ISNha2

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007964 ND Nitrobacter hamburgensis
Nitrobacter hamburgensis X14
DNA section
IS Length : 1455 bp

Ends


IR Length : 18

IRL : CACTACTTTGGCAGATTTTTGCGATTGATGGGCGCAACTGGATTCCCGAA
IRR : CACTACTTTGGCAGATTTATTCACGCCGCTGCTTTTCGCCGATTTGTCTT

Insertion site


Left flankDirect repeatRight flankDR Length
AGTCCCCTTTCTAGGATCAGCGAA4

DNA sequence

CACTACTTTGGCAGATTTTTGCGATTGATGGGCGCAACTGGATTCCCGAATCAGATTTTCAATGATTCATGGGTGGTCGGCTTTTGGAGGGCCGACCATG
GTGGACACGAGGTCGAAGTGGGAAGACGAGCTTGGACGCTGGCTCAAGCCATTCCTGGATCGTTTAGGTCACAAGGCCCGGCGGCGGATGTGTCCGCTGT
ATGTTTCGGGACTGATTGGACCGGGTGATCGCAAGAGCGTCCAGCCGATGGCGGCGCGGCTGGCACCGAACGACTATGACCAGTTGCACCATTTTATCGC
TGATGGCGTTTGGGATGCGGCGCCATTGGAGTCAGAATTGCTGATTCAGGCCGATCGCCTCGTCGGCGGCAAAGATGCGGTGCTGGTCATTGACGACACG
GCGATGCCGAAGAAGGGCGATCGTTCGGTTGGTGTCGCTCCGCAATATGCCTCGTCGCTCGGCAAGACGGCCAATTGCCAAACATTGGTGTCGCTGACGC
TTGCGCGGGGTGAAGTGCCGGTCATGGTGGCATTACGTCTCTTCGTTCCCGAGAGTTGGACGAGCAATCCGGTGCGTTTGAAGCGTGCGGGCGTGCCAGT
CGAGCACCGCGCAGCGCGGACCAAGCCAGAGATCGCCTTGGCGGAGATCGATCGCGTGATGGCAGCCGGTATGCGCTTCGGCTGTGTGCTGGCGGATGCC
GGTTATGGCCTCAGCGCGCCGTTCCGTCAGGGGCTCACGACACGCGGCCTGGCCTGGGCCGTCGGGATCCCTCGTCACCTGAAGGTGTATCCGGTCGGGG
TTAAACTGATCTGGCCGGTTGCCGGTCGGGGCCGTCCCCGCAAGCGGCACATTCCCGATATCCTATCGAGGGCGGCCGAAGATATGCTGGCCAATGCCAA
GTGGCAAAATGTGAGTTGGCGAAACGGTACCAAGGGTCGGCTGGAAGCTCGCTTCGCCGCTGTTCGCGTGCGAACCGCCGATGGGCCACCTCAGCGGATC
AAGGACATGGGCCAGCAGCATCTCCCGGGGGACGAAGCCTGGCTCATCGGCGAACACAGGACGTCGGGAGAGAAGAAATATTATCTCGCCAATCTGCCGG
CCAAGACGAAGCTGCGCGCGTTAGCTGCCACGATCAAAGCCCGATGGATTTGCGAACAGGCCCATCAGCAGTTGAAAGAGGAACTCGGGCTTGATCACTT
CAAGGGAAGATCCTGGCAAGGCCTCCATCGTCACGCGCTCATGACCATGATTGCTTACGCATTCCTCCAGCATCGCCGTCTCGCACAAGCGGGGCGGAAA
AAAAAGAATCAACGGTCCACCGCCTCAGCCGAGCCTGCCGGCCGTACGCCAAGCCATCGTCGATCTCATCGCTCAACCACCACCTCAGCGGTGCGCATAC
TGCCGAAGACAAATCGGCGAAAAGCAGCGGCGTGAATAAATCTGCCAAAGTAGTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1335 bp445 aa981432+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVDTRSKWEDELGRWLKPFLDRLGHKARRRMCPLYVSGLIGPGDRKSVQPMAARLAPNDYDQLHHFIADGVWDAAPLESELLIQADRLVGGKDAVLVIDD
TAMPKKGDRSVGVAPQYASSLGKTANCQTLVSLTLARGEVPVMVALRLFVPESWTSNPVRLKRAGVPVEHRAARTKPEIALAEIDRVMAAGMRFGCVLAD
AGYGLSAPFRQGLTTRGLAWAVGIPRHLKVYPVGVKLIWPVAGRGRPRKRHIPDILSRAAEDMLANAKWQNVSWRNGTKGRLEARFAAVRVRTADGPPQR
IKDMGQQHLPGDEAWLIGEHRTSGEKKYYLANLPAKTKLRALAATIKARWICEQAHQQLKEELGLDHFKGRSWQGLHRHALMTMIAYAFLQHRRLAQAGR
KKKNQRSTASAEPAGRTPSHRRSHRSTTTSAVRILPKTNRRKAAA

 

Blast result :
Comments
ISNha2 is 52% aa similar to ISRso17. ISNha2 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-100-D(N3)-152-E(C1). The copy number in Nitrobacter hamburgensis X14 is 1.
References
1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2]Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ward,B., Arp,D., Klotz,M., Stein,L., O'Mullan,G., Starkenburg,S., Sayavedra,L., Poret-Peterson,A.T., Gentry,M.E., Bruce,D. and Richardson,P. (2006)Unpublished