ISCbe1
- Family IS607
- Group
Isoform Synonym(s) ISCsa10
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012034 | ND | Caldicellulosiruptor bescii | Caldicellulosiruptor kronotskyensis 2002 Caldicellulosiruptor bescii DSM 6725 |
DNA section
IS Length : 2231 bp
Ends
IR Length : 0
IRL : TCACAACAGTTGAAAATAACAATGCAAAAAGGTATAATTTTGACCAGGTG
IRR : GGTCAACCAAACTGTGCAAAATTGCACAGTATTGGCGGCTGGGGTATTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCTTAAAGG | AGG | AGTGATTTTA | 3 |
| ATACAAAAGG | AGG | AAAGTTTTGA | 3 |
| AGCAAAGTGG | AGG | AAGGAGATTG | 3 |
| AAATGAGAGG | AGG | CTTTTATAAT | 3 |
| TTAAAGTAGG | AGG | GTAAATTAAA | 3 |
DNA sequence
TCACAACAGTTGAAAATAACAATGCAAAAAGGTATAATTTTGACCAGGTGATATTGAATGTTGCTAAGTGTGCAAAAAGTTAAAGAGATATATTCTATCT
GCCGAAGAACACTAATAAACTGGGAGAAGGAAGGGTTAATAACACCTCTCAGGACACCGAAGGGAAGGAGAAGGTATAAAAAAGAAGACATAGAAAAACT
ACTTGGCATGATAAAGGAAAAACCAAAACCAACCGTTGTTTTGTATGCAAGAGTCTCCACTAAAAAGCAAGAAGAGTACCTTAAAAACCAAATTAGAAGA
CTTGAGGAATACGCAAAGTCTCAAGGTTGGCAGTATGAAGTTATATCTGAAATAGCCAGCAAAGTAGATGAAAACAGGAGAGGACTATTAAAACTTTTGA
ACAAAATCAAAAGAGGAGAAGTTACAAAAGTTGTAATAGAGTATCCAGACAGACTTGCAAGGTTTGGATTTGAGTATCTTAAGTTTTTCATGGAGAGCTT
TGGGGTAGAACTGGTAGTGTTAAATGGCAGAGAAAATGAAGAAGATATAAACAGAGAACTGGCAGAGGACTTAATAGCGATAGTAACATCTTTTGCAGCG
AGGATTTATGGGCAAAGAGGTGCTAAAAGACATGGTAACAGTTCAGGCGAAGTTAGTGTTCGATAGAGAGGAAGACAAAAAGGCAGTATTAGATCTTATG
AGAAGATGGTCCTCTTGTATGAGGTATGCATACAAGAGACTACTGGAAAGGCATAAAAGGAATGAACTAAAAAGAGAGCTGCAAGGAATTTTTAATCTTA
ATTCCCGATACGTTGATGATGCAATAATGAAAGCAAACAGTGTTTTAAACTCATGCAAAGGAAGAGGAGAAAATCCTGAAAAGGTCATTTTTGGTGGTAG
GCAACTTTTTGAAAAACTAAAGAGGCGGTACATAAACGGCAAGGTATATAGGAAACTTCAACGAGAGTGGCAGGAGAAGAGGAAGGGGAATCTGTACTCA
AGAGGAGACAGGAGCAAAAAGGGGAATCTCAATACAAGGATTGAGATAGACGGGAACTTCACAAAACTCAGGATTAACGTAGGAAAAAGAGAGTACGTAT
ATGCGACGATACAAGCTGGATGGAAGATGAAAGGTAAGACATACATGGATAGGAACCTACTGCTACAAGCAATAAGCAGCTTTAGTGGACCTTATTCTGT
AGAACTGAAACTCAAAAACGGTGTAGTATATGCCTACTTCACCGTTGAAGAAGTTTTCCCCAAGCCTGCGATAACGAGAGCAAATGGAGTTATAGGGATA
GACACTAACGCATATCCAAAGAATGTTGCATGGGCAGAAACAGATGAGTACGGACAGTTTCTGGGATATGGCAGAATACCACTTGAGAAGCTTGAGAGTG
GAAGCTCAAGCAAGAGAGAGTATTACAGGTGGCAGTATGCACACATGATAGTACAAATGGCGAAAGAGAAGCAAAAATCGATAGTGATTGAGAACCTTAG
CATACAGGACAGGGGCAGAAGAGGCGACTTTTCAGGTAGAAAATCAAGACGGATAAGGCACTATTTTGGGTACAGGTCACTTTTGGAGAAGGTAAAACTT
CTGGCAAAGCGTGAAGGGATAGAGGTTATAGAAGTAGACCCGGCGTATACTTCTGTGATAGGGATGTTGAAGTATGCACCGCAGTATATGGTGAGCAAGG
ATATTGCGGCAGCGTATGTAATAGCGCGAAGAGGACTTGGCTTGAGAGAAAGGATACCGCACAATTATATGCTGCTTCTTAGTAGGCTTGATGTAAACAA
CCTGGAAGAGCTAAAAGAGTATGTAAGGGAGGTAGTCAAGAACAAACATCTGAGGAAAAAACAACTCAAAACGATAGATAGAGCGATAAAGTTTTTACAA
AGCTCTGGGAGTGAGCCAGGGAGGCTATCCGTGCCTCTGGATGGAACAAGCGCGGGTAGTCGTGGCAAAAAACACAATCCCTGGCAAGTTCTTAGGGTAG
CGGTGGTAACGCCACTCTCCCCTGACAGAGTCCTGCGTGATATGTCTGTCTTGAAATCGCTTTTGATTTCAGGGCAAGTGGGGAAGACCTGTAAGGGCGT
AAGTTCCTGTTTCTTGGGGCAGGGGCTATGGCTTTCCCAAATACCGCCTGCTGGGGCTGGGAAAGCCTGAAAGGCGGACTACAAATACCCCAGCCGCCAA
TACTGTGCAATTTTGCACAGTTTGGTTGACC
GCCGAAGAACACTAATAAACTGGGAGAAGGAAGGGTTAATAACACCTCTCAGGACACCGAAGGGAAGGAGAAGGTATAAAAAAGAAGACATAGAAAAACT
ACTTGGCATGATAAAGGAAAAACCAAAACCAACCGTTGTTTTGTATGCAAGAGTCTCCACTAAAAAGCAAGAAGAGTACCTTAAAAACCAAATTAGAAGA
CTTGAGGAATACGCAAAGTCTCAAGGTTGGCAGTATGAAGTTATATCTGAAATAGCCAGCAAAGTAGATGAAAACAGGAGAGGACTATTAAAACTTTTGA
ACAAAATCAAAAGAGGAGAAGTTACAAAAGTTGTAATAGAGTATCCAGACAGACTTGCAAGGTTTGGATTTGAGTATCTTAAGTTTTTCATGGAGAGCTT
TGGGGTAGAACTGGTAGTGTTAAATGGCAGAGAAAATGAAGAAGATATAAACAGAGAACTGGCAGAGGACTTAATAGCGATAGTAACATCTTTTGCAGCG
AGGATTTATGGGCAAAGAGGTGCTAAAAGACATGGTAACAGTTCAGGCGAAGTTAGTGTTCGATAGAGAGGAAGACAAAAAGGCAGTATTAGATCTTATG
AGAAGATGGTCCTCTTGTATGAGGTATGCATACAAGAGACTACTGGAAAGGCATAAAAGGAATGAACTAAAAAGAGAGCTGCAAGGAATTTTTAATCTTA
ATTCCCGATACGTTGATGATGCAATAATGAAAGCAAACAGTGTTTTAAACTCATGCAAAGGAAGAGGAGAAAATCCTGAAAAGGTCATTTTTGGTGGTAG
GCAACTTTTTGAAAAACTAAAGAGGCGGTACATAAACGGCAAGGTATATAGGAAACTTCAACGAGAGTGGCAGGAGAAGAGGAAGGGGAATCTGTACTCA
AGAGGAGACAGGAGCAAAAAGGGGAATCTCAATACAAGGATTGAGATAGACGGGAACTTCACAAAACTCAGGATTAACGTAGGAAAAAGAGAGTACGTAT
ATGCGACGATACAAGCTGGATGGAAGATGAAAGGTAAGACATACATGGATAGGAACCTACTGCTACAAGCAATAAGCAGCTTTAGTGGACCTTATTCTGT
AGAACTGAAACTCAAAAACGGTGTAGTATATGCCTACTTCACCGTTGAAGAAGTTTTCCCCAAGCCTGCGATAACGAGAGCAAATGGAGTTATAGGGATA
GACACTAACGCATATCCAAAGAATGTTGCATGGGCAGAAACAGATGAGTACGGACAGTTTCTGGGATATGGCAGAATACCACTTGAGAAGCTTGAGAGTG
GAAGCTCAAGCAAGAGAGAGTATTACAGGTGGCAGTATGCACACATGATAGTACAAATGGCGAAAGAGAAGCAAAAATCGATAGTGATTGAGAACCTTAG
CATACAGGACAGGGGCAGAAGAGGCGACTTTTCAGGTAGAAAATCAAGACGGATAAGGCACTATTTTGGGTACAGGTCACTTTTGGAGAAGGTAAAACTT
CTGGCAAAGCGTGAAGGGATAGAGGTTATAGAAGTAGACCCGGCGTATACTTCTGTGATAGGGATGTTGAAGTATGCACCGCAGTATATGGTGAGCAAGG
ATATTGCGGCAGCGTATGTAATAGCGCGAAGAGGACTTGGCTTGAGAGAAAGGATACCGCACAATTATATGCTGCTTCTTAGTAGGCTTGATGTAAACAA
CCTGGAAGAGCTAAAAGAGTATGTAAGGGAGGTAGTCAAGAACAAACATCTGAGGAAAAAACAACTCAAAACGATAGATAGAGCGATAAAGTTTTTACAA
AGCTCTGGGAGTGAGCCAGGGAGGCTATCCGTGCCTCTGGATGGAACAAGCGCGGGTAGTCGTGGCAAAAAACACAATCCCTGGCAAGTTCTTAGGGTAG
CGGTGGTAACGCCACTCTCCCCTGACAGAGTCCTGCGTGATATGTCTGTCTTGAAATCGCTTTTGATTTCAGGGCAAGTGGGGAAGACCTGTAAGGGCGT
AAGTTCCTGTTTCTTGGGGCAGGGGCTATGGCTTTCCCAAATACCGCCTGCTGGGGCTGGGAAAGCCTGAAAGGCGGACTACAAATACCCCAGCCGCCAA
TACTGTGCAATTTTGCACAGTTTGGTTGACC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 609 bp | 202 aa | 58 | 666 | + | No |
Chemistry : Serine
ORF sequence :
MLLSVQKVKEIYSICRRTLINWEKEGLITPLRTPKGRRRYKKEDIEKLLGMIKEKPKPTVVLYARVSTKKQEEYLKNQIRRLEEYAKSQGWQYEVISEIA
SKVDENRRGLLKLLNKIKRGEVTKVVIEYPDRLARFGFEYLKFFMESFGVELVVLNGRENEEDINRELAEDLIAIVTSFAARIYGQRGAKRHGNSSGEVS
VR
SKVDENRRGLLKLLNKIKRGEVTKVVIEYPDRLARFGFEYLKFFMESFGVELVVLNGRENEEDINRELAEDLIAIVTSFAARIYGQRGAKRHGNSSGEVS
VR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1539 bp | 512 aa | 632 | 2170 | + | No |
AG : TnpB
ORF sequence :
MVTVQAKLVFDREEDKKAVLDLMRRWSSCMRYAYKRLLERHKRNELKRELQGIFNLNSRYVDDAIMKANSVLNSCKGRGENPEKVIFGGRQLFEKLKRRY
INGKVYRKLQREWQEKRKGNLYSRGDRSKKGNLNTRIEIDGNFTKLRINVGKREYVYATIQAGWKMKGKTYMDRNLLLQAISSFSGPYSVELKLKNGVVY
AYFTVEEVFPKPAITRANGVIGIDTNAYPKNVAWAETDEYGQFLGYGRIPLEKLESGSSSKREYYRWQYAHMIVQMAKEKQKSIVIENLSIQDRGRRGDF
SGRKSRRIRHYFGYRSLLEKVKLLAKREGIEVIEVDPAYTSVIGMLKYAPQYMVSKDIAAAYVIARRGLGLRERIPHNYMLLLSRLDVNNLEELKEYVRE
VVKNKHLRKKQLKTIDRAIKFLQSSGSEPGRLSVPLDGTSAGSRGKKHNPWQVLRVAVVTPLSPDRVLRDMSVLKSLLISGQVGKTCKGVSSCFLGQGLW
LSQIPPAGAGKA
INGKVYRKLQREWQEKRKGNLYSRGDRSKKGNLNTRIEIDGNFTKLRINVGKREYVYATIQAGWKMKGKTYMDRNLLLQAISSFSGPYSVELKLKNGVVY
AYFTVEEVFPKPAITRANGVIGIDTNAYPKNVAWAETDEYGQFLGYGRIPLEKLESGSSSKREYYRWQYAHMIVQMAKEKQKSIVIENLSIQDRGRRGDF
SGRKSRRIRHYFGYRSLLEKVKLLAKREGIEVIEVDPAYTSVIGMLKYAPQYMVSKDIAAAYVIARRGLGLRERIPHNYMLLLSRLDVNNLEELKEYVRE
VVKNKHLRKKQLKTIDRAIKFLQSSGSEPGRLSVPLDGTSAGSRGKKHNPWQVLRVAVVTPLSPDRVLRDMSVLKSLLISGQVGKTCKGVSSCFLGQGLW
LSQIPPAGAGKA
Blast result :
Comments
ISCbe1 is 67% (TnpA : the transposase) aa similar to ISPfu4 and 39% (TnpB) aa similar to ISBth14.
References
1] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Kataeva,I. and Adams,M.W.W. (2009) Direct submission GenBank.
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761
2] Kataeva,I.A., Yang,S.J., Dam,P., Poole,F.L. II, Yin,Y., Zhou,F., Chou,W.C., Xu,Y., Goodwin,L., Sims,D.R., Detter,J.C., Hauser,L.J., Westpheling,J. and Adams,M.W. (2009) J. Bacteriol. 191 (11), 3760-3761