ISCbe3
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012034 | ND | Caldicellulosiruptor bescii | Caldicellulosiruptor kronotskyensis 2002 Caldicellulosiruptor bescii DSM 6725 Caldicellulosiruptor lactoaceticus 6A |
DNA section
IS Length : 2148 bp
Ends
IR Length : 40/53
IRL : TGTTAATGATAAAATAAAAATGTACTGAAAGTGGAATATAATGATGTACA
IRR : TGTAAATGTCAATATCAAAATGAACAAAAAATCGAAAATAAAAATGTACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAATCAAAAA | GGATCTAAAT | 0 | |
| CTCAACTTCA | AAAGG | CAAAAACCCA | 5 |
| CAAGGATAAA | AGAAGG | GGGTGCAGGC | 6 |
| AAGCATGATG | CTATGAGGGA | 0 | |
| TCCCTTGCCC | CTTATAT | CACTGTCTTC | 7 |
| TTAAAAATGG | CTATAAC | ATAGTTAAGT | 7 |
| AGGGAGCGAA | ATG | TTTGATAATA | 3 |
DNA sequence
TGTTAATGATAAAATAAAAATGTACTGAAAGTGGAATATAATGATGTACAAAATTGTACATTGATATGATATCCTTTCTCATAGTGAGGAGGGATATCAA
ATGCAGAACTTAACAGCACATTTAAACATGATAAGGGCGATGAAAATGAAACCTAACTTTTCAGAACTTGCAAGAATATATGGGATGGATAGAAGAACAG
TTAAAAAATATTATGAGGGTTATGAAGGAAAACCTAAGAATAGAAATAAACCAAGTAAATTGGACAAATACTATGATGAGATAAAATCAAAGCTTGCTAT
CAAAGGAGTTACAGTCAAGGGTGTTTATGAGTATTTAAAATCAAAAGATGAGACAATAGGAACATATTCAAACTTCAATAAGTATGTTAAGAAAAAAGGA
TTAAAGCCAGAGAAGAAAATAAAAGGTCACCCAAGATTTGAGACAGATCCAGGTGAGCAAGCGCAAGTTGATTGGAAAGAGAATATAAAGCTTGTCTCAA
GAAATGGAGAGGAGTTTATCATTAATGTTCTTGATTTTAAATTAGGTTATTCAAGGTATTGCTGCTTTGAGATAAACAGGACAAAAACTCAAGAAGAATT
AATAGAAACTCTAATAAGAATATTCAAAGATATAGGCGGAGTACCGAGAGAGATTTTATTTGACAATACAGCAGCAGTTGTTGATATAACAGGTGAGAAA
ATTAAAGTAAATTCAAGATTTAAAAGTTTTGCAAAAGACTTTGGGTTTGAAGTAAAACTGTGCAAACCAAGACATTCGTACACAAAAGGAAAAGTTGAAG
CAGCAAACAAGTTTATAGATTGGATACTGCCATATCAGGGTGAATTTGAAACAGAAGAGGACTTAGTAAGGATAATAAAAGAGATAAACGCAAAGGTCAA
TATGCAGCCAAATCAAACAACTCAAGTTCCACCTGCTCTTCTGTTTCAAAAAGAAAAAGAGTATTTACAACCCTTGCCAGACAAAAGGTTAATAGACAGT
TACCTAAATTCCTACAAGTCAGTTAAAGTCCAAAAGGACTCTCTGATTTACTACAAGGGAAGTAAATACTCTGTTCCACCCGAATACATAGGAAAGACAG
TCCAAGTAAAGGAGGTGGAAAACAAAATTTATATTTATTATAACACAAACCTGTTAAGGATACATGTTATTGATGAAAAAAATATCAATTATCACGATGA
AGATTACAAGCAGCTAATGCTAATGAGAGTTGGTCAAAGAGAAGAGCTTAACAAGATATGTGAGGAAAACCTAAAGAAATTTGATAATCTGTTGAAAACC
TAAAGAAAGGAGAAAATCTAAAAATGAGCAACTATGTGAAACTACTTAACAACTTAGAAGAGTTAGGTCTTCACAACATAAAGAATAACCTTGACAAATA
CTTAGATTTAGTGGCAAGCGGAGAAAAAAGTATGACAGATGCATTATATGAACTTAGTAATTTAGAGATAAAAGCCAAAGAAGAAAGGGCGATATTAGGA
TGTGTGAGGGTGGCAAATTTCCCATTTATAAAAGGTATAGAAGATTTTGATTTTTCATTTCAGCCAAGTATAAACAAGCAACAGATAATGGACTTAATGA
GTTTGAGATTTTTAGAGGGTAATGAAAATATACTATTTGTCGGAACACCAGGGGTAGGGAAAACGCATCTAGCCACAGCAATAGGTATAGAGTGTGCAAA
ACGAAGGTATTCAACATATTTTATACATTTTCAAGAGTTAATAGCCCAGCTAAAGAAAGCATTATTGGAGAACAGATTAGAGTACAGACTTAAGCATTTT
TCGAAATACAAAGTTTTAATAATAGATGAGATAGGTTATTTGCCAATAGACAATGATGGAGCAAATTTATTTTTCCAGCTGATATCGAGCAGATATGAGA
AGAGCAGTACAATAATAACAACTAATGTTGTATTCTCAGAATGGGGAGAGATATTTGGTGGAGCGACAATAGCAAATGCAATTTTAGATAGGCTACTGCA
TCATTCTTACGTGATTTTCATAAAAGGTCCTTCATACAGATTACAGTCAAAAACAGCATATTTTAGCAATACAAACCAGCAAAGTTAAGTTTATTTTTTG
TACATTTTTATTTTCGATTTTTTGTTCATTTTGATATTGACATTTACA
ATGCAGAACTTAACAGCACATTTAAACATGATAAGGGCGATGAAAATGAAACCTAACTTTTCAGAACTTGCAAGAATATATGGGATGGATAGAAGAACAG
TTAAAAAATATTATGAGGGTTATGAAGGAAAACCTAAGAATAGAAATAAACCAAGTAAATTGGACAAATACTATGATGAGATAAAATCAAAGCTTGCTAT
CAAAGGAGTTACAGTCAAGGGTGTTTATGAGTATTTAAAATCAAAAGATGAGACAATAGGAACATATTCAAACTTCAATAAGTATGTTAAGAAAAAAGGA
TTAAAGCCAGAGAAGAAAATAAAAGGTCACCCAAGATTTGAGACAGATCCAGGTGAGCAAGCGCAAGTTGATTGGAAAGAGAATATAAAGCTTGTCTCAA
GAAATGGAGAGGAGTTTATCATTAATGTTCTTGATTTTAAATTAGGTTATTCAAGGTATTGCTGCTTTGAGATAAACAGGACAAAAACTCAAGAAGAATT
AATAGAAACTCTAATAAGAATATTCAAAGATATAGGCGGAGTACCGAGAGAGATTTTATTTGACAATACAGCAGCAGTTGTTGATATAACAGGTGAGAAA
ATTAAAGTAAATTCAAGATTTAAAAGTTTTGCAAAAGACTTTGGGTTTGAAGTAAAACTGTGCAAACCAAGACATTCGTACACAAAAGGAAAAGTTGAAG
CAGCAAACAAGTTTATAGATTGGATACTGCCATATCAGGGTGAATTTGAAACAGAAGAGGACTTAGTAAGGATAATAAAAGAGATAAACGCAAAGGTCAA
TATGCAGCCAAATCAAACAACTCAAGTTCCACCTGCTCTTCTGTTTCAAAAAGAAAAAGAGTATTTACAACCCTTGCCAGACAAAAGGTTAATAGACAGT
TACCTAAATTCCTACAAGTCAGTTAAAGTCCAAAAGGACTCTCTGATTTACTACAAGGGAAGTAAATACTCTGTTCCACCCGAATACATAGGAAAGACAG
TCCAAGTAAAGGAGGTGGAAAACAAAATTTATATTTATTATAACACAAACCTGTTAAGGATACATGTTATTGATGAAAAAAATATCAATTATCACGATGA
AGATTACAAGCAGCTAATGCTAATGAGAGTTGGTCAAAGAGAAGAGCTTAACAAGATATGTGAGGAAAACCTAAAGAAATTTGATAATCTGTTGAAAACC
TAAAGAAAGGAGAAAATCTAAAAATGAGCAACTATGTGAAACTACTTAACAACTTAGAAGAGTTAGGTCTTCACAACATAAAGAATAACCTTGACAAATA
CTTAGATTTAGTGGCAAGCGGAGAAAAAAGTATGACAGATGCATTATATGAACTTAGTAATTTAGAGATAAAAGCCAAAGAAGAAAGGGCGATATTAGGA
TGTGTGAGGGTGGCAAATTTCCCATTTATAAAAGGTATAGAAGATTTTGATTTTTCATTTCAGCCAAGTATAAACAAGCAACAGATAATGGACTTAATGA
GTTTGAGATTTTTAGAGGGTAATGAAAATATACTATTTGTCGGAACACCAGGGGTAGGGAAAACGCATCTAGCCACAGCAATAGGTATAGAGTGTGCAAA
ACGAAGGTATTCAACATATTTTATACATTTTCAAGAGTTAATAGCCCAGCTAAAGAAAGCATTATTGGAGAACAGATTAGAGTACAGACTTAAGCATTTT
TCGAAATACAAAGTTTTAATAATAGATGAGATAGGTTATTTGCCAATAGACAATGATGGAGCAAATTTATTTTTCCAGCTGATATCGAGCAGATATGAGA
AGAGCAGTACAATAATAACAACTAATGTTGTATTCTCAGAATGGGGAGAGATATTTGGTGGAGCGACAATAGCAAATGCAATTTTAGATAGGCTACTGCA
TCATTCTTACGTGATTTTCATAAAAGGTCCTTCATACAGATTACAGTCAAAAACAGCATATTTTAGCAATACAAACCAGCAAAGTTAAGTTTATTTTTTG
TACATTTTTATTTTCGATTTTTTGTTCATTTTGATATTGACATTTACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1203 bp | 400 aa | 101 | 1303 | + | No |
Chemistry : DDE
ORF sequence :
MQNLTAHLNMIRAMKMKPNFSELARIYGMDRRTVKKYYEGYEGKPKNRNKPSKLDKYYDEIKSKLAIKGVTVKGVYEYLKSKDETIGTYSNFNKYVKKKG
LKPEKKIKGHPRFETDPGEQAQVDWKENIKLVSRNGEEFIINVLDFKLGYSRYCCFEINRTKTQEELIETLIRIFKDIGGVPREILFDNTAAVVDITGEK
IKVNSRFKSFAKDFGFEVKLCKPRHSYTKGKVEAANKFIDWILPYQGEFETEEDLVRIIKEINAKVNMQPNQTTQVPPALLFQKEKEYLQPLPDKRLIDS
YLNSYKSVKVQKDSLIYYKGSKYSVPPEYIGKTVQVKEVENKIYIYYNTNLLRIHVIDEKNINYHDEDYKQLMLMRVGQREELNKICEENLKKFDNLLKT
LKPEKKIKGHPRFETDPGEQAQVDWKENIKLVSRNGEEFIINVLDFKLGYSRYCCFEINRTKTQEELIETLIRIFKDIGGVPREILFDNTAAVVDITGEK
IKVNSRFKSFAKDFGFEVKLCKPRHSYTKGKVEAANKFIDWILPYQGEFETEEDLVRIIKEINAKVNMQPNQTTQVPPALLFQKEKEYLQPLPDKRLIDS
YLNSYKSVKVQKDSLIYYKGSKYSVPPEYIGKTVQVKEVENKIYIYYNTNLLRIHVIDEKNINYHDEDYKQLMLMRVGQREELNKICEENLKKFDNLLKT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 765 bp | 254 aa | 1324 | 2088 | + | No |
AG : IS21 helper
ORF sequence :
MSNYVKLLNNLEELGLHNIKNNLDKYLDLVASGEKSMTDALYELSNLEIKAKEERAILGCVRVANFPFIKGIEDFDFSFQPSINKQQIMDLMSLRFLEGN
ENILFVGTPGVGKTHLATAIGIECAKRRYSTYFIHFQELIAQLKKALLENRLEYRLKHFSKYKVLIIDEIGYLPIDNDGANLFFQLISSRYEKSSTIITT
NVVFSEWGEIFGGATIANAILDRLLHHSYVIFIKGPSYRLQSKTAYFSNTNQQS
ENILFVGTPGVGKTHLATAIGIECAKRRYSTYFIHFQELIAQLKKALLENRLEYRLKHFSKYKVLIIDEIGYLPIDNDGANLFFQLISSRYEKSSTIITT
NVVFSEWGEIFGGATIANAILDRLLHHSYVIFIKGPSYRLQSKTAYFSNTNQQS
Blast result :
Comments
ISCbe3 is 57% (ORFA : the transposase) and 70% (ORFB : the helper of transposition) aa similar to IS232A.
References
1] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Kataeva,I. and Adams,M.W.W. (2009) Direct submission GenBank.
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761