ISCbe4
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012034 | ND | Caldicellulosiruptor bescii | Caldicellulosiruptor kronotskyensis 2002 Caldicellulosiruptor bescii DSM 6725 |
DNA section
IS Length : 1610 bp
Ends
IR Length : 17/25
IRL : CTTAGTGTAAAATATTTATAGGACATTGGATGGAAAAAAGCTCCCTCCTC
IRR : GATAGTGTCAAGAGTTTGTAGGAAAAAATTTTTATCAGAATATACCTGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGCAGGATA | TATTGTGC | TGTCGCTATT | 8 |
| TCAAACCATA | ATGTTCCGC | TATCCCCTAT | 9 |
| ATAAAACTTT | TAAAAAGTAG | 0 | |
| CATTTTTTAA | GATTTGAAC | TCCGGGAGTT | 9 |
| AAAAAGTTTT | GCCAGAAG | TCCAGCAATT | 8 |
| TCTGATTTTT | CCCGCCCAA | ATACCTATTG | 9 |
| AATTAATAAA | CAGCAAAAA | CAACCCTTTG | 9 |
| ATTGGAAATT | CTCACAGGT | TGAATTGATT | 9 |
| TTTCAACTTT | GATTCGAAT | GGGAGTATTG | 9 |
| GGGTATGCAA | TTCAATAAA | AAATATAGTT | 9 |
DNA sequence
CTTAGTGTAAAATATTTATAGGACATTGGATGGAAAAAAGCTCCCTCCTCTTGGTAGAAGAGAAAAAATGAAGTAGAATATAATGATGAAAAAATAAAAA
AAGAAAAAGGAAAAACAAACCAAAGGAGGGAGCAAAATGACTGAAAATATTGTATCAAAAATAGAAGAACTTTTAAAGACTTTTGAGGAAGGATTAGAGA
AGATAGTGAAAAGGGAGAAGGATTTAGCGGAATATTCAATTGAACTTAAGAAGCAGTTAGACCTGATAGGCAAGGAGATGATAAAGGAAGCATGTGAACT
TATAGATGAATCATTGAAAGAAAACGAAGAGAGGAAGAAGAGGTATGAAGTTGTGAGGAGAGACAAGAGGGGTTTAAAGACAATATTTGGTGATATTGAA
TATGAGAGAACATATTACAAAGATAAGGAAGGCAAAGGATATGTTTATTTAGTTGATATAGTTTTAGGGATAGAGAGATATCAAAGGATAGACAATGCGG
TAAAGGGAGCGATAATAGAGAGAGTAGTAGACATGTCATATGAGAAGGCAGCAAAGGAAGTATTAGGGGAGGATAGGATTAGTAGACAAAGTGTAATGAA
TATTTTGAAAGGGATAGATAGTAAAGAATTAGATGAAATCCAGCATAGCAAGAGTGTAAAAGGAGAGAAGAAAGTTGTAAAAGAGCTTTATATCGAGGCG
GATGAAGACCATATTTCATTACAGAATGGGAAGAAAGAGATAGCGAAGCTTGCTTACATAAATACGGGATATAAGGAAGAGAAGGGCGTTGTTATTAGGA
AGGAATTAAAGGATTTGCATTATTTTAGCAGTGTAAAAGAGGATGCAGATGATTTTTGGGCAAAAGTGGGTAAGTATATTGAAGAGAATTTTGAAGTTGA
GAAGATAGAAAACATATATCTTTTAGGAGATGGAGCTAATTGGATAAAGAAGGGATTAGAGTGGATAGATGGAGCGGAATTTGTATTAGATAAGTTTCAC
TTAACGAAGGAGATAATGAAAATAGCAGGAGGGGATAGAAAATTATTTAATGAGATTTTGAAAGCATTAAGAGAGAAGGATAGAGAGGGATTTGAGGCAT
TAGTAAAAGAAAAGATGGAGGAGGTAAAAGAAGATGAGTTAGCTAAGAGGAGGATAAAGAAGGGAAGGAGATACATACTTTCGCACTGGGATAATATAGT
ATTAGAAGCAGCGAATGTTAATATAAAGGGATGTAGTGCAGAAGGGCATGTAAGTCATGTATTAGCAGAGAGGATGAGTTCAAGACCAAGAGGATGGAGC
GAAGATATAGCGGAGACCATGGTAAAGTTATTGAGTTTAAAATATAATGGTTTTGAACTTATGAATGTATATTTAGAGAAGATAAAAGCCAAGAAGTCAG
AAAGGCAAGAAGTTAAAGAAATAGCAAAAGGAATTATAAAGAACGGGAAAAGAGTACGAAAAGGTACGGAAAAGTGGAATAATATACCAGTTATAGCAAA
TGGTAAAGTTAACCAGTTACGAAGAGCATTAAAGTCTTTGATTAGTTGTAGCTTCTATAATGCAGGTATATTCTGATAAAAATTTTTTCCTACAAACTCT
TGACACTATC
AAGAAAAAGGAAAAACAAACCAAAGGAGGGAGCAAAATGACTGAAAATATTGTATCAAAAATAGAAGAACTTTTAAAGACTTTTGAGGAAGGATTAGAGA
AGATAGTGAAAAGGGAGAAGGATTTAGCGGAATATTCAATTGAACTTAAGAAGCAGTTAGACCTGATAGGCAAGGAGATGATAAAGGAAGCATGTGAACT
TATAGATGAATCATTGAAAGAAAACGAAGAGAGGAAGAAGAGGTATGAAGTTGTGAGGAGAGACAAGAGGGGTTTAAAGACAATATTTGGTGATATTGAA
TATGAGAGAACATATTACAAAGATAAGGAAGGCAAAGGATATGTTTATTTAGTTGATATAGTTTTAGGGATAGAGAGATATCAAAGGATAGACAATGCGG
TAAAGGGAGCGATAATAGAGAGAGTAGTAGACATGTCATATGAGAAGGCAGCAAAGGAAGTATTAGGGGAGGATAGGATTAGTAGACAAAGTGTAATGAA
TATTTTGAAAGGGATAGATAGTAAAGAATTAGATGAAATCCAGCATAGCAAGAGTGTAAAAGGAGAGAAGAAAGTTGTAAAAGAGCTTTATATCGAGGCG
GATGAAGACCATATTTCATTACAGAATGGGAAGAAAGAGATAGCGAAGCTTGCTTACATAAATACGGGATATAAGGAAGAGAAGGGCGTTGTTATTAGGA
AGGAATTAAAGGATTTGCATTATTTTAGCAGTGTAAAAGAGGATGCAGATGATTTTTGGGCAAAAGTGGGTAAGTATATTGAAGAGAATTTTGAAGTTGA
GAAGATAGAAAACATATATCTTTTAGGAGATGGAGCTAATTGGATAAAGAAGGGATTAGAGTGGATAGATGGAGCGGAATTTGTATTAGATAAGTTTCAC
TTAACGAAGGAGATAATGAAAATAGCAGGAGGGGATAGAAAATTATTTAATGAGATTTTGAAAGCATTAAGAGAGAAGGATAGAGAGGGATTTGAGGCAT
TAGTAAAAGAAAAGATGGAGGAGGTAAAAGAAGATGAGTTAGCTAAGAGGAGGATAAAGAAGGGAAGGAGATACATACTTTCGCACTGGGATAATATAGT
ATTAGAAGCAGCGAATGTTAATATAAAGGGATGTAGTGCAGAAGGGCATGTAAGTCATGTATTAGCAGAGAGGATGAGTTCAAGACCAAGAGGATGGAGC
GAAGATATAGCGGAGACCATGGTAAAGTTATTGAGTTTAAAATATAATGGTTTTGAACTTATGAATGTATATTTAGAGAAGATAAAAGCCAAGAAGTCAG
AAAGGCAAGAAGTTAAAGAAATAGCAAAAGGAATTATAAAGAACGGGAAAAGAGTACGAAAAGGTACGGAAAAGTGGAATAATATACCAGTTATAGCAAA
TGGTAAAGTTAACCAGTTACGAAGAGCATTAAAGTCTTTGATTAGTTGTAGCTTCTATAATGCAGGTATATTCTGATAAAAATTTTTTCCTACAAACTCT
TGACACTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1440 bp | 479 aa | 137 | 1576 | + | No |
Chemistry : DDE
ORF sequence :
MTENIVSKIEELLKTFEEGLEKIVKREKDLAEYSIELKKQLDLIGKEMIKEACELIDESLKENEERKKRYEVVRRDKRGLKTIFGDIEYERTYYKDKEGK
GYVYLVDIVLGIERYQRIDNAVKGAIIERVVDMSYEKAAKEVLGEDRISRQSVMNILKGIDSKELDEIQHSKSVKGEKKVVKELYIEADEDHISLQNGKK
EIAKLAYINTGYKEEKGVVIRKELKDLHYFSSVKEDADDFWAKVGKYIEENFEVEKIENIYLLGDGANWIKKGLEWIDGAEFVLDKFHLTKEIMKIAGGD
RKLFNEILKALREKDREGFEALVKEKMEEVKEDELAKRRIKKGRRYILSHWDNIVLEAANVNIKGCSAEGHVSHVLAERMSSRPRGWSEDIAETMVKLLS
LKYNGFELMNVYLEKIKAKKSERQEVKEIAKGIIKNGKRVRKGTEKWNNIPVIANGKVNQLRRALKSLISCSFYNAGIF
GYVYLVDIVLGIERYQRIDNAVKGAIIERVVDMSYEKAAKEVLGEDRISRQSVMNILKGIDSKELDEIQHSKSVKGEKKVVKELYIEADEDHISLQNGKK
EIAKLAYINTGYKEEKGVVIRKELKDLHYFSSVKEDADDFWAKVGKYIEENFEVEKIENIYLLGDGANWIKKGLEWIDGAEFVLDKFHLTKEIMKIAGGD
RKLFNEILKALREKDREGFEALVKEKMEEVKEDELAKRRIKKGRRYILSHWDNIVLEAANVNIKGCSAEGHVSHVLAERMSSRPRGWSEDIAETMVKLLS
LKYNGFELMNVYLEKIKAKKSERQEVKEIAKGIIKNGKRVRKGTEKWNNIPVIANGKVNQLRRALKSLISCSFYNAGIF
Blast result :
Comments
ISCbe4 is 62% aa similar to ISTte3.
References
1] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Kataeva,I. and Adams,M.W.W. (2009) Direct submission GenBank.
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761