ISBth5

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006578 ND Bacillus thuringiensis
Bacillus thuringiensis serovar konkukian 97-27
DNA section
IS Length : 4997 bp

Ends


IR Length : 15/17

IRL : catgcccatcaacttaagaattcagaattaccccaaaatgaaaaaaagaa
IRR : catgcccatcaagctaaccaagtaaattactagataagaaacgatccttg

Insertion site


Left flankDirect repeatRight flankDR Length
ATGCATATAGGGGTAGCGTCAGAAAAATGCG11

DNA sequence

catgcccatcaacttaagaattcagaattaccccaaaatgaaaaaaagaactttttgttacaaattaacacaaaatttcgttctggaggtactgaaaaaa
ttttgcctgatagtgatggaatggggcctctataaaaaacccacagtttacattcaaactgtgggttttcgacgtacaatgatttatcaacttctgtttt
tagtctttaaaatctgcaacagaaccaggatcccccctcaaagtataaatctcaaggaggggatccgtttttttatttacttatatacgtacagctgtac
cacttacagcaaccattaacattccatcacgaactacttcgtaatctacgtcaacaccaacgatagcgttcgcacctttttgttttgcaagttctttcat
ttcatccattgcgatatcacgagcttcttttagcttactttcataagaaccagcacgaccaccgacaacatcacgaacagaagcgaataaatcgcgtaca
atgttggcccccataatagcttcaccatttacaatatcaatatactcaataatttctttaccttgaattccagaagttgttgttacaatcatgtttgaca
ccctcctaattttttataatattcaattgcatgtgtctctctatgacatatgcaattaaataaaaggaataaaattgtttaacatatgtatcgctataac
atatgcaagggatctattagaaataagatacgccgcagtaagcatcaccgtgccaggtaaataaatagcaatatccgaaagttggttcgtgtgaagaagt
gtaaaaattataatcgaaacgatggatgcaatagcagtaccaacatattccgaaaaacgatttactaatatataacgaaatataatttcttcaattattg
gtcctataatcccaaatacaagtaatgtgaatataataggcatctctgcgccatttgtaaggatctgttcttggttctctggctgacctgtatttagaaa
atgcataatagaattttccacaattatcataagtaaaactaccataggaatgcttactattttcaaaattgttttctctttaaaataagtaaaactttca
ataatttctttcctaaagaaaatgattcctacaatagcaggtacagtcaaaccgatggcactaagagcaaattctgattcaactaagttgctgttagtta
aaagggatatgccaaataatatgaacaagccaatttgaagtagtgcaaacagtatataaattattagcataacaagttcttttgtattcgtttttataaa
gctcactttattacctccataatgaaattacttagaatctttgatttttcttgttgcattaggttctccttctaaaatataattatataaatgccaaacc
ggacggttcggttcgaaaactaatgatactataagtttctagttttttaaaccccttttataaattattaataaaataaaaataaaagcaaaaatattaa
gatgttcgcatagttaagaaaagggtagtttttaatgttgttgtaataattgaatatgaatttgaaaggatgtacatcatgattttagttagttcttgct
tagctggaaaaccagtaagatataatggtactgcatgtttaaacgatacaattcaaaaattaattgatgaaaagaaagcgatacctgtttgcccggagtt
attaggtggttttgttacaccgagagaaccagctgaaatcttgggcggagatggatatgatgttttagagggcaaagcaaaagtaatggaacgttccggt
aatgatgtgacggaattatatataaatggtgccatgaagactttaaatttagcacaagaaatgcaagctagccatgtagttttaaaagaatatagccctt
cttgtggcagtaaagaaatttatagtggtgagtttgttggaaaaaaggtaacaggagtcggcgtaacgactgctctattagaaagtaagggcattaaagt
tatatctgaattagaattagataaattaattaataaaatataatattagagggcaaattttaacaattcgcttaccgaaattatcaaactttattataaa
aataaatagcttgtaaaatttcaactcacgcattttaatcaaatttttttcaaaatgcgtgagatttttttgtgatgaaatcaatggtttcgcttaaatt
ttaatcaaacttaatttcaaaccaacaaattcacttattttacatggataatataatttgattaacttcttttcaaactgtattgtttgagattttcata
agatagggattgataagatatttctgttccgaacttatttatacttacaaaaaaatcaagaaaatagagggtaaacattacctagaatattaggatcgtt
ttcattaggaggattacaaagcatgactaaagttgataaggtaaaagaaaaaattattgaaacatctttatatttttttaatactaatgggataactcgc
acatccattcaggatataatgacagccactgaactaccgaaaggatctatttatcgtagattcaaaaataaagaagaaattgttcttgctgcctacgaca
aaagtggtgaaattatgtggaaccattttcataaagcgatggaaaataaaaagacagcaatcgataaaatccttgcaattttccttgtatatcaagatgc
agctaataatcctcctattgctggaggctgtccattacttaatagcgccattgaaagtactggagtatttccagaattacaaaaagcagcagcacaaggc
tatgatgatacagtaatgttaatggcctctctcataaagggaggaatagagaaacaagaacttaaagaagatatagacattatatcactcgcttcttttc
ttgcatcttcaatggagggagctattatggcaagtcgcgtatctaacgataatatacaccatcaatattttattgaacaaataaagtatcatctttactc
ttattctaaataagtgagaacttgcatcgccttcattcaatttttataaacttaaattgaataaatactcctcataaaaagtatcttctgtttatgaggg
ggagacatttttttagcagaggtagcttacagaagaaatagggttgtttaattagaagcacataaacacaaagtggcccatcacatacccacttggacct
attttatctaaaaaatggttaggcaaaatattcattagacgaatagcaattacaggtatttttgaatttggtttcagtttatcactcactgtatttacat
aatacgaaacttagggcaagtaattggaatttctgtacctagtttatggctaagtgaccaactaaagaaattcaagctaactttgtatctatataatgaa
aaaaacgctattctttttgtagaaaaatacagaaaggatggcgttttgtatgactctatcaattcaagatgagtttcatttattcgctgaagaattacag
cgatatctatccccaagcatccttcaacagctcgcacaggagacgggtttcgtaaaacgtaaaagtaaatatggcgcacgtgatttggccgctttatgta
tttggattagtcaacacgtagcaagcgattctcttactcgattatgtagtcagctctatgcaaatacagctacactcatgagtccagaaggactgaatca
acgttttaatccagcagctgtcgcatttcttcgagaagtctttacttctcttctcacacaaaaactctgttcaaatcaatcactttctgcacatatgatc
tctactttcaatcgtatccgtattttggatgcaacagtgtttcaactgcctgatcaattggctactgactatcaaggttcaggtgggagtagtaatacgg
caggcgtaaaaatccaattggaatatgatttacttagtggtcaatttcttaacgtgcagcttggaccaggaaagaataatgataaaacatacggtaccat
ctgccttgagaccgtagagaccggcgatttgtgtttacgtgatcttggttacttcgatttaggagacttacaaaccattcatgataaagaggcttactat
atctcacggttgaagttgaatacacgcatttatatcaagaaccctgaaccagaatacttcaataatggtactttaaaaaagcaaacagaatacatccagc
ttgatatgacacaaatgatgtctggtctaatccccggtgaaacgatggaaatacccgaggcatacattggccaaaataagaaacttccagcacgtgtcat
tatccatcgtttaaccgatgatcaaacacaaacacgtctgaaaaaccaagcgatacgcgaaaagaagaaaggcattgtcatgaaggataaaagtaaacgt
ttaatgggcattaatgtatatatcacgaatacgtcgctagaagaagtaccgacgaactacgtgcatccattatattcacttcgttggcagattgaaattt
tgtttaaaacgtggaagtcgctctttaaaattgatgaatgtcaaaatataaaaagagaacgcctagagtgccatttatatggacaacttatcggcattct
cctctgttcttctacgatgtttcaaatgcgtcagtttctgcttgaaaagaaaaagcaagagctgagtgaatacaaagcgatttatatgattaaagattac
tttccgttactgtttcaagcgatagccgttggcacagaagaacttttaaaaattctgcatcgtctttatctgctgctcaaaaagaacggtcgtaaatgtc
atcggtataagaagatgaccgtctttgatattctagggattgtgtataaaacgacggtgaagcatagacaatctgcctagcgaaaaaatcacgttttaat
aggcttctttagcatgcctacttttctatcaattgctagttttgaaacaaggatcgtttcttatctagtaatttacttggttagcttgatgggcatg
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
648 bp216 aa6591306+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

VSFIKTNTKELVMLIIYILFALLQIGLFILFGISLLTNSNLVESEFALSAIGLTVPAIVGIIFFRKEIIESFTYFKEKTILKIVSIPMVVLLMIIVENSI
MHFLNTGQPENQEQILTNGAEMPIIFTLLVFGIIGPIIEEIIFRYILVNRFSEYVGTAIASIVSIIIFTLLHTNQLSDIAIYLPGTVMLTAAYLISNRSL
AYVIAIHMLNNFIPFI

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
492 bp164 aa15492040+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

IEYEFERMYIMILVSSCLAGKPVRYNGTACLNDTIQKLIDEKKAIPVCPELLGGFVTPREPAEILGGDGYDVLEGKAKVMERSGNDVTELYINGAMKTLN
LAQEMQASHVVLKEYSPSCGSKEIYSGEFVGKKVTGVGVTTALLESKGIKVISELELDKLINKI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
588 bp196 aa24233010+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MTKVDKVKEKIIETSLYFFNTNGITRTSIQDIMTATELPKGSIYRRFKNKEEIVLAAYDKSGEIMWNHFHKAMENKKTAIDKILAIFLVYQDAANNPPIA
GGCPLLNSAIESTGVFPELQKAAAQGYDDTVMLMASLIKGGIEKQELKEDIDIISLASFLASSMEGAIMASRVSNDNIHHQYFIEQIKYHLYSYSK

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
1428 bp476 aa34504877+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTLSIQDEFHLFAEELQRYLSPSILQQLAQETGFVKRKSKYGARDLAALCIWISQHVASDSLTRLCSQLYANTATLMSPEGLNQRFNPAAVAFLREVFTS
LLTQKLCSNQSLSAHMISTFNRIRILDATVFQLPDQLATDYQGSGGSSNTAGVKIQLEYDLLSGQFLNVQLGPGKNNDKTYGTICLETVETGDLCLRDLG
YFDLGDLQTIHDKEAYYISRLKLNTRIYIKNPEPEYFNNGTLKKQTEYIQLDMTQMMSGLIPGETMEIPEAYIGQNKKLPARVIIHRLTDDQTQTRLKNQ
AIREKKKGIVMKDKSKRLMGINVYITNTSLEEVPTNYVHPLYSLRWQIEILFKTWKSLFKIDECQNIKRERLECHLYGQLIGILLCSSTMFQMRQFLLEK
KKQELSEYKAIYMIKDYFPLLFQAIAVGTEELLKILHRLYLLLKKNGRKCHRYKKMTVFDILGIVYKTTVKHRQSA

 

Blast result :
Comments
ISBth5 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). ISBth5 is located on plasmid pBT9727. It's copy number in Bacillus thuringiensis serovar konkukian str. 97-27 is 1. In addition to the transposase ORF, ISBth5 harbours three passenger genes that are almost identical to those of IS231L (a probable metalloprotease (ORF1), an uncharacterised conserved protein (ORF2) and a potential transcriptional regulator (ORF3), the latter displaying similarities with TetR, the inducible repressor that regulates expression of tetracycline resistance determinants).
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18