ISfinder Mirror Site

ISMmg4

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007626	ND	Magnetospirillum magneticum	Magnetospirillum magneticum AMB-1

DNA section

IS Length : 1507 bp

Ends

IR Length : 16

IRL : TTCTACTGTGTCATAAAACAAGCCATTGTGGTAGCAATGGAGATTCGGTC
IRR : TTCTACTGTGTCATAAGCATGAGGATCTGGGGTGTCGGCCGCTGGCAGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGCCACCGGC	GTAG	CACCTCGGAG	4

DNA sequence

TTCTACTGTGTCATAAAACAAGCCATTGTGGTAGCAATGGAGATTCGGTCTGCTCAATAGGGGGGTGAGCATGGGTCTCCATGGAAAGGCTGGCGATAGT
GAATCGCGATTTGAGGCGTATGTCGAGGGCCTTTGCCGTTCGGTTCGCCATGCCGACCGGGCGGCGCCGTTGCGGGCGTATTGCCTCGGGTTGATGCTGC
CGGGCGACCGCAAGAGCGTCGAGCCGATGGCGGCGCGGGTCGAGCCGGGACGGGTGCAGGCGGCGCACCAGTCGATGCATCACTTCGTCGCCAAGGCCGA
GTGGTCCGACGAGGCGATGCTTGCGGCGGTACGGGCGACGGTGCTGCCGGCGATCACCCGGCATGGCCGGCTCCGCGCCTGGATTATCGACGACACCGGC
CTTCCCAAGAAGGGCGTTCATTCGGTGGGTGTAGCGCGGCAGTATTGCGGCCAACTCGGCAAGCAAGACAATTGCCAGGTGGCGGTGACGCTATCGATCG
CCAATGATCACGCCAGCCTGCCGATCGCCTATCGCCTTTATCTGCCAGAAGACTGGGCCTCGGACCCGGCGCGGCGCCACAAGGCGGGGGTGCCGGACGA
GGTCATGTTCCACACCAAGCCGCAGATCGCGCTGGAGCAGGTCCGCGCCGCCCTGGCCGAGGGCGTGGCGCCCGGAGTGCTGTTGGCCGATGCCGGATAC
GGCGTCGATACCGCCTTTCGCACCGGCTTGTCCCGGCTCGGCCTGACTTACGTCGTCGGCATTCAGTCCTCCACCAGCTTATGGAAGCCGGGCGAGGCGC
CATTGCCGCCCAAGCCGTGGAGCGGCAGGGGACGACCACCCTCACTGGTCCGCCACGACCCCGAGCATAAGCCGGTTTCGGCCAAGGAGATGGCGACCGC
CTTGCCGCCCACGGCGTGGCGCACGGTGACTTGGCGCGAGGGGACCAACACCCTGCTGAGTTCACGCTTTGCCGCCGTCCGGGTGCGCCCCGCCCACCGC
GACACCTTGCGCTCCGAGCCCAGGCCCGAAGAATGGTTCTTGGTCGAGTGGCCCGAGGAAGACGCCGAACCGACCAAGTACTGGTTCTCCACCCTGCCCG
CCGACATACCGCTGACCAATCTCGTCGAGATCACCAAATTGCGCTGGCGCATCGAGCGCGATTTTCAAAATCTCAAGCAGGAACTTGGCCTTGGCCACTA
TGAGGGCCGAGGGTGGCGAGGATTCCACCACCACGCAGCCCTCTGCGTCGCCGCCTACGGATTCCTCATCATCGAGAGGAGTCTGATTCCCCCCTCGGGA
CTTTGCTCCGCCCCGATCCGCCAAGCGCCTGCCCTACCCGACGGTTACCGACCCCGAGGATCCGCCCGTTCGGCCTGAGCGCCACGTGACAACGTCGATC
ACAACGATACGGCTGAGACTGACACGGGCGCTTACCCGACGATTACCACGGTGCCCCTGCTGCCAGCGGCCGACACCCCAGATCCTCATGCTTATGACAC
AGTAGAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1305 bp	435 aa	71	1375	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGLHGKAGDSESRFEAYVEGLCRSVRHADRAAPLRAYCLGLMLPGDRKSVEPMAARVEPGRVQAAHQSMHHFVAKAEWSDEAMLAAVRATVLPAITRHGR
LRAWIIDDTGLPKKGVHSVGVARQYCGQLGKQDNCQVAVTLSIANDHASLPIAYRLYLPEDWASDPARRHKAGVPDEVMFHTKPQIALEQVRAALAEGVA
PGVLLADAGYGVDTAFRTGLSRLGLTYVVGIQSSTSLWKPGEAPLPPKPWSGRGRPPSLVRHDPEHKPVSAKEMATALPPTAWRTVTWREGTNTLLSSRF
AAVRVRPAHRDTLRSEPRPEEWFLVEWPEEDAEPTKYWFSTLPADIPLTNLVEITKLRWRIERDFQNLKQELGLGHYEGRGWRGFHHHAALCVAAYGFLI
IERSLIPPSGLCSAPIRQAPALPDGYRPRGSARSA

Blast result :

Comments

ISMmg4 is 73% aa similar to ISRso17. ISMmg4 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-154-E(C1). The copy number in Magnetospirillum magneticum AMB-1 is 1.

References

1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2]Matsunaga,T., Okamura,Y., Fukuda,Y., Wahyudi,A.T., Murase,Y. and Takeyama,H.(2005)DNA Res. 12 (3), 157-166