ISNpu6

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Nostoc punctiforme
Nostoc punctiforme PCC 73102
DNA section
IS Length : 1457 bp

Ends


IR Length : 25

IRL : TTATCCCTCTTCTGTCACTTTCCGGGTATTCTGAATAAAAGAATAAAAAA
IRR : TTATCCCTCTTCTGTCACTTTCCGGAATAAGCAAGTAGTAAAGAAGCTAA

Insertion site


Left flankDirect repeatRight flankDR Length
GACGGTAAGGGTAAGTCGGT0

DNA sequence

TTATCCCTCTTCTGTCACTTTCCGGGTATTCTGAATAAAAGAATAAAAAAAGAAAACCTTGAAAGGTAAGTGGGGCAATGGATGTAGAGTTACAAATACT
GAAACATTTGGCAAGAGATGCCCACCCAACAGTTGCCATCATAGATGAATATTGTGCAGATTATAAAGACCTATTTAAGGAAGTAAGAAATTATGAGTGT
TTCAAATATTTACACTTAGGAATCATATCAACAATAAAAAGGAAATCATTACCAGAGATAGCGAAGGTAGTAAGTATAAACTCAGCCCAATCATTACATC
ATTTCCTGGCAAATTCAGATTGGTCAGTAGATGAATTAAAACAACGAAGATTAAATAAACTTAAGCAAGTATTGAATGGTCAGGCGATTACAGTAGTAAT
AGATGAAACTGGAGATAGAAAAAAAGGTAAAAAGACTGACTATGTGGCGAGACAATACTTAGGAAGTGTGGGAAAAATTGATAATGGAATAGTTTCAGTC
AATGCTTATGGAGTTTACTCTAATATAACCTTTCCATTAATGGTAAAAGTGTTTAAACCAAAAGGGACGCTGAAAGAGGGAGATAAATATAAAACTAAAA
TAGAGTTAGCGTCAGAAATGATTACAGAGTTAATGGAAGAGGGCTTTCAGATTGAACTGGTACTGGCAGATAGTTTATATGGTGAAAGTAGCCAATTTAT
TAGAAAACTGGATGAATATAACTTAGCCTATGTGGTAGCAATTAGAAGTAATCATGGAGTCTGGTTGCCAGCAGGGCAAAGTGTTAGAGCAAACAATTGG
TGTAAATTTGAGAGAACATTTAGTAATCAAAAATCAGAGACTAGATACATTAGAGAAATAATTTATGGTAAAAAAAGAACCATAACTTACTGGGAAATAA
CTACTGACCCAGAAACCATGCCAGAAAATTCTACCTCCTTCGTGATGACGAATCTTCAAGGGAATTTGAAAAAGACTTTAGGCGACTTATATGGATTAAG
AACCTGGGTTGAATATGGTTTTCGACAGTGTAAACAAGAACTAGGCTGGACAGATTATCGTTTCACAAATTTTCAACATATTGAGAGGTGGTGGGAGATT
ATTTTTTGCGTTTACACGATGATTAGTTTGAATTCTCCAGCCTTGTTAGCCTTAAATCAATCTCGCCAAATTGAGGCTGAGGGGCAAAGAACTAGTCATA
TTGACTTTTCTAATCATCAACAATGGAATCATGAATCTGGATGGAAGAATGTTTTAAATAATCTGCGTCTAATCGTCCAACCACTCTTACTATTTTGGTT
GATTTATCCTTGGCTCGATATTTTCCCTAATTCACAGTTATTACTGGGATTCAATCATTTAATTGCAGCCATGAATCAATTTAAACCCTGTTATGCTTCT
GGATGATTTAGCTTCTTTACTACTTGCTTATTCCGGAAAGTGACAGAAGAGGGATAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1326 bp442 aa781403+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDVELQILKHLARDAHPTVAIIDEYCADYKDLFKEVRNYECFKYLHLGIISTIKRKSLPEIAKVVSINSAQSLHHFLANSDWSVDELKQRRLNKLKQVLN
GQAITVVIDETGDRKKGKKTDYVARQYLGSVGKIDNGIVSVNAYGVYSNITFPLMVKVFKPKGTLKEGDKYKTKIELASEMITELMEEGFQIELVLADSL
YGESSQFIRKLDEYNLAYVVAIRSNHGVWLPAGQSVRANNWCKFERTFSNQKSETRYIREIIYGKKRTITYWEITTDPETMPENSTSFVMTNLQGNLKKT
LGDLYGLRTWVEYGFRQCKQELGWTDYRFTNFQHIERWWEIIFCVYTMISLNSPALLALNQSRQIEAEGQRTSHIDFSNHQQWNHESGWKNVLNNLRLIV
QPLLLFWLIYPWLDIFPNSQLLLGFNHLIAAMNQFKPCYASG

 

Blast result :
Comments
ISNpu6 is 73% aa similar to IS701. ISNpu6 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-88-D(N3)-113-E(C1). The chromosome sequencing of Nostoc punctiforme PCC 73102 is currently in progress. Accesion number: NZ_AAAY00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] NCBI Microbial Genomes Annotation Project (2006) Unpublished