ISfinder Mirror Site

ISPph1

Family IS1634
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Pelodictyon phaeoclathratiforme	Pelodictyon phaeoclathratiforme BU-1

DNA section

IS Length : 1849 bp

Ends

IR Length : 12

IRL : CGGAAGTTCCAGGTCTATTTTTCATTAACTCTTTATAGGCAGTATTTTTA
IRR : CGGAAGTTCCAGCAATAAAAGAACGAAAAATCCCTATAACACCTTATTTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAACTCCATG	TTGTAA	ACCCCGGTGT	6

DNA sequence

CGGAAGTTCCAGGTCTATTTTTCATTAACTCTTTATAGGCAGTATTTTTACAAGGAAAAGATGTGAAAATTGTAGTCACTAAAATATTTGGAACATTATT
TTTTATATCATATATTTAAGCGTTTAGCAAACCTATGATATAGTCACAATGTACGTCGATTCCAGCAAGACCACCATTAATGGCAAAACCTACCAGCGCC
ATCTTTTTCGTGAATCCTATCGTGAAGATGGGAAGGTGAAAAATCGCACGTTGGGCAAGATCTCAAAATGTTCGGAAGGAGAGATTGCCGCCATTAAACT
TGCCCTGAAGTACAAAGACAATTTGGCAGCCCTGTTGCATATTGAGGATGTTGAACTACATGAGGGGCTTCGTGTTGGTGTGGTATATGCACTCAAAACT
CTTGCCGAGAGGCTTGGTATCAGCAAGACGCTTGGTAATACCCGCCAGGGAAAGCTTGCATTGTGGCAGATTATGGCTCGATTGATCGGGCAGGGTTCAC
GACTCGGTGCGGTCAGGATGGCAGCAAGTTATGGCGCCTGTGATGTCCTTGAATTAGAACGCTTTACAGAAGATGACCTCTATGCCAACCTGGCTTGGTT
GACGGAGCATCAGGAGCGCATTGAGCAGCAGTTATTCAAGCACAATTCAGCGGGTGCTGGCCCGGAATTGTTGCTCTATGATGTTACCTCATCATATCTT
GAAGGGATGGAGAATGTGCTTGCTGCCTTTGGCTACAACCGGGATGGCAAAAAGGGGAAAAAGCAAATCGTTATTGGTTTGTTGTGTACCGCAGATGGCG
ATCCGGTTGCTGTCCGGGTCTTTGCAGGCAATACCGGCGACAAGTCAACCGTTGCAGAGCAGATTCGCACCGTTGCCAACACTTTTGGCATTGAAGAGGT
AACGATGGTTGGCGACAAGGGTATGATCAAAACGCCGCAGGCCAAAGAGTTGACCGATGCGGGATTTTATTACATCACCTCACTCTCGAAACCCGAAATC
AGAACCCTGCTGAAGGCAGAGGTACTGCAAATGGATTTTTTCGATTCAGAGCTGTATGAGGTTGAAAATAAAACCGATGGCGTTCGATATGTGCTGAGAA
GAAATCCGGTTCGAGAGGCCGAGATGGCAAAGAATCGCCAGGAACGGGTGAAAAAAATCCAGCGTCTTGTTGAGGAGAAAAACAGTTACCTCGCCGGTTC
CCTCAAGCGTGACAAGGATGTTGCACAACGCTCTCTTCAGAAAAAGATCAGCCAGTATAAGCTCAATGATGTGCTGGAACTGACCCATCAGGAGAGGGTG
TTCACGGTAACGGTCAATGAAGAAATCCTGAAAGGAGTCGCTCTGCTGGACGGCTGTTATGTGATCAAAACCGATGTCAAGAAAGAGCTGCTTTCGACCG
AACAAGTCCATGATCGGTACAAAGATCTGGCCAAAGTGGAGCATGCATTCCGGACGTTCAAGCAAAGTCATCTTGAAATCAGACCGGTTCATGTGCGAAC
CGAAGCGAGCACTCGTGGCAATGTCTTTGCCGTTATGCTTGCCTATAAAATCGAGAGGCAGTTATCAGAACTCTGGAAAAAATGTGAATGCACGGTACCG
GAAGGAATTGATGAACTTGGCGCAATACGCAGCACAATCGTCACCCTCAAAGGGTCAAGCTGTCAGAAAATTCCCCAGTCGAAAGGATTGGCTGCTGAGT
TGCTTGCCGCTGCCGGGATTACCCTTCCTTCGGTCATTGATGCCAAAAATGTTGATGTAGTCACAAGGAAAAAACTGGCCCCGAAGCGTAAATAAAATTG
AAATAAGGTGTTATAGGGATTTTTCGTTCTTTTATTGCTGGAACTTCCG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1644 bp	548 aa	149	1792	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYVDSSKTTINGKTYQRHLFRESYREDGKVKNRTLGKISKCSEGEIAAIKLALKYKDNLAALLHIEDVELHEGLRVGVVYALKTLAERLGISKTLGNTRQ
GKLALWQIMARLIGQGSRLGAVRMAASYGACDVLELERFTEDDLYANLAWLTEHQERIEQQLFKHNSAGAGPELLLYDVTSSYLEGMENVLAAFGYNRDG
KKGKKQIVIGLLCTADGDPVAVRVFAGNTGDKSTVAEQIRTVANTFGIEEVTMVGDKGMIKTPQAKELTDAGFYYITSLSKPEIRTLLKAEVLQMDFFDS
ELYEVENKTDGVRYVLRRNPVREAEMAKNRQERVKKIQRLVEEKNSYLAGSLKRDKDVAQRSLQKKISQYKLNDVLELTHQERVFTVTVNEEILKGVALL
DGCYVIKTDVKKELLSTEQVHDRYKDLAKVEHAFRTFKQSHLEIRPVHVRTEASTRGNVFAVMLAYKIERQLSELWKKCECTVPEGIDELGAIRSTIVTL
KGSSCQKIPQSKGLAAELLAAAGITLPSVIDAKNVDVVTRKKLAPKRK

Blast result :

Comments

ISPph1 is 51% aa similar to ISMyca1. ISPph1 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-77-D(N3)-174-E(C1). Genome sequencing of Pelodictyon phaeoclathratiforme BU-1 is currently in progress. Accession number: NZ_AAIK00000000.

References

1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2]Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2006)Unpublished