ISfinder Mirror Site

ISRba4

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_005027	ND	Rhodopirellula baltica	Rhodopirellula baltica SH 1

DNA section

IS Length : 1543 bp

Ends

IR Length : 17/20

IRL : TACTACAGCGCTAAATGGTGTTGTAGAGGGCCGGGCTTTTCGCTGAGAAT
IRR : TACTACAGCGCTAAGTTTTGGGCCATCGGCATCGTGTGATGCTTTCGGGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCACGCTGC	TTAG	CTAAGCGGCG	4
TTGCCCGACC	CTAA	CTCTGTCGCA	4
CAATGCCTAC	CTAG	CAGAACCATC	4
AACGAATTGC	CTAG	CAGGCTGTTG	4
GGACGCATGC	TTAG	GCGACTTGCT	4
GACATCTTTC	CTAG	ATCGCTCTTT	4
ACCGGCCCAG	TTAG	AACCTATCCG	4
CTCCGCCTAC	NTAG	TAGTTGATCT	4
GCAACCAAGT	CTAG	GAGTGTCGGA	4
ACGGACAACT	TTAG	ATCGTAAACA	4

DNA sequence

TACTACAGCGCTAAATGGTGTTGTAGAGGGCCGGGCTTTTCGCTGAGAATAGCAGCTGGAGATTTTGTTTGCTGCACGGAGAGCTTGGCATGGATGTCGC
TGAACTGGAACAACTCGAAGATCGGTTAAATGCTTACTTGGCTCGCTTTGGCGATTGTTTTCGACGAAGCGACACGAGGGCTCATTTGACGACCTATGTC
CGTGGTCAACTTTCCGACCTGGACGCCAAGAGTGTGGAGCCGATTGCGTTGCAAGCCGGTACACCGGTGCGAACCTTGCAGGAATTTATCGCCCAGCATC
GGTGGGACGAAGATGGACTTCGCAGGAGGCTGATCCACATCGTCCGTGATGAGCATGTCAACAAGAACACTGTCGCGATCATTGACGAAACCAGCGACGT
CAAGAAGGGCGACAAAACGCCTGGCGTGCAACGACAGTGGTGCGGCAAAGTCGGCAAGCAGGAGAACTGTATCGTCACGGTTCATCTGGCTGCGGCGAAC
GAAGACTTTCACTGCATGGTCGATGGTGAACTGTTCCTCCCCGAGAGCTGGAGTAACGACCGCGAGCGTTGTGCCGCCGCCGGCATTCCCGATGAGATGG
TCTATCGCCCCAAGTGGCAGATCGCGTTGGAATTGCTTGATCGCAGTAAGGAGGAGGGGATTGAATATCCTTGGCTAACCGCTGACGAAGGCTACGGCGG
TAAACCTGGATTCCTGGAAGCTCTTGCCGACCGCGATCAGAAGTTTGTGCTTGAAGTGCCGCGAACGTTTTCGGTTTGGGAGAAGCATCCCGAAGTGACC
GAGCAGCCCTATCGCAAGGGCGGCCGCGGCCGAGGTCGCAAGACACCCCGCGTCAAGAGCGGGGAAAGTTCGCCGCGAAGTGTTGAAACAGTGTTCTGGC
ACGGCGAAGCGATGAAAGCGAAACGCTGGAAACGCTACCGCGTCAAAGACGGCGAGAAAGGTCCCATCATCTGGGAAGCCAAGCGGGTTCGCGTCACACT
CAAAGGCAGCGACGGACTACCGGGGCTGTCTCTGTGGTTGGTGGTCGCGAGAAACGTGCTTGACGGCGAACTGAAATTCTTCGTCAGCAACGCGAGCGAG
TTCGCTTCGATGGCGATGCTGCTACAGGTTGCGTTTCAGCGATGGCGAGTGGAACGTTGTTTCGAGGATCAGAAACAAGAGGTCGGCTTAGACTGTTACG
AGGGGCGCCGATATTTGGGTCTGAAACGCCACTTGATCATCACGTCGTTGAGCTATCTGTTTCTTTCGCAAACCTGCCAGCAGGAGCGGGAAAAAAAATC
CGGAGTGGACAATTCAGCAAATTCGCGACGCGGTCGACGCAATCGTTGTCAGCTGGTCACTCCCTCGTGAGAGTCGTCGCATGTTGCTTGATCAGGTCGC
CTCACGGATCAACTACCATCAATCACGCAACGTAGCGGCTCGCATCAGCCATACGCAAACGAAGCTCGACAGATACGCCGAACTCGGTATCGACCCCGAA
AGCATCACACGATGCCGATGGCCCAAAACTTAGCGCTGTAGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1278 bp	426 aa	90	1367	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDVAELEQLEDRLNAYLARFGDCFRRSDTRAHLTTYVRGQLSDLDAKSVEPIALQAGTPVRTLQEFIAQHRWDEDGLRRRLIHIVRDEHVNKNTVAIIDE
TSDVKKGDKTPGVQRQWCGKVGKQENCIVTVHLAAANEDFHCMVDGELFLPESWSNDRERCAAAGIPDEMVYRPKWQIALELLDRSKEEGIEYPWLTADE
GYGGKPGFLEALADRDQKFVLEVPRTFSVWEKHPEVTEQPYRKGGRGRGRKTPRVKSGESSPRSVETVFWHGEAMKAKRWKRYRVKDGEKGPIIWEAKRV
RVTLKGSDGLPGLSLWLVVARNVLDGELKFFVSNASEFASMAMLLQVAFQRWRVERCFEDQKQEVGLDCYEGRRYLGLKRHLIITSLSYLFLSQTCQQER
EKKSGVDNSANSRRGRRNRCQLVTPS

Blast result :

Comments

ISRba4 is 48% aa similar to ISRso17. ISRba4 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-155-E(C1). The copy number on the Rhodopirellula baltica SH 1 chromosome is 10.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Gloeckner,F.O., Kube,M., Bauer,M., Teeling,H., Lombardot,T., Ludwig,W., Gade,D., Beck,A., Borzym,K., Heitmann,K., Rabus,R., Schlesner,H., Amann,R. and Reinhardt,R. (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (14), 8298-8303