ISSav2

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003155 ND Streptomyces avermitilis
Streptomyces avermitilis MA-4680
DNA section
IS Length : 1939 bp

Ends


IR Length : 22/24

IRL : CAGCCGTAGATCGTGATCTCGATGGTGAGGGGCGTCGAGGCGGCAGGTGT
IRR : CAGCCGTAGATCGTGATCTTCATGCTTGAGCGGCTTGACGCCGGTAGTGG

Insertion site


Left flankDirect repeatRight flankDR Length
GCTAATACTCTAGTACTAAC0

DNA sequence

CAGCCGTAGATCGTGATCTCGATGGTGAGGGGCGTCGAGGCGGCAGGTGTCCAGCGTGGATCATCTCGCCCGAGATCGCCCATGCCGTCGCGGGCGTCGG
CTCCTTCTCCGTCTAACCGAGTTCACCGCGACGGAGGGCGTCCCTTATCTCGCTGAGCTTCGCGGTCGTTGCCGGGGTCCGCTCCTGTGCTTGTTCAGCA
AGTCGGAGGGCATCGTCGATCTCGCTGAGCTTCCTGGAGGACAGGACGGCCGCCCGCTCTATCAGGTCGTCCCGGGACACGGTGGTCAGCCACGTGCACG
GGGTGAAGCCCGGACGCGGGAACGCGAGCCGCAGCACGCCTTCGAGCGGCAGCCCTTCACCGGCGCCGACCGTCACTTCGATGCCCAGACCGCTGATGTC
GAGGCCCGCCGGAGCGACGACCTGCATCACCTGGAACCCGGACGCGTCGTCTCCTGACAGCAGTACGACCAACCTCCGCTCGTCGAACTGGACCCACCAG
ACTTCGCCACGTTGCACAAGCCCTCCGATACACAGGACGGCAGGCAGACGCTGCGCGACCTGACGCGGTGTGGAGACGGGTGCGGCCACCGTTGATCATC
GGTGTGTGAAGACAAACAATCATGCGGTGGCCGCAGGTCACAGCGTAGACCCTGCCTGCTGGCGGGAGGCCTTCGAGGGCCTGATGAGCCGGATAGCGGG
ACGCTTCACACGGGTCGAATCCCGACGCCGGGCCCGGAAGTTGGTACTCGGCCTGTTGTCGGACCTGCCGCGCAAGAACTGCTGGACCATCGCCGAGTGG
GCCGGGGACAGGACCCCGGACGACATGCAGCACCTGCTCGAGAGGGCGAAGTGGGATGCCGACCAGGTCCGCGACGAGGTGGGTGACTACGTGGTGGAGC
ATCTGCACGACGACGAGGCGGTGCTGGTGGTCGACGAGACCGGCGACGTGAAGAAGGGCACCGACACCGTTGGAGTCCAGCGGCAGTACACCGGCACCGC
GGGCAGGATCGAGAACGCCCAGGTCGCCGTCTACCTGGTCTACGCCGGCCGCCGCGGGCACGCCGCAGTGGACCGGGAACTCTATGTTCCGCGTTCCTGG
ACCTCCGATCCCGACCGCTGCCGGGCCGCCGGCCTCGGTGACAATACGGAGTTCGCGACCAAGCCGGAGCTGGCCGCTCGCACGGTCACCCGATTCCTCG
ACGCCGGCCATCGGGCCGCCTGGGTCGCGGGAGACGAGGTCTACGGCGACAACCCGAGGCTGCGCACCGCGCTGGAGGAACGCGGCACCGGCTACGTCCT
CGCGGTGGCCTGCTCGCACGAAGTCACCACAGGCGCCGGGAGGTTTCGCGCCGACATGTTGGCCAGGAAGGTGCCCAAGAGCGCCTGGCAGAAGCTGTCC
GCAGGGGCCGGAGCCGAGGGCCACCGCTTCTACGACTGGGCCGCCATCGACCTCACCGATCCCCGGCCCGGGAGCCGGCAGCTGCTGATCCGCCGCAACC
GCAGCACCGGCGAACGTGCCTACTACCGCTGCTACTCGCCTGCCGCAGTGCCGCTGACCACCCTGGTGCGCGTCGCTGGGTCAAGGTGGCGGGTCGAGGA
GTTATTCCAGTCCGGCAAGGGCCTGGCGGCCCTGGACGAGCACCAGGTCCGCCGCTACCCGTCCTGGTCCCGCTGGGTCACCCTGGCCATGCTCGCGCAT
GCCTTCCTCGCTGTCCTCCGCGCGAACGAACACGAGGGTCATCCCTCGCCCGACGAGCTGAAACCGCTCACTTGTGACGAGATCCAACGACTGTTCATCA
CGCTCGTCAACCGGACCGTCTTTGACCCTGTCCACCGACTTCGCTGGTCCGTCTGGCGACGCCGCCACCAGGCCCGATCCCAGACCAGCCACTACCGGCG
TCAAGCCGCTCAAGCATGAAGATCACGATCTACGGCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1233 bp411 aa6841916+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRIAGRFTRVESRRRARKLVLGLLSDLPRKNCWTIAEWAGDRTPDDMQHLLERAKWDADQVRDEVGDYVVEHLHDDEAVLVVDETGDVKKGTDTVGVQR
QYTGTAGRIENAQVAVYLVYAGRRGHAAVDRELYVPRSWTSDPDRCRAAGLGDNTEFATKPELAARTVTRFLDAGHRAAWVAGDEVYGDNPRLRTALEER
GTGYVLAVACSHEVTTGAGRFRADMLARKVPKSAWQKLSAGAGAEGHRFYDWAAIDLTDPRPGSRQLLIRRNRSTGERAYYRCYSPAAVPLTTLVRVAGS
RWRVEELFQSGKGLAALDEHQVRRYPSWSRWVTLAMLAHAFLAVLRANEHEGHPSPDELKPLTCDEIQRLFITLVNRTVFDPVHRLRWSVWRRRHQARSQ
TSHYRRQAAQA

 

Blast result :
Comments
ISSav2 is 56% aa similar to IS1452. ISSav2 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-120-E(C1). The copy number in the Streptomyces avermitilis MA-4680 chromosome is 1.
The transposase upstream region harbors 3 ORFs. One shows 87 % aa similarity with a PemK-like protein. PemK is a growth inhibitor in E. Coli known to bind to the promoter region of the Pem operon, auto-regulating synthesis. This Pfam family consists of the PemK protein in addition to ChpA, ChpB and other PemK-like proteins.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. (2003) Nat. Biotechnol. 21 (5), 526-531