ISMba8

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007355 ND Methanosarcina barkeri
Methanosarcina barkeri fusaro
DNA section
IS Length : 1646 bp

Ends


IR Length : 19/24

IRL : CTTGACTTTGTCACATTTCCTTTTCACATCAACCGTGAAGTCCTAATTTT
IRR : CTTGACATTGTCAGATTATCTCTTTTTTGGAGTTGTTACTACATATTTTT

Insertion site


Left flankDirect repeatRight flankDR Length
TCTATCTTTCCTATATACGGTTCA4
TCAGGATTTCNTAATTAATAGATG4

DNA sequence

CTTGACTTTGTCACATTTCCTTTTCACATCAACCGTGAAGTCCTAATTTTTCCTCTATAAATTTGGTTTAGTGAAACAAATAACAGATGATTTTATCAAG
TCAAATAATATTTCTGTAGAAAAATACATTTCAGAGAAACATCTTGAATCCTATTTCCAAACGAAAACTCGTTGTGTTGTTTCAACCGGCATTGATTACC
TTAATGGTTTGTTTCTTCTCCCCTATAGGAAAAATATGAGAAAAATGGCAATCTATTGTTGTAAATGCTCTAACAACCAATCACTAAGCCATTTTATCTC
AACTTCTCCTTGGCGTCCTGCAGATCTCCTCAAATCTGTTCGAACATATGCCATTAAAACTATAGGCAAAAATGGTGTTCTTATACTTGACGAATCAAGC
ATAAAAAAATCTGGAAATAGTTCTGTTGGAGTTTCTCATCAGTATTGCGGCAACCTTGGAAAAAAGAATAATTGTCAGGTTGGTGTGTTCCTTGCTTATT
TTAAGAAACAGAAGAGAATGTTGATTGATGAGAGATTATATCTCCCACAAAAATGGATTAATGACAAGGCACGTTGTTTAAAGGCTAAAATTCCTCCTGA
GAAAATAAAGTTCCGAACCAAGCATGAGCTTGGCCTTGAAATGATTGATAACGCAATAGAGGAAGGTATTCCTTTTTCCTATGTTACAATGGATGGATTC
TACGGTGAAAATCCGGAGTTATTGACAGAACTTGAAAATAGAGGCTTAACTTTTGTTGCAGATGTAGCGGTCGATACTCTTGTTTATATTGAGGAACCAT
TAGTTGGGATTCCTGAAAAAAAGGGAACAAGAGGAAGAAAACCCACAATTTCTAAGGTTTTGAATACATTATCAACAAGAATTGATTCTCTTTCCAGTTC
AATTGATGGATGGAAACTTATCAAAGTTAGAAAAACGGAAAGAGGGTACAAAAAAGTCTATTTTAAGGCTATAAAAGTTTGGAGACGTCAAAATAAATTA
CCTTGTGAGAAGCCATTATGGCTTCTCATAAGTAAAGATGTAGAGTCTAATGACATTAAATATTCATTATGTAACGCTTCTGAGGATACTTCATTAGACG
AACTTGCAAAAATGCAGAGCTCAAGGTACTGGATTGAGAGAGCATTTCAAGATGCAAAAGTATATTGTGGAATGTCTGAATACATGGTGAGAAGCTGGAA
TGCATGGCATCACCATATGGCATTGGTTATGCTTGCGATGCTTATTCTGTTGTCGTACAAAATAAAATTTATGAAATTTCATAAGATTTCACTTCATGGT
GTCATTTTGATTATGAAATTCCATAATCCATTGAAGATATTAAATGCGGGAGAGCTTGCAAGAATATTAAATCATGATAATGAGATGAGAGACAGAGCAA
GAGCAAGTAGATTGAAAGGTTCTTGACAAAAAACATGAGTAGGATTTTACTGAATTTTCGTTCTGGTTACTGATAAGTATGCAATTTTGAAAGTGAAAAT
GAAGGACATTACAAATAATTACTGTATTGATCAAGGTGAATAATGAGAAAACAAAAATGTCGAGTCAAAAATATTCAAAATAGGGGAATCAATTGGAAAA
ATATGTAGTAACAACTCCAAAAAAGAGATAATCTGACAATGTCAAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1188 bp396 aa2361423+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKMAIYCCKCSNNQSLSHFISTSPWRPADLLKSVRTYAIKTIGKNGVLILDESSIKKSGNSSVGVSHQYCGNLGKKNNCQVGVFLAYFKKQKRMLIDER
LYLPQKWINDKARCLKAKIPPEKIKFRTKHELGLEMIDNAIEEGIPFSYVTMDGFYGENPELLTELENRGLTFVADVAVDTLVYIEEPLVGIPEKKGTRG
RKPTISKVLNTLSTRIDSLSSSIDGWKLIKVRKTERGYKKVYFKAIKVWRRQNKLPCEKPLWLLISKDVESNDIKYSLCNASEDTSLDELAKMQSSRYWI
ERAFQDAKVYCGMSEYMVRSWNAWHHHMALVMLAMLILLSYKIKFMKFHKISLHGVILIMKFHNPLKILNAGELARILNHDNEMRDRARASRLKGS

 

Blast result :
Comments
ISMba8 is 46% aa similar to ISRso17. ISMba8 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-100-D(N3)-147-E(C1). The copy number in Methanosarcina barkeri str. fusaro is 2.
References
1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2]Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A., Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Anderson,I. and Richardson,P.(2005)Unpublished