa similar to IS199. The third ORF is a putative ORF AB transposase reconstructed in sillico by -1 frameshift.
References
1] Franken,C., Brandt,C., Broker,G. and Spellerberg,B. (2004) Int. J. Med. Microbiol. 294 (4), 247-254.