ISLhe10
- Family ISLre2
- Group
Isoform Synonym(s) ISLhe15
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010080 | ND | Lactobacillus helveticus | Lactobacillus kefiranofaciens ZW3 Lactobacillus helveticus DPC 4571 Lactobacillus helveticus R0052 Lactobacillus helveticus H10 |
DNA section
IS Length : 1598 bp
Ends
IR Length : 19/24
IRL : CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAAATAATTAAAAAAGCTC
IRR : GTAAGTGTCAATATTTTTGCGGTAGGCTATTAACCATTAGTCTGACCTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGTTAAATT | AAAGTAGGC | AATCTTCTTGT | 9 |
| TGAGCGGATTA | CTGATACGA | TCAGGTCTTAG | 9 |
| CAGTTAAGATT | ATCTATCAT | CAATTTTTTGC | 9 |
| CTTTAAAATGA | AATAAGATC | CCCGAACTTTT | 9 |
| TATTTATTATA | ATATTAATC | AAAAATATTTT | 9 |
| AAATAAAAATC | GATCTAAAC | ATATTTATATA | 9 |
| AGACACTTTTT | CTGGTTAAA | TATTAACTTGC | 9 |
| AATATCCCAGC | TAAGTAGTG | TATAATATTGA | 9 |
| CTTCATAACCT | AACTGTTGC | ACTTAAATTGA | 9 |
| CGGATGATGCA | GATATTAAA | AAATATGTTGA | 9 |
| AAAAAATAATT | TTTTTGAAA | AATATGCTTGC | 9 |
| AATTTGAATAC | AAGTAGATC | TCTTTATTTGA | 9 |
| ATTATTATCG | TAAAAAACGT | 0 |
DNA sequence
CTAAGTGTATAATTTTTTGCGGTAAGTTGAATAAATAATTAAAAAAGCTCAAATCCTTTGAAATTGGACTTGAGCTCAAACAAAAAGACCAGTAGTCTGA
TGTTAACGAAAAAAACACATTAGAAGCTGGTCTTTTATGGAATCTATTATAGCTGATATCGTGAAAATTATTAAGTCTGAAAATAATGTTATTGCCCGTG
AAAAGGCATTAATGTGCTACTTCTTTGATCTGATTAGAGAATTGATGACAGCAGCACTAGAAGAAGTTGATGCTGGTCTAGTTGAAGAGACTAAAAAGCA
AGGCTATCAGATTGAAAAGAAAAATAAGCGCTCAGTTGTGACTGCCTTTGGTGAGATTAGCTATTGGCGCAGAAGATATGTCTGTCCAGGCAAGAAGGCC
CAATACCCACTGGATAAGCTGATGGGCTATGACAAATACAAGCGTTACAGTGTTTTGGCAGTAAAAGATGTTTTGCAGGTTAGCGCCGTGGCAACTTACC
GCAATACCGCTCTAGCAGTTAATACCCTTTCTTGTTTTAAGATTAGTCACAGCCAAGTTGGCAAGCTGATTGTGCAAGCAGGCAAACAGATTAAAGCACA
GCAGCAGTCTGAGGAAAGATACGATGGCATTACCCAAAAGAAAAAGGTGCCAGTGCTTTATCTTGAAGGCGATGGCGTTGTGATCAAGGGCACCAAGAAG
CGACTAGAGTTTCATCGCTATCAGGTCTGCGAGGATATTATTAATTTAAGCAAGACGCGCAGAAAGAGAGTACAGGCCAAAGAGTTTGTTTCTTTAAGCC
GCTTGGATGCTTTGCAAGAAATTAAAGCTTATTTAGCCAATGCCTATGACTTAAGCGATACTTTAATCATCAGTAATGCCGATGGCGGTGCTGGCTATGC
CAAGAAAGATTTTGATGAAATAGTTGGCTATTGCCGGCAGCACGAACACTTCTTGGATGTCTTTCACTTGAATAAAAAGATCAAGGACCGTTTAGGCTTT
ATGCCGGCTATGCAAAGCAAATTGATCAGTGCAGTTGAGTTTAAATATGACCGTCACTTAACTGATGTAATTTTAGACACGATTGAAAGCAATCTGGTTG
ATGAGCTGAATACGCCAGAAAATCATGAGAATTTAAGGCGCCTGCGCAGCTATCTTCATCGTCGCTGGGTGGATATTAAGCCATTCAAAATGCGTCATTT
GTCCGTAATTAAAGCAATTGGCTGCTGTGAGAGCAATCACCGCAAGTATACTTATCGCGTCAAAGGGCAAGGAAAATACTGGTCAGAAGATGGTGCCGAA
GGAATTCTGCGTGTACTGACTTGTATCAAAAACAATGAGCTGGAATACTGGCTTAGCAGTGAGTTTGCAGGTGGACAGCTCGATATTGGTGATCAGGAAG
ATTTGAAAGGTGCAGTTCGTGCCAGCCTAAGAAAGACGCATGGAGTTCATCTGGGCATTCATCACGGCGCCATTGAAAGCTTAACAGCAGGTCATAGCTA
TTTGGATGATTTTAGCAGAAAAATAAATCAAATAAATATTTAAGAAATTAAGGTCAGACTAATGGTTAATAGCCTACCGCAAAAATATTGACACTTAC
TGTTAACGAAAAAAACACATTAGAAGCTGGTCTTTTATGGAATCTATTATAGCTGATATCGTGAAAATTATTAAGTCTGAAAATAATGTTATTGCCCGTG
AAAAGGCATTAATGTGCTACTTCTTTGATCTGATTAGAGAATTGATGACAGCAGCACTAGAAGAAGTTGATGCTGGTCTAGTTGAAGAGACTAAAAAGCA
AGGCTATCAGATTGAAAAGAAAAATAAGCGCTCAGTTGTGACTGCCTTTGGTGAGATTAGCTATTGGCGCAGAAGATATGTCTGTCCAGGCAAGAAGGCC
CAATACCCACTGGATAAGCTGATGGGCTATGACAAATACAAGCGTTACAGTGTTTTGGCAGTAAAAGATGTTTTGCAGGTTAGCGCCGTGGCAACTTACC
GCAATACCGCTCTAGCAGTTAATACCCTTTCTTGTTTTAAGATTAGTCACAGCCAAGTTGGCAAGCTGATTGTGCAAGCAGGCAAACAGATTAAAGCACA
GCAGCAGTCTGAGGAAAGATACGATGGCATTACCCAAAAGAAAAAGGTGCCAGTGCTTTATCTTGAAGGCGATGGCGTTGTGATCAAGGGCACCAAGAAG
CGACTAGAGTTTCATCGCTATCAGGTCTGCGAGGATATTATTAATTTAAGCAAGACGCGCAGAAAGAGAGTACAGGCCAAAGAGTTTGTTTCTTTAAGCC
GCTTGGATGCTTTGCAAGAAATTAAAGCTTATTTAGCCAATGCCTATGACTTAAGCGATACTTTAATCATCAGTAATGCCGATGGCGGTGCTGGCTATGC
CAAGAAAGATTTTGATGAAATAGTTGGCTATTGCCGGCAGCACGAACACTTCTTGGATGTCTTTCACTTGAATAAAAAGATCAAGGACCGTTTAGGCTTT
ATGCCGGCTATGCAAAGCAAATTGATCAGTGCAGTTGAGTTTAAATATGACCGTCACTTAACTGATGTAATTTTAGACACGATTGAAAGCAATCTGGTTG
ATGAGCTGAATACGCCAGAAAATCATGAGAATTTAAGGCGCCTGCGCAGCTATCTTCATCGTCGCTGGGTGGATATTAAGCCATTCAAAATGCGTCATTT
GTCCGTAATTAAAGCAATTGGCTGCTGTGAGAGCAATCACCGCAAGTATACTTATCGCGTCAAAGGGCAAGGAAAATACTGGTCAGAAGATGGTGCCGAA
GGAATTCTGCGTGTACTGACTTGTATCAAAAACAATGAGCTGGAATACTGGCTTAGCAGTGAGTTTGCAGGTGGACAGCTCGATATTGGTGATCAGGAAG
ATTTGAAAGGTGCAGTTCGTGCCAGCCTAAGAAAGACGCATGGAGTTCATCTGGGCATTCATCACGGCGCCATTGAAAGCTTAACAGCAGGTCATAGCTA
TTTGGATGATTTTAGCAGAAAAATAAATCAAATAAATATTTAAGAAATTAAGGTCAGACTAATGGTTAATAGCCTACCGCAAAAATATTGACACTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1407 bp | 468 aa | 137 | 1543 | + | No |
Chemistry : DDE
ORF sequence :
MESIIADIVKIIKSENNVIAREKALMCYFFDLIRELMTAALEEVDAGLVEETKKQGYQIEKKNKRSVVTAFGEISYWRRRYVCPGKKAQYPLDKLMGYDK
YKRYSVLAVKDVLQVSAVATYRNTALAVNTLSCFKISHSQVGKLIVQAGKQIKAQQQSEERYDGITQKKKVPVLYLEGDGVVIKGTKKRLEFHRYQVCED
IINLSKTRRKRVQAKEFVSLSRLDALQEIKAYLANAYDLSDTLIISNADGGAGYAKKDFDEIVGYCRQHEHFLDVFHLNKKIKDRLGFMPAMQSKLISAV
EFKYDRHLTDVILDTIESNLVDELNTPENHENLRRLRSYLHRRWVDIKPFKMRHLSVIKAIGCCESNHRKYTYRVKGQGKYWSEDGAEGILRVLTCIKNN
ELEYWLSSEFAGGQLDIGDQEDLKGAVRASLRKTHGVHLGIHHGAIESLTAGHSYLDDFSRKINQINI
YKRYSVLAVKDVLQVSAVATYRNTALAVNTLSCFKISHSQVGKLIVQAGKQIKAQQQSEERYDGITQKKKVPVLYLEGDGVVIKGTKKRLEFHRYQVCED
IINLSKTRRKRVQAKEFVSLSRLDALQEIKAYLANAYDLSDTLIISNADGGAGYAKKDFDEIVGYCRQHEHFLDVFHLNKKIKDRLGFMPAMQSKLISAV
EFKYDRHLTDVILDTIESNLVDELNTPENHENLRRLRSYLHRRWVDIKPFKMRHLSVIKAIGCCESNHRKYTYRVKGQGKYWSEDGAEGILRVLTCIKNN
ELEYWLSSEFAGGQLDIGDQEDLKGAVRASLRKTHGVHLGIHHGAIESLTAGHSYLDDFSRKINQINI
Blast result :
Comments
ISLhe10 is 53% aa similar to ISLca4.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
ISLhe15 is an isoform of ISLhe10, the transposase is mutated with unexpected frameshift.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
ISLhe15 is an isoform of ISLhe10, the transposase is mutated with unexpected frameshift.
References