ISCbe2
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012034 | ND | Caldicellulosiruptor bescii | Caldicellulosiruptor bescii DSM 6725 |
DNA section
IS Length : 1264 bp
Ends
IR Length : 20/28
IRL : CTCGAATAGGTGTAGAGATATTTACCACAGCGAAAGAATGTGCGAAAATA
IRR : GGCACGTGGTGAAGACATATTTACCACCTAATTTATTACAGAATAGAGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTAAAGATA | CTTATGACAATTGAG | AAAGTAATAA | 15 |
| AAGTTTTATA | ATTAGAAAGT | 0 | |
| AATCAAGAAT | TTTAGAAAGCATTGT | TTGAAATACC | 15 |
| AATCAATAGG | ACTAAAAACTGGATG | TATTTTAATT | 15 |
| TGTCAATATC | CTTTTTAAAAAATTT | TTTCTGTAAT | 15 |
DNA sequence
CTCGAATAGGTGTAGAGATATTTACCACAGCGAAAGAATGTGCGAAAATAGTGAGGAGAAACTCATAAAAAGAATTGAGGCTGCCTCCATCCTGTTTGAT
ATAATTAAAAAGTGTAATCTCCCATTCAAAAGAAAAATCAAAAAACTACCAGGATGGGAGGACAGCCTACTATGAATATTATATCATATCATGAAATGAG
AAAAATATCTCCCGAAAAAGCAAGGGAATTAATCCGAAAAGTGTTTGAAAATAATAACAAAAACGTATCAAGAACTGCTAAAATATTAGGTGTATCAAGA
CATACTGTCAGAAGAGCAATATATGGTCCCCTTGAGGATAAATCAAGAAAACCTAAAACTTGCCCCAGAAAACTTTCTACTGAACTTGAAAATCTCATTA
TCGAGGAATCTAAAAGAACTGGCTTCAGATACAGACGTTTGTCTCTTTACCTGCTCAGAAAATATGGTATTAAAATAAGCGAAAATACAATAAAGTCTAT
TCTCAAAAGAAATGCTATACCAAGAAAATCAAGAAAGACAAAAAAGGGTGAAAGAAGTTTGTACGATTATGAAGCCCTCATTCCATTTTCTGAATTTCAA
CTTGATACAAAACATCTTTTAGATAAAGACAGTCTTCCTGAAGAGGTATATGAACATATGAAAAAACACAATTTACCTTGCTATGAGTGGAACATGATAG
ACATTGCAACAAGAGCAAGATTTACAGCCTACTCCTATGAACTTTCATCTGCTTTTGGCTTTATGTTTATATCCTTGGTGGCTTTATGGCTAAGAACGCA
TAATGTAAGAAACCCAATAAAAATCCGATTGGACAATGGAGAAGAATTTTGCGGAGGAAGTGAAAGAAAGCTAAAGGAGTGGAATGAGATGTTTTCTATT
TTGGGTGTAGAACTAAATCCTATTCCACCAAGAGCAAAACATCTTATGGGGGTAATTGAAAATTCACATAGAGCAGATGACGAATATTTTTTAATGATTC
ATGCCGAAAGATGTAAAAACAAAGATGACTTTATTCAACGAGCTCAAAGGTGGCAGGATACGTGGAACTTTTTTAGACCTCATAATGGTAAAGGTATGAA
TGGGAGGACACCATTTGAAAAATTCATAGATTCTAAAACTCTGGTCTCCTCCCATGTGTTTCAGTTCCCTACTTTACTTCTTGAAGACTTATTAAAGAAA
GTAGGAACTTTTTATTCTCTATTCTGTAATAAATTAGGTGGTAAATATGTCTTCACCACGTGCC
ATAATTAAAAAGTGTAATCTCCCATTCAAAAGAAAAATCAAAAAACTACCAGGATGGGAGGACAGCCTACTATGAATATTATATCATATCATGAAATGAG
AAAAATATCTCCCGAAAAAGCAAGGGAATTAATCCGAAAAGTGTTTGAAAATAATAACAAAAACGTATCAAGAACTGCTAAAATATTAGGTGTATCAAGA
CATACTGTCAGAAGAGCAATATATGGTCCCCTTGAGGATAAATCAAGAAAACCTAAAACTTGCCCCAGAAAACTTTCTACTGAACTTGAAAATCTCATTA
TCGAGGAATCTAAAAGAACTGGCTTCAGATACAGACGTTTGTCTCTTTACCTGCTCAGAAAATATGGTATTAAAATAAGCGAAAATACAATAAAGTCTAT
TCTCAAAAGAAATGCTATACCAAGAAAATCAAGAAAGACAAAAAAGGGTGAAAGAAGTTTGTACGATTATGAAGCCCTCATTCCATTTTCTGAATTTCAA
CTTGATACAAAACATCTTTTAGATAAAGACAGTCTTCCTGAAGAGGTATATGAACATATGAAAAAACACAATTTACCTTGCTATGAGTGGAACATGATAG
ACATTGCAACAAGAGCAAGATTTACAGCCTACTCCTATGAACTTTCATCTGCTTTTGGCTTTATGTTTATATCCTTGGTGGCTTTATGGCTAAGAACGCA
TAATGTAAGAAACCCAATAAAAATCCGATTGGACAATGGAGAAGAATTTTGCGGAGGAAGTGAAAGAAAGCTAAAGGAGTGGAATGAGATGTTTTCTATT
TTGGGTGTAGAACTAAATCCTATTCCACCAAGAGCAAAACATCTTATGGGGGTAATTGAAAATTCACATAGAGCAGATGACGAATATTTTTTAATGATTC
ATGCCGAAAGATGTAAAAACAAAGATGACTTTATTCAACGAGCTCAAAGGTGGCAGGATACGTGGAACTTTTTTAGACCTCATAATGGTAAAGGTATGAA
TGGGAGGACACCATTTGAAAAATTCATAGATTCTAAAACTCTGGTCTCCTCCCATGTGTTTCAGTTCCCTACTTTACTTCTTGAAGACTTATTAAAGAAA
GTAGGAACTTTTTATTCTCTATTCTGTAATAAATTAGGTGGTAAATATGTCTTCACCACGTGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1107 bp | 368 aa | 172 | 1278 | + | No |
Chemistry : DDE
ORF sequence :
MNIISYHEMRKISPEKARELIRKVFENNNKNVSRTAKILGVSRHTVRRAIYGPLEDKSRKPKTCPRKLSTELENLIIEESKRTGFRYRRLSLYLLRKYGI
KISENTIKSILKRNAIPRKSRKTKKGERSLYDYEALIPFSEFQLDTKHLLDKDSLPEEVYEHMKKHNLPCYEWNMIDIATRARFTAYSYELSSAFGFMFI
SLVALWLRTHNVRNPIKIRLDNGEEFCGGSERKLKEWNEMFSILGVELNPIPPRAKHLMGVIENSHRADDEYFLMIHAERCKNKDDFIQRAQRWQDTWNF
FRPHNGKGMNGRTPFEKFIDSKTLVSSHVFQFPTLLLEDLLKKVGTFYSLFCNKLGGKYVFTTCPYDN
KISENTIKSILKRNAIPRKSRKTKKGERSLYDYEALIPFSEFQLDTKHLLDKDSLPEEVYEHMKKHNLPCYEWNMIDIATRARFTAYSYELSSAFGFMFI
SLVALWLRTHNVRNPIKIRLDNGEEFCGGSERKLKEWNEMFSILGVELNPIPPRAKHLMGVIENSHRADDEYFLMIHAERCKNKDDFIQRAQRWQDTWNF
FRPHNGKGMNGRTPFEKFIDSKTLVSSHVFQFPTLLLEDLLKKVGTFYSLFCNKLGGKYVFTTCPYDN
Blast result :
Comments
ISCbe2 is 96% aa similar to ISCsa6. The ends of the element are not typical of the IS481 family.
The STOP codon of the transposase is in the insertion site.
The STOP codon of the transposase is in the insertion site.
References
1] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Kataeva,I. and Adams,M.W.W. (2009) Direct submission GenBank.
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761